FastQCFastQC Report
Fri 27 May 2016
SRR939579_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939579_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741503
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79240.28903853105395105No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62560.2281959932197776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62180.22680989223794393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41990.15316415849262247No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33120.12080964346929404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG88450.024.5249441
TACATGG86350.024.1870442
GTATCAA147600.022.7942161
ACATGGG87600.022.4974043
GGTATCA111800.020.412911
GAGTACT68350.020.23870712-13
TCAACGC166700.019.9201054
ATCAACG167800.019.789523
CAACGCA168200.019.7704225
AACGCAG167750.019.74076
CATGGGG44350.019.4079634
TATCAAC174750.019.058042
AGAGTAC114350.018.89546810-11
GTACTTT74500.018.50477814-15
CATGGGA48650.017.7892444
ACGCAGA184600.017.684357
CGCAGAG181200.017.6795048
AGTACTT75250.017.5402512-13
GCAGAGT190450.016.277929
GTGTAGC18600.015.9379541
Produced by FastQC (version 0.11.5)