FastQCFastQC Report
Fri 27 May 2016
SRR939577_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939577_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015362
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83340.41352372427385253No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA60250.2989537363510873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56380.27975123079625397No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA47680.236582807455931No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39810.19753275094002964No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30100.14935282098203698No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT22640.11233713843964509No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA21010.10424926142300986No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG20240.10042860786300427No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG700.00843339827.1161772
GTACATG100950.026.6058181
TACCGAT909.004034E-426.3616437
TACATGG101950.026.2052122
ACATGGG102050.024.2730313
GAGTACT69300.021.91260512-13
CATGGGA71050.021.7068394
AGTACTT73200.021.23134412-13
TCCCGTA1200.004791902419.7722112
GTACTTT77050.019.73983414-15
GTATTAC3951.8189894E-1219.2215961
CATGGGG33050.019.0966644
GTATCAA209200.018.350731
GTATAGG6300.018.0774521
ATGGGAT28400.018.0465225
TACCTGG13800.017.5370922
CATTCCG3251.0148142E-817.5220939
ACTTTTT93100.017.1784716-17
AGTACAT76200.017.0632632
TACTTTT90600.016.65662414-15
Produced by FastQC (version 0.11.5)