##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939579_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2741503 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56431526793879 31.0 31.0 34.0 30.0 34.0 2 31.782335820898243 31.0 31.0 34.0 30.0 34.0 3 31.82794656799573 31.0 31.0 34.0 30.0 34.0 4 35.603960309363146 37.0 35.0 37.0 33.0 37.0 5 35.33898339706358 37.0 35.0 37.0 33.0 37.0 6 35.359135481522365 37.0 35.0 37.0 33.0 37.0 7 35.295556853302735 37.0 35.0 37.0 32.0 37.0 8 35.255641886950336 37.0 35.0 37.0 32.0 37.0 9 36.87865269525512 39.0 37.0 39.0 33.0 39.0 10-11 36.764562176295264 39.0 36.0 39.0 32.0 39.0 12-13 36.70363829621926 39.0 35.0 39.0 32.0 39.0 14-15 37.778329441915616 40.0 37.0 41.0 32.0 41.0 16-17 37.74423154014422 39.5 37.0 41.0 32.0 41.0 18-19 37.71925527712354 39.5 37.0 41.0 32.0 41.0 20-21 37.65639650950591 39.0 37.0 41.0 32.0 41.0 22-23 37.52014752491608 39.0 36.0 41.0 32.0 41.0 24-25 37.39590235721062 39.0 36.0 41.0 31.5 41.0 26-27 37.13676530720557 39.0 36.0 40.5 31.0 41.0 28-29 36.95092254139426 39.0 36.0 40.0 31.0 41.0 30-31 36.73588283507259 39.0 35.5 40.0 30.0 41.0 32-33 36.585797644576715 38.0 35.0 40.0 30.0 41.0 34-35 36.5850150446671 38.0 35.0 40.0 30.0 41.0 36-37 36.49593726506956 38.5 35.0 40.0 30.0 41.0 38-39 36.53550297045088 39.0 35.0 40.0 30.0 41.0 40-41 36.458653154857025 39.0 35.0 40.0 30.0 41.0 42-43 36.289547923164776 39.0 35.0 40.0 30.0 41.0 44-45 36.09963458000958 38.0 35.0 40.0 29.5 41.0 46-47 35.82931516033358 38.0 35.0 40.0 28.5 41.0 48-49 35.5527238160965 38.0 34.5 40.0 27.5 41.0 50-51 35.31342022970611 38.0 34.0 40.0 27.0 41.0 52-53 35.08913650650756 38.0 34.0 40.0 26.5 41.0 54-55 34.80635968663904 38.0 34.0 40.0 26.0 41.0 56-57 32.7486201911871 37.0 30.5 39.5 16.5 41.0 58-59 27.712081839779128 32.5 18.0 38.5 7.5 40.0 60-61 28.500683201878676 33.0 20.5 38.0 9.5 40.0 62-63 31.564579174270463 35.0 30.0 38.5 15.5 40.0 64-65 31.799501040122884 35.5 31.0 39.0 10.5 40.0 66-67 25.04284912327289 28.0 18.0 35.5 2.0 39.0 68-69 17.452947707881407 13.0 7.5 28.0 2.0 35.0 70-71 16.310958806173108 12.5 7.5 26.5 2.0 33.5 72-73 17.431793800699836 13.5 7.0 28.5 2.0 34.0 74-75 18.47578518061078 17.5 9.0 29.5 2.0 34.5 76-77 19.713368725111735 21.5 8.0 30.0 2.0 34.5 78-79 22.85935762244287 27.5 10.5 33.5 2.0 35.5 80-81 26.508764535366183 31.5 23.5 34.5 2.0 36.0 82-83 27.808747792725377 33.5 27.5 35.0 2.0 36.0 84-85 27.85906635885498 34.0 28.0 35.0 2.0 36.0 86-87 27.624442869477072 34.0 27.0 35.0 2.0 36.0 88-89 27.202007438985113 33.5 26.5 35.0 2.0 35.5 90-91 25.87143694535443 32.5 21.5 35.0 2.0 35.0 92-93 23.388734026554047 31.5 2.0 34.5 2.0 35.0 94-95 15.938749839048143 7.0 2.0 32.0 2.0 35.0 96-97 13.331753239007945 2.0 2.0 30.0 2.0 34.0 98-99 12.982749426135955 2.0 2.0 29.5 2.0 34.0 100-101 12.695866282108756 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3509035904099811 1101 2 0.3100740749021007 1101 3 0.33998399069682606 1101 4 0.18327760472368482 1101 5 0.4448000784306032 1101 6 0.334046037300439 1101 7 0.30093429502584 1101 8 0.3651367711336988 1101 9 0.28133200788523993 1101 10-11 0.4472093880179244 1101 12-13 0.5038070051269798 1101 14-15 0.6111608121150383 1101 16-17 0.44015670715129573 1101 18-19 0.42573026049821294 1101 20-21 0.5685328665249401 1101 22-23 0.6073886790285385 1101 24-25 0.3371076874727308 1101 26-27 0.43027310397231133 1101 28-29 0.5209414113493551 1101 30-31 0.349321362949901 1101 32-33 0.4614402133749067 1101 34-35 0.33234005096582564 1101 36-37 0.10856626671571235 1101 38-39 0.34478240783425207 1101 40-41 0.37363429416607374 1101 42-43 0.30368441495721044 1101 44-45 0.2123598620579159 1101 46-47 0.31784911605193145 1101 48-49 0.3260569626023653 1101 50-51 0.14964653950328 1101 52-53 0.10737583191953348 1101 54-55 -0.08406477176582428 1101 56-57 -2.619876245910106 1101 58-59 -10.496411393872965 1101 60-61 -10.62258338339874 1101 62-63 -4.346275974228231 1101 64-65 -5.837072523978765 1101 66-67 -10.689231004765386 1101 68-69 -5.432570600141096 1101 70-71 -5.048686122272695 1101 72-73 -5.037494949381102 1101 74-75 -6.979157489828733 1101 76-77 -8.719931267314037 1101 78-79 -11.41739299393888 1101 80-81 -8.481050936545028 1101 82-83 -4.793877216433895 1101 84-85 -3.9711922676298137 1101 86-87 -3.7502654526893657 1101 88-89 -3.8179698644598083 1101 90-91 -10.369635084121441 1101 92-93 -17.46953896686919 1101 94-95 -11.348941499130818 1101 96-97 -9.315206417916343 1101 98-99 -9.433143409829725 1101 100-101 -9.361214121777364 1102 1 0.369924960530426 1102 2 0.4595970201880242 1102 3 0.4671878547595618 1102 4 0.37725657891928677 1102 5 0.3690112889750452 1102 6 0.37452762926339744 1102 7 0.4884697322267755 1102 8 0.4777483922830754 1102 9 0.5379211551720644 1102 10-11 0.6065522592276267 1102 12-13 0.4458135632214635 1102 14-15 0.6191446002995278 1102 16-17 0.6326473993552781 1102 18-19 0.5753011525985698 1102 20-21 0.6457203192619332 1102 22-23 0.7406784097218946 1102 24-25 0.6290532662293913 1102 26-27 0.6012754176474502 1102 28-29 0.7656775590641587 1102 30-31 0.7662441750144708 1102 32-33 0.6436884684648234 1102 34-35 0.41060276732984136 1102 36-37 0.48798598154478867 1102 38-39 0.4418578130491895 1102 40-41 0.38486945559238706 1102 42-43 0.4907563126535095 1102 44-45 0.4528969629167747 1102 46-47 0.3154540278652007 1102 48-49 0.4255419365855886 1102 50-51 0.3285888778275847 1102 52-53 0.19335669824446455 1102 54-55 0.2755327967836365 1102 56-57 -2.713318955902672 1102 58-59 -10.14222554043576 1102 60-61 -10.45870326330645 1102 62-63 -4.231896756758854 1102 64-65 -5.352646711215904 1102 66-67 -6.94820751726213 1102 68-69 -4.16049125299007 1102 70-71 -4.364747500630983 1102 72-73 -4.572578180545889 1102 74-75 -5.256519031334907 1102 76-77 -7.6444023238494445 1102 78-79 -9.957566632201083 1102 80-81 -5.6609491541463335 1102 82-83 -3.228436089651577 1102 84-85 -2.8247438715955653 1102 86-87 -2.746608140478372 1102 88-89 -2.868349899277561 1102 90-91 -8.803109522677115 1102 92-93 -13.299656323937807 1102 94-95 -10.452654642028413 1102 96-97 -9.095371882694742 1102 98-99 -9.303791753733883 1102 100-101 -9.259956402822926 1103 1 0.3180130703864066 1103 2 0.2873738485526154 1103 3 0.28136522222500915 1103 4 0.24495045261715376 1103 5 0.26495512218810546 1103 6 0.20822919395438078 1103 7 0.21045010698545497 1103 8 0.24348265227684607 1103 9 0.40199326215044806 1103 10-11 0.4111918549318432 1103 12-13 0.3946832349923284 1103 14-15 0.5766742139065997 1103 16-17 0.4966342083990938 1103 18-19 0.4338351624447796 1103 20-21 0.3957358788589147 1103 22-23 0.5359208297354954 1103 24-25 0.5575185228060278 1103 26-27 0.4161595714105957 1103 28-29 0.4388679630092085 1103 30-31 0.4601537681590031 1103 32-33 0.4462438244363156 1103 34-35 0.48179034890578976 1103 36-37 0.24629439589126179 1103 38-39 0.4330574990214515 1103 40-41 0.468432849569588 1103 42-43 0.34245462214247624 1103 44-45 0.32460849619751997 1103 46-47 0.2509564759830809 1103 48-49 0.11673378678958102 1103 50-51 0.27086225061836444 1103 52-53 0.3163883345991465 1103 54-55 0.3654742822512702 1103 56-57 -2.609177565807535 1103 58-59 -10.413383495345016 1103 60-61 -10.49794382064599 1103 62-63 -4.212326291187356 1103 64-65 -5.4340451197340585 1103 66-67 -6.715726466761723 1103 68-69 -4.415044095545603 1103 70-71 -4.260726781367158 1103 72-73 -4.601512687702185 1103 74-75 -5.15880916520714 1103 76-77 -6.693316398307154 1103 78-79 -9.34223561576761 1103 80-81 -5.203865358440485 1103 82-83 -2.8877605419500334 1103 84-85 -2.4082618181168165 1103 86-87 -2.2531888425482833 1103 88-89 -2.22725263799051 1103 90-91 -5.151942403650306 1103 92-93 -10.335850931585966 1103 94-95 -8.718824122979662 1103 96-97 -8.19250705118988 1103 98-99 -8.486649704687945 1103 100-101 -8.496712872369027 1104 1 0.23708204231592944 1104 2 0.29973888339496213 1104 3 0.16866228655458926 1104 4 0.1744068654393942 1104 5 0.2175122919621444 1104 6 0.20459086154216521 1104 7 0.23136801295793674 1104 8 0.210459406763583 1104 9 0.2678374407629178 1104 10-11 0.3062606630516598 1104 12-13 0.4091001596531001 1104 14-15 0.5048283766589705 1104 16-17 0.4480263372930864 1104 18-19 0.42273173455340185 1104 20-21 0.389820480140024 1104 22-23 0.42968460401016983 1104 24-25 0.43658000452423096 1104 26-27 0.4476124583649437 1104 28-29 0.5002218131374292 1104 30-31 0.4340543284896583 1104 32-33 0.5160270697961593 1104 34-35 0.525035555337567 1104 36-37 0.4357380973770191 1104 38-39 0.2596965333161947 1104 40-41 0.18784692302586592 1104 42-43 0.1800714432715509 1104 44-45 0.3418642530138385 1104 46-47 0.23054855763950144 1104 48-49 0.2867268174535482 1104 50-51 0.2891632222549134 1104 52-53 0.18944997641535366 1104 54-55 0.1522546036573118 1104 56-57 -2.7663850878404936 1104 58-59 -10.349981776407091 1104 60-61 -10.293997516082914 1104 62-63 -4.123775266009535 1104 64-65 -5.396808970593483 1104 66-67 -6.47125569398942 1104 68-69 -4.087885361339353 1104 70-71 -4.147247357982284 1104 72-73 -4.270995854460592 1104 74-75 -4.923583928713059 1104 76-77 -6.604331970251103 1104 78-79 -9.430541854306963 1104 80-81 -5.0063943625949925 1104 82-83 -2.579295353554638 1104 84-85 -2.0214073740546787 1104 86-87 -1.9580244086549676 1104 88-89 -2.0242859748747044 1104 90-91 -4.549996779813071 1104 92-93 -7.263968286643138 1104 94-95 -7.705835708062535 1104 96-97 -7.992304305744105 1104 98-99 -8.296182316244026 1104 100-101 -8.278430558212936 1105 1 0.2433227835343139 1105 2 0.2748329866950847 1105 3 0.21152571830311118 1105 4 0.18247652948105042 1105 5 0.18793926532165273 1105 6 0.14153177567213504 1105 7 0.2685095503770327 1105 8 0.19947814443801093 1105 9 0.27250394768853425 1105 10-11 0.37168552376730446 1105 12-13 0.31127353500754396 1105 14-15 0.39198746441051213 1105 16-17 0.44586217010028406 1105 18-19 0.46233785699652685 1105 20-21 0.4335519586829548 1105 22-23 0.4851181109279352 1105 24-25 0.4745244213691109 1105 26-27 0.48717447134633574 1105 28-29 0.5602534822357228 1105 30-31 0.566432368008499 1105 32-33 0.5537476996543589 1105 34-35 0.4907208238613947 1105 36-37 0.34672707262824787 1105 38-39 0.4108743618572319 1105 40-41 0.36267801431513647 1105 42-43 0.46964239651814665 1105 44-45 0.41020015129991094 1105 46-47 0.5508938350425296 1105 48-49 0.4286818434962001 1105 50-51 0.3994748805227175 1105 52-53 0.566354611136056 1105 54-55 0.5029843224320771 1105 56-57 1.7802689540502854 1105 58-59 -8.333179124257171 1105 60-61 -9.84592064500378 1105 62-63 -3.47668323211602 1105 64-65 -4.509848437721281 1105 66-67 -6.355248370323533 1105 68-69 -4.032315289534385 1105 70-71 -4.015350597548121 1105 72-73 -2.4118449042817325 1105 74-75 -4.754115812813829 1105 76-77 -6.444426449229269 1105 78-79 -9.09003593622521 1105 80-81 -4.507414861159003 1105 82-83 -2.315969280838072 1105 84-85 -1.8310118106192306 1105 86-87 -1.7238977404855866 1105 88-89 -1.8061946535857487 1105 90-91 -4.3834926577589535 1105 92-93 -6.596277944035979 1105 94-95 -8.155744118627924 1105 96-97 -8.3056170521699 1105 98-99 -8.43933490171265 1105 100-101 -8.357629844387272 1106 1 0.2987258054875319 1106 2 0.2565687846562348 1106 3 0.2431430833746866 1106 4 0.11702928404024959 1106 5 0.12879448517415426 1106 6 0.25992518264472864 1106 7 0.17217648532669472 1106 8 0.2620544836653238 1106 9 0.19513474608027082 1106 10-11 0.24520913540278855 1106 12-13 0.282334654364476 1106 14-15 0.27055203514230897 1106 16-17 0.3877633062753105 1106 18-19 0.43727400693384766 1106 20-21 0.38495119209481743 1106 22-23 0.31738777318636124 1106 24-25 0.41173696199521004 1106 26-27 0.4094310790195479 1106 28-29 0.4602528564738151 1106 30-31 0.38082393822092087 1106 32-33 0.38315822598269733 1106 34-35 0.5844365652596224 1106 36-37 0.4159539199593283 1106 38-39 0.4377553052916383 1106 40-41 0.3751429679238285 1106 42-43 0.2813860254425222 1106 44-45 0.43993516497280893 1106 46-47 0.4882581229299561 1106 48-49 0.6800702899647959 1106 50-51 0.563243549458619 1106 52-53 0.6600355631523342 1106 54-55 0.7287525122131484 1106 56-57 1.8789372284284056 1106 58-59 -8.834446813577713 1106 60-61 -9.932916299534341 1106 62-63 -3.7508075453795797 1106 64-65 -5.457513809458117 1106 66-67 -7.063974083684652 1106 68-69 -4.17822702313622 1106 70-71 -4.1482432802758105 1106 72-73 -4.422628420759681 1106 74-75 -5.115852901036424 1106 76-77 -6.833971055474553 1106 78-79 -9.505361113397772 1106 80-81 -5.0435967868310065 1106 82-83 -2.5212030937930727 1106 84-85 -2.041580988746528 1106 86-87 -1.8856604700470854 1106 88-89 -1.8376792677394675 1106 90-91 -4.492488014903618 1106 92-93 -6.98371954331653 1106 94-95 -7.848822208647647 1106 96-97 -7.47803218619171 1106 98-99 -6.984414714663478 1106 100-101 -7.631687111732723 1107 1 0.05476268961362862 1107 2 0.028939438979566745 1107 3 -0.01801518099435029 1107 4 0.08149337706910131 1107 5 -0.024035327777085058 1107 6 -0.06416290254836099 1107 7 0.07802199064547466 1107 8 0.02622496710231559 1107 9 0.1847620263014491 1107 10-11 0.16043689123445404 1107 12-13 0.1421069853652952 1107 14-15 0.24533790622269436 1107 16-17 0.10443410230926276 1107 18-19 0.19692703009381773 1107 20-21 0.25421778752025403 1107 22-23 0.13121963885765808 1107 24-25 0.28436817167599315 1107 26-27 0.22967494517247644 1107 28-29 0.2637502086617687 1107 30-31 0.25847753910638005 1107 32-33 0.24409034960437026 1107 34-35 0.17665136684159677 1107 36-37 0.37824228023774964 1107 38-39 0.07780496768691592 1107 40-41 0.0632578866717779 1107 42-43 0.13066957056995676 1107 44-45 -0.12936193760067027 1107 46-47 -0.02960462269021491 1107 48-49 -0.21773828954829355 1107 50-51 -0.06329005703043578 1107 52-53 -0.06526348794221803 1107 54-55 -0.07911121171974145 1107 56-57 1.0875796371848239 1107 58-59 -8.92282304023135 1107 60-61 -10.397441320026807 1107 62-63 -3.982559842388376 1107 64-65 -5.634497671350463 1107 66-67 -7.236549460074553 1107 68-69 -3.8961959648913 1107 70-71 -3.89947543330476 1107 72-73 -2.303286425789018 1107 74-75 -4.627306251149653 1107 76-77 -6.619197404619889 1107 78-79 -7.288671648378532 1107 80-81 -2.255933049699582 1107 82-83 -1.0899666107173012 1107 84-85 -0.8196424997244698 1107 86-87 -0.8251577620311714 1107 88-89 -0.5754393599903054 1107 90-91 0.005310781482297244 1107 92-93 -3.0736769896077405 1107 94-95 -6.1152325361566415 1107 96-97 -6.55713513710704 1107 98-99 -6.126801328798418 1107 100-101 -6.894652360212575 1108 1 -0.1539679589092522 1108 2 -0.2310403209193801 1108 3 -0.160467078684583 1108 4 -0.10103720932006865 1108 5 -0.152832154293435 1108 6 -0.3128221532867812 1108 7 -0.2009162460260967 1108 8 -0.16799254004865816 1108 9 -0.14909690126707176 1108 10-11 -0.14073641888737143 1108 12-13 -0.17021324904483492 1108 14-15 -0.26927226075464006 1108 16-17 -0.22647988822327392 1108 18-19 -0.19805936999500062 1108 20-21 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2.620300559454531 2312 76-77 4.033935840579627 2312 78-79 5.970943086868459 2312 80-81 2.7879107119801567 2312 82-83 1.2800698485604336 2312 84-85 0.8813471323707951 2312 86-87 0.6914014433582487 2312 88-89 0.7785875353185574 2312 90-91 2.1335512720959535 2312 92-93 4.46559617928725 2312 94-95 10.896883512771504 2312 96-97 13.170048210226557 2312 98-99 12.941493785972911 2312 100-101 11.933707333089133 2313 1 -0.43962846191989513 2313 2 -0.35880746079474335 2313 3 -0.41446064216043155 2313 4 -0.34040741386044004 2313 5 -0.5163644757210193 2313 6 -0.3386979611148533 2313 7 -0.3367246910178636 2313 8 -0.37634989764115545 2313 9 -0.40490642191290505 2313 10-11 -0.5048923824005556 2313 12-13 -0.44689401785276317 2313 14-15 -0.5217915454130448 2313 16-17 -0.5341086483658657 2313 18-19 -0.5794431989596802 2313 20-21 -0.7212723960996072 2313 22-23 -0.6320006241139353 2313 24-25 -0.7450553222030365 2313 26-27 -0.716248031617198 2313 28-29 -0.6890906290137622 2313 30-31 -0.7620949005388056 2313 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 14.0 4 34.0 5 95.0 6 203.0 7 533.0 8 1186.0 9 2100.0 10 3378.0 11 5458.0 12 8113.0 13 11582.0 14 14833.0 15 20438.0 16 28171.0 17 37979.0 18 43492.0 19 45011.0 20 46986.0 21 47467.0 22 47480.0 23 40190.0 24 41285.0 25 47384.0 26 67677.0 27 100691.0 28 142243.0 29 193062.0 30 240033.0 31 265274.0 32 269083.0 33 245775.0 34 218931.0 35 197338.0 36 161219.0 37 104258.0 38 39617.0 39 2884.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.28184540303841 19.736081198375828 14.556543370050749 23.425530028535015 2 15.55862220918712 21.187159743714332 39.40333766701832 23.850880380080223 3 19.312864969128203 25.01660587875465 29.97487870686803 25.695650445249115 4 12.863272445808011 17.35248876255105 37.68480282531152 32.09943596632942 5 13.403031512845228 37.8095334456657 34.804283435529484 13.983151605959582 6 31.534672768915446 37.26134897536132 17.44944287859616 13.754535377127072 7 27.914541767782126 32.591100575122475 21.44932907241028 18.045028584685117 8 25.652543915420345 35.57002228372223 19.953105330985682 18.824328469871745 9 25.777502340869223 16.792649871256753 20.418325276317407 37.01152251155661 10-11 24.612630371004517 26.72694503708367 28.055066873900923 20.605357718010886 12-13 25.725578268562906 24.03451683255499 28.145984155406722 22.093920743475383 14-15 22.54964006733577 25.578564172337252 26.285571505953893 25.586224254373086 16-17 22.082230075983865 28.668033556775242 26.970515808299318 22.279220558941574 18-19 21.929474675793063 27.714998828195174 28.6903963983188 21.66513009769296 20-21 22.85080483223983 26.719631530587417 28.508431323985416 21.92113231318733 22-23 22.379198645118567 26.669413343707237 28.503449487131927 22.447938524042268 24-25 22.489387204532406 26.842311258572852 28.54548346125591 22.12281807563883 26-27 22.30968069753271 26.829884389574072 28.683273320380533 22.17716159251269 28-29 22.113008310689867 27.231817652893092 28.63689803309174 22.018276003325298 30-31 22.409984289420503 26.826183861387925 28.450704841933838 22.31312700725773 32-33 22.263879427559964 26.89640141204065 28.48065192432031 22.35906723607908 34-35 22.727081217223283 26.832035290377572 28.38629437356242 22.054589118836727 36-37 22.58341495832333 26.59324829728597 28.579579116295754 22.24375762809494 38-39 22.273891741453685 27.03208423371086 28.61971850528688 22.074305519548567 40-41 22.49986898956536 26.85609832118573 28.566501170512044 22.077531518736865 42-43 22.195624002452636 26.927264702987664 28.21027883029429 22.66683246426541 44-45 22.485790848298407 26.86292897688265 28.124351805595726 22.526928369223217 46-47 22.73353595758949 27.076428489138387 27.856562913480467 22.33347263979166 48-49 22.55109310339413 27.10328969878174 27.91237718539432 22.433240012429803 50-51 22.271495302787837 27.271928919691412 28.054794570568642 22.401781206952112 52-53 22.627939617111636 27.331107949956074 27.55406004081188 22.48689239212041 54-55 22.512688136403103 27.082151154503148 27.69754424991563 22.70761645917812 56-57 21.656983888610515 27.261599342074856 28.62550281606341 22.45591395325122 58-59 21.23282034705531 26.66601301568614 29.85528612119801 22.245880516060538 60-61 22.31926047452078 27.097214348419495 28.37537393942016 22.208151237639555 62-63 22.388809133798926 27.107675937489113 28.06144791753492 22.44206701117704 64-65 21.952109382461167 27.28100928533303 28.217157259018045 22.549724073187758 66-67 21.858183752259524 26.812472569571007 28.82820926720771 22.501134410961757 68-69 21.925215558899648 26.290023813338887 29.547080701051776 22.237679926709692 70-71 21.911255765875477 26.31048339801323 29.44790411048111 22.330356725630185 72-73 21.148885971792268 26.395031925469887 29.936568762391985 22.519513340345856 74-75 21.600075059016827 26.89694817707301 28.97588933858523 22.527087425324936 76-77 20.033567800963034 27.22968132237375 29.633233566172716 23.103517310490503 78-79 20.92108136533587 27.268878984405408 28.90999903148342 22.900040618775304 80-81 22.06656033062104 27.262987921230064 27.712960558645616 22.95749118950328 82-83 22.437970398251903 27.200210396267583 27.52014549733567 22.84167370814485 84-85 22.271017523996008 27.26761062747447 27.39763078433761 23.063741064191912 86-87 22.18202516911852 27.255243444770727 27.592559459812886 22.970171926297862 88-89 22.312381770842073 27.243718580133923 27.256868325273263 23.187031323750737 90-91 21.681036484600213 27.246867950168053 27.86095413835763 23.2111414268741 92-93 20.78567568918607 27.424124208676346 28.581256233679365 23.208943868458217 94-95 19.859580080588337 27.18948870432234 29.356624337156735 23.59430687793259 96-97 19.802102747122895 27.308302695070257 29.108587339242604 23.781007218564245 98-99 20.14366403238196 27.377624376919528 28.512821158116854 23.96589043258166 100-101 20.52733349648046 27.218372797076462 28.269069753866965 23.985223952576114 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 282.0 1 207.0 2 147.5 3 187.5 4 248.5 5 296.0 6 344.5 7 525.0 8 743.0 9 945.5 10 1131.0 11 1300.0 12 1510.0 13 1714.0 14 2019.0 15 2293.5 16 2524.5 17 2842.5 18 3211.0 19 3589.5 20 4086.5 21 4848.0 22 5846.5 23 7180.0 24 8862.5 25 10973.0 26 13932.0 27 17685.5 28 21946.5 29 26548.0 30 31787.0 31 37929.5 32 44955.0 33 52341.5 34 59248.0 35 65967.0 36 72589.0 37 78130.5 38 82749.0 39 86877.5 40 90069.0 41 93151.0 42 96349.5 43 99178.0 44 101745.5 45 104327.5 46 107264.5 47 110473.5 48 112204.5 49 110767.0 50 106945.5 51 102585.0 52 98387.0 53 94018.0 54 88496.0 55 81407.5 56 73322.5 57 64742.5 58 56030.0 59 46983.0 60 37959.0 61 29550.5 62 22059.0 63 16185.5 64 11726.0 65 8229.0 66 5842.5 67 4108.5 68 2721.5 69 1732.5 70 1069.5 71 640.5 72 381.5 73 217.0 74 113.5 75 51.0 76 32.0 77 13.5 78 5.5 79 5.0 80 2.0 81 1.5 82 3.0 83 2.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024074385473953522 2 0.0068210758842868315 3 1.4590536650880922E-4 4 0.0 5 0.13839124013360554 6 0.0 7 0.0 8 0.001459053665088092 9 0.0 10-11 0.0 12-13 0.0 14-15 6.565741492896415E-4 16-17 0.0 18-19 2.006198789496127E-4 20-21 0.0 22-23 3.100489038312196E-4 24-25 3.6476341627202304E-5 26-27 4.1947792871282655E-4 28-29 0.003428776112957017 30-31 0.013003815790097621 32-33 0.0029363455009897856 34-35 0.004833115265604306 36-37 0.10434057522461221 38-39 0.10348338119637293 40-41 0.1156847174706721 42-43 0.11761796357691384 44-45 0.11406152026826161 46-47 0.10711277718827956 48-49 0.10665682291793954 50-51 0.1061643923059723 52-53 0.08570116465311181 54-55 0.07421111704054309 56-57 0.5601671783689458 58-59 1.5989404352284131 60-61 0.5273384709044637 62-63 0.004541304532586687 64-65 0.12283408042960377 66-67 1.9289783742713396 68-69 2.9093347700148424 70-71 2.8170131493563932 72-73 2.660730263654645 74-75 2.0286864541092973 76-77 1.4302738315442296 78-79 0.9487496457235319 80-81 0.052799504505375336 82-83 3.465252454584219E-4 84-85 1.823817081360115E-4 86-87 3.100489038312196E-4 88-89 1.823817081360115E-4 90-91 0.25358352699231046 92-93 0.396589024341757 94-95 2.0291971228920778 96-97 2.4543835990695615 98-99 1.4409796378118134 100-101 0.9543122878216802 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2741503.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.335778663395644 #Duplication Level Percentage of deduplicated Percentage of total 1 76.58193087309148 42.37720776408856 2 13.185055241385472 14.592105970039022 3 4.3630485131692875 7.242980604671794 4 1.9541152975913925 4.325299663610912 5 1.0387936875015706 2.874122878425975 6 0.6283264895379101 2.0861361332050907 7 0.434005322978588 1.681121574375508 8 0.2783505415826625 1.2322195167883612 9 0.23144931810785266 1.1526685414748983 >10 1.1559851319268724 11.867245494103011 >50 0.09906454897572654 3.765784925910251 >100 0.04717146102021814 4.581446227591987 >500 0.0016485202017987933 0.5713425636661473 >1k 8.572305048829569E-4 0.906018383900741 >5k 1.9782242420375925E-4 0.744299758147697 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7924 0.28903853105395105 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6256 0.2281959932197776 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6218 0.22680989223794393 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4199 0.15316415849262247 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3312 0.12080964346929404 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.6476341627202304E-5 0.0 0.0 0.0 0.0 6 3.6476341627202304E-5 0.0 0.0 0.0 3.6476341627202304E-5 7 3.6476341627202304E-5 0.0 0.0 0.0 3.6476341627202304E-5 8 3.6476341627202304E-5 0.0 0.0 0.0 3.6476341627202304E-5 9 3.6476341627202304E-5 0.0 0.0 3.6476341627202304E-5 7.295268325440461E-5 10-11 3.6476341627202304E-5 0.0 0.0 3.6476341627202304E-5 1.0942902488160692E-4 12-13 5.471451244080346E-5 0.0 0.0 5.471451244080346E-5 1.0942902488160692E-4 14-15 7.295268325440461E-5 0.0 0.0 7.295268325440461E-5 1.2766719569520807E-4 16-17 7.295268325440461E-5 0.0 0.0 1.2766719569520807E-4 1.4590536650880922E-4 18-19 7.295268325440461E-5 0.0 0.0 1.4590536650880922E-4 1.4590536650880922E-4 20-21 7.295268325440461E-5 0.0 0.0 2.9181073301761843E-4 2.0061987894961266E-4 22-23 7.295268325440461E-5 0.0 0.0 6.200978076624392E-4 2.1885804976321384E-4 24-25 1.4590536650880922E-4 0.0 0.0 7.477650033576472E-4 2.735725622040173E-4 26-27 1.4590536650880922E-4 0.0 0.0 0.0011125284196296702 2.9181073301761843E-4 28-29 1.4590536650880922E-4 0.0 0.0 0.00237096220576815 2.9181073301761843E-4 30-31 1.4590536650880922E-4 0.0 0.0 0.005963881856047577 2.9181073301761843E-4 32-33 1.4590536650880922E-4 0.0 0.0 0.015338301654238568 2.9181073301761843E-4 34-35 1.8238170813601154E-4 0.0 0.0 0.029472884034779462 2.9181073301761843E-4 36-37 2.1885804976321384E-4 0.0 0.0 0.050246160591471176 2.9181073301761843E-4 38-39 2.5533439139041614E-4 0.0 0.0 0.0823818175650364 2.9181073301761843E-4 40-41 2.5533439139041614E-4 0.0 0.0 0.11635952979077535 2.9181073301761843E-4 42-43 2.9181073301761843E-4 0.0 0.0 0.1538024944710985 3.2828707464482073E-4 44-45 3.465252454584219E-4 0.0 0.0 0.20016392467927263 3.64763416272023E-4 46-47 4.377160995264277E-4 0.0 0.0 0.25281752381813916 4.012397578992254E-4 48-49 4.7419244115363E-4 0.0 0.0 0.30034619695838377 4.012397578992254E-4 50-51 4.7419244115363E-4 0.0 0.0 0.3490056366890717 4.012397578992254E-4 52-53 4.7419244115363E-4 0.0 0.0 0.3977562672738275 4.012397578992254E-4 54-55 4.7419244115363E-4 0.0 0.0 0.45330973557205667 4.194779287128265E-4 56-57 4.7419244115363E-4 0.0 0.0 0.49919697333907714 4.377160995264277E-4 58-59 4.7419244115363E-4 0.0 0.0 0.5307854851882343 4.377160995264277E-4 60-61 4.924306119672311E-4 0.0 0.0 0.557322023722024 4.377160995264277E-4 62-63 5.471451244080346E-4 0.0 0.0 0.581597029074927 4.377160995264277E-4 64-65 5.471451244080346E-4 0.0 0.0 0.6035375485636894 4.377160995264277E-4 66-67 5.471451244080346E-4 0.0 0.0 0.6246391121950259 4.377160995264277E-4 68-69 5.471451244080346E-4 0.0 0.0 0.6539478526924829 4.377160995264277E-4 70-71 5.653832952216358E-4 0.0 0.0 0.7011847150997099 4.377160995264277E-4 72-73 5.836214660352369E-4 0.0 0.0 0.7661673177085708 4.377160995264277E-4 74-75 5.836214660352369E-4 0.0 0.0 0.8543123972507052 4.377160995264277E-4 76-77 5.836214660352369E-4 0.0 0.0 0.9950563614192653 4.377160995264277E-4 78-79 5.836214660352369E-4 0.0 0.0 1.2171425674164866 4.377160995264277E-4 80-81 6.930504909168438E-4 0.0 0.0 1.4884900727812445 4.377160995264277E-4 82-83 6.930504909168438E-4 0.0 0.0 1.7512291615219826 4.377160995264277E-4 84-85 6.930504909168438E-4 0.0 0.0 1.952961568891225 4.377160995264277E-4 86-87 7.11288661730445E-4 0.0 0.0 2.107584781048936 4.377160995264277E-4 88-89 7.29526832544046E-4 0.0 0.0 2.260785415883185 4.377160995264277E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8845 0.0 24.524944 1 TACATGG 8635 0.0 24.187044 2 GTATCAA 14760 0.0 22.794216 1 ACATGGG 8760 0.0 22.497404 3 GGTATCA 11180 0.0 20.41291 1 GAGTACT 6835 0.0 20.238707 12-13 TCAACGC 16670 0.0 19.920105 4 ATCAACG 16780 0.0 19.78952 3 CAACGCA 16820 0.0 19.770422 5 AACGCAG 16775 0.0 19.7407 6 CATGGGG 4435 0.0 19.407963 4 TATCAAC 17475 0.0 19.05804 2 AGAGTAC 11435 0.0 18.895468 10-11 GTACTTT 7450 0.0 18.504778 14-15 CATGGGA 4865 0.0 17.789244 4 ACGCAGA 18460 0.0 17.68435 7 CGCAGAG 18120 0.0 17.679504 8 AGTACTT 7525 0.0 17.54025 12-13 GCAGAGT 19045 0.0 16.27792 9 GTGTAGC 1860 0.0 15.937954 1 >>END_MODULE