FastQCFastQC Report
Fri 27 May 2016
SRR939578_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939578_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718370
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30370.11172136243410573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG73350.027.3751661
GTATCAA142100.023.8588221
TACATGG84300.023.5346952
ACATGGG83500.022.4024523
GAGTACT61700.019.92843612-13
GTCTAGA10900.019.4400141
GTCTTAG13550.019.3614481
TATCAAC170500.018.8395862
CATGGGA46950.018.712174
AGTACTT69700.018.6907612-13
GAGTACA70050.018.5816971
AGAGTAC111000.018.32706610-11
GTACTTT67050.018.3059914-15
ATCAACG177550.018.2777563
TCAACGC179200.018.1341514
CAACGCA179750.017.9875645
GTACTAA9053.6379788E-1217.8391931
AACGCAG182700.017.7746326
CATGGGG43850.017.7730164
GGTATCA117600.017.4177681
Produced by FastQC (version 0.11.5)