FastQCFastQC Report
Fri 27 May 2016
SRR939576_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939576_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901793
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83060.4367457446735791No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61080.3211706005858682No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA56920.2992965059814607No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA48580.2554431528562783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44230.23257000104638095No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32130.16894583164413793No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT29520.15522194055819954No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA23440.12325210998252702No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG22470.11815166003871083No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT20620.10842399777473152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG92750.026.5046121
TACATGG93150.025.9301362
ACATGGG92150.024.9296973
CATGGGA65500.023.4779244
GAGTACT68100.021.63163412-13
AGTACTT70500.020.86180512-13
TACCGAG1605.295094E-520.6225157
GTACTTT73150.020.00938214-15
ATGGGAT26350.019.8712775
GTATCAA185350.018.4307771
CATGGGG29850.018.173434
ATGGGAG26800.017.777475
GTATAGG6800.017.3471761
TACGCGG1153.5017776E-516.91084764-65
ACTTTTT88800.016.82712416-17
TACTTTT87000.016.71561214-15
ATCAACG205000.016.5446823
TCAACGC205750.016.4843714
AGTACAT69650.016.3235112
AACGCAG208500.016.2775866
Produced by FastQC (version 0.11.5)