FastQCFastQC Report
Fri 27 May 2016
SRR939576_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939576_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901793
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34030.1789364036990356No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25020.13156005937554718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22390.11773100437324147No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19260.10127285146175213No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG80650.032.6965751
TACATGG95750.026.0556552
ACATGGG93850.026.0293373
CATGGGG29550.022.8627344
CATGGGA65900.022.510954
GAGTACT62850.022.30725312-13
GTATCAA176000.021.64821
GTACTTT68700.020.34088714-15
AGTACTT68450.020.20640812-13
ATGGGAT27400.019.8105475
GTGCTAG7609.640644E-1118.4699341
GTATAGG5553.6868914E-718.065831
TATCAAC205900.017.7160872
GCTTATA14250.017.5904141
GTCTAAG5704.876747E-717.5904121
ATGGGAG26000.017.427985
GTATTAG5254.339612E-617.1883471
ACTTTTT87000.017.09556416-17
ATCAACG217050.016.8495393
TCAACGC218700.016.8066224
Produced by FastQC (version 0.11.5)