FastQCFastQC Report
Fri 27 May 2016
SRR939578_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939578_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718370
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77760.28605377487244193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61410.22590743717742617No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61370.22576029017389096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41450.152481082413358No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32170.11834297759319003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG86300.023.1975771
TACATGG84500.023.1235942
ACATGGG83550.022.0271283
GTATCAA157250.020.0573431
GAGTACT72100.019.36156712-13
GGTATCA124550.019.257841
AGAGTAC121300.018.4678710-11
AGTACTT75650.018.23479812-13
GTACTTT76700.018.16963414-15
AACGCAG176000.017.7922746
TCAACGC176400.017.7356344
CAACGCA177150.017.6605455
ATCAACG177200.017.6292553
TATCAAC183100.017.1155722
CATGGGA48200.017.034684
CATGGGG42750.016.1156754
CGCAGAG190300.016.0092058
ACGCAGA192650.015.9852787
AGTACAT64400.015.9032382
TTACGCG1104.9275014E-415.4082662-63
Produced by FastQC (version 0.11.5)