##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939581_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1843954 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.551953031366292 31.0 31.0 34.0 30.0 34.0 2 31.72856101616418 31.0 31.0 34.0 30.0 34.0 3 31.76542202245826 31.0 31.0 34.0 30.0 34.0 4 35.53293032255685 37.0 35.0 37.0 33.0 37.0 5 35.28667906032363 37.0 35.0 37.0 33.0 37.0 6 35.33723509371709 37.0 35.0 37.0 32.0 37.0 7 35.27460717566707 37.0 35.0 37.0 32.0 37.0 8 35.25063315028466 37.0 35.0 37.0 32.0 37.0 9 36.82657918798408 39.0 37.0 39.0 32.0 39.0 10-11 36.71286187182544 39.0 35.0 39.0 32.0 39.0 12-13 36.6660570166067 39.0 35.0 39.0 32.0 39.0 14-15 37.60213134384047 39.0 36.0 41.0 32.0 41.0 16-17 37.60395975170748 39.0 36.0 41.0 32.0 41.0 18-19 37.6772240522269 39.0 36.5 41.0 32.0 41.0 20-21 37.626405810557095 39.0 36.5 41.0 32.0 41.0 22-23 37.48525397054374 39.0 36.0 41.0 32.0 41.0 24-25 37.31761584074223 39.0 36.0 41.0 31.5 41.0 26-27 37.0022787987119 39.0 36.0 40.5 31.0 41.0 28-29 36.81624758535192 39.0 36.0 40.0 30.5 41.0 30-31 36.558283720743574 38.0 35.0 40.0 30.0 41.0 32-33 36.32165200433416 38.0 35.0 40.0 30.0 41.0 34-35 36.24175684425967 38.0 35.0 40.0 30.0 41.0 36-37 36.061454895295654 38.0 35.0 40.0 30.0 41.0 38-39 36.022500561293825 38.0 35.0 40.0 30.0 41.0 40-41 35.93194353004468 38.0 35.0 40.0 29.5 41.0 42-43 35.7069826579188 38.0 35.0 40.0 28.5 41.0 44-45 35.4126195664317 38.0 34.5 40.0 27.0 41.0 46-47 34.98131515211334 38.0 34.0 40.0 25.5 41.0 48-49 34.56408782431666 38.0 33.5 40.0 24.0 41.0 50-51 34.25630709876711 38.0 33.0 40.0 23.5 41.0 52-53 34.00879875528348 38.0 33.0 40.0 23.0 41.0 54-55 33.73064810727382 37.5 33.0 40.0 22.5 41.0 56-57 31.734298415253306 36.0 30.0 39.5 13.5 41.0 58-59 26.786997397982812 32.5 16.5 38.0 3.5 40.0 60-61 27.462339624524255 32.0 18.0 38.0 2.0 40.0 62-63 30.466050400389598 34.5 29.0 38.0 2.0 40.0 64-65 30.65918347203889 35.0 29.5 38.5 2.0 40.0 66-67 24.133112322758592 26.5 16.5 35.5 2.0 38.5 68-69 16.89962385178806 13.0 7.0 28.0 2.0 35.0 70-71 15.775455081851284 12.5 7.0 26.0 2.0 33.5 72-73 16.869590835780066 13.0 7.0 28.0 2.0 34.0 74-75 17.854231992772057 17.0 7.5 29.0 2.0 34.5 76-77 18.98645654934993 21.0 7.0 30.0 2.0 34.0 78-79 21.969851742505508 27.5 9.0 33.5 2.0 35.5 80-81 25.424994603986868 31.0 21.0 34.5 2.0 36.0 82-83 26.684527922063133 33.0 25.5 35.0 2.0 36.0 84-85 26.735474963041376 33.0 25.5 35.0 2.0 36.0 86-87 26.546110152422457 33.0 25.0 35.0 2.0 35.0 88-89 26.120336515986843 33.0 24.5 35.0 2.0 35.0 90-91 24.807311353753946 32.5 11.0 35.0 2.0 35.0 92-93 22.443034641862 31.5 2.0 34.0 2.0 35.0 94-95 15.448895688287234 4.5 2.0 32.0 2.0 34.5 96-97 12.978156993070325 2.0 2.0 30.0 2.0 34.0 98-99 12.678401413484284 2.0 2.0 29.5 2.0 34.0 100-101 12.390844890924612 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4336868296420633 1101 2 0.40731076419125856 1101 3 0.4749560887093054 1101 4 0.282364514355919 1101 5 0.3864416824872521 1101 6 0.40467738072442927 1101 7 0.3600726407766359 1101 8 0.37305601099370733 1101 9 0.5171427641247277 1101 10-11 0.6103772042803257 1101 12-13 0.6281347438433045 1101 14-15 0.7681110634886181 1101 16-17 0.7376323302800216 1101 18-19 0.6560327093140685 1101 20-21 0.783505166151464 1101 22-23 0.8121887692442797 1101 24-25 0.7115366662091063 1101 26-27 0.6301338210799514 1101 28-29 0.6992941871882081 1101 30-31 0.7240292611494894 1101 32-33 0.7191909296103063 1101 34-35 0.49514727243710155 1101 36-37 0.4378050381364318 1101 38-39 0.5706892519403581 1101 40-41 0.7426346142945377 1101 42-43 0.6730112207617225 1101 44-45 0.6218888146245263 1101 46-47 0.5553724221592233 1101 48-49 0.5388440822471097 1101 50-51 0.5534045249928354 1101 52-53 0.3589501393638841 1101 54-55 0.16703229573599288 1101 56-57 -1.983885867900419 1101 58-59 -9.938549064731578 1101 60-61 -10.217215129896246 1101 62-63 -3.6728416988891617 1101 64-65 -4.409752928808064 1101 66-67 -9.112401650487234 1101 68-69 -4.352939948264805 1101 70-71 -4.081858217349131 1101 72-73 -4.505333083190923 1101 74-75 -6.512085274396014 1101 76-77 -7.710149106113182 1101 78-79 -10.56645573350104 1101 80-81 -7.813395089358618 1101 82-83 -3.81198985239865 1101 84-85 -3.068568823837161 1101 86-87 -2.6756453920722443 1101 88-89 -2.6175228506386965 1101 90-91 -8.885829586542659 1101 92-93 -16.147334621358134 1101 94-95 -10.661147236398232 1101 96-97 -9.107056928701983 1101 98-99 -9.111876983051593 1101 100-101 -8.961710688312664 1102 1 0.3575952886628819 1102 2 0.424079920988504 1102 3 0.3765938219481555 1102 4 0.2678223036058469 1102 5 0.3219677984908955 1102 6 0.2718822260191658 1102 7 0.4048068412692629 1102 8 0.4329254309754873 1102 9 0.37017697811399586 1102 10-11 0.4687339458533586 1102 12-13 0.47598582526180166 1102 14-15 0.5076684579617421 1102 16-17 0.5236765908327143 1102 18-19 0.49527166737325246 1102 20-21 0.5559011576485702 1102 22-23 0.6908552165904993 1102 24-25 0.47337490894622647 1102 26-27 0.4851843069590984 1102 28-29 0.5451705410701777 1102 30-31 0.47369627366762046 1102 32-33 0.569770230821625 1102 34-35 0.38325293427871543 1102 36-37 0.35460329706951654 1102 38-39 0.3425940921738544 1102 40-41 0.13073626932035154 1102 42-43 0.32532905826214886 1102 44-45 0.5165890626253287 1102 46-47 0.47253972991642 1102 48-49 0.39643109341557903 1102 50-51 0.36015579873849646 1102 52-53 0.28098846982918246 1102 54-55 0.07804703238275579 1102 56-57 -2.7053934007141365 1102 58-59 -9.916016630612067 1102 60-61 -10.477962042539229 1102 62-63 -4.312418283901142 1102 64-65 -5.1356820883046375 1102 66-67 -6.354206657741681 1102 68-69 -3.8177894185215777 1102 70-71 -3.846307006025448 1102 72-73 -4.401882793013105 1102 74-75 -5.050477950780262 1102 76-77 -7.073387203761392 1102 78-79 -9.57637007199684 1102 80-81 -5.430270532217204 1102 82-83 -3.0586218073873503 1102 84-85 -2.587003128928771 1102 86-87 -2.4805311767333116 1102 88-89 -2.6995870777871964 1102 90-91 -8.544579206949582 1102 92-93 -13.320135512136552 1102 94-95 -10.249295497018403 1102 96-97 -9.057095942434817 1102 98-99 -9.169859403674463 1102 100-101 -8.987476622786463 1103 1 0.2658251968927914 1103 2 0.24984306982857873 1103 3 0.249835433651306 1103 4 0.26978790557144805 1103 5 0.2786140628294689 1103 6 0.2526096990543252 1103 7 0.27244435506062104 1103 8 0.2310819522089318 1103 9 0.23801253825725155 1103 10-11 0.4158560545139238 1103 12-13 0.4784465431071325 1103 14-15 0.5400703595944094 1103 16-17 0.5520178960970981 1103 18-19 0.5286860930953665 1103 20-21 0.3111749898454903 1103 22-23 0.46612898417196647 1103 24-25 0.6443294568238542 1103 26-27 0.4713887470096836 1103 28-29 0.4094897284662906 1103 30-31 0.5521126136224197 1103 32-33 0.6056998129050584 1103 34-35 0.6284635564124201 1103 36-37 0.4043646237539278 1103 38-39 0.29961900304491706 1103 40-41 0.48050583447453477 1103 42-43 0.4611307017560975 1103 44-45 0.3649968571869806 1103 46-47 0.3245325511400097 1103 48-49 0.23282655288795695 1103 50-51 0.35013475025590424 1103 52-53 0.3954035303980916 1103 54-55 0.2775191241625308 1103 56-57 -2.1492658368942656 1103 58-59 -9.741875087239752 1103 60-61 -10.126607298876792 1103 62-63 -4.101040795600571 1103 64-65 -5.303367579067054 1103 66-67 -6.431877658489604 1103 68-69 -3.9047175516035573 1103 70-71 -3.9807378327639658 1103 72-73 -4.269276221944995 1103 74-75 -4.963198632731714 1103 76-77 -6.462035238563272 1103 78-79 -8.969085326250555 1103 80-81 -5.062717018509847 1103 82-83 -2.7941565890759783 1103 84-85 -2.3372136945239532 1103 86-87 -2.2091900669306597 1103 88-89 -2.1898346680347878 1103 90-91 -5.1272420742278335 1103 92-93 -10.132130724131764 1103 94-95 -8.503963236301527 1103 96-97 -7.980878143140094 1103 98-99 -8.226266590850882 1103 100-101 -8.347370944219247 1104 1 0.12908286456686113 1104 2 0.16676045641351678 1104 3 0.0057021100469825114 1104 4 0.18872490968195166 1104 5 0.2134902357546551 1104 6 0.14432754199760467 1104 7 0.07223325822248938 1104 8 0.24418811321609013 1104 9 0.15336395057541807 1104 10-11 0.15700944532758854 1104 12-13 0.1442288316751572 1104 14-15 0.35266945264727667 1104 16-17 0.31306187517067485 1104 18-19 0.24679022062681355 1104 20-21 0.19431165016001017 1104 22-23 0.2061184293300542 1104 24-25 0.16140315801548866 1104 26-27 0.34278512544319284 1104 28-29 0.44713575549595674 1104 30-31 0.3328838524135662 1104 32-33 0.10991100250814867 1104 34-35 0.1930709541913771 1104 36-37 0.002196158069324383 1104 38-39 -0.07976407040512612 1104 40-41 -0.14397314040483167 1104 42-43 -0.039487966964976806 1104 44-45 -0.2705326016256677 1104 46-47 -0.4859316752426821 1104 48-49 -0.44546316677521247 1104 50-51 -0.31365365740534656 1104 52-53 -0.5774355693519553 1104 54-55 -0.7026314378856213 1104 56-57 -3.4118612250053033 1104 58-59 -10.534636196550242 1104 60-61 -10.923812782869462 1104 62-63 -5.0080606139283255 1104 64-65 -6.0304737253675675 1104 66-67 -6.940121436874126 1104 68-69 -4.518614533857736 1104 70-71 -4.407690945169639 1104 72-73 -4.713346307000279 1104 74-75 -5.568162038653625 1104 76-77 -6.694802309719083 1104 78-79 -9.588063310977144 1104 80-81 -5.883224748262901 1104 82-83 -3.5487464746413515 1104 84-85 -2.995317279041025 1104 86-87 -2.882645120329901 1104 88-89 -2.948842484613582 1104 90-91 -5.229639030179754 1104 92-93 -7.262172343921019 1104 94-95 -7.78951300158922 1104 96-97 -7.796195136265872 1104 98-99 -8.078486642901073 1104 100-101 -8.122976475951933 1105 1 0.28608927785974814 1105 2 0.3350346456812474 1105 3 0.3485613165993584 1105 4 0.25618542711274017 1105 5 0.22856768911634617 1105 6 0.28004981853180055 1105 7 0.252999762625123 1105 8 0.2042923250260884 1105 9 0.3829709222178437 1105 10-11 0.43140700229638185 1105 12-13 0.31221998274197205 1105 14-15 0.4587147118833954 1105 16-17 0.47091488343841803 1105 18-19 0.4387010392371238 1105 20-21 0.47227280085727585 1105 22-23 0.6040012699921462 1105 24-25 0.4697871358696375 1105 26-27 0.5110341184319083 1105 28-29 0.5907495066171933 1105 30-31 0.6782055753015257 1105 32-33 0.5535746422618288 1105 34-35 0.6143191442586655 1105 36-37 0.3585580505450423 1105 38-39 0.481874426821598 1105 40-41 0.44760441542172913 1105 42-43 0.46586288282152566 1105 44-45 0.4502649109976673 1105 46-47 0.502962644844132 1105 48-49 0.48178593707713446 1105 50-51 0.5963208775553284 1105 52-53 0.5930474937092924 1105 54-55 0.4533595653630229 1105 56-57 1.6823317045828503 1105 58-59 -8.404471379290456 1105 60-61 -9.381175639522791 1105 62-63 -3.322663033443014 1105 64-65 -4.434892480805768 1105 66-67 -5.963571613752002 1105 68-69 -3.4943664748110947 1105 70-71 -3.564792264446808 1105 72-73 -2.1181791212902645 1105 74-75 -4.215503999192892 1105 76-77 -5.839369320062996 1105 78-79 -8.66022293444395 1105 80-81 -4.243661718931076 1105 82-83 -1.850845660633567 1105 84-85 -1.3086708029926086 1105 86-87 -1.079999175083099 1105 88-89 -1.3161630787082466 1105 90-91 -4.273295782950502 1105 92-93 -7.031193854824288 1105 94-95 -8.059368509327838 1105 96-97 -7.868721785530308 1105 98-99 -8.061532066752877 1105 100-101 -8.182844514639978 1106 1 0.20159313266072587 1106 2 0.18548748239606638 1106 3 0.10809455344888974 1106 4 0.13251776830131234 1106 5 0.04621512273821793 1106 6 0.21031296444989778 1106 7 0.15025998672240348 1106 8 0.13647513452519178 1106 9 0.10677511471213563 1106 10-11 0.13112710824651685 1106 12-13 0.15387140314737735 1106 14-15 0.28764073793402645 1106 16-17 0.20679047394659733 1106 18-19 0.21733988944146887 1106 20-21 0.23199263374005596 1106 22-23 0.2571137828490677 1106 24-25 0.43114036671168776 1106 26-27 0.3467458685206566 1106 28-29 0.4357842316838685 1106 30-31 0.37406587403722114 1106 32-33 0.34298190403330153 1106 34-35 0.4281208791466611 1106 36-37 0.3043068467730734 1106 38-39 0.2794309290106938 1106 40-41 0.21376545234953426 1106 42-43 0.15217128510436595 1106 44-45 0.33457628061255207 1106 46-47 0.2997900571667458 1106 48-49 0.3136406106250931 1106 50-51 0.4832250218077192 1106 52-53 0.5726493614900718 1106 54-55 0.4115497658854892 1106 56-57 1.7582471629264305 1106 58-59 -8.318288132883566 1106 60-61 -10.055824520401703 1106 62-63 -3.858441229924086 1106 64-65 -5.24717049979305 1106 66-67 -6.471847375382396 1106 68-69 -3.922471023203183 1106 70-71 -4.006428666147109 1106 72-73 -4.419900811031123 1106 74-75 -5.1429627432650555 1106 76-77 -6.372925203299392 1106 78-79 -8.782206677833447 1106 80-81 -4.552500454447127 1106 82-83 -2.284948133713673 1106 84-85 -1.8289296708553096 1106 86-87 -1.6291490253511576 1106 88-89 -1.5444319226320395 1106 90-91 -4.156375618745994 1106 92-93 -6.491830283831323 1106 94-95 -7.379587327310233 1106 96-97 -7.067352352691227 1106 98-99 -6.637126670941731 1106 100-101 -7.040083075392917 1107 1 0.13138672410455143 1107 2 0.05522107771346896 1107 3 -0.030974929990080113 1107 4 0.043718178723310075 1107 5 0.005483696329577015 1107 6 -0.0011741204154915863 1107 7 0.15223699067139052 1107 8 0.10792508033943449 1107 9 0.16570940727383032 1107 10-11 0.09566700241796866 1107 12-13 0.037420222814517956 1107 14-15 0.13853424850579188 1107 16-17 -0.02444890882365769 1107 18-19 0.1454082595959889 1107 20-21 0.23978872970428 1107 22-23 0.18902364968369056 1107 24-25 0.1781903107517735 1107 26-27 0.014634930389995304 1107 28-29 0.12254516530520476 1107 30-31 0.15986329174953795 1107 32-33 0.04341065900279517 1107 34-35 0.12453604406208285 1107 36-37 0.13747355705447006 1107 38-39 0.03896695699508257 1107 40-41 -0.046564955100741656 1107 42-43 -0.15222197486750844 1107 44-45 -0.157575137901091 1107 46-47 -0.1256153010875991 1107 48-49 -0.34817994211398684 1107 50-51 -0.16674577850039185 1107 52-53 -0.16494594408654706 1107 54-55 -0.2893006474195161 1107 56-57 1.006084241951882 1107 58-59 -8.656992583212304 1107 60-61 -10.109438407331627 1107 62-63 -4.184509679915731 1107 64-65 -5.675291651162777 1107 66-67 -6.94037040322042 1107 68-69 -3.824637418571447 1107 70-71 -3.9349914351354087 1107 72-73 -2.291243715949534 1107 74-75 -4.446357743390724 1107 76-77 -6.468523959614286 1107 78-79 -7.291458175149291 1107 80-81 -2.502775522937039 1107 82-83 -1.443486641084565 1107 84-85 -1.2027111566243427 1107 86-87 -1.1480452917824522 1107 88-89 -0.8924032463169063 1107 90-91 -0.433762785961914 1107 92-93 -3.37091088533019 1107 94-95 -6.04012457207166 1107 96-97 -6.1758412575475425 1107 98-99 -5.761424658602458 1107 100-101 -6.606071865668163 1108 1 -0.1455663722323166 1108 2 -0.19581991759433492 1108 3 -0.1754427475462954 1108 4 -0.16146180439189095 1108 5 -0.047484348909186735 1108 6 -0.31061389950912144 1108 7 -0.19940472098211615 1108 8 -0.0665213358525989 1108 9 -0.22258270346650733 1108 10-11 -0.19247075042025585 1108 12-13 -0.1612688873374779 1108 14-15 -0.2758021489391922 1108 16-17 -0.22614888548586976 1108 18-19 -0.1617895285853379 1108 20-21 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11.057847145624242 2316 96-97 12.62668183534296 2316 98-99 12.398651106836049 2316 100-101 11.268780884721044 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 37.0 5 119.0 6 243.0 7 582.0 8 1290.0 9 2174.0 10 3594.0 11 5857.0 12 8511.0 13 11771.0 14 14427.0 15 19320.0 16 26175.0 17 35247.0 18 36521.0 19 33325.0 20 33411.0 21 33633.0 22 32991.0 23 28666.0 24 28528.0 25 33116.0 26 46087.0 27 68114.0 28 95607.0 29 129276.0 30 159093.0 31 172792.0 32 172395.0 33 157656.0 34 140929.0 35 125620.0 36 100872.0 37 62183.0 38 22323.0 39 1453.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.236659076189376 19.081136380155982 14.537861438169433 21.144343105485213 2 15.4108487611217 22.930082085686934 37.99094806663395 23.668121086557417 3 20.890077398977848 26.579054118545386 30.831984780514894 21.698883701961876 4 12.32769364094766 16.88952110519026 38.52888954930546 32.253895704556626 5 12.27748787778724 39.796159995828916 33.74195111142008 14.184401014963763 6 29.241998444646665 37.33726546323824 17.44398179130282 15.976754300812276 7 26.36345592135162 34.93145707539342 21.120700407927746 17.584386595327214 8 25.838439724696855 32.23352717645074 20.288123579461487 21.63990951939091 9 26.279017806301024 17.28871761443073 21.536979772814288 34.89528480645396 10-11 24.842891959343888 26.039098589227276 27.24669378954139 21.871315661887444 12-13 27.831551112446405 24.31549268582622 27.13793294192805 20.715023259799324 14-15 23.608382582428682 25.20184333296727 28.395272629162786 22.794501455441264 16-17 20.757594820695093 28.592605889300927 28.491247612467557 22.158551677536426 18-19 20.42782047896067 28.143942002797257 30.756647004177445 20.671590514064636 20-21 22.591317353903623 25.616908013974317 29.821405523131272 21.970369108990788 22-23 21.50616503285342 26.053798697143304 30.03436105573476 22.405675214268513 24-25 22.547137195779175 25.988684632603064 30.26577419971501 21.198403971902746 26-27 22.202286127994427 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29.872650956882335 21.506764603524577 52-53 21.988991811710044 27.98503472627445 28.1992818622728 21.82669159974271 54-55 20.812454663945353 26.909718123377026 29.08880669941039 23.189020513267234 56-57 21.378130795560573 27.039969157597803 29.016614871861705 22.56528517497992 58-59 21.305157545372335 26.32841838158349 30.11686748746884 22.249556585575334 60-61 21.798536757414748 26.856405216038432 29.85124990528336 21.493808121263463 62-63 22.028459280450686 26.79318550054573 29.154343124826283 22.0240120941773 64-65 21.10840620692707 28.02925966075669 29.0588969583538 21.803437173962443 66-67 21.028503133570194 26.85196292453284 29.015030191397372 23.104503750499596 68-69 21.17225504707045 26.89096106113032 29.36331455018266 22.573469341616565 70-71 21.318618067918667 26.94724613043847 29.132142904948665 22.601992896694195 72-73 20.774525638266446 26.240930183608356 30.06759155290737 22.916952625217828 74-75 20.657614340584455 27.9393544324321 29.076023048790812 22.32700817819263 76-77 19.447745239773408 27.100793135054612 29.761580979058827 23.689880646113153 78-79 20.148674829785985 27.20936063472842 29.6205501702277 23.021414365257893 80-81 21.6596327915676 27.45368114570594 28.234506393428926 22.652179669297542 82-83 21.43579776138242 27.042564320463047 28.5904366738695 22.93120124428503 84-85 21.149764202105047 27.302988364122278 28.117488958497834 23.429758475274845 86-87 21.384514138560963 27.520104298036845 27.9752964633193 23.120085100082893 88-89 21.351531927078362 27.07863328329588 27.43048625994603 24.13934852967973 90-91 20.694367199507226 26.582334223303743 28.478210403792133 24.245088173396898 92-93 19.99726135767513 27.73136695547637 29.18028841769359 23.09108326915491 94-95 19.084586765701843 27.398489684081927 29.254320247174796 24.262603303041434 96-97 18.63998626321721 27.4517267669437 28.934876980219475 24.973409989619615 98-99 19.169751505156434 27.713990990374093 28.173712363526665 24.942545140942812 100-101 19.455539622536154 26.94446322405788 28.51290916710027 25.087087986305693 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2003.0 1 1459.0 2 963.5 3 1036.0 4 1182.5 5 1395.0 6 1750.0 7 2383.5 8 3136.5 9 3709.5 10 3840.5 11 3898.5 12 4211.5 13 4576.5 14 4868.5 15 5073.5 16 5262.5 17 5408.5 18 5565.5 19 5851.0 20 6154.0 21 6548.0 22 7181.0 23 8039.5 24 9013.5 25 10121.0 26 11668.5 27 13565.0 28 15567.0 29 17589.5 30 20029.5 31 22983.5 32 26255.0 33 29845.5 34 33299.5 35 36585.5 36 39758.5 37 42490.5 38 44626.0 39 46127.5 40 47836.0 41 50382.5 42 53283.0 43 57068.0 44 62384.5 45 69772.5 46 78908.5 47 89827.0 48 99341.0 49 95419.5 50 82317.5 51 72186.0 52 64568.5 53 58831.5 54 53962.5 55 49151.0 56 44362.0 57 39552.0 58 34783.0 59 29952.0 60 25018.0 61 20069.5 62 15465.5 63 11347.5 64 7909.0 65 5458.5 66 3714.5 67 2465.0 68 1604.0 69 985.5 70 619.5 71 446.5 72 339.5 73 243.0 74 149.5 75 97.5 76 56.0 77 24.5 78 12.0 79 5.0 80 4.5 81 4.0 82 2.5 83 2.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022560215710370216 2 0.008080461877031639 3 1.0846257553062604E-4 4 0.0 5 0.14674986469293702 6 0.0 7 0.0 8 0.0012473196186021994 9 0.0 10-11 0.0 12-13 0.0 14-15 6.236598093010997E-4 16-17 0.0 18-19 1.6269386329593907E-4 20-21 0.0 22-23 3.2538772659187814E-4 24-25 8.134693164796954E-5 26-27 3.525033704745346E-4 28-29 0.0037690744996892547 30-31 0.012798583912613872 32-33 0.0030098364709748725 34-35 0.0050977410499394235 36-37 0.11130971813830495 38-39 0.11068605832900387 40-41 0.12296944500784726 42-43 0.12494888701128119 44-45 0.12136962201877054 46-47 0.1149160987746983 48-49 0.11456359540422374 50-51 0.11318069756620827 52-53 0.09159664503561368 54-55 0.0793674896445356 56-57 0.5485494757461412 58-59 1.5606951149540607 60-61 0.5170953288422596 62-63 0.004962162830526142 64-65 0.12134250637488787 66-67 1.8978239153471292 68-69 2.8495016686967247 70-71 2.76503643800225 72-73 2.608172438141082 74-75 1.9973925596842437 76-77 1.39859779582354 78-79 0.9372793464479048 80-81 0.052495886556823 82-83 1.8980950717859557E-4 84-85 1.0846257553062604E-4 86-87 4.067346582398476E-4 88-89 1.0846257553062604E-4 90-91 0.24767429122418455 92-93 0.3946681967120655 94-95 1.987576696598722 96-97 2.40819456450649 98-99 1.3984893332480093 100-101 0.9332933467971544 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1843954.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.77758484236049 #Duplication Level Percentage of deduplicated Percentage of total 1 69.47420158575481 27.635159479326177 2 14.35150854266356 11.417366973433221 3 5.924737939356155 7.070152981544746 4 3.006022907326493 4.782893249370348 5 1.7823297993597493 3.544838740554989 6 1.1710361904311326 2.794859485100939 7 0.7968560839024635 2.2187907339206965 8 0.5679841029668932 1.8074428675902088 9 0.43467477128566023 1.5561281334284092 >10 2.2771984768265305 15.998127084319696 >50 0.13538954745918208 3.690342897895496 >100 0.06780728477051472 4.983679793182915 >500 0.003964403586800896 1.1633496531376348 >1k 0.004511217874635503 4.529581319842565 >5k 0.0012303321476278641 3.492387186723595 >10k+ 5.468142878346063E-4 3.31489942062837 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21898 1.1875567394848245 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15285 0.8289252334928094 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11930 0.6469792630401843 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11848 0.6425322974434287 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9362 0.5077133160588605 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8823 0.4784826519533568 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8216 0.4455642602798118 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7970 0.43222336348954477 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6862 0.3721350966455779 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6107 0.3311904743827666 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5990 0.324845413714225 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5599 0.30364098019798763 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5296 0.28720890000509774 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4581 0.2484335292528989 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4535 0.24593889001569452 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4424 0.23991921707374478 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4307 0.23357415640520318 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3391 0.18389829681217645 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3316 0.17983095022977796 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3314 0.17972248765424734 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3140 0.17028624358308286 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3113 0.16882199881341942 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2983 0.16177193140392873 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2912 0.15792150997259152 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2859 0.1550472517210299 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2848 0.1544507075556115 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2717 0.14734640885835545 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2709 0.14691255855623297 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2507 0.13595783842763973 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2454 0.13308358017607816 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2412 0.130805866089935 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2141 0.11610918710553517 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2137 0.11589226195447393 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2125 0.11524148650129017 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2006 0.1087879632572179 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1895 0.10276829031526816 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 5.423128776531302E-5 0.0 9 0.0 0.0 0.0 5.423128776531302E-5 0.0 10-11 0.0 0.0 0.0 5.423128776531302E-5 0.0 12-13 0.0 0.0 0.0 1.3557821941328254E-4 0.0 14-15 0.0 0.0 0.0 2.711564388265651E-4 0.0 16-17 0.0 0.0 0.0 3.796190143571911E-4 0.0 18-19 0.0 0.0 0.0 6.236598093010997E-4 0.0 20-21 0.0 0.0 0.0 0.00116597268695423 0.0 22-23 0.0 0.0 0.0 0.0024675235933217425 0.0 24-25 0.0 0.0 0.0 0.004013115294633164 0.0 26-27 0.0 0.0 0.0 0.0057756321470058365 0.0 28-29 0.0 0.0 0.0 0.011090298348006513 0.0 30-31 0.0 0.0 0.0 0.024946392372043987 0.0 32-33 0.0 0.0 0.0 0.051817995459756586 0.0 34-35 0.0 0.0 0.0 0.08994259075877165 0.0 36-37 0.0 0.0 0.0 0.14455349753844185 0.0 38-39 0.0 0.0 0.0 0.22549369452817153 0.0 40-41 0.0 0.0 0.0 0.3129145304058561 0.0 42-43 0.0 0.0 0.0 0.40179961105320416 0.0 44-45 0.0 0.0 0.0 0.5051915611777734 0.0 46-47 0.0 0.0 0.0 0.6258561764555949 0.0 48-49 0.0 0.0 0.0 0.7338849016840984 0.0 50-51 0.0 0.0 0.0 0.8383885932078565 0.0 52-53 0.0 0.0 0.0 0.9360591424731853 0.0 54-55 0.0 0.0 0.0 1.043138820165796 0.0 56-57 0.0 0.0 0.0 1.1386130022766294 0.0 58-59 0.0 0.0 0.0 1.2039888196777144 0.0 60-61 0.0 0.0 2.711564388265651E-5 1.2600097399392827 0.0 62-63 0.0 0.0 5.423128776531302E-5 1.3119904292623352 0.0 64-65 2.711564388265651E-5 0.0 5.423128776531302E-5 1.3585479898088564 0.0 66-67 5.423128776531302E-5 0.0 1.0846257553062604E-4 1.3969166259028154 0.0 68-69 5.423128776531302E-5 0.0 1.0846257553062604E-4 1.4510394510925977 0.0 70-71 5.423128776531302E-5 0.0 1.0846257553062604E-4 1.5368062326934404 0.0 72-73 5.423128776531302E-5 0.0 1.0846257553062604E-4 1.654298317636991 0.0 74-75 5.423128776531302E-5 0.0 1.0846257553062604E-4 1.8080169028077706 0.0 76-77 5.423128776531302E-5 0.0 1.0846257553062604E-4 2.0328598218827585 0.0 78-79 5.423128776531302E-5 0.0 1.0846257553062604E-4 2.417251189563297 0.0 80-81 5.423128776531302E-5 0.0 1.0846257553062604E-4 2.8718720748999162 0.0 82-83 5.423128776531302E-5 5.423128776531302E-5 1.0846257553062604E-4 3.291513779627908 0.0 84-85 5.423128776531302E-5 5.423128776531302E-5 1.0846257553062604E-4 3.641793667304065 0.0 86-87 5.423128776531302E-5 5.423128776531302E-5 1.0846257553062604E-4 3.904245984444297 0.0 88-89 5.423128776531302E-5 5.423128776531302E-5 1.6269386329593904E-4 4.143243269626032 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11360 0.0 25.705324 1 TACATGG 11395 0.0 25.49683 2 ACATGGG 11620 0.0 24.028603 3 TTTGCGT 675 0.0 23.019196 3 GAGTACT 10405 0.0 22.99892 12-13 CATGGGA 8145 0.0 22.313938 4 AGTACTT 10635 0.0 21.197414 12-13 TACGCGG 150 1.2187229E-10 21.066755 64-65 CTTTGCG 740 0.0 20.998951 2 GTACTTT 11435 0.0 20.947298 14-15 ATGGGAG 2845 0.0 20.852987 5 GCTTACG 730 0.0 20.71168 60-61 TAGGACG 160 5.340566E-5 20.599566 4 CATGGGG 3400 0.0 20.218733 4 TTACGCG 205 0.0 20.130306 62-63 ACTTTTT 12670 0.0 19.220875 16-17 TACTTTT 12300 0.0 18.231956 14-15 ATGGGAT 2945 0.0 18.066462 5 ATGGGGA 1835 0.0 17.70488 5 ATACCGC 560 0.0 16.472174 62-63 >>END_MODULE