##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939580_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1817996 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.47078431415691 31.0 31.0 34.0 30.0 34.0 2 31.64275553961615 31.0 31.0 34.0 30.0 34.0 3 31.684104915522365 31.0 31.0 34.0 30.0 34.0 4 35.47089707568114 37.0 35.0 37.0 33.0 37.0 5 35.24853520029747 37.0 35.0 37.0 33.0 37.0 6 35.2812002886695 37.0 35.0 37.0 32.0 37.0 7 35.191035623840754 37.0 35.0 37.0 32.0 37.0 8 35.16398660943148 37.0 35.0 37.0 32.0 37.0 9 36.72426947033987 39.0 35.0 39.0 32.0 39.0 10-11 36.60937895352905 39.0 35.0 39.0 32.0 39.0 12-13 36.55221821170123 39.0 35.0 39.0 32.0 39.0 14-15 37.49553959414652 39.0 36.0 41.0 32.0 41.0 16-17 37.488215595633875 39.0 36.0 41.0 32.0 41.0 18-19 37.54400147195044 39.0 36.0 41.0 32.0 41.0 20-21 37.49825824699285 39.0 36.0 41.0 32.0 41.0 22-23 37.360951564249866 39.0 36.0 41.0 31.5 41.0 24-25 37.18926609299471 39.0 36.0 40.5 31.0 41.0 26-27 36.84492787662899 39.0 35.5 40.0 30.5 41.0 28-29 36.65723576949564 38.5 35.0 40.0 30.0 41.0 30-31 36.39480174873872 38.0 35.0 40.0 30.0 41.0 32-33 36.15742416374954 38.0 35.0 40.0 30.0 41.0 34-35 36.065380781915906 38.0 35.0 40.0 30.0 41.0 36-37 35.909708547213526 38.0 35.0 40.0 30.0 41.0 38-39 35.888610591002404 38.0 35.0 40.0 29.5 41.0 40-41 35.77808367015109 38.0 35.0 40.0 29.0 41.0 42-43 35.556582357716955 38.0 34.5 40.0 27.5 41.0 44-45 35.26518100149835 38.0 34.0 40.0 27.0 41.0 46-47 34.83933297983054 38.0 34.0 40.0 25.5 41.0 48-49 34.41512935121969 38.0 33.0 40.0 24.0 41.0 50-51 34.13181024600714 38.0 33.0 40.0 23.0 41.0 52-53 33.889718679249015 38.0 33.0 40.0 23.0 41.0 54-55 33.620795095258735 37.0 33.0 40.0 22.0 41.0 56-57 32.88224974092353 36.5 31.5 40.0 17.0 41.0 58-59 29.9389795137063 35.0 25.5 39.0 7.5 40.0 60-61 29.61267819071109 34.0 25.5 38.0 7.5 40.0 62-63 31.26762847663031 35.0 29.5 38.5 8.0 40.0 64-65 31.261556681092806 35.0 30.5 38.5 4.0 40.0 66-67 26.581282357056892 31.5 18.5 36.5 2.0 39.5 68-69 19.315599154233563 19.0 7.5 32.0 2.0 36.5 70-71 17.37055554577678 15.0 7.0 29.0 2.0 35.0 72-73 19.07034586434734 19.5 7.5 30.5 2.0 35.0 74-75 20.483076145382057 22.5 7.5 32.0 2.0 35.5 76-77 22.227991975779922 27.0 11.0 33.0 2.0 35.5 78-79 24.36024639218128 30.5 12.5 34.5 2.0 36.0 80-81 25.884697216055482 31.5 21.0 35.0 2.0 36.0 82-83 26.89792551798794 33.0 26.0 35.0 2.0 36.0 84-85 26.914118072867048 33.0 26.0 35.0 2.0 36.0 86-87 26.68093934200075 33.0 25.0 35.0 2.0 35.0 88-89 26.23410255028064 33.0 24.5 35.0 2.0 35.0 90-91 24.515556964921814 31.5 11.0 34.5 2.0 35.0 92-93 23.076095326942415 31.0 2.0 34.0 2.0 35.0 94-95 19.478991702952044 26.5 2.0 34.0 2.0 35.0 96-97 16.855309637644968 16.5 2.0 33.0 2.0 35.0 98-99 17.139599042022095 19.5 2.0 33.0 2.0 35.0 100-101 16.38476624811056 9.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.19406767944801473 1101 2 0.22024351620580163 1101 3 0.3056414545696313 1101 4 0.22077581653051936 1101 5 0.28103599491936393 1101 6 0.28284478171040206 1101 7 0.34208331708669704 1101 8 0.4892357798758695 1101 9 0.3403869394585257 1101 10-11 0.30767366755360115 1101 12-13 0.3921018612891132 1101 14-15 0.4697461523437241 1101 16-17 0.40351913742745893 1101 18-19 0.41948251995841446 1101 20-21 0.3457721879656077 1101 22-23 0.4270618532915478 1101 24-25 0.5333614632761012 1101 26-27 0.4445494754824608 1101 28-29 0.36312910965982326 1101 30-31 0.23424925464880886 1101 32-33 0.38027663695584835 1101 34-35 0.23123148354660117 1101 36-37 0.1196952694053266 1101 38-39 0.11087901227274699 1101 40-41 0.17864804046309501 1101 42-43 0.39328810332946773 1101 44-45 0.23663538254061933 1101 46-47 0.27350656038630206 1101 48-49 0.25091430866434905 1101 50-51 0.2158720560435583 1101 52-53 0.16169914179262435 1101 54-55 0.17507029784884054 1101 56-57 -3.3230898465303405 1101 58-59 -10.33023409818027 1101 60-61 -11.343535364493604 1101 62-63 -3.8374683565505663 1101 64-65 -1.1039945042345742 1101 66-67 -2.141782381515778 1101 68-69 -4.566042364254308 1101 70-71 -4.678553514081347 1101 72-73 -3.4502466182270677 1101 74-75 -6.137138211081261 1101 76-77 -5.027178924165003 1101 78-79 1.029123163067048 1101 80-81 1.7539686240235604 1101 82-83 0.5406924477556032 1101 84-85 0.10644957048108594 1101 86-87 0.1918428716836722 1101 88-89 0.13024036311152898 1101 90-91 1.5291367374521734 1101 92-93 1.3638008789734073 1101 94-95 -8.388591902513832 1101 96-97 -9.46646193884295 1101 98-99 -8.608219198041734 1101 100-101 -9.650372174296475 1102 1 0.2796063297157616 1102 2 0.2642584183932719 1102 3 0.19040085393777773 1102 4 0.21399695766982063 1102 5 0.2597122573170054 1102 6 0.18616245507136142 1102 7 0.1860032705129413 1102 8 0.4041686358557257 1102 9 0.2785319126191652 1102 10-11 0.19581469677933683 1102 12-13 0.3395103723006372 1102 14-15 0.5499921302659345 1102 16-17 0.4192869303131843 1102 18-19 0.4618734922325629 1102 20-21 0.3845674100356362 1102 22-23 0.3967060632713526 1102 24-25 0.3932998260486542 1102 26-27 0.08428294200699327 1102 28-29 0.3011353092900393 1102 30-31 0.20371834214588347 1102 32-33 0.257281053574296 1102 34-35 0.22833254422963734 1102 36-37 0.25356993233133807 1102 38-39 0.31791587511387576 1102 40-41 0.10790576836808441 1102 42-43 0.2750373090974989 1102 44-45 0.06456871355860727 1102 46-47 -0.034959772831463454 1102 48-49 0.3153941124800781 1102 50-51 0.26292982173968227 1102 52-53 0.048843444484326426 1102 54-55 -0.0487752329371034 1102 56-57 -3.3543696651799735 1102 58-59 -9.911527426278571 1102 60-61 -7.915458020986883 1102 62-63 -2.5047368166676733 1102 64-65 -0.7762541022710785 1102 66-67 -1.8222815659453602 1102 68-69 -4.955232215273638 1102 70-71 -4.860901638740298 1102 72-73 -3.46916543850784 1102 74-75 -4.178016949210459 1102 76-77 -4.369292908592936 1102 78-79 1.3859331613540853 1102 80-81 1.865268821632359 1102 82-83 0.6400157391635375 1102 84-85 0.11111489228556692 1102 86-87 0.08576073541307139 1102 88-89 -0.05825382488231057 1102 90-91 1.1665778653940144 1102 92-93 0.7591674029716629 1102 94-95 -8.568675649322628 1102 96-97 -9.271684679924803 1102 98-99 -8.21980503660765 1102 100-101 -9.37220776989834 1103 1 0.22938128770810806 1103 2 0.221613280754692 1103 3 0.24819597560418316 1103 4 0.26168441735342185 1103 5 0.25073191066753964 1103 6 0.22614649974179457 1103 7 0.24338491222765413 1103 8 0.2678086741889345 1103 9 0.2979410489260701 1103 10-11 0.3729792067817357 1103 12-13 0.37907953348933177 1103 14-15 0.45105898152487356 1103 16-17 0.5089426050939423 1103 18-19 0.37510951040658824 1103 20-21 0.4373562803792126 1103 22-23 0.37337293766046287 1103 24-25 0.41092549672548984 1103 26-27 0.5771746237335407 1103 28-29 0.5531203218347969 1103 30-31 0.4451135801368622 1103 32-33 0.3630545867029369 1103 34-35 0.44089646800560445 1103 36-37 0.5146297490976792 1103 38-39 0.47822777878904077 1103 40-41 0.44784706151329345 1103 42-43 0.42852693392269003 1103 44-45 0.4410792627711402 1103 46-47 0.3899348080267444 1103 48-49 0.30152400750164077 1103 50-51 0.03771658341971573 1103 52-53 0.1421004370113792 1103 54-55 0.09792032399204231 1103 56-57 0.6199837706335103 1103 58-59 -8.484416952182961 1103 60-61 -7.557432243192636 1103 62-63 -2.3245190674021394 1103 64-65 -0.26854044148857525 1103 66-67 -1.7437811479741114 1103 68-69 -3.408494805078508 1103 70-71 -3.9060602179861323 1103 72-73 -3.0979348289771984 1103 74-75 -3.688687847800921 1103 76-77 -0.6043242606471146 1103 78-79 3.3076154829948905 1103 80-81 2.737451164887524 1103 82-83 1.3141143781323805 1103 84-85 0.9059822390467538 1103 86-87 0.7659808706796838 1103 88-89 0.7511535317484999 1103 90-91 2.0487823956521574 1103 92-93 1.6709565220207665 1103 94-95 -6.761456536387303 1103 96-97 -8.496203451577763 1103 98-99 -7.759104298968396 1103 100-101 -9.120990347936015 1104 1 0.2113048443070369 1104 2 0.18004055844688338 1104 3 0.20138600812808605 1104 4 0.08200900428354174 1104 5 0.1769002035293994 1104 6 0.09426329234688069 1104 7 0.15791225594580993 1104 8 0.177239791169022 1104 9 0.06751530368850212 1104 10-11 0.11315315668350934 1104 12-13 0.24691754977573055 1104 14-15 0.27365616604708265 1104 16-17 0.2780779094247592 1104 18-19 0.27155796531737764 1104 20-21 0.31018577850032614 1104 22-23 0.2700993537545031 1104 24-25 0.2258934211461252 1104 26-27 0.2349424568590166 1104 28-29 0.29830327898312703 1104 30-31 0.39114224029875544 1104 32-33 0.4014446127057667 1104 34-35 0.40517275000986075 1104 36-37 0.19578178000761426 1104 38-39 0.13563165394290166 1104 40-41 0.21425410754399365 1104 42-43 0.21268518431599404 1104 44-45 0.09906269921911104 1104 46-47 0.07711843358613635 1104 48-49 -0.13233142007764798 1104 50-51 0.05780559620919945 1104 52-53 0.07849215849589797 1104 54-55 0.16553336289845078 1104 56-57 0.6824325189930533 1104 58-59 -7.889588986516468 1104 60-61 -7.454817696285666 1104 62-63 -2.089411184227348 1104 64-65 -0.2837186708513819 1104 66-67 -1.6460794223323951 1104 68-69 -3.0517846101796273 1104 70-71 -3.6217638021366376 1104 72-73 -2.7615471831698457 1104 74-75 -3.286202907428077 1104 76-77 -0.2502754829427012 1104 78-79 3.304162976861001 1104 80-81 2.7175940581292366 1104 82-83 1.1290990128294354 1104 84-85 0.7795001190425168 1104 86-87 0.7744407537892606 1104 88-89 0.5894616366401273 1104 90-91 2.0650557594715515 1104 92-93 1.7601710575158371 1104 94-95 -5.917085046564271 1104 96-97 -7.180722399400942 1104 98-99 -5.3705459474983 1104 100-101 -4.949485444896375 1105 1 0.21876322726800979 1105 2 0.2538642997762466 1105 3 0.2530260262547088 1105 4 0.1431243262249069 1105 5 0.35531428454576997 1105 6 0.29952883532342156 1105 7 0.300572244102014 1105 8 0.16831229631893763 1105 9 0.17700972333583564 1105 10-11 0.3423443698170203 1105 12-13 0.26484766603475407 1105 14-15 0.29648675298334837 1105 16-17 0.36737558304130147 1105 18-19 0.3591029978076037 1105 20-21 0.29891826512258035 1105 22-23 0.3846152219007095 1105 24-25 0.4268271560456256 1105 26-27 0.407610960249734 1105 28-29 0.44367825007543615 1105 30-31 0.49780337273136155 1105 32-33 0.37259895500749707 1105 34-35 0.37235193487332907 1105 36-37 0.302522717699361 1105 38-39 0.43498946890966295 1105 40-41 0.3666798799639963 1105 42-43 0.4196889312865366 1105 44-45 0.3123679870861622 1105 46-47 0.19012308154067625 1105 48-49 0.2860069462004162 1105 50-51 0.3570512560462973 1105 52-53 0.3989779145270802 1105 54-55 0.25146197127920544 1105 56-57 0.8740712805544533 1105 58-59 -7.94497824769622 1105 60-61 -7.08688350287354 1105 62-63 -1.642552797790767 1105 64-65 0.03735138800666604 1105 66-67 -1.1882756844519022 1105 68-69 -3.0673739058480862 1105 70-71 -3.5731498336764247 1105 72-73 -2.7647157326791856 1105 74-75 -3.296597375332862 1105 76-77 0.21651657289157455 1105 78-79 3.923919256679355 1105 80-81 3.405111285772069 1105 82-83 1.916825712567828 1105 84-85 1.5240742303233965 1105 86-87 1.3832654509102547 1105 88-89 1.3368095488035472 1105 90-91 2.619878630562667 1105 92-93 2.1621389188552023 1105 94-95 -6.1250674439570325 1105 96-97 -7.58093387665377 1105 98-99 -5.558055956574979 1105 100-101 -4.746729556752069 1106 1 0.15416803178258576 1106 2 0.15998158730176115 1106 3 0.19553093982960235 1106 4 0.13094015227822808 1106 5 0.2031306540359452 1106 6 0.14435390459829733 1106 7 0.15087510359215628 1106 8 0.07497355348018431 1106 9 0.2390989823064018 1106 10-11 0.19253361370988387 1106 12-13 0.15355699284968694 1106 14-15 0.10748194569831071 1106 16-17 0.2617836710865973 1106 18-19 0.21850136525936392 1106 20-21 0.16676680927123755 1106 22-23 0.19452926830489048 1106 24-25 0.1055704636473962 1106 26-27 0.11344978966543806 1106 28-29 0.30652451125651226 1106 30-31 0.2923441499419681 1106 32-33 0.369341264177244 1106 34-35 0.40069243322623294 1106 36-37 0.4003874370861453 1106 38-39 0.43838381812538074 1106 40-41 0.3512332201559971 1106 42-43 0.2581363428488501 1106 44-45 0.15706119300664767 1106 46-47 0.3169315408279303 1106 48-49 0.2084161509551734 1106 50-51 0.1641153224564107 1106 52-53 0.3413673477703938 1106 54-55 0.3261409839077274 1106 56-57 0.8634149899798302 1106 58-59 -8.342222330228296 1106 60-61 -7.5112073901732614 1106 62-63 -1.8933351870268815 1106 64-65 -0.33685949639419377 1106 66-67 0.8376892755921688 1106 68-69 -1.852650017056579 1106 70-71 -2.166639087212628 1106 72-73 -0.8379185417149415 1106 74-75 -1.0226479266815183 1106 76-77 2.2720820588972757 1106 78-79 4.438194330562794 1106 80-81 3.5257053847937243 1106 82-83 1.8623044777727387 1106 84-85 1.6128337184911246 1106 86-87 1.3809013673978647 1106 88-89 1.3173543762170574 1106 90-91 2.6714388004772545 1106 92-93 2.3941754005171383 1106 94-95 -5.50843164308295 1106 96-97 -7.098265782843782 1106 98-99 -5.0478894251459465 1106 100-101 -4.296161532043804 1107 1 0.047132551233765696 1107 2 0.06578569264710055 1107 3 0.0680221984256626 1107 4 0.07403018666246908 1107 5 0.1409005786231674 1107 6 0.06701116681549735 1107 7 0.10566273523230052 1107 8 0.16705469281109941 1107 9 0.15748935970250244 1107 10-11 0.09354525991764717 1107 12-13 0.04170413704755305 1107 14-15 0.0259382469839764 1107 16-17 0.09309611710167331 1107 18-19 0.03856833770213797 1107 20-21 0.1258927000223764 1107 22-23 0.04036158076958429 1107 24-25 0.03175517304243414 1107 26-27 -0.038026957369545755 1107 28-29 -0.0022212010952316064 1107 30-31 -0.026227336470441287 1107 32-33 0.10234535872560002 1107 34-35 0.013083321402710624 1107 36-37 0.14281267835720968 1107 38-39 -0.035178366270486094 1107 40-41 0.07846482168086055 1107 42-43 -0.0766983594866204 1107 44-45 -0.05671778235961966 1107 46-47 0.30570683068541626 1107 48-49 0.2901093463639626 1107 50-51 0.10451649268330954 1107 52-53 0.1531016962970213 1107 54-55 0.24506965322103014 1107 56-57 0.8738517279619558 1107 58-59 -8.425676524025846 1107 60-61 -7.755251919424783 1107 62-63 -2.074563852602779 1107 64-65 -0.2504003152208085 1107 66-67 0.5161442064914752 1107 68-69 -2.372583169194378 1107 70-71 -2.0726381092550845 1107 72-73 -1.091785211426913 1107 74-75 -0.8241230645506938 1107 76-77 2.341422311297528 1107 78-79 4.33718829048107 1107 80-81 3.252858863914021 1107 82-83 1.7196711350266511 1107 84-85 1.4210510015548117 1107 86-87 1.3002600992506252 1107 88-89 1.1343073532776238 1107 90-91 2.308589559588249 1107 92-93 1.6624669439874573 1107 94-95 -6.752148369686719 1107 96-97 -7.99780140873661 1107 98-99 -6.007797578114287 1107 100-101 -5.381549320240211 1108 1 -0.010708800962422771 1108 2 -0.04872731450703682 1108 3 -0.018881321441568844 1108 4 0.0350293689318093 1108 5 -0.02404536253244771 1108 6 0.048272848682017866 1108 7 0.043766969707505154 1108 8 0.049919308034588994 1108 9 0.08431555769805499 1108 10-11 0.0558896191874112 1108 12-13 0.09253511050957286 1108 14-15 -0.02687134261996249 1108 16-17 -0.06658517942437925 1108 18-19 -0.045511141286617374 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0.020404002271540378 2313 58-59 2.972309401749662 2313 60-61 2.9117244188594285 2313 62-63 0.262485902754225 2313 64-65 -0.43414158833135374 2313 66-67 -2.202720621001369 2313 68-69 -4.633051689539393 2313 70-71 -3.3473321846399386 2313 72-73 -2.825403075200409 2313 74-75 -3.672323778173343 2313 76-77 0.30791238536730603 2313 78-79 2.8350182926083995 2313 80-81 2.1296755557218603 2313 82-83 0.7316513895327219 2313 84-85 0.4137294797400024 2313 86-87 0.3649153508557923 2313 88-89 0.4200306697857066 2313 90-91 1.767719674850877 2313 92-93 3.151398540645612 2313 94-95 5.167686206200958 2313 96-97 1.1529269601811585 2313 98-99 2.639892213600728 2313 100-101 3.7459414693861532 2314 1 -0.33624667771981365 2314 2 -0.3164590949217434 2314 3 -0.44820370637370743 2314 4 -0.11326672521794023 2314 5 -0.2510408061132594 2314 6 -0.30024984813336175 2314 7 -0.30686873605015563 2314 8 -0.4640925337821358 2314 9 -0.5427437244831737 2314 10-11 -0.47576409295410826 2314 12-13 -0.5166861560287686 2314 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3.326059995831187 2314 80-81 2.70633988279679 2314 82-83 1.0812437156718104 2314 84-85 0.829353273388012 2314 86-87 0.8704929375669117 2314 88-89 1.0640459941116092 2314 90-91 2.35094597186205 2314 92-93 3.8118916096050803 2314 94-95 6.4747313018317705 2314 96-97 3.974402072774012 2314 98-99 5.908609532428592 2314 100-101 6.393777931923438 2315 1 -0.45359812640208474 2315 2 -0.3729064910352058 2315 3 -0.5054316838308566 2315 4 -0.24338014102132632 2315 5 -0.45014610742514094 2315 6 -0.45883413829074016 2315 7 -0.4449221934783054 2315 8 -0.5778455445624786 2315 9 -0.6140665970599386 2315 10-11 -0.44210064558137674 2315 12-13 -0.6273624416027417 2315 14-15 -0.7856343916514703 2315 16-17 -0.7488810714518124 2315 18-19 -0.7628156488595792 2315 20-21 -0.8977970577328591 2315 22-23 -0.6863328471368817 2315 24-25 -0.7454971214399819 2315 26-27 -0.6458103655747891 2315 28-29 -0.7118795319800881 2315 30-31 -0.662566129267276 2315 32-33 -0.6801572315072875 2315 34-35 -0.7390415394206045 2315 36-37 -0.661808132810954 2315 38-39 -0.9851634337711843 2315 40-41 -0.8933661540250668 2315 42-43 -0.6270498772857778 2315 44-45 -0.7266054068454437 2315 46-47 -0.6898124530185399 2315 48-49 -0.6816740289801118 2315 50-51 -0.6250134187075318 2315 52-53 -0.5446727330785421 2315 54-55 -0.46140815196252305 2315 56-57 0.2660622791016607 2315 58-59 3.0669507356624024 2315 60-61 2.972498656612082 2315 62-63 0.5212392452384371 2315 64-65 -0.11399000106808543 2315 66-67 -2.087744912210777 2315 68-69 -4.929121525904243 2315 70-71 -3.5237510878682663 2315 72-73 -2.805567859959371 2315 74-75 -0.7538518826312917 2315 76-77 1.3523933490144096 2315 78-79 3.038385838265068 2315 80-81 2.0099979516881206 2315 82-83 0.41780172142444627 2315 84-85 0.21094179617640307 2315 86-87 0.08626264502429493 2315 88-89 0.18278602638249808 2315 90-91 1.9723270723461113 2315 92-93 3.318635757583472 2315 94-95 5.7709605186781125 2315 96-97 3.623670365456938 2315 98-99 5.673074373529545 2315 100-101 5.799077139182053 2316 1 -0.4666249101082549 2316 2 -0.394257694763013 2316 3 -0.49742390561795347 2316 4 -0.3009083698713937 2316 5 -0.397268787965686 2316 6 -0.5377203227895393 2316 7 -0.42403477687035007 2316 8 -0.25941657220950276 2316 9 -0.6155678517531058 2316 10-11 -0.4614705097560119 2316 12-13 -0.636692315779527 2316 14-15 -0.5515767246590002 2316 16-17 -0.8353115962809525 2316 18-19 -0.7150481365885497 2316 20-21 -0.5604473403589623 2316 22-23 -0.8825323771100528 2316 24-25 -0.6622097322122258 2316 26-27 -0.5205842344301317 2316 28-29 -0.5383207062182933 2316 30-31 -0.5631608570880289 2316 32-33 -0.6366628702925539 2316 34-35 -0.5858697896440006 2316 36-37 -0.44747922951382435 2316 38-39 -0.58405590919768 2316 40-41 -0.6187323526051145 2316 42-43 -0.4782186813622573 2316 44-45 -0.5965592190498441 2316 46-47 -0.652680031085886 2316 48-49 -0.7866198343375999 2316 50-51 -0.639569512502419 2316 52-53 -0.6444004676880084 2316 54-55 -0.5237332713900358 2316 56-57 0.1682804946667531 2316 58-59 3.260814799471312 2316 60-61 3.1141763087317003 2316 62-63 0.5761108260307601 2316 64-65 -0.13362749741855495 2316 66-67 -1.8505217584304248 2316 68-69 -3.037547248022186 2316 70-71 -3.232589282507055 2316 72-73 -0.8592946409182076 2316 74-75 5.219982328374154 2316 76-77 5.404576035505958 2316 78-79 4.261758357574397 2316 80-81 2.7726257899977753 2316 82-83 1.1034098157203758 2316 84-85 0.7062951940456834 2316 86-87 0.5817721519617756 2316 88-89 0.6447516409446692 2316 90-91 2.189727051950655 2316 92-93 4.001190819498586 2316 94-95 6.334667453645938 2316 96-97 3.8004552124700517 2316 98-99 6.145638874164458 2316 100-101 6.12774516678763 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 30.0 5 128.0 6 272.0 7 646.0 8 1317.0 9 2266.0 10 3718.0 11 5789.0 12 8425.0 13 11251.0 14 13758.0 15 18089.0 16 23866.0 17 31538.0 18 31450.0 19 28065.0 20 28183.0 21 28862.0 22 28683.0 23 29193.0 24 30475.0 25 32649.0 26 40304.0 27 56025.0 28 78408.0 29 102705.0 30 124092.0 31 138642.0 32 145980.0 33 146904.0 34 151005.0 35 159904.0 36 156698.0 37 114282.0 38 41940.0 39 2444.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.01748237320121 19.154793966586798 14.562885650699156 21.264838009512832 2 15.423314726193377 22.934808261372094 37.94442723768417 23.697449774750364 3 20.884458851051683 26.63310584804232 30.861064921592106 21.62137037931389 4 12.31795889539911 16.96851918266047 38.46972160554809 32.24380031639234 5 12.27207304890567 39.763383630594454 33.759305176774276 14.205238143725593 6 29.259580329109635 37.305362608058545 17.477706221575843 15.957350841255977 7 26.349177885979948 34.9594828591482 21.13882538795465 17.5525138669172 8 25.808637642767092 32.272128211503215 20.31247593504056 21.606758210689133 9 26.262213998270624 17.326715790353774 21.53574595323642 34.87532425813919 10-11 24.824752089663562 26.11323127223602 27.197392073469906 21.864624564630507 12-13 27.795523202471294 24.33448698456982 27.174179701165457 20.695810111793424 14-15 23.673396084022556 25.181189314780955 28.373454610261454 22.771959990935034 16-17 20.769737667189585 28.643187333745505 28.480673224803578 22.10640177426133 18-19 20.45789643768178 28.127723650465143 30.77092114219707 20.643458769656007 20-21 22.577029872452965 25.656822127221403 29.86224942189092 21.903898578434717 22-23 21.495234312946785 26.0249747524197 30.097645979419095 22.38214495521442 24-25 22.51484054935335 25.989910846692393 30.29336762758032 21.201880976373932 26-27 22.20149423058676 25.097518279090806 31.747160673105085 20.95382681721734 28-29 20.866332521807244 27.231110015874815 31.004513815929162 20.898043646388782 30-31 21.179978827282277 26.472058091028384 30.404825773396908 21.943137308292428 32-33 21.321931125228183 26.467337057973968 30.4990088803332 21.71172293646465 34-35 22.500990120794736 25.794324407577722 30.225610024422977 21.47907544720456 36-37 22.326270956667557 25.348914218424717 31.302180922031436 21.022633902876287 38-39 21.979566373778635 26.141130689276377 31.024662212654714 20.854640724290274 40-41 21.473304530500613 26.620186866084445 30.76455776201109 21.141950841403855 42-43 20.86528374609087 27.08908697678375 29.44924952716096 22.59637974996442 44-45 21.601534047834942 26.446662053071755 29.30486117648872 22.646942722604578 46-47 22.435709163878496 26.91977907487259 28.853300595528925 21.791211165719997 48-49 22.277141064174124 26.700630841893187 28.90945822461494 22.112769869317745 50-51 21.258718663657948 27.363592803626098 29.911095295066026 21.46659323764993 52-53 21.99225779181127 27.984227184028388 28.255069627312324 21.768445396848023 54-55 20.821069100792936 26.923050465121335 29.082472601358255 23.17340783272747 56-57 22.091877595616385 27.174730909060884 28.186281659983543 22.54710983533919 58-59 21.86500208700267 26.36497868265954 29.64582442975803 22.12419480057976 60-61 21.270395385899672 26.823432013080645 30.492524853912116 21.413647747107564 62-63 21.95726158630936 26.815499715887682 29.18840139652343 22.038837301279525 64-65 21.41104586385868 28.079604765020505 28.708745231252784 21.80060413986803 66-67 21.366634893340613 26.875089675300334 28.74066959782088 23.017605833538173 68-69 20.705682606190102 26.8092580564661 30.100654071200307 22.384405266143492 70-71 21.79233553877499 26.905083404556095 28.85692038867213 22.445660667996783 72-73 21.38422904076535 26.23200204550693 29.678249937758228 22.705518975969493 74-75 20.77502505144705 28.05455629896593 28.979614236966494 22.190804412620523 76-77 20.18165626894504 26.96416083237927 29.399599459591858 23.454583439083834 78-79 21.28685253344183 27.02759291563971 28.942703508851025 22.742851042067436 80-81 21.762260757980787 27.479802476418342 28.126172754996144 22.631764010604734 82-83 21.437784895524786 27.119441185352404 28.58394238475328 22.858831534369532 84-85 21.15899666055912 27.374256656741668 28.109665236315912 23.357081446383297 86-87 21.371708444572548 27.549022492988733 27.94339169616162 23.135877366277104 88-89 21.37719224904785 27.110895733543966 27.43317367034911 24.07873834705907 90-91 21.128746968070576 26.527719906289583 28.263735778560843 24.079797347078998 92-93 21.295379560657885 27.554257347822137 28.22694664659734 22.923416444922633 94-95 20.085875875625746 27.183893206282573 28.823281163669478 23.906949754422204 96-97 19.15978248772257 27.27412799623274 28.945961409075988 24.620128106968703 98-99 20.30244115232443 27.373988389067545 27.857103710637894 24.466466747970138 100-101 19.416527292183588 26.791541778186996 28.918764705540696 24.87316622408872 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2505.0 1 1843.0 2 1186.0 3 1244.0 4 1303.5 5 1442.0 6 1875.0 7 2743.5 8 3764.5 9 4456.0 10 4594.0 11 4462.0 12 4390.5 13 4414.5 14 4565.5 15 4669.0 16 4814.0 17 4989.5 18 5122.0 19 5389.0 20 5727.0 21 6178.5 22 6827.5 23 7537.0 24 8291.5 25 9398.5 26 11215.0 27 13255.5 28 15192.5 29 17346.5 30 19693.0 31 22400.0 32 25730.0 33 29038.5 34 32041.0 35 35228.5 36 38270.5 37 40861.5 38 42816.5 39 44212.5 40 45800.0 41 48246.0 42 50669.5 43 53735.0 44 58306.0 45 64286.0 46 73573.5 47 88711.5 48 104614.5 49 100126.0 50 81685.5 51 69873.5 52 62624.0 53 57598.5 54 53587.5 55 49221.5 56 44531.5 57 39897.0 58 35651.0 59 31485.0 60 26620.5 61 21550.0 62 16799.5 63 12442.5 64 8699.0 65 5979.0 66 4033.0 67 2706.0 68 1770.0 69 1085.5 70 709.0 71 474.5 72 342.0 73 293.5 74 212.5 75 137.5 76 76.0 77 27.5 78 15.0 79 8.5 80 2.5 81 1.0 82 1.0 83 0.5 84 1.0 85 1.5 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02480753533011074 2 0.012926321070013354 3 1.6501686472357473E-4 4 0.0 5 0.06221135800078768 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 4.4004497259619933E-4 16-17 0.0 18-19 4.675477833834618E-4 20-21 0.0 22-23 0.0 24-25 2.2002248629809966E-4 26-27 1.925196755108372E-4 28-29 0.001210123674639548 30-31 0.004978008752494505 32-33 0.005638076211388805 34-35 0.001980202376682897 36-37 0.08468115441398111 38-39 0.07874054728393241 40-41 0.08294847733438357 42-43 0.08660635116908948 44-45 0.08938413505860299 46-47 0.08490117690027921 48-49 0.07568773528654628 50-51 0.07015967031830654 52-53 0.05899352913867797 54-55 0.04892750039053992 56-57 0.06771192015824017 58-59 0.7027243184253431 60-61 0.3387521204667117 62-63 0.0029152979434498203 64-65 0.002172722052193734 66-67 1.0914215432817234 68-69 2.354625642740688 70-71 2.335731211729839 72-73 1.7942558729502154 74-75 1.2755528615024456 76-77 0.7389455202321678 78-79 0.5647702195164346 80-81 0.038173901372720295 82-83 2.750281078726246E-4 84-85 0.0014851517825121728 86-87 0.001622665836448485 88-89 0.0 90-91 0.33173890371595977 92-93 0.05286040233311844 94-95 0.9428513594089315 96-97 1.647913416751192 98-99 0.6377901821566164 100-101 0.5619649328161338 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1817996.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.206053462390386 #Duplication Level Percentage of deduplicated Percentage of total 1 69.81410062897599 28.069494623173107 2 14.242415371930214 11.452626277547937 3 5.79570236399749 6.990669572969563 4 3.000736591170894 4.825911032446721 5 1.7860298182615924 3.5904605179224482 6 1.158358699003592 2.7943819068458033 7 0.7883517023328176 2.2187557483811813 8 0.5485886819753754 1.7645268701091374 9 0.43284136974930815 1.5662558927587473 >10 2.226931843863008 15.81168656190775 >50 0.1270052919926165 3.5080464009226437 >100 0.06865963234059186 5.002881963477904 >500 0.003837121645092438 1.093207340258199 >1k 0.004659361997612245 4.565398266541712 >5k 0.0012333605287797123 3.442933676261987 >10k+ 5.481602350132054E-4 3.302763348475239 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21383 1.1761852061280662 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14952 0.8224440537822966 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11873 0.6530817449543344 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11735 0.6454909691770498 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8946 0.49208029060569985 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8755 0.4815742168849656 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7947 0.43712967465274954 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7770 0.4273936796340586 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6785 0.37321314238315156 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5981 0.3289886226372335 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5808 0.3194726501048407 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5434 0.2989005476359684 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5061 0.2783834507886706 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4436 0.24400493730459255 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4311 0.2371292346077769 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4205 0.23129863872087728 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4152 0.22838334077742747 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3478 0.19130955183619766 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3235 0.17794318579358812 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3176 0.17469785412069114 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3170 0.174367820391244 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3155 0.1735427360676261 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2948 0.16215657240169945 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2926 0.1609464487270599 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2923 0.16078143186233634 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2802 0.1541257516518188 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2556 0.1405943687444857 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2504 0.1377340764226104 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2494 0.13718402020686513 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2491 0.13701900334214157 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2255 0.12403767665055368 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2080 0.11441169287501182 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2071 0.1139166422808411 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2057 0.11314656357879775 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1992 0.10957119817645362 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1861 0.10236546175019087 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1836 0.10099032121082775 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.1001124314904983E-4 0.0 16-17 0.0 0.0 0.0 3.850393510216744E-4 0.0 18-19 0.0 0.0 0.0 6.325646481070365E-4 0.0 20-21 0.0 0.0 0.0 0.0011551180530650232 0.0 22-23 0.0 0.0 0.0 0.0025027557816408837 0.0 24-25 0.0 0.0 0.0 0.004262935672025681 0.0 26-27 0.0 0.0 0.0 0.005940607130048691 0.0 28-29 0.0 0.0 0.0 0.010946118693330458 0.0 30-31 0.0 0.0 0.0 0.02392744538491834 0.0 32-33 0.0 0.0 0.0 0.050027612822030415 0.0 34-35 0.0 0.0 0.0 0.08751394392506914 0.0 36-37 0.0 0.0 0.0 0.14282209641825394 0.0 38-39 0.0 0.0 0.0 0.22002248629809967 0.0 40-41 0.0 0.0 0.0 0.3070138768182108 0.0 42-43 0.0 0.0 0.0 0.39579295003949405 0.0 44-45 0.0 0.0 0.0 0.4935929451989993 0.0 46-47 0.0 0.0 0.0 0.6094897898565234 0.0 48-49 0.0 0.0 0.0 0.7277793790525392 0.0 50-51 0.0 0.0 0.0 0.8517345472707311 0.0 52-53 2.7502810787262458E-5 0.0 0.0 0.9684014706302984 0.0 54-55 5.5005621574524916E-5 0.0 0.0 1.0968120941960269 0.0 56-57 5.5005621574524916E-5 0.0 0.0 1.2270104004629272 0.0 58-59 5.5005621574524916E-5 0.0 0.0 1.3234902607046442 0.0 60-61 5.5005621574524916E-5 0.0 0.0 1.4102066231168826 0.0 62-63 8.250843236178737E-5 0.0 0.0 1.4826215239197447 0.0 64-65 1.6501686472357476E-4 0.0 0.0 1.5588868182328235 0.0 66-67 1.6501686472357476E-4 0.0 0.0 1.6271212917960214 0.0 68-69 1.6501686472357476E-4 0.0 0.0 1.7123800052365352 0.0 70-71 1.6501686472357476E-4 5.5005621574524916E-5 0.0 1.8361701565900035 0.0 72-73 1.6501686472357476E-4 5.5005621574524916E-5 2.7502810787262458E-5 1.9981892149377667 0.0 74-75 1.6501686472357476E-4 5.5005621574524916E-5 5.5005621574524916E-5 2.221539541341125 0.0 76-77 1.6501686472357476E-4 5.5005621574524916E-5 5.5005621574524916E-5 2.485896558628292 0.0 78-79 1.6501686472357476E-4 5.5005621574524916E-5 5.5005621574524916E-5 2.850391309991881 0.0 80-81 1.6501686472357476E-4 5.5005621574524916E-5 5.5005621574524916E-5 3.281882908433242 0.0 82-83 1.6501686472357476E-4 5.5005621574524916E-5 5.5005621574524916E-5 3.689227039003386 0.0 84-85 1.6501686472357476E-4 5.5005621574524916E-5 5.5005621574524916E-5 4.078144286346065 0.0 86-87 1.6501686472357476E-4 5.5005621574524916E-5 5.5005621574524916E-5 4.430372784098536 0.0 88-89 1.6501686472357476E-4 5.5005621574524916E-5 5.5005621574524916E-5 4.764586940785348 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11510 0.0 26.317183 1 TACATGG 11545 0.0 25.98289 2 ACATGGG 11720 0.0 24.746399 3 ATGGGAG 2840 0.0 22.915283 5 GAGTACT 10300 0.0 22.557938 12-13 CATGGGA 8560 0.0 22.532743 4 AGTACTT 10565 0.0 22.215162 12-13 GCTTACG 475 0.0 22.15268 60-61 CGCATAG 860 0.0 21.778362 66-67 TACCGGG 135 3.9826927E-4 20.962435 2 CATGGGG 3425 0.0 20.791262 4 ACGCATA 1095 0.0 20.436506 64-65 GTACTTT 11485 0.0 20.394594 14-15 TACGCGG 100 1.0465805E-5 19.459108 64-65 TACTTTT 12220 0.0 19.322182 14-15 AACGCAT 1285 0.0 19.118383 64-65 ACTTTTT 12820 0.0 18.620062 16-17 TTACGCT 275 0.0 18.523592 62-63 GTATAGG 485 0.0 18.48381 1 ACGGCGT 200 1.6370905E-11 18.242914 68-69 >>END_MODULE