##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939577_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2015362 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.568896803651157 31.0 31.0 34.0 30.0 34.0 2 31.77106445392937 31.0 31.0 34.0 30.0 34.0 3 31.81817112756914 31.0 31.0 34.0 30.0 34.0 4 35.591250107921056 37.0 35.0 37.0 33.0 37.0 5 35.33151364370272 37.0 35.0 37.0 33.0 37.0 6 35.35964109673597 37.0 35.0 37.0 33.0 37.0 7 35.29134517769016 37.0 35.0 37.0 32.0 37.0 8 35.25933653606647 37.0 35.0 37.0 32.0 37.0 9 36.86562314859564 39.0 37.0 39.0 33.0 39.0 10-11 36.74873943241958 39.0 36.0 39.0 32.0 39.0 12-13 36.694245996500875 39.0 35.0 39.0 32.0 39.0 14-15 37.734209536549756 39.5 37.0 41.0 32.0 41.0 16-17 37.704530749314515 39.0 37.0 41.0 32.0 41.0 18-19 37.71496336638282 39.5 37.0 41.0 32.0 41.0 20-21 37.664258827942575 39.0 37.0 41.0 32.0 41.0 22-23 37.52461542889069 39.0 36.0 41.0 32.0 41.0 24-25 37.39920049102841 39.0 36.0 41.0 32.0 41.0 26-27 37.12758303471039 39.0 36.0 40.5 31.0 41.0 28-29 36.92634127268451 39.0 36.0 40.0 30.5 41.0 30-31 36.697831456581994 38.5 35.5 40.0 30.0 41.0 32-33 36.53043200179422 38.0 35.0 40.0 30.0 41.0 34-35 36.51574134076161 38.0 35.0 40.0 30.0 41.0 36-37 36.391753441813435 38.0 35.0 40.0 30.0 41.0 38-39 36.41219840405843 39.0 35.0 40.0 30.0 41.0 40-41 36.31518655209337 39.0 35.0 40.0 30.0 41.0 42-43 36.13649954697965 38.5 35.0 40.0 30.0 41.0 44-45 35.92303566307194 38.0 35.0 40.0 29.0 41.0 46-47 35.59443936126611 38.0 35.0 40.0 27.5 41.0 48-49 35.288660796422676 38.0 34.0 40.0 27.0 41.0 50-51 35.04688661391849 38.0 34.0 40.0 26.0 41.0 52-53 34.7997347374814 38.0 34.0 40.0 26.0 41.0 54-55 34.512683081252895 38.0 33.5 40.0 25.0 41.0 56-57 32.43561429658791 37.0 30.5 39.5 16.0 41.0 58-59 27.31012393803198 32.5 17.5 38.5 6.5 40.0 60-61 28.091559481621665 33.0 19.0 38.0 8.0 40.0 62-63 31.275478053074337 34.5 29.5 38.5 10.0 40.0 64-65 31.51173486450573 35.5 30.5 39.0 5.0 40.0 66-67 24.759856541901655 27.0 18.0 35.5 2.0 39.0 68-69 17.249391176374267 13.0 7.0 28.0 2.0 35.0 70-71 16.118456634589716 12.5 7.0 26.5 2.0 33.5 72-73 17.220307319479083 13.0 7.0 28.5 2.0 34.0 74-75 18.234356904615645 17.0 8.5 29.0 2.0 34.5 76-77 19.389380419001647 21.0 8.0 30.0 2.0 34.5 78-79 22.46074898703062 27.5 10.5 33.5 2.0 35.5 80-81 26.17483360309463 31.5 22.5 34.5 2.0 36.0 82-83 27.538259379704492 33.5 26.5 35.0 2.0 36.0 84-85 27.60821256925555 34.0 27.0 35.0 2.0 36.0 86-87 27.36909671810821 34.0 26.5 35.0 2.0 36.0 88-89 26.93615018046386 33.0 26.0 35.0 2.0 35.5 90-91 25.528915400806405 32.5 16.0 35.0 2.0 35.0 92-93 23.00338103030622 31.5 2.0 34.5 2.0 35.0 94-95 15.664033806333553 4.5 2.0 32.0 2.0 35.0 96-97 13.092547889659524 2.0 2.0 30.0 2.0 34.0 98-99 12.748417157810856 2.0 2.0 29.5 2.0 34.0 100-101 12.472940097114066 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3664648924865972 1101 2 0.3191520597182915 1101 3 0.42198811168188044 1101 4 0.21115135514780548 1101 5 0.40328379208372667 1101 6 0.37492547938307297 1101 7 0.4328604335395454 1101 8 0.3652118710942318 1101 9 0.3856673126904724 1101 10-11 0.4321039770960411 1101 12-13 0.4389626132305793 1101 14-15 0.5364096133973248 1101 16-17 0.46020848401693826 1101 18-19 0.45442048922939904 1101 20-21 0.5358569185736144 1101 22-23 0.649536863772255 1101 24-25 0.4673612017610367 1101 26-27 0.49829611955292563 1101 28-29 0.5409943984262213 1101 30-31 0.5908495268101106 1101 32-33 0.4895245634394314 1101 34-35 0.4838683269780759 1101 36-37 0.3138128936087625 1101 38-39 0.5735625784634237 1101 40-41 0.610440840684717 1101 42-43 0.8318028069681347 1101 44-45 0.5343926687210043 1101 46-47 0.47591743086722715 1101 48-49 0.45722569047287465 1101 50-51 0.4894454706659914 1101 52-53 0.19479759679634867 1101 54-55 0.2035812187073347 1101 56-57 -1.7873905421334797 1101 58-59 -9.84217535675743 1101 60-61 -10.206475089057346 1101 62-63 -4.284721813281411 1101 64-65 -5.247393337973616 1101 66-67 -10.097160236743187 1101 68-69 -5.04521118275208 1101 70-71 -4.609863951904956 1101 72-73 -4.937071997713657 1101 74-75 -6.841619861720751 1101 76-77 -7.886843264337495 1101 78-79 -11.178244055438903 1101 80-81 -8.112222333829646 1101 82-83 -4.532121937846341 1101 84-85 -3.7033373599766293 1101 86-87 -3.622052440618134 1101 88-89 -3.6854851946921556 1101 90-91 -9.717646318853092 1101 92-93 -16.809567792280063 1101 94-95 -11.202845447180227 1101 96-97 -9.49056451780588 1101 98-99 -9.608320616503732 1101 100-101 -9.401212623597079 1102 1 0.4138711743681256 1102 2 0.3549803462739618 1102 3 0.3394461275566165 1102 4 0.2693165710920056 1102 5 0.25713374179684223 1102 6 0.283614619585812 1102 7 0.35942363221978013 1102 8 0.3955926766974187 1102 9 0.5001211391970912 1102 10-11 0.49889495005688644 1102 12-13 0.4284935342403742 1102 14-15 0.506164112084079 1102 16-17 0.5664949962775339 1102 18-19 0.4641833811234406 1102 20-21 0.4809945744087045 1102 22-23 0.5233033155438704 1102 24-25 0.4435841137698304 1102 26-27 0.5714031257103613 1102 28-29 0.5577492661246737 1102 30-31 0.5342426979230126 1102 32-33 0.6001612171545077 1102 34-35 0.4251330964904696 1102 36-37 0.5817256621371385 1102 38-39 0.40445985573591514 1102 40-41 0.5107599272722183 1102 42-43 0.3659190239470149 1102 44-45 0.5339563666523688 1102 46-47 0.485889863522587 1102 48-49 0.4230583906978964 1102 50-51 0.5608464850863868 1102 52-53 0.5155694824461321 1102 54-55 0.4728916009600326 1102 56-57 -2.2923038967392557 1102 58-59 -10.214444923421926 1102 60-61 -10.309402581876089 1102 62-63 -3.879096627871121 1102 64-65 -4.980668931829211 1102 66-67 -6.6372428664218575 1102 68-69 -3.9461370403425065 1102 70-71 -4.066987107750897 1102 72-73 -4.369704498238232 1102 74-75 -5.1062008163757255 1102 76-77 -7.120885534265936 1102 78-79 -9.745842223404345 1102 80-81 -5.221071379102316 1102 82-83 -2.714882868913506 1102 84-85 -2.3017504649568394 1102 86-87 -2.2325627620924458 1102 88-89 -2.297275624988739 1102 90-91 -8.553508612369338 1102 92-93 -13.229246100904081 1102 94-95 -10.649760292303846 1102 96-97 -9.37816167279803 1102 98-99 -9.494037403641865 1102 100-101 -9.415431729720503 1103 1 0.23347066613475675 1103 2 0.26972935555394884 1103 3 0.26757606496844133 1103 4 0.2379797115550346 1103 5 0.2251745411404258 1103 6 0.19635212921855327 1103 7 0.11285298367094754 1103 8 0.23277859225530761 1103 9 0.39485851908631986 1103 10-11 0.44650471104605316 1103 12-13 0.2477782910651527 1103 14-15 0.45687274689483104 1103 16-17 0.3749225263294278 1103 18-19 0.41641650217009385 1103 20-21 0.3236038161862069 1103 22-23 0.4768715353227222 1103 24-25 0.5631939660071836 1103 26-27 0.5094178076379592 1103 28-29 0.42106103336196554 1103 30-31 0.4992484258850851 1103 32-33 0.4628424451934592 1103 34-35 0.45293385133155084 1103 36-37 0.30049721922321027 1103 38-39 0.25472503388492385 1103 40-41 0.40918264065574306 1103 42-43 0.24091234073052448 1103 44-45 0.3092162516939183 1103 46-47 0.5018189684373198 1103 48-49 0.4637772980579413 1103 50-51 0.33236388770686887 1103 52-53 0.3506111845289013 1103 54-55 0.16615845935877616 1103 56-57 -2.9802284592054065 1103 58-59 -10.169865552592402 1103 60-61 -10.611800365495757 1103 62-63 -4.343178586798508 1103 64-65 -5.682096205483219 1103 66-67 -6.520232755695513 1103 68-69 -4.13512295699347 1103 70-71 -4.031826854318574 1103 72-73 -4.285365632904629 1103 74-75 -5.175842902508782 1103 76-77 -6.761842471064449 1103 78-79 -9.26590541752321 1103 80-81 -5.148791531382226 1103 82-83 -2.998865034646343 1103 84-85 -2.332043911032372 1103 86-87 -2.1096815199385404 1103 88-89 -2.2427301229181893 1103 90-91 -5.1212487441992 1103 92-93 -10.31024490255881 1103 94-95 -8.776953842768249 1103 96-97 -8.214922001129903 1103 98-99 -8.561303116210567 1103 100-101 -8.532237601350943 1104 1 0.2570320002370714 1104 2 0.26229635713480093 1104 3 0.22723070777513854 1104 4 0.2056000003904046 1104 5 0.2798685431864385 1104 6 0.24193871189724803 1104 7 0.29238784374748406 1104 8 0.25096486416290986 1104 9 0.2181310808064154 1104 10-11 0.38984184729137894 1104 12-13 0.4048416302438085 1104 14-15 0.4646610311667061 1104 16-17 0.3769633674447874 1104 18-19 0.3786013335898204 1104 20-21 0.3924196154860269 1104 22-23 0.3872432279814504 1104 24-25 0.4445603195934211 1104 26-27 0.5783370773349077 1104 28-29 0.5886409012617548 1104 30-31 0.496104725297819 1104 32-33 0.5955642609819662 1104 34-35 0.6916219501664713 1104 36-37 0.6416925407417224 1104 38-39 0.48534266668018233 1104 40-41 0.39905142669605453 1104 42-43 0.5724927880645438 1104 44-45 0.4930883208949197 1104 46-47 0.5372973930600438 1104 48-49 0.4062269507123162 1104 50-51 0.37597351099365284 1104 52-53 0.32639495029351195 1104 54-55 0.33119936615533874 1104 56-57 -2.798077372631276 1104 58-59 -10.156942212460013 1104 60-61 -10.216903570468812 1104 62-63 -3.7611931428340277 1104 64-65 -4.64932780552245 1104 66-67 -5.73362841569719 1104 68-69 -3.6670038733632957 1104 70-71 -3.7980618094978986 1104 72-73 -4.137254732433254 1104 74-75 -4.898907196314324 1104 76-77 -6.462681323568749 1104 78-79 -8.927780917442682 1104 80-81 -4.458992970250566 1104 82-83 -2.0995857059622836 1104 84-85 -1.5593266479413401 1104 86-87 -1.3996516844744669 1104 88-89 -1.3533459382334314 1104 90-91 -4.00226824701944 1104 92-93 -6.450985561656243 1104 94-95 -7.311248093683062 1104 96-97 -7.574049079872673 1104 98-99 -7.949788795457733 1104 100-101 -8.109549107529027 1105 1 0.2994612307163216 1105 2 0.3073378700077214 1105 3 0.307846382933473 1105 4 0.18628695288620634 1105 5 0.12560571921309815 1105 6 0.2606908735494926 1105 7 0.19864350521764607 1105 8 0.2306206284773964 1105 9 0.42207484948354335 1105 10-11 0.35273096756982625 1105 12-13 0.3513726473229184 1105 14-15 0.43245195524673363 1105 16-17 0.4382124684719457 1105 18-19 0.46274084502439194 1105 20-21 0.3856722867849598 1105 22-23 0.43614460539998845 1105 24-25 0.526117743141846 1105 26-27 0.43151651322830276 1105 28-29 0.43853554073018586 1105 30-31 0.6250252076047573 1105 32-33 0.5900809841642101 1105 34-35 0.47068988231850284 1105 36-37 0.4258949116510493 1105 38-39 0.43520125573659385 1105 40-41 0.43049008518153187 1105 42-43 0.28893273667898 1105 44-45 0.2904177539518642 1105 46-47 0.4057385512847489 1105 48-49 0.3313855176260958 1105 50-51 0.25734844076854557 1105 52-53 0.2992544342053307 1105 54-55 0.07861636328411947 1105 56-57 1.3318848201853584 1105 58-59 -9.017464861085617 1105 60-61 -10.327952564259725 1105 62-63 -3.975377424488393 1105 64-65 -4.845514330720224 1105 66-67 -6.407000016756378 1105 68-69 -3.980521451141602 1105 70-71 -4.082170165690609 1105 72-73 -2.2986471201234124 1105 74-75 -4.594925135511735 1105 76-77 -6.3238261620464336 1105 78-79 -8.917373648435241 1105 80-81 -4.345354339946628 1105 82-83 -1.9403986610529813 1105 84-85 -1.4348170799423983 1105 86-87 -1.3505595326674644 1105 88-89 -1.50193608347816 1105 90-91 -4.234323788443561 1105 92-93 -6.55973657042729 1105 94-95 -7.7416252779811865 1105 96-97 -8.13878610401181 1105 98-99 -8.474863961481777 1105 100-101 -8.514386999623007 1106 1 0.1392596198173095 1106 2 0.18303923593458293 1106 3 0.22215678040375408 1106 4 0.11266157803147081 1106 5 0.06178349088958157 1106 6 0.16499670294000168 1106 7 0.11568386290066002 1106 8 0.057750051881583886 1106 9 0.04804497471520364 1106 10-11 0.12392354408515871 1106 12-13 0.23547584805571375 1106 14-15 0.31030777434131096 1106 16-17 0.3643656719266488 1106 18-19 0.2548173568102712 1106 20-21 0.2539576237222647 1106 22-23 0.22825200497916143 1106 24-25 0.26160024360930123 1106 26-27 0.28427818964257767 1106 28-29 0.3084271612624008 1106 30-31 0.3318976824388997 1106 32-33 0.3262163886759524 1106 34-35 0.5003149447449786 1106 36-37 0.4786461737901888 1106 38-39 0.37422733166139466 1106 40-41 0.290764004286288 1106 42-43 0.28317944138930784 1106 44-45 0.36210607363224057 1106 46-47 0.3789322070976908 1106 48-49 0.5393106626567103 1106 50-51 0.4896885520592775 1106 52-53 0.5805550114875402 1106 54-55 0.46210997388963904 1106 56-57 1.7879972896230143 1106 58-59 -8.73515859618906 1106 60-61 -9.82711220326308 1106 62-63 -3.707920140768035 1106 64-65 -5.449624300731845 1106 66-67 -6.94541508242548 1106 68-69 -4.319254625578344 1106 70-71 -4.010464922813144 1106 72-73 -4.3995970676970035 1106 74-75 -5.068614652833752 1106 76-77 -6.8034528812894095 1106 78-79 -9.226488979431673 1106 80-81 -4.690433594947255 1106 82-83 -2.2548716632281476 1106 84-85 -1.6158223888558325 1106 86-87 -1.4288239937168932 1106 88-89 -1.4463639729069584 1106 90-91 -3.9443200724408456 1106 92-93 -6.389080920801373 1106 94-95 -7.2918281196581685 1106 96-97 -7.304958763225958 1106 98-99 -6.92358513583331 1106 100-101 -7.419813665374479 1107 1 -0.015064257711028262 1107 2 -0.0704856214411329 1107 3 -0.046820353403759896 1107 4 0.022933305081927813 1107 5 -0.12097263084117316 1107 6 -0.10112030454314436 1107 7 -0.060668057896556604 1107 8 0.003842470567221312 1107 9 -0.12592771365999056 1107 10-11 -0.1383085723110753 1107 12-13 -0.09615143485901001 1107 14-15 -0.08513230030490604 1107 16-17 -0.13969229002785966 1107 18-19 -0.02249403646230519 1107 20-21 -0.04851561107064839 1107 22-23 0.10489588880519563 1107 24-25 0.10833863917869024 1107 26-27 0.07163892258323301 1107 28-29 0.1144981863050063 1107 30-31 -0.02244263392770307 1107 32-33 -0.0031224227265767013 1107 34-35 -0.1086600821128414 1107 36-37 -0.1392388191712044 1107 38-39 -0.06172521797926578 1107 40-41 -0.24186894192398256 1107 42-43 -0.4024630856537428 1107 44-45 -0.25677786816964243 1107 46-47 -0.3357348253910146 1107 48-49 -0.3987398068920811 1107 50-51 -0.4628836630626054 1107 52-53 -0.440823697670055 1107 54-55 -0.4263607279329875 1107 56-57 0.6062692898823272 1107 58-59 -9.285402053068367 1107 60-61 -10.897544753088965 1107 62-63 -4.749214155178645 1107 64-65 -5.995031266168606 1107 66-67 -7.433995299325531 1107 68-69 -4.116217029260643 1107 70-71 -4.453998092221161 1107 72-73 -2.8715894548941563 1107 74-75 -5.017826630495454 1107 76-77 -6.94507243100416 1107 78-79 -7.973538439496872 1107 80-81 -3.2324483713790535 1107 82-83 -2.037970916765971 1107 84-85 -1.900880179475969 1107 86-87 -1.8000806850186635 1107 88-89 -1.5568303033878053 1107 90-91 -1.1054378208293762 1107 92-93 -3.6276495610547634 1107 94-95 -5.956822308181543 1107 96-97 -6.378338727292046 1107 98-99 -6.069364753526131 1107 100-101 -6.868238315296684 1108 1 -0.05914012669498803 1108 2 -0.13635571948034908 1108 3 -0.1464192838850451 1108 4 -0.04307605320683905 1108 5 0.03430254741194716 1108 6 -0.0829189899263909 1108 7 -0.1116512352584138 1108 8 -0.009679699664509656 1108 9 -0.047134264462130204 1108 10-11 -0.06590020555528042 1108 12-13 0.024998019240811686 1108 14-15 -0.05397281946711985 1108 16-17 -0.08390463405638116 1108 18-19 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13.3502756205006 2316 98-99 12.934794222016093 2316 100-101 11.8093170607993 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 32.0 5 84.0 6 185.0 7 481.0 8 1026.0 9 1701.0 10 2976.0 11 4785.0 12 6957.0 13 10011.0 14 12564.0 15 17362.0 16 23390.0 17 32001.0 18 34189.0 19 33833.0 20 34868.0 21 34719.0 22 34959.0 23 29700.0 24 30118.0 25 34968.0 26 49306.0 27 73880.0 28 105182.0 29 142915.0 30 177983.0 31 194336.0 32 194164.0 33 177886.0 34 157414.0 35 141977.0 36 115079.0 37 74183.0 38 28218.0 39 1921.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.90337725967895 19.95674201245925 14.2984126164829 22.841468111378894 2 15.213204692483165 21.776498184071936 39.46952768998766 23.54076943345724 3 19.0023593858154 26.480297515822276 30.72610036445192 23.791242733910405 4 12.401097172617128 17.464257041662986 38.2851815207392 31.849464264980682 5 12.64979867832807 39.108827991339915 34.592891951158066 13.648481379173951 6 30.220823851992844 37.734411981569565 17.996866071703245 14.047898094734347 7 27.18866387279308 33.97240793465392 21.60584550070905 17.233082691843947 8 25.686897090353895 35.00536636413572 20.398634269010895 18.909102276499492 9 26.548778829808246 16.687820848066004 20.768080374642373 35.99531994748338 10-11 24.160126071643706 27.00122856340449 28.228824399785253 20.609820965166556 12-13 25.889567234075066 24.678296008359787 28.236341659711755 21.195795097853388 14-15 22.74524504843212 25.605745711717564 27.130967305125942 24.518041934724376 16-17 21.389780099059127 29.103630017833026 27.71164684061722 21.79494304249063 18-19 21.293733800975062 28.065748038626342 29.848704487360944 20.79181367303765 20-21 22.375012528766543 27.05102110687807 29.291586325434338 21.282380038921048 22-23 21.93168353382727 27.111016614434373 29.053799924182126 21.903499927556222 24-25 21.999273579634824 27.12515170971766 29.43037032552961 21.445204385117908 26-27 22.0184562834277 27.06651972826651 29.529209961312063 21.385814026993728 28-29 21.461951915652918 27.260069508537722 29.81694464127283 21.461033934536534 30-31 21.641223504891165 27.316216836528977 29.66720220335093 21.375357455228926 32-33 21.529251228104997 27.349029921103558 29.346251178484593 21.775467672306853 34-35 22.008803332771784 27.01458546653669 29.21782529743688 21.758785903254644 36-37 22.17474703796163 26.79354735995283 29.368930531715165 21.662775070370376 38-39 22.008784925792586 27.011870950756478 29.64342440729072 21.335919716160216 40-41 21.881997987436254 26.913904152583235 29.79297321640823 21.41112464357228 42-43 21.533180346014984 27.344702884484967 29.20506875238163 21.917048017118415 44-45 21.80784617161622 27.42200589450256 28.799078937399784 21.97106899648143 46-47 21.928679829213106 27.333205830996484 28.390216762252024 22.347897577538394 48-49 22.061799695189197 27.41737421586725 28.34725580859312 22.173570280350432 50-51 22.073275474513686 27.58468322619136 28.42397721678578 21.918064082509172 52-53 22.03415883393415 27.700594808407526 28.43754337894781 21.827702978710512 54-55 21.583728944859367 27.371285555378993 28.69301704274591 22.35196845701573 56-57 21.105544564682845 27.90933141311185 29.01720140194133 21.967922620263973 58-59 20.748832785032018 27.088337228882036 30.07455200349528 22.088277982590665 60-61 21.74967265653253 27.32528587996159 28.873389781895725 22.051651681610156 62-63 22.291984917959088 27.26433442691445 28.45084058038615 21.99284007474031 64-65 21.60320730884879 27.326609212319024 28.91757052316245 22.152612955669735 66-67 21.283296327929172 27.30781321961685 29.192395307247704 22.216495145206274 68-69 21.58817598282915 26.94683744530968 29.456608067203792 22.00837850465738 70-71 21.421230591214307 27.075793571497297 29.223587322660084 22.279388514628316 72-73 20.72787809969584 26.92803425668458 29.72912572472336 22.614961918896217 74-75 21.22038647842022 27.24461708132939 29.079027454125818 22.45596898612457 76-77 19.589511104130864 27.493483703974412 30.02555173319883 22.891453458695896 78-79 20.24039734286601 27.496773505199336 29.429289234439754 22.833539917494903 80-81 21.190626724424895 27.91950486990622 28.255807285906947 22.634061119761935 82-83 21.592211591611203 27.77569102003916 27.85753768320603 22.774559705143613 84-85 21.468619308107183 27.436350559491864 27.72592726196445 23.369102870436507 86-87 21.639479128599522 27.465553809790688 27.99618032270765 22.898786738902132 88-89 21.84935785634222 27.097479903084263 27.965836359675873 23.087325880897648 90-91 20.8254562177459 27.428802523396307 28.69425632360041 23.051484935257385 92-93 19.82305249392864 27.764916869045397 29.28545986674139 23.126570770284577 94-95 19.188318388233313 27.58160303130432 29.353796916283386 23.87628166417898 96-97 19.303157931137594 27.54389462239386 28.753660216007415 24.399287230461134 98-99 19.433958369415052 27.46710857543328 28.700499236745756 24.398433818405906 100-101 19.907870668272036 27.418056818013888 28.582184638882925 24.09188787483115 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 538.0 1 407.0 2 295.0 3 304.5 4 342.5 5 429.0 6 537.5 7 753.5 8 1037.0 9 1252.5 10 1411.0 11 1559.5 12 1741.5 13 1974.5 14 2242.0 15 2520.5 16 2824.0 17 3121.5 18 3385.0 19 3733.5 20 4219.5 21 4803.0 22 5577.0 23 6573.0 24 7917.5 25 9752.0 26 12196.0 27 15068.5 28 18060.5 29 21522.0 30 25485.5 31 30078.5 32 35208.5 33 40822.5 34 46569.0 35 51639.5 36 56748.5 37 61398.5 38 64372.0 39 65858.0 40 66770.5 41 68200.5 42 70147.0 43 72715.0 44 75270.0 45 77375.5 46 80156.5 47 83756.5 48 87453.5 49 85369.0 50 78614.0 51 73054.5 52 67774.5 53 63020.5 54 57852.0 55 52152.5 56 46662.0 57 40776.5 58 35147.5 59 29551.0 60 23873.5 61 18620.0 62 13998.0 63 10308.0 64 7406.0 65 5205.0 66 3521.5 67 2386.0 68 1556.5 69 944.0 70 600.0 71 394.0 72 266.5 73 179.5 74 113.0 75 70.0 76 41.0 77 20.5 78 11.5 79 7.0 80 5.0 81 1.5 82 0.5 83 1.0 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023519347888865623 2 0.006897023958971143 3 3.4733214181869064E-4 4 0.0 5 0.14444055211917264 6 0.0 7 0.0 8 0.0015381851994827726 9 0.0 10-11 0.0 12-13 0.0 14-15 7.690925997413863E-4 16-17 0.0 18-19 7.442831610400514E-5 20-21 0.0 22-23 2.9771326441602056E-4 24-25 0.0 26-27 2.480943870133505E-4 28-29 0.0034733214181869067 30-31 0.012379909911966188 32-33 0.003076370398965545 34-35 0.005085934933773684 36-37 0.11231232900094375 38-39 0.11186575910431971 40-41 0.12436971620979258 42-43 0.12697470727343277 44-45 0.12253381774589378 46-47 0.1154631277160133 48-49 0.1146196068001679 50-51 0.11417303690354388 52-53 0.09211744589805702 54-55 0.07948944159907749 56-57 0.5669701026416097 58-59 1.5935350572254512 60-61 0.5253150550620682 62-63 0.005830218094813736 64-65 0.1220376289718671 66-67 1.9052904639464276 68-69 2.8824350166372095 70-71 2.786869058759667 72-73 2.6414113196537397 74-75 2.0097630103177493 76-77 1.4292221447065092 78-79 0.9427834800894331 80-81 0.05475443121384645 82-83 3.969510192213607E-4 84-85 3.2252270311735557E-4 86-87 4.2176045792269583E-4 88-89 1.4885663220801028E-4 90-91 0.25084823470919865 92-93 0.396305973815126 94-95 2.016238273818798 96-97 2.428744811105896 98-99 1.4453978987397798 100-101 0.9500278361902229 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2015362.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.92357220538463 #Duplication Level Percentage of deduplicated Percentage of total 1 74.43600025895026 37.16111033607732 2 13.733231358062433 13.712239346349648 3 4.8675384856444355 7.290147271516756 4 2.254582627591556 4.502272744062915 5 1.2036443509282722 3.0045112831585454 6 0.7609369305668857 2.2793213878139804 7 0.5038093394124143 1.760637335373196 8 0.3547452260590915 1.4168119126141239 9 0.2739016557233535 1.2306734178011318 >10 1.4187464791836386 13.104127431918247 >50 0.11943569309825473 4.146158914220558 >100 0.06765824593860642 6.137191586785682 >500 0.003481503610807669 1.1604985101133816 >1k 0.0018899591030098759 1.76853239080268 >5k 3.978861269494476E-4 1.3257661313918834 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8688 0.4310888068743977 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6507 0.3228700352591743 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6237 0.30947293836045336 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5265 0.26124338952505805 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4759 0.236136237559307 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3366 0.16701714133738754 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3344 0.1659255260345288 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2515 0.12479147666771528 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2373 0.11774559607653613 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2167 0.1075241073315861 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.961887740267009E-5 0.0 8 0.0 0.0 0.0 4.961887740267009E-5 0.0 9 0.0 0.0 0.0 9.923775480534018E-5 0.0 10-11 0.0 0.0 0.0 9.923775480534018E-5 0.0 12-13 0.0 0.0 0.0 2.232849483120154E-4 0.0 14-15 0.0 0.0 0.0 3.4733214181869064E-4 0.0 16-17 0.0 0.0 0.0 3.969510192213607E-4 0.0 18-19 0.0 0.0 0.0 4.7137933532536585E-4 0.0 20-21 0.0 0.0 0.0 7.939020384427214E-4 0.0 22-23 0.0 0.0 0.0 0.0012900908124694223 0.0 24-25 0.0 0.0 0.0 0.002009564534808139 0.0 26-27 0.0 0.0 0.0 0.0030763703989655456 0.0 28-29 0.0 0.0 0.0 0.005855027533515071 0.0 30-31 0.0 0.0 0.0 0.013545953530928936 0.0 32-33 0.0 0.0 0.0 0.029920183073810065 0.0 34-35 0.0 0.0 0.0 0.056218188097225216 0.0 36-37 0.0 0.0 0.0 0.09494572191000922 0.0 38-39 2.4809438701335044E-5 0.0 0.0 0.15203224036178115 0.0 40-41 4.961887740267009E-5 0.0 0.0 0.2141550748699241 0.0 42-43 4.961887740267009E-5 0.0 0.0 0.27632752825546975 0.0 44-45 4.961887740267009E-5 0.0 0.0 0.3509047009916829 0.0 46-47 4.961887740267009E-5 0.0 0.0 0.4367949777757048 0.0 48-49 4.961887740267009E-5 0.0 0.0 0.5213703543085559 0.0 50-51 4.961887740267009E-5 0.0 0.0 0.6028445510037403 0.0 52-53 4.961887740267009E-5 0.0 0.0 0.6793320505199563 0.0 54-55 4.961887740267009E-5 0.0 0.0 0.7639322364915087 0.0 56-57 4.961887740267009E-5 0.0 0.0 0.8317860513396601 0.0 58-59 4.961887740267009E-5 0.0 0.0 0.8809087399683035 0.0 60-61 4.961887740267009E-5 0.0 0.0 0.9234073084636905 0.0 62-63 9.923775480534018E-5 0.0 0.0 0.9618619384507597 0.0 64-65 9.923775480534018E-5 0.0 0.0 0.9970417225292528 0.0 66-67 9.923775480534018E-5 0.0 0.0 1.0272348094287775 0.0 68-69 9.923775480534018E-5 0.0 0.0 1.0706017082787112 0.0 70-71 9.923775480534018E-5 2.4809438701335044E-5 0.0 1.1365700057855612 0.0 72-73 9.923775480534018E-5 4.961887740267009E-5 0.0 1.2344432414623279 0.0 74-75 9.923775480534018E-5 4.961887740267009E-5 0.0 1.3559102533440641 0.0 76-77 9.923775480534018E-5 4.961887740267009E-5 0.0 1.5551796649931875 2.4809438701335044E-5 78-79 9.923775480534018E-5 4.961887740267009E-5 0.0 1.852818501093104 4.961887740267009E-5 80-81 9.923775480534018E-5 4.961887740267009E-5 0.0 2.22329784922014 4.961887740267009E-5 82-83 9.923775480534018E-5 4.961887740267009E-5 0.0 2.5957123335658805 4.961887740267009E-5 84-85 9.923775480534018E-5 7.442831610400514E-5 0.0 2.8890095178930633 4.961887740267009E-5 86-87 1.4885663220801028E-4 9.923775480534018E-5 0.0 3.1126666077855987 4.961887740267009E-5 88-89 1.4885663220801028E-4 9.923775480534018E-5 0.0 3.322380793127984 4.961887740267009E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10035 0.0 26.082922 1 TACATGG 10150 0.0 24.948837 2 ACATGGG 9925 0.0 24.375027 3 GAGTACT 6870 0.0 21.005003 12-13 CATGGGA 6905 0.0 20.585232 4 CATGGGG 3225 0.0 20.57794 4 AGTACTT 7180 0.0 19.67257 12-13 GTATCAA 18320 0.0 19.426527 1 GTACTTT 7545 0.0 19.12559 14-15 GTATAGG 765 0.0 19.076517 1 GGTATCA 14225 0.0 17.837555 1 ATCAACG 20405 0.0 17.276579 3 TCAACGC 20415 0.0 17.245062 4 AACGCAG 20525 0.0 17.245018 6 AGTACAT 7605 0.0 17.20599 2 CAACGCA 20640 0.0 17.102676 5 TATCAAC 21215 0.0 16.662148 2 CATTCCG 540 0.0 16.538683 9 ACTTTTT 8830 0.0 16.5018 16-17 ATGGGAT 2750 0.0 16.43051 5 >>END_MODULE