##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939578_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2718370 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.493039578865275 31.0 31.0 34.0 30.0 34.0 2 31.710053819016544 31.0 31.0 34.0 30.0 34.0 3 31.762409458609387 31.0 31.0 34.0 30.0 34.0 4 35.554671733428485 37.0 35.0 37.0 33.0 37.0 5 35.30545805022863 37.0 35.0 37.0 33.0 37.0 6 35.30928497592307 37.0 35.0 37.0 32.0 37.0 7 35.22440580200635 37.0 35.0 37.0 32.0 37.0 8 35.1870385561936 37.0 35.0 37.0 32.0 37.0 9 36.795211468637454 39.0 37.0 39.0 32.0 39.0 10-11 36.672754812626685 39.0 35.0 39.0 32.0 39.0 12-13 36.61170995854133 39.0 35.0 39.0 32.0 39.0 14-15 37.70255888639148 39.0 36.5 41.0 32.0 41.0 16-17 37.65024555156215 39.0 36.0 41.0 32.0 41.0 18-19 37.61319559147577 39.0 36.0 41.0 32.0 41.0 20-21 37.55187391709002 39.0 36.0 41.0 32.0 41.0 22-23 37.41942818674426 39.0 36.0 41.0 32.0 41.0 24-25 37.289050423599434 39.0 36.0 41.0 31.0 41.0 26-27 37.015242406294945 39.0 36.0 40.0 31.0 41.0 28-29 36.8247574833448 39.0 36.0 40.0 30.0 41.0 30-31 36.60002519892436 38.5 35.0 40.0 30.0 41.0 32-33 36.4547951529777 38.0 35.0 40.0 30.0 41.0 34-35 36.435805464303975 38.0 35.0 40.0 30.0 41.0 36-37 36.36896504155064 38.0 35.0 40.0 30.0 41.0 38-39 36.423709612745874 38.5 35.0 40.0 30.0 41.0 40-41 36.332570805298765 38.5 35.0 40.0 30.0 41.0 42-43 36.171437295143775 38.0 35.0 40.0 30.0 41.0 44-45 35.977685156913886 38.0 35.0 40.0 29.0 41.0 46-47 35.72234445642057 38.0 34.5 40.0 28.0 41.0 48-49 35.444396458171624 38.0 34.0 40.0 27.0 41.0 50-51 35.211775806825415 38.0 34.0 40.0 27.0 41.0 52-53 34.993614003980326 38.0 34.0 40.0 26.0 41.0 54-55 34.71744850774545 38.0 33.5 40.0 26.0 41.0 56-57 33.90038166254042 37.5 32.5 40.0 23.0 41.0 58-59 30.724988872007856 35.5 27.0 39.0 8.5 40.5 60-61 30.456909287550996 34.5 26.0 39.0 10.5 40.0 62-63 32.3226825266612 35.5 30.5 39.0 20.5 40.0 64-65 32.395343349139374 36.0 31.0 39.0 19.0 40.0 66-67 27.401744795594418 32.0 19.0 37.0 4.5 39.5 68-69 19.781957386227777 20.0 8.0 32.0 2.0 37.0 70-71 17.83076843843921 15.5 7.5 29.0 2.0 35.0 72-73 19.546233956378273 20.0 7.5 31.0 2.0 35.5 74-75 21.02707560045174 23.5 9.5 32.5 2.0 36.0 76-77 22.909522066532517 27.5 12.0 33.0 2.0 35.5 78-79 25.12162785051336 30.5 13.0 34.5 2.0 36.5 80-81 26.747629461773048 32.0 22.5 35.0 2.0 37.0 82-83 27.914811817375856 33.0 26.5 35.0 2.0 36.0 84-85 27.96750552720932 34.0 27.5 35.0 2.0 36.0 86-87 27.697296725611302 34.0 27.0 35.0 2.0 36.0 88-89 27.26171400508393 33.0 26.0 35.0 2.0 35.5 90-91 25.38970541169892 32.5 16.0 34.5 2.0 35.0 92-93 23.729766551278892 31.5 2.0 34.0 2.0 35.0 94-95 19.877528261421368 27.5 2.0 34.0 2.0 35.0 96-97 17.15573284725773 18.5 2.0 33.0 2.0 35.0 98-99 17.425458270949136 21.5 2.0 33.0 2.0 35.0 100-101 16.644677876815887 10.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.1670645747079611 1101 2 0.17082257785294885 1101 3 0.14613533359199948 1101 4 0.16038877725110012 1101 5 0.2708537824819146 1101 6 0.17562537506176312 1101 7 0.2112939823958584 1101 8 0.29652042675671453 1101 9 0.3139279783532274 1101 10-11 0.24010540923143964 1101 12-13 0.20945635459022327 1101 14-15 0.3318416009156593 1101 16-17 0.30671972435386863 1101 18-19 0.36750198490450003 1101 20-21 0.3726800773410517 1101 22-23 0.3050098092616622 1101 24-25 0.2829280012917579 1101 26-27 0.3095554290177702 1101 28-29 0.31591231946925546 1101 30-31 0.07177888000460086 1101 32-33 0.11519683526331193 1101 34-35 0.08037854854043758 1101 36-37 0.17659814822042819 1101 38-39 0.03997278573083207 1101 40-41 0.10094441605113502 1101 42-43 0.2042166787510311 1101 44-45 0.04137009305840422 1101 46-47 0.061006351726767605 1101 48-49 -0.025999774009413557 1101 50-51 -0.07457621045264062 1101 52-53 -0.07430899948395364 1101 54-55 -0.13106002226772517 1101 56-57 -3.531512872154231 1101 58-59 -10.164766159464257 1101 60-61 -12.052372973975796 1101 62-63 -4.2884535134405155 1101 64-65 -1.3896033483197563 1101 66-67 -2.5624451086993503 1101 68-69 -4.947919461383906 1101 70-71 -4.6378856761853555 1101 72-73 -3.6602360016401434 1101 74-75 -6.363193187946372 1101 76-77 -5.021111980171625 1101 78-79 0.9857858458009936 1101 80-81 1.6210920523158734 1101 82-83 0.16964441344130776 1101 84-85 0.04197134791328594 1101 86-87 -0.10474743158020061 1101 88-89 -0.2811760610523457 1101 90-91 1.1767409791934185 1101 92-93 0.5847353034526215 1101 94-95 -8.946907932250145 1101 96-97 -9.754903863116652 1101 98-99 -8.921241222087879 1101 100-101 -9.721492301905336 1102 1 0.2046034128587948 1102 2 0.24901362438935593 1102 3 0.28747436401335946 1102 4 0.11131953012896645 1102 5 0.2711643638029315 1102 6 0.32334513581379554 1102 7 0.31129646010882794 1102 8 0.2607221819685819 1102 9 0.230889905815701 1102 10-11 0.26956808000780086 1102 12-13 0.22044098533665846 1102 14-15 0.3526660970727349 1102 16-17 0.3484198194980479 1102 18-19 0.36525705306638656 1102 20-21 0.22268862545080736 1102 22-23 0.23709396230503899 1102 24-25 0.19994722586670832 1102 26-27 0.08323102949150751 1102 28-29 0.1902866969438648 1102 30-31 0.14071888961812817 1102 32-33 0.0657508518838057 1102 34-35 0.041669300724549885 1102 36-37 -0.15700211739039815 1102 38-39 0.021995429549683365 1102 40-41 0.018566530134947357 1102 42-43 -0.025561776543590042 1102 44-45 -0.03448725757267823 1102 46-47 0.010261675093090616 1102 48-49 0.1543696777718182 1102 50-51 0.15463472468579198 1102 52-53 0.1889901248922854 1102 54-55 -0.058489543722245685 1102 56-57 -3.7565553649316996 1102 58-59 -10.211563281848413 1102 60-61 -8.079303669866263 1102 62-63 -2.580859447067539 1102 64-65 -0.7292922219018863 1102 66-67 -1.8862979895362244 1102 68-69 -4.9045747842546845 1102 70-71 -4.8974383127197285 1102 72-73 -3.481821898993452 1102 74-75 -4.313700251906056 1102 76-77 -4.193541786563134 1102 78-79 1.798594630780105 1102 80-81 2.131286292624303 1102 82-83 0.6674458148357623 1102 84-85 0.48160348186835833 1102 86-87 0.4478121078625108 1102 88-89 0.3402200434873208 1102 90-91 1.7142621016965052 1102 92-93 1.1279107527858727 1102 94-95 -8.933645601911541 1102 96-97 -9.753971623611005 1102 98-99 -9.028228124897108 1102 100-101 -9.982007529929259 1103 1 0.1868357177589779 1103 2 0.1629199191644055 1103 3 0.2561748129578625 1103 4 0.18195808204414732 1103 5 0.14772134154674177 1103 6 0.25623175344680504 1103 7 0.22249810305466866 1103 8 0.3519543913486558 1103 9 0.2973040809995737 1103 10-11 0.23784274807543682 1103 12-13 0.2946713320746497 1103 14-15 0.4527308119452087 1103 16-17 0.4533331350009604 1103 18-19 0.4211291516431359 1103 20-21 0.3802480867174012 1103 22-23 0.3170127702141059 1103 24-25 0.40328732520762145 1103 26-27 0.4616814545559791 1103 28-29 0.3299753013951019 1103 30-31 0.4808440209582798 1103 32-33 0.36709338698744887 1103 34-35 0.22129366790383642 1103 36-37 0.23769030786511536 1103 38-39 0.25004896856964365 1103 40-41 0.2663308820310206 1103 42-43 0.12822105849318888 1103 44-45 0.10081769036887067 1103 46-47 0.08028030151395171 1103 48-49 0.1252432091501703 1103 50-51 -0.032005116750831064 1103 52-53 0.09639484358557127 1103 54-55 0.15165325883769754 1103 56-57 0.5642847344092132 1103 58-59 -8.879574314420772 1103 60-61 -7.574515963304034 1103 62-63 -2.3105045898700993 1103 64-65 -0.6102846758134319 1103 66-67 -1.9242087259962446 1103 68-69 -3.761036480691784 1103 70-71 -3.9735929120128795 1103 72-73 -2.926013560088112 1103 74-75 -3.820754411806714 1103 76-77 -0.702944069493693 1103 78-79 3.333263176008874 1103 80-81 2.832020125846192 1103 82-83 1.2576507054652417 1103 84-85 0.8046300364581 1103 86-87 0.7016565533682879 1103 88-89 0.5510899928616659 1103 90-91 1.9660750733270334 1103 92-93 1.687963050044445 1103 94-95 -7.371395625791568 1103 96-97 -8.82443967213522 1103 98-99 -8.023913481664708 1103 100-101 -9.749827999888186 1104 1 0.19872425713817066 1104 2 0.22851965802131247 1104 3 0.1865831457966074 1104 4 0.1548502418162343 1104 5 0.2753312887246153 1104 6 0.2060275870274353 1104 7 0.25063211694273235 1104 8 0.27769716540052514 1104 9 0.3078038139214101 1104 10-11 0.32712519807246565 1104 12-13 0.2910105806947385 1104 14-15 0.37619422223662724 1104 16-17 0.25741943092356934 1104 18-19 0.3256843181218656 1104 20-21 0.3625962972936989 1104 22-23 0.3117353056761516 1104 24-25 0.3163174753648974 1104 26-27 0.43138381077890386 1104 28-29 0.4029989229750228 1104 30-31 0.42955047237972366 1104 32-33 0.3549558714078884 1104 34-35 0.3276842548822927 1104 36-37 0.22127776112643716 1104 38-39 0.23507745690722714 1104 40-41 0.09125105533507849 1104 42-43 0.19995439578323015 1104 44-45 0.2419480245133201 1104 46-47 0.15323121848231835 1104 48-49 0.0882499157796417 1104 50-51 0.20412284336550357 1104 52-53 0.14455675230419018 1104 54-55 0.02018343867032968 1104 56-57 0.5350945747558242 1104 58-59 -8.415520364631465 1104 60-61 -7.745639472117613 1104 62-63 -2.1569209944044943 1104 64-65 -0.45925286383646124 1104 66-67 -1.7858533339848535 1104 68-69 -3.403658594763833 1104 70-71 -4.184498603745329 1104 72-73 -3.151820432899555 1104 74-75 -3.734620219866539 1104 76-77 -0.21284851486136702 1104 78-79 3.5131287466658883 1104 80-81 2.834848483612234 1104 82-83 1.3525032189675237 1104 84-85 0.9082302501206314 1104 86-87 0.790024705780894 1104 88-89 0.6573138043638309 1104 90-91 2.20195227672189 1104 92-93 1.8852996872335943 1104 94-95 -6.480320191047667 1104 96-97 -8.030436763950757 1104 98-99 -5.955631684525413 1104 100-101 -5.279714046537839 1105 1 0.20550270977211937 1105 2 0.24150454297262414 1105 3 0.16128295098411982 1105 4 0.20171954753163135 1105 5 0.26590424181218 1105 6 0.175702192545522 1105 7 0.25368982338366663 1105 8 0.16093966743687815 1105 9 0.1079008933976553 1105 10-11 0.21308634132886084 1105 12-13 0.20105058290487676 1105 14-15 0.2991247628813767 1105 16-17 0.41514797318076546 1105 18-19 0.311760958625392 1105 20-21 0.3037437957148157 1105 22-23 0.4241230983776063 1105 24-25 0.2573392576466489 1105 26-27 0.23438038210290557 1105 28-29 0.34793174861160736 1105 30-31 0.32872274210857455 1105 32-33 0.24819649007864086 1105 34-35 0.37440419841696837 1105 36-37 0.2019316157579354 1105 38-39 0.39661277417115315 1105 40-41 0.3596564387900969 1105 42-43 0.37984991355133246 1105 44-45 0.34652353351219034 1105 46-47 0.37384141705559415 1105 48-49 0.3761162883164886 1105 50-51 0.37915434666555115 1105 52-53 0.48174306350392015 1105 54-55 0.50344887380205 1105 56-57 0.9444982132755797 1105 58-59 -8.109966456113128 1105 60-61 -7.299863469047192 1105 62-63 -1.7805412508630383 1105 64-65 -0.05662518263975613 1105 66-67 -1.358020755086983 1105 68-69 -3.35872707619486 1105 70-71 -3.781632739182484 1105 72-73 -2.817252296950265 1105 74-75 -3.28175955386806 1105 76-77 0.17612512217902676 1105 78-79 4.081483510957039 1105 80-81 3.4012538753849597 1105 82-83 1.878421181463004 1105 84-85 1.5284908675199596 1105 86-87 1.3866666708501079 1105 88-89 1.2886639992654345 1105 90-91 2.5951969354198035 1105 92-93 2.3732037270825224 1105 94-95 -6.155636040965412 1105 96-97 -7.488832861284518 1105 98-99 -5.27680101097565 1105 100-101 -4.810065701196317 1106 1 0.053481322909295415 1106 2 0.10837373711168397 1106 3 0.06027105891178053 1106 4 0.06167596124102204 1106 5 0.09124820991027605 1106 6 0.09460794636870418 1106 7 0.130327612269582 1106 8 0.11262804136119797 1106 9 0.046280706299675956 1106 10-11 0.13481180078495925 1106 12-13 0.0013043958683454093 1106 14-15 0.11680454317796318 1106 16-17 0.2488427321248139 1106 18-19 0.14599746739866504 1106 20-21 0.09220650913417927 1106 22-23 0.16996070566792554 1106 24-25 0.042006852697078045 1106 26-27 0.15410892371021845 1106 28-29 0.24866341577227757 1106 30-31 0.30793794974726296 1106 32-33 0.2154046634586706 1106 34-35 0.36449834284381666 1106 36-37 0.3565901920068697 1106 38-39 0.3210845860224296 1106 40-41 0.23553002682994162 1106 42-43 0.16379366100384374 1106 44-45 0.14683174863242243 1106 46-47 0.26770729290221595 1106 48-49 0.2366773431396325 1106 50-51 0.03804182375537124 1106 52-53 0.042890549663944455 1106 54-55 0.10523536889108698 1106 56-57 0.6586987544514997 1106 58-59 -8.136103404396046 1106 60-61 -7.479643825420776 1106 62-63 -2.0928104078651515 1106 64-65 -0.27050056630947594 1106 66-67 0.8320786741208082 1106 68-69 -2.245925698708337 1106 70-71 -2.4578427597249792 1106 72-73 -0.5882860539070123 1106 74-75 -0.668337214620756 1106 76-77 2.514481727479666 1106 78-79 4.706514074806101 1106 80-81 3.672040012854545 1106 82-83 1.95016160450135 1106 84-85 1.4991598550264733 1106 86-87 1.3838717749919773 1106 88-89 1.2918560308679439 1106 90-91 2.651776314324131 1106 92-93 2.215886977758455 1106 94-95 -6.109391039238293 1106 96-97 -7.8082997225395285 1106 98-99 -5.796803129670632 1106 100-101 -5.110711508601442 1107 1 0.016216061646979085 1107 2 0.014106614645005067 1107 3 0.034724094175562215 1107 4 -0.04521752633694831 1107 5 0.03855569276350934 1107 6 -0.04195296890591038 1107 7 0.005417392728631398 1107 8 0.056121919301297396 1107 9 0.07230830913074016 1107 10-11 -0.014496582940253688 1107 12-13 0.005036360731985212 1107 14-15 0.018964016868629585 1107 16-17 0.022975153228898648 1107 18-19 0.028443818560361933 1107 20-21 0.059776415196218124 1107 22-23 0.055063366850390594 1107 24-25 0.08916560200927393 1107 26-27 0.08102562443288974 1107 28-29 0.06507818592015013 1107 30-31 0.027963958945683487 1107 32-33 -0.052582456289826496 1107 34-35 -0.04036657826735279 1107 36-37 0.02792027122131202 1107 38-39 -0.05245244080741429 1107 40-41 -0.09942853730562717 1107 42-43 -0.07447836836014687 1107 44-45 -0.08845953409792173 1107 46-47 -0.08792767556018077 1107 48-49 -0.18601419007936926 1107 50-51 -0.060461379082873634 1107 52-53 0.013545552972026087 1107 54-55 0.026032428244818107 1107 56-57 0.48501314745065116 1107 58-59 -8.711538751139742 1107 60-61 -8.335421722958266 1107 62-63 -2.575370229257487 1107 64-65 -0.8318169035687646 1107 66-67 0.36633670292417264 1107 68-69 -2.641276309687367 1107 70-71 -2.5515232418071108 1107 72-73 -1.1995069360159505 1107 74-75 -0.9733829000149257 1107 76-77 2.0956045070354854 1107 78-79 4.0900302999401 1107 80-81 3.1684158789233337 1107 82-83 1.5362730393936452 1107 84-85 1.0322192970049393 1107 86-87 0.9030558298880678 1107 88-89 0.9117770641761425 1107 90-91 2.274217984160444 1107 92-93 1.91864871198187 1107 94-95 -6.993727440129632 1107 96-97 -8.486236392984605 1107 98-99 -6.386010869619234 1107 100-101 -5.495875871728833 1108 1 0.04011855136765874 1108 2 0.006235677187536481 1108 3 -0.10040303980790455 1108 4 -0.05767330511650215 1108 5 0.014618830620761969 1108 6 -0.031043370459130415 1108 7 -0.015194024378047288 1108 8 0.026311076410813428 1108 9 -0.019136684346598543 1108 10-11 -0.03326039556171878 1108 12-13 0.019150927139499174 1108 14-15 -0.14893878393981907 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2312 96-97 0.20386711824469117 2312 98-99 -0.7592843905877587 2312 100-101 -0.23825296411290076 2313 1 -0.3118329096465935 2313 2 -0.31731884325424886 2313 3 -0.2500559366773949 2313 4 -0.172129595406588 2313 5 -0.23702426415946576 2313 6 -0.15389330155423409 2313 7 -0.22257334040426002 2313 8 -0.36980147349930803 2313 9 -0.4517802384927947 2313 10-11 -0.3571196196325843 2313 12-13 -0.3202708878832041 2313 14-15 -0.32382565965109933 2313 16-17 -0.34059474994843697 2313 18-19 -0.43671804967225825 2313 20-21 -0.3699345167424539 2313 22-23 -0.31924113775467333 2313 24-25 -0.2983243790141401 2313 26-27 -0.338932588657471 2313 28-29 -0.3275181563756746 2313 30-31 -0.46204417060746295 2313 32-33 -0.376005313830035 2313 34-35 -0.4912957811594083 2313 36-37 -0.4513908852522306 2313 38-39 -0.5172374536994084 2313 40-41 -0.49541108781683363 2313 42-43 -0.5919564501332957 2313 44-45 -0.5550102200546974 2313 46-47 -0.582785630261597 2313 48-49 -0.8421046984943956 2313 50-51 -0.8227549835904071 2313 52-53 -0.6472917315284832 2313 54-55 -0.7094781415467253 2313 56-57 0.2335460993613907 2313 58-59 3.349303085361541 2313 60-61 3.197016886792081 2313 62-63 0.4621956718190674 2313 64-65 -0.23058029194089613 2313 66-67 -2.341572844474282 2313 68-69 -4.873275891543777 2313 70-71 -3.601472071828539 2313 72-73 -3.155401022166341 2313 74-75 -3.8706774103477386 2313 76-77 0.18280151305419068 2313 78-79 2.8690415275986965 2313 80-81 2.0704941074584795 2313 82-83 0.6385527225560921 2313 84-85 0.304581172374359 2313 86-87 0.2665215402838328 2313 88-89 0.37864227279959906 2313 90-91 2.133396464679713 2313 92-93 3.3099582562694074 2313 94-95 5.641551503732991 2313 96-97 1.1295307083561994 2313 98-99 2.829509783197153 2313 100-101 3.96834666026327 2314 1 -0.3827835253934637 2314 2 -0.311250208221157 2314 3 -0.403444430194579 2314 4 -0.26289330146572354 2314 5 -0.3569074671882646 2314 6 -0.3726054450040337 2314 7 -0.3656046453005146 2314 8 -0.5500865773415597 2314 9 -0.43191807382287806 2314 10-11 -0.45089412841189613 2314 12-13 -0.5046880072002367 2314 14-15 -0.620643505632124 2314 16-17 -0.6520620868738973 2314 18-19 -0.642806269009192 2314 20-21 -0.6141763451803186 2314 22-23 -0.5305540722104212 2314 24-25 -0.6744701289475756 2314 26-27 -0.7922644649064807 2314 28-29 -0.6947890586440906 2314 30-31 -0.7355588533826349 2314 32-33 -0.6769547208936615 2314 34-35 -0.7142529701438534 2314 36-37 -0.5512469792423431 2314 38-39 -0.5666334074277444 2314 40-41 -0.5192906475289618 2314 42-43 -0.6023554428366609 2314 44-45 -0.5647044504551673 2314 46-47 -0.5136537192970252 2314 48-49 -0.5864326342542441 2314 50-51 -0.5801244298953421 2314 52-53 -0.623193836364031 2314 54-55 -0.5925964261979999 2314 56-57 0.2327789717289832 2314 58-59 3.283706913706709 2314 60-61 3.332123250918354 2314 62-63 0.7402382592736672 2314 64-65 -0.2753486019018112 2314 66-67 -1.9787652095947692 2314 68-69 -4.8086187213711735 2314 70-71 -3.6852057625564854 2314 72-73 -3.108135486269809 2314 74-75 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-0.703201801874684 2315 34-35 -0.6267633725708208 2315 36-37 -0.682496791941027 2315 38-39 -0.8521471778205338 2315 40-41 -0.7429530279730869 2315 42-43 -0.6851699419180264 2315 44-45 -0.6494769110527443 2315 46-47 -0.7113868546864808 2315 48-49 -0.7828517212381243 2315 50-51 -0.6137216179550791 2315 52-53 -0.6150625963486291 2315 54-55 -0.5464519517597282 2315 56-57 0.13137778498784058 2315 58-59 3.331511569383494 2315 60-61 3.3318565914748 2315 62-63 0.4593837964307923 2315 64-65 -0.07971162758456529 2315 66-67 -1.9002029690791353 2315 68-69 -4.82533654531024 2315 70-71 -3.63586454451009 2315 72-73 -3.0605221660917543 2315 74-75 -0.5546423868992356 2315 76-77 1.9771557953949888 2315 78-79 3.6584987396580573 2315 80-81 2.578192069686878 2315 82-83 0.7621910332570501 2315 84-85 0.5731855202153682 2315 86-87 0.6379312993002628 2315 88-89 0.6207871672727556 2315 90-91 2.4744440447186804 2315 92-93 3.9901233625628016 2315 94-95 6.566600614589092 2315 96-97 3.857348790532935 2315 98-99 6.241848134971605 2315 100-101 6.403585029542585 2316 1 -0.4911513467644113 2316 2 -0.4838516606932082 2316 3 -0.6165013618369102 2316 4 -0.3986830058626083 2316 5 -0.5527406397607848 2316 6 -0.5549172769847317 2316 7 -0.5287885741151612 2316 8 -0.527117381593456 2316 9 -0.683491592960074 2316 10-11 -0.6948937212028028 2316 12-13 -0.646137964410741 2316 14-15 -0.8270168159584586 2316 16-17 -0.7177024138683663 2316 18-19 -0.7264448542086299 2316 20-21 -0.653078119468411 2316 22-23 -0.9886994509683262 2316 24-25 -0.7732926508641143 2316 26-27 -0.7118095249628453 2316 28-29 -0.7043448753423576 2316 30-31 -0.6353074970649217 2316 32-33 -0.6921202379074174 2316 34-35 -0.5344802100146424 2316 36-37 -0.5620323256652497 2316 38-39 -0.6825112962996442 2316 40-41 -0.851784944974753 2316 42-43 -0.6335626039524769 2316 44-45 -0.6704416825211226 2316 46-47 -0.776288125025097 2316 48-49 -0.7772041072122633 2316 50-51 -0.7487025426857485 2316 52-53 -0.6953596192723168 2316 54-55 -0.49888731852048096 2316 56-57 0.175155806087389 2316 58-59 3.467374772870002 2316 60-61 3.2731479512175774 2316 62-63 0.6441682918271496 2316 64-65 0.05043133598486094 2316 66-67 -1.8067476094229633 2316 68-69 -2.9966249145751505 2316 70-71 -3.140167379845952 2316 72-73 -0.9957942966521536 2316 74-75 5.432692383346037 2316 76-77 5.555180523311247 2316 78-79 4.611456532954588 2316 80-81 3.145512408389223 2316 82-83 1.3183866772251527 2316 84-85 0.7755709383877623 2316 86-87 0.5851195770951776 2316 88-89 0.5598761073619549 2316 90-91 2.0293149245453606 2316 92-93 3.6481067973051324 2316 94-95 6.3020716031497805 2316 96-97 3.497639539890507 2316 98-99 5.883880735276975 2316 100-101 6.019048082510325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 14.0 4 28.0 5 90.0 6 197.0 7 517.0 8 1193.0 9 2193.0 10 3535.0 11 5454.0 12 8066.0 13 10967.0 14 13721.0 15 18547.0 16 24938.0 17 33067.0 18 37584.0 19 38067.0 20 40620.0 21 41839.0 22 42392.0 23 43081.0 24 44761.0 25 47664.0 26 59671.0 27 83953.0 28 119514.0 29 158384.0 30 193104.0 31 215658.0 32 223364.0 33 222244.0 34 227617.0 35 242343.0 36 246035.0 37 188086.0 38 74784.0 39 5075.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.10292561372832 19.831899006936872 14.575560079068254 23.489615300266554 2 15.57705913582686 21.232952059917654 39.38492205841287 23.805066745842616 3 19.342236243737958 25.021317984874713 29.989309738732366 25.64713603265496 4 12.875509956334128 17.42654605517277 37.648738030510934 32.049205957982174 5 13.404336930017788 37.85342283816098 34.8201559610333 13.922084270787932 6 31.487325124982984 37.30220683718552 17.469108325945328 13.741359711886167 7 27.873541865161844 32.605200910839955 21.480446002567717 18.040811221430488 8 25.648173592326867 35.65185594744496 19.93927240930131 18.760698050926862 9 25.713754934022965 16.8030842011941 20.387511633809968 37.095649230972974 10-11 24.539632206064663 26.814432913841753 28.067168928438736 20.57876595165485 12-13 25.659402509592145 24.047903707000888 28.17302280410687 22.119670979300096 14-15 22.531436722358997 25.58274619876157 26.28384971439555 25.601967364483887 16-17 22.09635224350479 28.63530877608802 26.954393065401888 22.313945915005302 18-19 21.90920663731195 27.74368143727862 28.694880128989563 21.652231796419862 20-21 22.806038177290066 26.770987761047984 28.532594900620595 21.89037916104136 22-23 22.34798794866041 26.702398864025135 28.50461857657346 22.444994610740995 24-25 22.450435298263734 26.884845528527062 28.565340355198675 22.099378818010525 26-27 22.33918593330659 26.817871554752866 28.695176824152906 22.147765687787636 28-29 22.09372884164848 27.245914466596638 28.590705030990403 22.069651660764478 30-31 22.35200804638591 26.88133751300014 28.447038689386765 22.319615751227172 32-33 22.26407757418079 26.885127936476174 28.50120151981588 22.349592969527162 34-35 22.694972583295336 26.873428834563352 28.403801680042246 22.02779690209907 36-37 22.53702949896008 26.610812891694895 28.63374022379436 22.218417385550666 38-39 22.270628044527303 27.051574874684352 28.64521768168859 22.032579399099756 40-41 22.455084639045968 26.909229621567327 28.573309041690266 22.062376697696443 42-43 22.13262463707547 26.959810883409403 28.267591138473776 22.639973341041355 44-45 22.447796742108707 26.911038585873243 28.192815582672942 22.44834908934511 46-47 22.655968374964054 27.11419337710889 27.89044057731303 22.339397670614026 48-49 22.55062586014811 27.110045940837274 27.932143196389532 22.407185002625084 50-51 22.238832070567618 27.295049074830835 28.090922812985504 22.375196041616046 52-53 22.562031340387886 27.389019434471262 27.590982152076286 22.457967073064562 54-55 22.45283185537789 27.109964964119413 27.732837332149646 22.70436584835305 56-57 22.404597189154565 27.35027522209655 27.774377701718333 22.470749887030557 58-59 21.622123835111303 26.812883795879966 29.263767742193657 22.30122462681507 60-61 21.800191427262625 27.117631794495857 28.87558076912713 22.20659600911439 62-63 22.40922888003611 27.141665743906195 28.074236294615773 22.37486908144192 64-65 22.286962118239856 27.332608987153247 27.829921015364857 22.550507879242037 66-67 22.15482388185056 26.914217943989822 28.418294483509072 22.512663690650545 68-69 21.47287873136437 26.374449455389403 30.012338981007385 22.140332832238837 70-71 22.4105022359847 26.341327188926204 29.015225184034687 22.232945391054404 72-73 21.706589938664923 26.5025707838854 29.285640302279457 22.505198975170224 74-75 21.713116343962565 26.980630301038328 28.738971241054813 22.567282113944295 76-77 20.75563684070088 27.18611951139969 29.047462865783153 23.01078078211628 78-79 22.007602810054237 27.131536456844866 28.09935852793083 22.761502205170068 80-81 22.08703831690886 27.31217840153742 27.66171834690938 22.93906493464435 82-83 22.40758057775842 27.258873706576885 27.521053073020283 22.81249264264441 84-85 22.262690145951233 27.279171714938478 27.421538038203103 23.03660010090719 86-87 22.158878650790513 27.30966502442214 27.58797902758401 22.94347729720334 88-89 22.25404277344246 27.224192153456723 27.331241637908615 23.190523435192205 90-91 22.059246625460275 27.132862461174085 27.73589944081086 23.071991472554775 92-93 21.919700244673813 27.281893861806683 27.68849959466411 23.1099062988554 94-95 20.941269582469676 27.148997427320026 28.526608686351057 23.383124303859244 96-97 20.606944686007957 27.156333047197457 28.65910744308608 23.577614823708508 98-99 21.234795483318834 27.12613322902496 28.02677412947333 23.61229715818288 100-101 20.473399226281064 27.108946455860046 28.542359474634743 23.87529484322415 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 385.0 1 275.5 2 185.0 3 221.0 4 250.0 5 302.0 6 381.5 7 582.0 8 919.0 9 1200.5 10 1323.5 11 1425.0 12 1575.0 13 1756.5 14 1958.0 15 2185.5 16 2472.0 17 2740.0 18 3076.5 19 3435.0 20 3878.0 21 4599.0 22 5558.0 23 6760.5 24 8429.5 25 10477.0 26 13290.0 27 17133.0 28 21374.0 29 25975.0 30 31140.0 31 37255.5 32 43874.0 33 50715.0 34 57776.0 35 64632.0 36 71344.0 37 77411.5 38 81573.5 39 84618.0 40 87752.5 41 90781.5 42 93440.5 43 96045.0 44 98693.5 45 101229.5 46 104617.5 47 108314.0 48 111490.0 49 110130.5 50 105171.5 51 101069.0 52 97361.5 53 93995.0 54 89119.0 55 82057.0 56 74403.5 57 66429.5 58 58280.0 59 49589.5 60 40347.0 61 31611.0 62 23879.5 63 17496.5 64 12563.0 65 9008.0 66 6430.0 67 4550.5 68 3101.5 69 2008.0 70 1277.5 71 766.5 72 466.5 73 275.0 74 152.0 75 90.5 76 48.0 77 24.0 78 9.0 79 7.0 80 5.0 81 4.0 82 5.0 83 2.5 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026412887134569615 2 0.013721458079658029 3 2.9429400707041355E-4 4 0.0 5 0.06599543108554023 6 0.0 7 0.0 8 3.6786750883801694E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 4.598343860475211E-4 16-17 1.8393375441900847E-5 18-19 3.862608842799177E-4 20-21 0.0 22-23 0.0 24-25 1.4714700353520677E-4 26-27 2.023271298609093E-4 28-29 0.0010484224001883482 30-31 0.004819064365778022 32-33 0.005370865629035047 34-35 0.001673797165212977 36-37 0.09301529960969257 38-39 0.08565794943293224 40-41 0.09137828919536341 42-43 0.09509375103462737 44-45 0.09884599962477512 46-47 0.0936958545010429 48-49 0.08343235100446222 50-51 0.07762004436482156 52-53 0.06546202319772511 54-55 0.05300970802355824 56-57 0.07090646232852776 58-59 0.7175991494903196 60-61 0.3401670854225142 62-63 0.00283257981805273 64-65 0.0020784514249347955 66-67 1.0990593627799012 68-69 2.3609736717223924 70-71 2.32359833282445 72-73 1.797161534301806 74-75 1.2829195436971421 76-77 0.7375743552202239 78-79 0.5624878143887697 80-81 0.039876837958041034 82-83 7.357350176760339E-5 84-85 0.0011219959019559515 86-87 0.0013795031581425633 88-89 3.6786750883801694E-5 90-91 0.3310991513296571 92-93 0.05472029193965501 94-95 0.9508271500936224 96-97 1.6485982408575728 98-99 0.6402550057571266 100-101 0.568373694530178 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2718370.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.558186002655006 #Duplication Level Percentage of deduplicated Percentage of total 1 76.79572028565146 42.6663091183809 2 13.01298984261289 14.459562202530948 3 4.301147695232157 7.168918910297972 4 1.9516102784013662 4.337117074084658 5 1.0633910104822715 2.9540037776962653 6 0.6666314002645559 2.2222098798665137 7 0.42022288137450753 1.6342774704183567 8 0.290888503537698 1.2928990068465116 9 0.2139670415274699 1.0698858622458884 >10 1.1363500934800213 11.671142361601248 >50 0.09687892427432213 3.714557886671828 >100 0.047285245515714794 4.53114431439502 >500 0.001856143956334492 0.6357225755854807 >1k 8.617811225507321E-4 0.903660802621957 >5k 1.9887256674247665E-4 0.738588756756632 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7776 0.28605377487244193 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6141 0.22590743717742617 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6137 0.22576029017389096 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4145 0.152481082413358 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3217 0.11834297759319003 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.678675088380169E-5 0.0 0.0 0.0 0.0 3 3.678675088380169E-5 0.0 0.0 0.0 0.0 4 3.678675088380169E-5 0.0 0.0 0.0 0.0 5 3.678675088380169E-5 0.0 0.0 0.0 0.0 6 3.678675088380169E-5 0.0 0.0 0.0 0.0 7 3.678675088380169E-5 0.0 0.0 0.0 0.0 8 3.678675088380169E-5 0.0 0.0 0.0 0.0 9 3.678675088380169E-5 0.0 0.0 0.0 0.0 10-11 3.678675088380169E-5 0.0 0.0 1.8393375441900843E-5 3.678675088380169E-5 12-13 3.678675088380169E-5 0.0 0.0 3.678675088380169E-5 3.678675088380169E-5 14-15 3.678675088380169E-5 0.0 0.0 5.518012632570253E-5 5.518012632570253E-5 16-17 3.678675088380169E-5 0.0 0.0 1.655403789771076E-4 1.1036025265140507E-4 18-19 3.678675088380169E-5 0.0 0.0 2.5750725618661183E-4 1.4714700353520675E-4 20-21 3.678675088380169E-5 0.0 0.0 2.7590063162851266E-4 1.8393375441900844E-4 22-23 3.678675088380169E-5 0.0 0.0 4.0465425972181855E-4 1.8393375441900844E-4 24-25 3.678675088380169E-5 0.0 0.0 6.989482667922321E-4 1.8393375441900844E-4 26-27 3.678675088380169E-5 0.0 0.0 0.0011403892773978524 1.8393375441900844E-4 28-29 3.678675088380169E-5 0.0 0.0 0.0024095321828890106 1.8393375441900844E-4 30-31 3.678675088380169E-5 0.0 0.0 0.006548041657316701 1.8393375441900844E-4 32-33 3.678675088380169E-5 0.0 0.0 0.014825060606172082 2.2072050530281013E-4 34-35 3.678675088380169E-5 0.0 0.0 0.028197044552434 2.5750725618661183E-4 36-37 3.678675088380169E-5 0.0 0.0 0.04852172441573443 2.5750725618661183E-4 38-39 7.357350176760337E-5 0.0 0.0 0.0785213197614747 2.942940070704135E-4 40-41 7.357350176760337E-5 0.0 0.0 0.11315604571857399 2.942940070704135E-4 42-43 9.196687720950422E-5 0.0 0.0 0.14874722719865213 2.942940070704135E-4 44-45 1.1036025265140507E-4 0.0 0.0 0.1943811916700081 3.310807579542152E-4 46-47 1.8393375441900844E-4 0.0 0.0 0.24926702398864026 3.678675088380169E-4 48-49 2.2072050530281013E-4 0.0 0.0 0.3047046575705294 3.678675088380169E-4 50-51 2.2072050530281013E-4 0.0 0.0 0.3616321545632125 3.678675088380169E-4 52-53 2.5750725618661183E-4 0.0 0.0 0.41664674050993794 4.2304763516371943E-4 54-55 2.942940070704135E-4 0.0 0.0 0.48065568704775286 4.4144101060562027E-4 56-57 2.942940070704135E-4 0.0 0.0 0.544995714343522 4.4144101060562027E-4 58-59 2.942940070704135E-4 3.678675088380169E-5 0.0 0.591733281341392 4.4144101060562027E-4 60-61 2.942940070704135E-4 3.678675088380169E-5 0.0 0.6332655230892041 4.4144101060562027E-4 62-63 3.310807579542152E-4 3.678675088380169E-5 0.0 0.6678450689199777 4.78227761489422E-4 64-65 3.678675088380169E-4 9.196687720950422E-5 0.0 0.7071701056147618 4.78227761489422E-4 66-67 4.046542597218186E-4 1.1036025265140507E-4 1.8393375441900843E-5 0.7422094858315829 4.966211369313229E-4 68-69 4.2304763516371943E-4 1.1036025265140507E-4 3.678675088380169E-5 0.785764998878004 5.150145123732237E-4 70-71 4.4144101060562027E-4 1.1036025265140507E-4 3.678675088380169E-5 0.8535629807568506 5.518012632570253E-4 72-73 4.4144101060562027E-4 1.1036025265140507E-4 3.678675088380169E-5 0.9422926238885803 5.701946386989262E-4 74-75 4.4144101060562027E-4 1.1036025265140507E-4 3.678675088380169E-5 1.0662087942406662 5.88588014140827E-4 76-77 4.4144101060562027E-4 1.1036025265140507E-4 3.678675088380169E-5 1.2279049577504166 5.88588014140827E-4 78-79 4.4144101060562027E-4 1.1036025265140507E-4 3.678675088380169E-5 1.4386010734373909 5.88588014140827E-4 80-81 4.78227761489422E-4 1.1036025265140507E-4 3.678675088380169E-5 1.6895786813421279 5.88588014140827E-4 82-83 4.78227761489422E-4 1.1036025265140507E-4 3.678675088380169E-5 1.9383858709447206 5.88588014140827E-4 84-85 4.966211369313229E-4 1.4714700353520675E-4 3.678675088380169E-5 2.161258401174233 5.88588014140827E-4 86-87 5.150145123732237E-4 1.8393375441900844E-4 3.678675088380169E-5 2.3665468644812884 6.253747650246287E-4 88-89 5.334078878151244E-4 1.8393375441900844E-4 3.678675088380169E-5 2.5719089012901115 6.621615159084304E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8630 0.0 23.197577 1 TACATGG 8450 0.0 23.123594 2 ACATGGG 8355 0.0 22.027128 3 GTATCAA 15725 0.0 20.057343 1 GAGTACT 7210 0.0 19.361567 12-13 GGTATCA 12455 0.0 19.25784 1 AGAGTAC 12130 0.0 18.46787 10-11 AGTACTT 7565 0.0 18.234798 12-13 GTACTTT 7670 0.0 18.169634 14-15 AACGCAG 17600 0.0 17.792274 6 TCAACGC 17640 0.0 17.735634 4 CAACGCA 17715 0.0 17.660545 5 ATCAACG 17720 0.0 17.629255 3 TATCAAC 18310 0.0 17.115572 2 CATGGGA 4820 0.0 17.03468 4 CATGGGG 4275 0.0 16.115675 4 CGCAGAG 19030 0.0 16.009205 8 ACGCAGA 19265 0.0 15.985278 7 AGTACAT 6440 0.0 15.903238 2 TTACGCG 110 4.9275014E-4 15.40826 62-63 >>END_MODULE