FastQCFastQC Report
Fri 27 May 2016
SRR939579_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939579_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741503
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73100.26664205729484886No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54710.1995620650424238No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50730.1850444810747973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33630.12266993689228134No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29660.10818882926628204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG85500.025.359382
GTACATG88000.025.177171
ACATGGG86350.024.0108573
GTATCAA161600.022.517841
TATCAAC182300.019.5973282
ATCAACG183350.019.5886063
CAACGCA183900.019.5554645
TCAACGC184200.019.5243284
CATGGGG47200.019.299934
AGTACTT73750.019.265312-13
GAGTACT69150.019.24339512-13
AACGCAG187650.019.2373546
AGAGTAC119100.018.9999110-11
CATGGGA47700.018.4013564
GTACTTT75150.017.86595314-15
ACGCAGA203050.017.3350877
CGCAGAG202250.017.2870148
GGTATCA128350.016.3749731
TAGCCCT18000.016.0788084
GCAGAGT204450.015.778979
Produced by FastQC (version 0.11.5)