FastQCFastQC Report
Fri 27 May 2016
SRR939580_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939580_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817996
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT81930.4506610575600826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59770.32876860015093545No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45560.25060561189353553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42720.23498401536637042No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33790.1858639953003197No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA32990.1814635455743577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31960.17579796655218163No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30450.16749211769442837No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25550.14053936312291115No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23900.13146343556311454No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT21450.11798705827735594No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21210.11666692335956735No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG93200.037.089911
TACATGG113750.029.7387452
ACATGGG112700.028.5074233
ATGGGAG28350.027.6373985
CATGGGA79300.026.1519724
CATGGGG33700.024.250984
GAGTACT94600.023.77496112-13
GTATAGT3902.13171E-723.5315131
ATGGGAT30100.022.4239275
AGTACTT100550.022.36809212-13
GTACTTT105000.021.58071514-15
CCTTTAG6552.0918378E-1020.2382981
GTATCGC1405.064095E-420.2291346
GTATAGG3556.429204E-520.1067391
TACTTTT116550.019.17880814-15
ACTTTTT125200.019.17636716-17
TTTAGGC4500.018.8987453
ATGGGGA18900.018.7301645
TACCTGG10600.018.7199362
TTTGCGT4900.018.3202113
Produced by FastQC (version 0.11.5)