##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939580_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1817996 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.069421494876776 2.0 2.0 31.0 2.0 33.0 2 22.416468463076928 16.0 16.0 31.0 16.0 34.0 3 26.882587200411884 28.0 25.0 31.0 16.0 34.0 4 32.1244408678567 35.0 32.0 35.0 25.0 37.0 5 32.78642912305638 35.0 33.0 35.0 26.0 37.0 6 33.2101814305422 35.0 33.0 37.0 28.0 37.0 7 33.386009650186246 35.0 33.0 37.0 28.0 37.0 8 33.32450676459134 35.0 33.0 37.0 26.0 37.0 9 34.62732646276449 37.0 34.0 39.0 27.0 39.0 10-11 34.46867429851331 37.0 33.0 39.0 27.0 39.0 12-13 34.48852802756441 37.0 33.0 39.0 27.0 39.0 14-15 35.02921156042147 38.0 33.0 40.0 26.0 41.0 16-17 34.69716380014037 38.0 33.0 40.0 25.0 41.0 18-19 35.06726032400512 38.0 33.5 40.0 26.0 41.0 20-21 35.07628647147739 38.0 34.0 40.0 25.5 41.0 22-23 34.89127093788986 38.0 33.5 40.0 25.0 41.0 24-25 34.644581726252426 38.0 33.0 40.0 24.0 41.0 26-27 34.66928722615451 38.0 33.0 40.0 24.5 41.0 28-29 34.39744394377105 38.0 33.0 40.0 24.0 41.0 30-31 34.246887781931314 38.0 33.0 40.0 24.0 41.0 32-33 33.87320049109019 38.0 33.0 40.0 22.0 41.0 34-35 33.53514034134288 37.0 32.0 40.0 19.0 41.0 36-37 33.175215181991604 37.0 31.0 40.0 17.5 41.0 38-39 32.91242692503174 37.0 31.0 40.0 16.0 41.0 40-41 32.527959357446335 37.0 30.5 40.0 15.0 41.0 42-43 32.21468061535889 37.0 30.0 39.5 14.5 41.0 44-45 31.77555341155866 36.0 30.0 39.0 9.0 41.0 46-47 31.314242990633645 36.0 29.5 39.0 8.0 40.0 48-49 30.829339833530987 36.0 28.5 39.0 4.5 40.0 50-51 30.110444137390843 34.5 27.5 38.5 2.0 40.0 52-53 30.14057924219855 35.0 28.0 38.5 2.0 40.0 54-55 30.263047333437477 35.0 28.0 39.0 2.0 40.0 56-57 29.944596412753384 35.0 27.5 39.0 2.0 40.0 58-59 29.522286077637133 35.0 26.5 38.0 2.0 40.0 60-61 28.857282139234627 34.0 26.0 38.0 2.0 40.0 62-63 28.47562425879925 34.0 26.0 38.0 2.0 40.0 64-65 27.878774210724337 33.5 24.0 37.0 2.0 39.0 66-67 27.2303946213303 33.0 22.5 36.5 2.0 39.0 68-69 26.89332072237783 32.5 22.0 36.0 2.0 39.0 70-71 26.323918204440496 32.0 21.0 35.5 2.0 38.5 72-73 25.711256515415876 31.5 19.5 35.0 2.0 38.0 74-75 25.172182722074197 31.0 18.0 35.0 2.0 37.0 76-77 24.54557435769936 31.0 13.5 34.5 2.0 36.5 78-79 23.87461606076141 30.5 7.5 34.0 2.0 36.0 80-81 23.115251078660236 29.5 4.5 34.0 2.0 35.5 82-83 22.41056911016306 29.0 2.0 34.0 2.0 35.0 84-85 21.725288724507642 29.0 2.0 34.0 2.0 35.0 86-87 21.07322953405838 28.5 2.0 33.0 2.0 35.0 88-89 20.239199096147626 26.5 2.0 33.0 2.0 34.5 90-91 19.277174427226463 25.5 2.0 32.0 2.0 34.0 92-93 18.31818716872864 24.5 2.0 32.0 2.0 34.0 94-95 17.357836595900103 21.5 2.0 31.5 2.0 34.0 96-97 16.359647105934226 17.0 2.0 31.0 2.0 34.0 98-99 15.339180614258778 2.0 2.0 31.0 2.0 34.0 100-101 13.807263877368268 2.0 2.0 29.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.770687136164575 1101 2 7.924062651876277 1101 3 3.7913249101460984 1101 4 2.3854014613323855 1101 5 1.6493314932710277 1101 6 1.2612139299253968 1101 7 1.011199838630013 1101 8 0.9549475883692011 1101 9 1.1531999468731087 1101 10-11 1.0530938694846768 1101 12-13 0.8950057393409878 1101 14-15 0.9939647586267668 1101 16-17 1.0890612258998829 1101 18-19 1.1104285568161245 1101 20-21 0.9075432955999432 1101 22-23 0.912384436480032 1101 24-25 1.1718322912822785 1101 26-27 1.0894495462489573 1101 28-29 1.0999029919103123 1101 30-31 1.0367051764023643 1101 32-33 1.0538654052333172 1101 34-35 1.032800951590012 1101 36-37 0.9011854486139512 1101 38-39 0.920465115313398 1101 40-41 1.007420779777469 1101 42-43 0.7793848992825332 1101 44-45 0.544867589294828 1101 46-47 0.3750490175312926 1101 48-49 0.6288417670835607 1101 50-51 0.5709076055102109 1101 52-53 0.5995851222006046 1101 54-55 0.5813850116878392 1101 56-57 0.2475848369464515 1101 58-59 0.1547521392545228 1101 60-61 0.1264249725581017 1101 62-63 0.22077560329186596 1101 64-65 0.25290362754386564 1101 66-67 0.462847139419317 1101 68-69 0.16188108984859184 1101 70-71 -0.07814988418850888 1101 72-73 0.12527448189685586 1101 74-75 -0.12205253419431017 1101 76-77 -0.1831289515225798 1101 78-79 -0.2930757096304326 1101 80-81 -0.4647720011450964 1101 82-83 -0.3784660059206644 1101 84-85 -0.46178076153416114 1101 86-87 -0.4659463352530082 1101 88-89 -0.20770812639469227 1101 90-91 -0.11274449559142852 1101 92-93 -0.3740382045536528 1101 94-95 -0.6292674456092904 1101 96-97 -1.3146121630842185 1101 98-99 -1.3796038012212772 1101 100-101 -1.3045883585267113 1102 1 14.795264325740106 1102 2 7.929605940682389 1102 3 3.763761808659236 1102 4 2.2059506572111474 1102 5 1.3707575377221133 1102 6 1.146061920667151 1102 7 1.0291679940045668 1102 8 0.8227289366180628 1102 9 0.8552565617643424 1102 10-11 0.938710044562427 1102 12-13 0.638150797855431 1102 14-15 0.7245863522001557 1102 16-17 0.8150132299780282 1102 18-19 0.8679573519557735 1102 20-21 0.8113383616785157 1102 22-23 0.9586716632521686 1102 24-25 0.9809796890799092 1102 26-27 0.956714503916416 1102 28-29 0.6850576971718141 1102 30-31 0.9108303733783671 1102 32-33 0.7811320052258992 1102 34-35 1.1570107665723768 1102 36-37 1.0386863242263686 1102 38-39 0.8310670009852075 1102 40-41 0.9614674630165112 1102 42-43 0.8236114200667686 1102 44-45 0.6872679210562787 1102 46-47 0.8774306832963497 1102 48-49 0.7245554750828624 1102 50-51 0.7067976486050576 1102 52-53 0.9632603632956993 1102 54-55 0.9934735253600486 1102 56-57 0.9780948819132007 1102 58-59 0.8546279641697154 1102 60-61 1.1272274455818838 1102 62-63 0.6011214746100997 1102 64-65 0.5283052172439575 1102 66-67 0.44705668362405504 1102 68-69 0.24646274725485995 1102 70-71 0.3540538747935038 1102 72-73 0.5203715527305128 1102 74-75 0.2707496963069005 1102 76-77 0.09351762658410934 1102 78-79 0.0722932160475871 1102 80-81 -0.05481159354237164 1102 82-83 -0.19091674352478805 1102 84-85 -0.5141523908678529 1102 86-87 -0.7677409433642133 1102 88-89 -0.47046425406280434 1102 90-91 -0.4061932665259249 1102 92-93 -0.2341406526776666 1102 94-95 -0.25503320177639566 1102 96-97 -0.7878727259131608 1102 98-99 -0.6404621309589569 1102 100-101 -0.5977547260625933 1103 1 14.858497564924644 1103 2 7.915148458379502 1103 3 3.9025453860103028 1103 4 2.1938564388005375 1103 5 1.644673830067319 1103 6 1.2816044664348638 1103 7 1.02473227764564 1103 8 1.1214733947796773 1103 9 1.087415308872373 1103 10-11 1.1282538299278713 1103 12-13 0.7945106416257062 1103 14-15 1.1434102445482779 1103 16-17 1.1555774097578748 1103 18-19 1.0964506399943943 1103 20-21 0.873900011735806 1103 22-23 1.0610392500169965 1103 24-25 1.1600107536911395 1103 26-27 1.0633002598829577 1103 28-29 1.0753573676790964 1103 30-31 1.0681512141714649 1103 32-33 1.0166832248093058 1103 34-35 1.004305153631151 1103 36-37 1.2172483290960514 1103 38-39 1.3513589576913532 1103 40-41 1.1521283179459587 1103 42-43 0.9299230666152454 1103 44-45 0.8077754319069719 1103 46-47 0.6707106375749525 1103 48-49 0.6021296193646286 1103 50-51 0.42142125272425446 1103 52-53 0.6446305510824075 1103 54-55 0.7180794562288959 1103 56-57 0.8436842831279847 1103 58-59 0.6484192244442575 1103 60-61 0.7451516190290022 1103 62-63 0.5668486744048487 1103 64-65 0.4005741583029909 1103 66-67 0.44919383882584896 1103 68-69 0.42250850345719826 1103 70-71 0.3245533574523307 1103 72-73 0.6213266268565505 1103 74-75 0.5456053713122202 1103 76-77 0.5710548299550524 1103 78-79 0.2581020676063197 1103 80-81 0.39177342136031257 1103 82-83 0.23126790151965793 1103 84-85 -0.157877657159613 1103 86-87 -0.048660289229385256 1103 88-89 0.02075329702428519 1103 90-91 -0.018752446370335463 1103 92-93 0.16240402477148308 1103 94-95 0.04137171215071689 1103 96-97 -0.6258518840533824 1103 98-99 -0.8016547497383026 1103 100-101 -0.6239698602709431 1104 1 14.633876399117945 1104 2 7.909559889838839 1104 3 3.6723943414142433 1104 4 2.1738701330758445 1104 5 1.485349088261465 1104 6 1.0466539043989798 1104 7 0.8208978805605653 1104 8 0.7924187073433586 1104 9 0.941420476831027 1104 10-11 0.8730548961250264 1104 12-13 0.7078572616666179 1104 14-15 0.767104193797941 1104 16-17 1.0253436289151878 1104 18-19 0.7212201604502084 1104 20-21 0.781097250056952 1104 22-23 0.7714061424761738 1104 24-25 0.94923344054056 1104 26-27 0.7802379590816741 1104 28-29 0.9606559925456253 1104 30-31 0.9701621532889959 1104 32-33 0.8196001869625675 1104 34-35 1.0789450675528514 1104 36-37 1.1552014425058488 1104 38-39 1.127064661193991 1104 40-41 1.2367858387930468 1104 42-43 1.1893370657512037 1104 44-45 0.8562731373007217 1104 46-47 0.7241337988331971 1104 48-49 0.6765567829378583 1104 50-51 0.6474868385213348 1104 52-53 0.7475140926828452 1104 54-55 0.8683631233601901 1104 56-57 0.7251101995439946 1104 58-59 0.5642161416020421 1104 60-61 0.49729053440979243 1104 62-63 0.3072769465622649 1104 64-65 0.48798520664867695 1104 66-67 0.2788578399996453 1104 68-69 0.38273682289247546 1104 70-71 0.2347930887836931 1104 72-73 0.48146976463889146 1104 74-75 0.3356179243979156 1104 76-77 0.24633054951585365 1104 78-79 0.07651353167107189 1104 80-81 0.006813485266931707 1104 82-83 -0.16849489406883222 1104 84-85 0.08516760731108164 1104 86-87 0.22994892669184352 1104 88-89 0.12083644083862666 1104 90-91 -0.0055415463793018205 1104 92-93 0.1380706017732507 1104 94-95 0.37849175714619676 1104 96-97 -0.2881107058566261 1104 98-99 -0.7778047020528778 1104 100-101 -0.7339552693466622 1105 1 14.90784932414779 1105 2 7.984498498607255 1105 3 4.043673003466374 1105 4 2.396307040802519 1105 5 1.8484378859825412 1105 6 1.4107410030834657 1105 7 1.0379946534604585 1105 8 1.144449998490856 1105 9 1.2830524876963025 1105 10-11 1.0088036418991493 1105 12-13 0.929841137528669 1105 14-15 0.9853152994282866 1105 16-17 1.263149666042679 1105 18-19 0.9727819774435247 1105 20-21 0.9285054506154538 1105 22-23 0.9097962150762342 1105 24-25 1.080851696027004 1105 26-27 1.1434631074967356 1105 28-29 1.2503661938500414 1105 30-31 1.1417552240001996 1105 32-33 0.8250980608274787 1105 34-35 1.0990828619600634 1105 36-37 1.004854617137667 1105 38-39 1.0092734354948263 1105 40-41 1.4200848839371574 1105 42-43 1.262848002547301 1105 44-45 1.0722745596256082 1105 46-47 1.0372877641653666 1105 48-49 1.1182219457365754 1105 50-51 1.011547201775624 1105 52-53 1.2565772698411628 1105 54-55 1.174361350801167 1105 56-57 1.1470490262061688 1105 58-59 1.1885997896782285 1105 60-61 1.2507803769514787 1105 62-63 1.0854818228373802 1105 64-65 1.0353129335622988 1105 66-67 1.0878132733742838 1105 68-69 1.0331591758509475 1105 70-71 0.8257327188455434 1105 72-73 0.810790109900239 1105 74-75 0.7710267291771444 1105 76-77 0.805945078083333 1105 78-79 0.714684106389246 1105 80-81 0.729475505315829 1105 82-83 0.5715966213087214 1105 84-85 0.5242473203329716 1105 86-87 0.686582053761331 1105 88-89 0.6710924994213023 1105 90-91 0.869307498697971 1105 92-93 0.9632687447302715 1105 94-95 0.9752810993342997 1105 96-97 0.3118082857898905 1105 98-99 0.2828605289211854 1105 100-101 0.3078918280545224 1106 1 14.844193688361301 1106 2 8.001476904451227 1106 3 3.6930504752382944 1106 4 2.172635890426612 1106 5 1.7052372133995135 1106 6 1.1950930052486868 1106 7 0.9258061107076969 1106 8 0.9084497576737434 1106 9 1.1284383092156602 1106 10-11 0.9486686244798648 1106 12-13 0.7173413439813956 1106 14-15 0.9955762663303247 1106 16-17 1.1124307843291703 1106 18-19 1.1191648191174508 1106 20-21 0.9858911836931199 1106 22-23 0.9108098313661301 1106 24-25 1.0506941472895122 1106 26-27 0.8189095648478641 1106 28-29 1.0739235687942994 1106 30-31 1.090958795978679 1106 32-33 1.0800554569764245 1106 34-35 1.3044897389936239 1106 36-37 1.353667687113692 1106 38-39 1.2863934652453324 1106 40-41 1.2942731251357387 1106 42-43 0.9865367347037903 1106 44-45 1.1369849690519978 1106 46-47 1.2984679118842841 1106 48-49 1.1848803365348068 1106 50-51 1.0807770611799832 1106 52-53 1.0322376122170347 1106 54-55 0.8494491131892623 1106 56-57 0.8764350544446415 1106 58-59 0.9272349000678339 1106 60-61 1.0382351638043552 1106 62-63 0.9922949226491475 1106 64-65 1.0634508418525321 1106 66-67 0.8562327192498387 1106 68-69 0.8190486514503661 1106 70-71 0.8400958348272205 1106 72-73 0.7520584382405247 1106 74-75 0.7516795779850156 1106 76-77 0.61369816381902 1106 78-79 0.798041326081055 1106 80-81 0.9790483479647527 1106 82-83 0.6381018375033491 1106 84-85 0.49037162458728645 1106 86-87 0.6182849610952239 1106 88-89 0.7226606610543413 1106 90-91 1.0568728642951157 1106 92-93 1.3034269168218025 1106 94-95 1.1670714200845858 1106 96-97 0.7037781005365282 1106 98-99 0.7287964204213555 1106 100-101 0.736118604253905 1107 1 14.718076615838793 1107 2 7.925446934516675 1107 3 3.7589868228679393 1107 4 2.3105746216980023 1107 5 1.565066413426095 1107 6 1.0279448000395632 1107 7 0.8396088082924962 1107 8 0.7295790390929611 1107 9 0.516682781990653 1107 10-11 0.7550574280079942 1107 12-13 0.6150783377689777 1107 14-15 0.5271089085182936 1107 16-17 1.0028450355168346 1107 18-19 0.8344558912579174 1107 20-21 0.7165653618765759 1107 22-23 0.7421993616142899 1107 24-25 0.7747157225694039 1107 26-27 0.7984848385098715 1107 28-29 0.9726315124672666 1107 30-31 0.738866568976249 1107 32-33 0.8496550965232856 1107 34-35 0.8808877407211426 1107 36-37 0.7839565479488755 1107 38-39 0.7623548639530142 1107 40-41 0.9250329005584845 1107 42-43 0.9578009265080567 1107 44-45 0.7988117667497541 1107 46-47 0.8894553375512082 1107 48-49 0.9596466960411334 1107 50-51 0.9714018016371107 1107 52-53 0.8538807885234725 1107 54-55 0.8282335597192798 1107 56-57 0.790941891842067 1107 58-59 0.9635997114528401 1107 60-61 1.1687125565820011 1107 62-63 1.0281108008591744 1107 64-65 0.9476020639880112 1107 66-67 0.7681895479266245 1107 68-69 0.8846133336868398 1107 70-71 0.6700176501323618 1107 72-73 0.6542935695484537 1107 74-75 0.6211230246313093 1107 76-77 0.5028806245346118 1107 78-79 0.4593002439741447 1107 80-81 0.27902151123175756 1107 82-83 0.5063417838506474 1107 84-85 0.4616007781037794 1107 86-87 0.41847717854647115 1107 88-89 0.36614815939326917 1107 90-91 0.5818886445017561 1107 92-93 0.6984807563952415 1107 94-95 0.7942941452432173 1107 96-97 0.13010322262549678 1107 98-99 -0.18973229332569552 1107 100-101 -0.1398603914605232 1108 1 14.497386016115936 1108 2 7.596279630046951 1108 3 3.3715275079892706 1108 4 0.20208532873289897 1108 5 0.5330530638724227 1108 6 0.7918376612106499 1108 7 0.6706930466347956 1108 8 0.7814485405592393 1108 9 0.7201159415730061 1108 10-11 0.8442197777550149 1108 12-13 0.5348168653704093 1108 14-15 0.4494871854832567 1108 16-17 0.7332612926468087 1108 18-19 0.5742365239316953 1108 20-21 0.6389934881857471 1108 22-23 -0.1059756488591006 1108 24-25 0.44873097143344154 1108 26-27 0.6239811667783783 1108 28-29 0.4967054727811444 1108 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2312 10-11 0.09119666178226282 2312 12-13 -0.5883597182378253 2312 14-15 0.02242551813382221 2312 16-17 -0.22234473202396998 2312 18-19 -0.07118963264554168 2312 20-21 -0.08029549621114285 2312 22-23 0.22051848051272316 2312 24-25 0.16944610761620282 2312 26-27 0.25574592480212743 2312 28-29 -0.4169154386814924 2312 30-31 0.0739005579141292 2312 32-33 0.4116604922067424 2312 34-35 -0.5191253133517293 2312 36-37 0.19838178195521294 2312 38-39 0.17204449128340826 2312 40-41 0.22226350317317412 2312 42-43 0.18945789931690626 2312 44-45 0.3429368296026958 2312 46-47 0.534447408410653 2312 48-49 0.653819623827939 2312 50-51 0.530627767638503 2312 52-53 0.46169437636287824 2312 54-55 0.4946974047722712 2312 56-57 0.6687162449402351 2312 58-59 0.4531756716524207 2312 60-61 0.3873673101984636 2312 62-63 0.29276762378792753 2312 64-65 0.4513726362398849 2312 66-67 0.2209134977989855 2312 68-69 0.399292880372073 2312 70-71 0.47125694095776893 2312 72-73 0.12477093765396674 2312 74-75 0.2970608630702358 2312 76-77 0.3834450439668089 2312 78-79 0.6874108603197833 2312 80-81 0.7219567195167436 2312 82-83 0.5404895462858619 2312 84-85 0.6652244920358612 2312 86-87 0.6762858106730292 2312 88-89 1.1738953890289139 2312 90-91 1.4511777293628931 2312 92-93 1.773753683215073 2312 94-95 2.1194822205893935 2312 96-97 1.8945004145493733 2312 98-99 1.5936860123523413 2312 100-101 1.4436068669907804 2313 1 -14.160955642225723 2313 2 -7.382154645509628 2313 3 -3.635099198637434 2313 4 -1.2792668921876675 2313 5 -0.5716285854135421 2313 6 -1.0883710988542248 2313 7 -0.5937806092057656 2313 8 -0.17196327544250067 2313 9 -2.4615055544369824 2313 10-11 -0.574510772270493 2313 12-13 -0.8312853777102447 2313 14-15 -1.0617471437366746 2313 16-17 -0.9177883771079038 2313 18-19 -0.09373159907239881 2313 20-21 -1.1280173187531162 2313 22-23 -2.1420714475448293 2313 24-25 -1.396740942743513 2313 26-27 -0.5663164253177797 2313 28-29 0.01186153973577575 2313 30-31 -1.2980418881290348 2313 32-33 -0.6279078531170015 2313 34-35 -1.676199653879305 2313 36-37 -1.524399992625117 2313 38-39 -0.9097300890523243 2313 40-41 -0.5583120363951721 2313 42-43 -0.35490660907638016 2313 44-45 -0.34507276272344356 2313 46-47 -1.0434902414694456 2313 48-49 -0.2073314553087542 2313 50-51 -1.0427535273255266 2313 52-53 -0.3152840409033111 2313 54-55 -1.0463097894723354 2313 56-57 -0.5732501819182758 2313 58-59 -0.5535389566449389 2313 60-61 -1.8018413228950614 2313 62-63 -0.5791748222552364 2313 64-65 -0.41865134457546205 2313 66-67 -1.4706932168293108 2313 68-69 -0.7112586783809292 2313 70-71 -0.3903737544858785 2313 72-73 -1.4246295419623358 2313 74-75 -0.6367520865700556 2313 76-77 -0.1590009991986605 2313 78-79 -0.05815268884089164 2313 80-81 -0.08283944355280326 2313 82-83 -0.12953443452948576 2313 84-85 -0.16235344561401988 2313 86-87 -0.35896598788812284 2313 88-89 -0.0637544910670087 2313 90-91 0.2825925974684118 2313 92-93 0.7337057215843785 2313 94-95 0.7940625563208066 2313 96-97 0.5964188797771932 2313 98-99 0.543566108275602 2313 100-101 0.3373718550003737 2314 1 -14.160955642225723 2314 2 -7.230312801911335 2314 3 -3.6591642392878434 2314 4 -1.737742648168247 2314 5 -1.3336324457399193 2314 6 -6.0937708765930125 2314 7 -1.9714258101182054 2314 8 -4.52045833305495 2314 9 -1.4922594878756001 2314 10-11 -0.43444935798729034 2314 12-13 -3.1332172955905833 2314 14-15 -3.1773440320796595 2314 16-17 -2.8205327182203774 2314 18-19 -1.3825442562637207 2314 20-21 -2.2689285897363263 2314 22-23 -1.521135610848333 2314 24-25 -3.095664145644605 2314 26-27 -4.750038715778093 2314 28-29 -2.2715706413486245 2314 30-31 -2.223321176893137 2314 32-33 -4.109704659574277 2314 34-35 -2.69740395872811 2314 36-37 -1.1282503757332663 2314 38-39 -1.694788871294822 2314 40-41 -2.577107146648437 2314 42-43 -1.6570321281732987 2314 44-45 -2.4364186128725933 2314 46-47 -2.1418566215361245 2314 48-49 -1.2428230620233833 2314 50-51 -2.1266103443109614 2314 52-53 -0.7625344357094157 2314 54-55 -0.16477735688182094 2314 56-57 -0.7943755255458136 2314 58-59 -1.2318480167139576 2314 60-61 -0.568691062615482 2314 62-63 -2.7531018269929106 2314 64-65 -1.041579928537562 2314 66-67 -1.9143733946382824 2314 68-69 -1.7572965797671358 2314 70-71 -2.12372521965521 2314 72-73 -1.9964000280125198 2314 74-75 -1.4652845776503582 2314 76-77 -0.4681844816809644 2314 78-79 0.003921359237718747 2314 80-81 0.15055647385960924 2314 82-83 0.12451890816221578 2314 84-85 0.29291765457899643 2314 86-87 0.4430560042742435 2314 88-89 0.6293314304396844 2314 90-91 1.2184661425347976 2314 92-93 1.7223581167414252 2314 94-95 1.6615925323399914 2314 96-97 1.8989643555916622 2314 98-99 1.7880300493178911 2314 100-101 1.9632641168006888 2315 1 -14.160955642225723 2315 2 -7.404990911492048 2315 3 -4.806975197359073 2315 4 -3.0319189220498757 2315 5 -3.544480141177562 2315 6 -2.866537520654468 2315 7 -3.322404459885526 2315 8 -3.7094866986981465 2315 9 -2.466435761757225 2315 10-11 -4.664876830334876 2315 12-13 -4.680162785850854 2315 14-15 -3.741311948268894 2315 16-17 -4.5277578895133175 2315 18-19 -1.6901216011646838 2315 20-21 -3.1747866913662435 2315 22-23 -4.168121556642596 2315 24-25 -4.960193996216361 2315 26-27 -4.674279297589472 2315 28-29 -2.2887406247454862 2315 30-31 -3.033936662242624 2315 32-33 -4.242417051127724 2315 34-35 -3.9223444446622118 2315 36-37 -5.23833105295423 2315 38-39 -3.568703236517095 2315 40-41 -4.890820871129883 2315 42-43 -3.3570658021437865 2315 44-45 -1.9738187017344586 2315 46-47 -1.340659792501679 2315 48-49 -1.2187099268364214 2315 50-51 -0.9373719840462478 2315 52-53 -0.7385566979454588 2315 54-55 -2.3154050022762043 2315 56-57 -4.251105175009137 2315 58-59 -3.483835716360847 2315 60-61 -4.302416011753753 2315 62-63 -2.5669497796622736 2315 64-65 -3.3057968918001954 2315 66-67 -1.9607967045089651 2315 68-69 -1.487763572260647 2315 70-71 -2.0701522710781646 2315 72-73 -2.3195873628580657 2315 74-75 -3.0068864297187368 2315 76-77 -2.630938731850506 2315 78-79 -1.954800432965822 2315 80-81 -1.702979994582737 2315 82-83 -1.1781142092523744 2315 84-85 -1.1319713850113011 2315 86-87 -1.8442234195267027 2315 88-89 -2.2251341122023156 2315 90-91 -1.8127236710891275 2315 92-93 -0.7469508653225105 2315 94-95 -0.5588676785900155 2315 96-97 -1.364370302874768 2315 98-99 -0.78059703484365 2315 100-101 -0.5571710724984733 2316 1 -14.160955642225723 2316 2 -7.300781298339865 2316 3 -4.921710822403018 2316 4 -2.548895113845404 2316 5 -2.048764584239546 2316 6 -0.9457610683744804 2316 7 -4.431253574010221 2316 8 -2.910127246451225 2316 9 -1.3248398311632457 2316 10-11 -4.435077297195285 2316 12-13 -3.3969895506516465 2316 14-15 -1.7099371686958236 2316 16-17 -3.190344727365236 2316 18-19 -2.4707067552272193 2316 20-21 -3.472075748551287 2316 22-23 -2.98235618915416 2316 24-25 -3.424210681377083 2316 26-27 -4.143550839825998 2316 28-29 -3.970188488041906 2316 30-31 -2.7183969299818997 2316 32-33 -2.803468385749664 2316 34-35 -4.255894227506467 2316 36-37 -2.384397570085248 2316 38-39 -3.392698702020134 2316 40-41 -2.967758777205539 2316 42-43 -6.809201900380977 2316 44-45 -3.648780995748485 2316 46-47 -4.74320477775688 2316 48-49 -4.601880857069521 2316 50-51 -3.5539110704508126 2316 52-53 -4.718336091734717 2316 54-55 -4.70043899703392 2316 56-57 -4.823544788389487 2316 58-59 -3.7890537405439026 2316 60-61 -3.4763586492962055 2316 62-63 -2.4060506892158706 2316 64-65 -2.756569800485817 2316 66-67 -2.982226603039237 2316 68-69 -3.006295383354008 2316 70-71 -3.102871264976759 2316 72-73 -4.023253935308272 2316 74-75 -4.404548961260684 2316 76-77 -4.1713784309104085 2316 78-79 -4.411437268559343 2316 80-81 -4.346830251877488 2316 82-83 -3.818328673543327 2316 84-85 -3.390523158508106 2316 86-87 -3.4248124850468784 2316 88-89 -3.196787452750115 2316 90-91 -2.3839576009352754 2316 92-93 -2.391893769682248 2316 94-95 -1.702768808116538 2316 96-97 -1.7778588714069983 2316 98-99 -2.006549370007143 2316 100-101 -1.6221083704355976 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13490.0 3 3976.0 4 4449.0 5 5221.0 6 6417.0 7 8133.0 8 10358.0 9 12610.0 10 15260.0 11 18084.0 12 21431.0 13 23829.0 14 25896.0 15 29291.0 16 32580.0 17 34133.0 18 30090.0 19 28756.0 20 30788.0 21 33088.0 22 36171.0 23 39933.0 24 44689.0 25 49581.0 26 54897.0 27 61219.0 28 68964.0 29 76872.0 30 87497.0 31 98502.0 32 112457.0 33 128325.0 34 147835.0 35 165444.0 36 161642.0 37 86239.0 38 9801.0 39 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.97284029198675 18.271497328679846 13.881839654289966 20.873822725043432 2 15.217014304660701 22.354810419064954 39.09753407975442 23.33064119651993 3 20.984834758511 26.821004770907763 30.398827622250725 21.795332848330514 4 12.035794947879408 16.346918012440163 39.05092794164169 32.56635909803874 5 12.440163455925276 39.39408959235149 33.5802244765335 14.585522475189725 6 28.90424746029936 37.031840389847694 18.07270862289033 15.991203526962611 7 26.58839992066815 35.00192820467617 21.248980806099738 17.160691068555938 8 25.8515723603061 31.651481020559157 20.553481405089574 21.943465214045165 9 27.042946339797457 17.392427585029235 21.474106328890798 34.09051974628251 10-11 24.951724905212277 25.931519313342545 27.267882989762267 21.848872791682915 12-13 28.228078442864575 24.30543945845857 27.002487867069185 20.46399423160767 14-15 24.074853466131945 25.075139923317614 28.35736084791327 22.49264576263717 16-17 20.969626827831377 28.48752195490888 28.382584582689407 22.160266634570334 18-19 20.656811844736836 28.07390262572142 30.79459700807628 20.47468852146546 20-21 22.9716929146875 25.46417117603624 29.852981694347395 21.71115421492886 22-23 21.792339715847103 25.90513557984811 30.06061748430138 22.241907220003412 24-25 22.653964501673588 25.784676417023604 30.49917126835772 21.062187812945083 26-27 22.40861469292558 24.92503707836027 31.8400375731059 20.826310655608253 28-29 21.00383248419669 27.172299173556 31.05624146441964 20.76762687782768 30-31 21.333974702666048 26.291171569512535 30.500336994559845 21.87451673326157 32-33 21.424320713480657 26.357670327768133 30.565764075192646 21.65224488355856 34-35 22.643310188515343 25.741210177987455 30.31276646994398 21.30271316355322 36-37 22.448319363160742 25.181472624652724 31.494587097766225 20.875620914420313 38-39 22.02774040981253 26.052596337247465 31.160125144871664 20.75953810806834 40-41 21.635938433864517 26.53504076660899 30.796073818300158 21.032946981226335 42-43 20.954896198848534 27.07765557773617 29.451835687096256 22.515612536319036 44-45 21.82899358614338 26.27341537176062 29.36402122409476 22.533569818001236 46-47 22.68550217926539 26.872966414063733 28.75974274414333 21.681788662527552 48-49 22.41481065343372 26.733785169885273 28.86890445853903 21.98249971814198 50-51 21.43447701674655 27.369528149609284 29.823574737788146 21.372420095856025 52-53 22.088225942509492 28.057363105068717 28.23073526950175 21.623675682920044 54-55 20.89174538576494 26.99058679504359 29.046106506526375 23.07156131266509 56-57 22.296904482635522 27.266905847149616 27.993737018164577 22.442452652050285 58-59 23.200661083425135 26.624148502394746 27.95083426237341 22.22435615180671 60-61 22.01926407624282 26.884224571741775 29.614418102598993 21.48209324941641 62-63 22.105874370776355 26.806153646038585 29.105586111526016 21.982385871659044 64-65 21.6229926763929 28.110025499533492 28.565760083944003 21.701221740129608 66-67 21.68611514986502 27.205508701111018 28.01948449210358 23.088891656920378 68-69 21.311438820949977 27.577243102327564 28.488583718707282 22.622734358015176 70-71 22.427830664967612 27.461440608837133 27.757057096118487 22.353671630076764 72-73 22.044106349849073 26.546474515789104 28.714828608073017 22.694590526288806 74-75 21.239171565851272 28.776048488003454 27.77919010564151 22.205589840503762 76-77 22.098296018528732 26.912595125179223 27.856071467960735 23.133037388331314 78-79 21.814575529012018 27.33252881102466 28.332722635282025 22.5201730246813 80-81 22.337395207640864 27.674447138880808 27.72300213823975 22.265155515238575 82-83 21.876358687785714 27.211747278351066 28.274628830965387 22.637265202897837 84-85 21.55305303084382 27.55374702990177 27.908463784301347 22.98473615495307 86-87 21.827614618028115 27.77300747264088 27.66839766721512 22.73098024211588 88-89 21.87736892913239 27.338276050846627 27.166458913017472 23.617896107003514 90-91 21.76085873821422 26.742465355477236 27.903176417442555 23.593499488865987 92-93 22.147602173685964 27.815948319698414 27.756664471887298 22.27978503472832 94-95 21.810949893681787 27.522245211268846 27.538733357449775 23.12807153759959 96-97 20.90779738111609 27.393171551320656 28.03527442513162 23.663756642431636 98-99 21.81409620005666 27.873653187345127 26.528185310718257 23.78406530187996 100-101 21.72131339726447 27.16123417525112 27.30177601299771 23.815676414486706 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4935.0 1 3569.5 2 2073.5 3 1731.5 4 1510.0 5 1593.0 6 2243.5 7 4855.5 8 6595.0 9 5639.5 10 4529.0 11 3969.5 12 3854.5 13 3862.0 14 4000.5 15 4208.0 16 4341.5 17 4516.5 18 4769.5 19 4989.0 20 5451.0 21 6087.0 22 6864.5 23 7769.0 24 8713.0 25 10131.5 26 11958.5 27 14008.5 28 15927.5 29 17870.5 30 20251.0 31 22914.0 32 25931.0 33 28802.5 34 31556.0 35 34097.5 36 37387.5 37 40503.5 38 41879.0 39 42767.5 40 43743.5 41 45306.5 42 47540.0 43 50594.0 44 53819.0 45 57421.0 46 67472.0 47 97737.0 48 118795.5 49 101511.0 50 76027.5 51 63183.0 52 58077.5 53 55100.5 54 52364.5 55 48806.0 56 44519.5 57 40400.5 58 37038.5 59 33327.5 60 28787.5 61 23902.5 62 18902.5 63 14036.0 64 9936.0 65 6977.0 66 4931.0 67 3535.5 68 2475.5 69 1677.0 70 1115.5 71 775.5 72 562.5 73 424.5 74 319.0 75 229.0 76 143.0 77 75.0 78 53.0 79 33.0 80 20.0 81 16.5 82 15.0 83 11.0 84 7.5 85 7.0 86 7.0 87 8.0 88 7.5 89 7.0 90 4.5 91 4.0 92 4.0 93 1.5 94 3.0 95 3.5 96 1.5 97 1.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 53.680151111443585 2 0.08773396641136724 3 0.12095736184238029 4 0.19526995658956348 5 0.12189245740914723 6 0.14978030754743135 7 0.15599594278535267 8 0.15374071230079714 9 0.14251956549959405 10-11 0.1298132669158788 12-13 0.14221703458093415 14-15 0.14939526819640966 16-17 0.14452727068706422 18-19 0.15085291716813456 20-21 0.17909830384665312 22-23 0.19722265615545906 24-25 0.22439543321327438 26-27 0.21630960684181924 28-29 0.21443941570828537 30-31 0.2292909335334071 32-33 0.22087507343250481 34-35 0.20720617647123538 36-37 0.1944448722659456 38-39 0.19026444502628168 40-41 0.19029194783706896 42-43 0.1841863238422967 44-45 0.20038547939599427 46-47 0.2245329472672107 48-49 0.22769577050774592 50-51 0.23864188920107635 52-53 0.26647473371778596 54-55 0.25225578054077125 56-57 0.2290159054255345 58-59 0.1869641077318102 60-61 0.1970576392907355 62-63 0.20115555809803762 64-65 0.18993441129683453 66-67 0.19967040631552546 68-69 0.18792670610936438 70-71 0.20088052999016498 72-73 0.22219520835029338 74-75 0.25297085362124005 76-77 0.2528058367565165 78-79 0.2473877830314258 80-81 0.2523932945947076 82-83 0.24397743449380527 84-85 0.21300926954734775 86-87 0.2262106187252337 88-89 0.22821832391270386 90-91 0.21410938197883822 92-93 0.21135910090011198 94-95 0.2514857018387279 96-97 0.2818763077586529 98-99 0.298653022338883 100-101 0.295297679422837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1817996.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.21478827207537 #Duplication Level Percentage of deduplicated Percentage of total 1 82.1836484142575 54.41772879176743 2 10.50964509907708 13.917878501000866 3 3.403048861729774 6.759964795768922 4 1.4552363047499872 3.85432655219431 5 0.7660255879845456 2.5361111059694363 6 0.4614159004618661 1.833153369267088 7 0.2874897447778778 1.3325250806602114 8 0.1940624712790577 1.027984435783881 9 0.14395994437762066 0.8579049512961506 >10 0.5574355192379109 6.064653220952862 >50 0.023057999864377853 1.0651084607721604 >100 0.01059411647937979 1.2696661115529317 >500 0.0019353646218046342 0.9539737823769281 >1k 0.0022409485094579975 3.1532934299834663 >5k 2.0372259176890884E-4 0.9557274106533562 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8193 0.4506610575600826 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5977 0.32876860015093545 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4556 0.25060561189353553 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4272 0.23498401536637042 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3379 0.1858639953003197 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3299 0.1814635455743577 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3196 0.17579796655218163 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3045 0.16749211769442837 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2555 0.14053936312291115 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2390 0.13146343556311454 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2145 0.11798705827735594 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2121 0.11666692335956735 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.7502810787262458E-5 0.0 12-13 0.0 0.0 0.0 5.5005621574524916E-5 0.0 14-15 0.0 0.0 0.0 1.6501686472357473E-4 0.0 16-17 0.0 0.0 0.0 4.4004497259619933E-4 0.0 18-19 0.0 0.0 0.0 6.325646481070365E-4 0.0 20-21 0.0 0.0 0.0 0.0011001124314904984 0.0 22-23 0.0 0.0 0.0 0.002310236106130047 0.0 24-25 0.0 0.0 0.0 0.0039053991317912692 0.0 26-27 0.0 0.0 0.0 0.005473059346665229 0.0 28-29 0.0 0.0 0.0 0.009791000640265436 0.0 30-31 0.0 0.0 0.0 0.02183723176508639 0.0 32-33 0.0 0.0 0.0 0.045984699636302834 0.0 34-35 0.0 0.0 0.0 0.08110578901163698 0.0 36-37 5.5005621574524916E-5 0.0 0.0 0.13275606767011588 0.0 38-39 5.5005621574524916E-5 0.0 0.0 0.20577603031029773 0.0 40-41 5.5005621574524916E-5 0.0 0.0 0.2888345188878303 0.0 42-43 5.5005621574524916E-5 0.0 0.0 0.3726630861674063 0.0 44-45 5.5005621574524916E-5 0.0 0.0 0.46600762597937506 0.0 46-47 5.5005621574524916E-5 0.0 0.0 0.5784941220992785 0.0 48-49 5.5005621574524916E-5 0.0 0.0 0.7013216750751927 0.0 50-51 5.5005621574524916E-5 0.0 0.0 0.8353428720415227 0.0 52-53 5.5005621574524916E-5 0.0 0.0 0.9617182876089936 0.0 54-55 5.5005621574524916E-5 0.0 0.0 1.0993973584100294 0.0 56-57 5.5005621574524916E-5 5.5005621574524916E-5 0.0 1.2693372262645242 0.0 58-59 5.5005621574524916E-5 8.250843236178737E-5 0.0 1.453111007945012 0.0 60-61 5.5005621574524916E-5 1.375140539363123E-4 0.0 1.6727484548920901 0.0 62-63 5.5005621574524916E-5 1.6501686472357476E-4 0.0 1.8866378143846303 0.0 64-65 5.5005621574524916E-5 1.6501686472357476E-4 0.0 2.0894160383191163 0.0 66-67 5.5005621574524916E-5 1.6501686472357476E-4 0.0 2.3215397613636113 0.0 68-69 5.5005621574524916E-5 1.6501686472357476E-4 0.0 2.59552276242632 0.0 70-71 5.5005621574524916E-5 1.6501686472357476E-4 0.0 2.8956059309261404 0.0 72-73 5.5005621574524916E-5 1.6501686472357476E-4 0.0 3.1976968046134315 0.0 74-75 5.5005621574524916E-5 1.6501686472357476E-4 0.0 3.5143916708287586 0.0 76-77 5.5005621574524916E-5 1.6501686472357476E-4 0.0 3.8290238262350416 0.0 78-79 5.5005621574524916E-5 1.6501686472357476E-4 0.0 4.217473525794336 0.0 80-81 5.5005621574524916E-5 1.6501686472357476E-4 0.0 4.64481219980682 0.0 82-83 5.5005621574524916E-5 1.6501686472357476E-4 0.0 5.060737207342591 0.0 84-85 5.5005621574524916E-5 1.6501686472357476E-4 0.0 5.50540265215105 0.0 86-87 5.5005621574524916E-5 1.6501686472357476E-4 0.0 5.97025516007736 0.0 88-89 1.1001124314904983E-4 1.6501686472357476E-4 0.0 6.448309017181556 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9320 0.0 37.08991 1 TACATGG 11375 0.0 29.738745 2 ACATGGG 11270 0.0 28.507423 3 ATGGGAG 2835 0.0 27.637398 5 CATGGGA 7930 0.0 26.151972 4 CATGGGG 3370 0.0 24.25098 4 GAGTACT 9460 0.0 23.774961 12-13 GTATAGT 390 2.13171E-7 23.531513 1 ATGGGAT 3010 0.0 22.423927 5 AGTACTT 10055 0.0 22.368092 12-13 GTACTTT 10500 0.0 21.580715 14-15 CCTTTAG 655 2.0918378E-10 20.238298 1 GTATCGC 140 5.064095E-4 20.229134 6 GTATAGG 355 6.429204E-5 20.106739 1 TACTTTT 11655 0.0 19.178808 14-15 ACTTTTT 12520 0.0 19.176367 16-17 TTTAGGC 450 0.0 18.898745 3 ATGGGGA 1890 0.0 18.730164 5 TACCTGG 1060 0.0 18.719936 2 TTTGCGT 490 0.0 18.320211 3 >>END_MODULE