FastQCFastQC Report
Fri 27 May 2016
SRR939577_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939577_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015362
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86880.4310888068743977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65070.3228700352591743No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA62370.30947293836045336No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA52650.26124338952505805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47590.236136237559307No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33660.16701714133738754No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT33440.1659255260345288No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA25150.12479147666771528No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG23730.11774559607653613No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT21670.1075241073315861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG100350.026.0829221
TACATGG101500.024.9488372
ACATGGG99250.024.3750273
GAGTACT68700.021.00500312-13
CATGGGA69050.020.5852324
CATGGGG32250.020.577944
AGTACTT71800.019.6725712-13
GTATCAA183200.019.4265271
GTACTTT75450.019.1255914-15
GTATAGG7650.019.0765171
GGTATCA142250.017.8375551
ATCAACG204050.017.2765793
TCAACGC204150.017.2450624
AACGCAG205250.017.2450186
AGTACAT76050.017.205992
CAACGCA206400.017.1026765
TATCAAC212150.016.6621482
CATTCCG5400.016.5386839
ACTTTTT88300.016.501816-17
ATGGGAT27500.016.430515
Produced by FastQC (version 0.11.5)