##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939576_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1901793 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.498177772239146 31.0 31.0 34.0 30.0 34.0 2 31.69544792729808 31.0 31.0 34.0 30.0 34.0 3 31.747804834700727 31.0 31.0 34.0 30.0 34.0 4 35.54109253741075 37.0 35.0 37.0 33.0 37.0 5 35.29589182418907 37.0 35.0 37.0 33.0 37.0 6 35.313917971093595 37.0 35.0 37.0 32.0 37.0 7 35.22402280374362 37.0 35.0 37.0 32.0 37.0 8 35.188535765985044 37.0 35.0 37.0 32.0 37.0 9 36.78175016944536 39.0 37.0 39.0 32.0 39.0 10-11 36.66514152696955 39.0 35.0 39.0 32.0 39.0 12-13 36.60145241884895 39.0 35.0 39.0 32.0 39.0 14-15 37.64811575181947 39.0 36.0 41.0 32.0 41.0 16-17 37.60808116340738 39.0 36.0 41.0 32.0 41.0 18-19 37.60857070143806 39.0 36.0 41.0 32.0 41.0 20-21 37.55989321655932 39.0 36.0 41.0 32.0 41.0 22-23 37.42137130592026 39.0 36.0 41.0 32.0 41.0 24-25 37.291871144756556 39.0 36.0 41.0 31.0 41.0 26-27 36.996254850028365 39.0 36.0 40.0 31.0 41.0 28-29 36.7948110020386 39.0 36.0 40.0 30.0 41.0 30-31 36.55621773768228 38.0 35.0 40.0 30.0 41.0 32-33 36.391338068864485 38.0 35.0 40.0 30.0 41.0 34-35 36.36177175959739 38.0 35.0 40.0 30.0 41.0 36-37 36.26578786439954 38.0 35.0 40.0 30.0 41.0 38-39 36.298887418346794 38.0 35.0 40.0 30.0 41.0 40-41 36.18806384291035 38.0 35.0 40.0 30.0 41.0 42-43 36.016929287256815 38.0 35.0 40.0 29.5 41.0 44-45 35.79631905259931 38.0 35.0 40.0 28.5 41.0 46-47 35.479019535774924 38.0 34.5 40.0 27.0 41.0 48-49 35.174955423644946 38.0 34.0 40.0 26.5 41.0 50-51 34.937258155856085 38.0 34.0 40.0 26.0 41.0 52-53 34.69691759302931 38.0 33.5 40.0 26.0 41.0 54-55 34.42542642653538 38.0 33.0 40.0 24.5 41.0 56-57 33.60358409143372 37.5 32.5 40.0 21.0 41.0 58-59 30.397772785997212 35.5 26.5 39.0 8.0 40.5 60-61 30.136648941288563 34.5 25.5 39.0 10.5 40.0 62-63 32.05487190246257 35.5 30.5 39.0 18.0 40.0 64-65 32.115865396496886 36.0 31.0 39.0 15.5 40.0 66-67 27.089459525826417 31.5 18.5 37.0 2.0 39.5 68-69 19.520023998405712 19.0 8.0 32.0 2.0 37.0 70-71 17.606705356471497 15.5 7.5 29.0 2.0 35.0 72-73 19.2978391970104 19.5 7.5 30.5 2.0 35.5 74-75 20.741693759520622 22.5 9.0 32.5 2.0 36.0 76-77 22.577926724937996 27.0 11.0 33.0 2.0 35.5 78-79 24.790724069338776 30.5 13.0 34.5 2.0 36.5 80-81 26.444309133538717 32.0 22.5 35.0 2.0 36.5 82-83 27.644126621561863 33.0 26.5 35.0 2.0 36.0 84-85 27.719824660202242 34.0 27.0 35.0 2.0 36.0 86-87 27.44941747077626 33.5 26.5 35.0 2.0 36.0 88-89 27.005149351164928 33.0 26.0 35.0 2.0 35.5 90-91 25.12471020768296 32.0 16.0 34.5 2.0 35.0 92-93 23.451882775885704 31.0 2.0 34.0 2.0 35.0 94-95 19.604511900085868 27.0 2.0 34.0 2.0 35.0 96-97 16.90839802228739 16.5 2.0 33.0 2.0 35.0 98-99 17.180550669815275 19.5 2.0 33.0 2.0 35.0 100-101 16.40731351939985 9.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2417501783591085 1101 2 0.25555168786166504 1101 3 0.19656962844604564 1101 4 0.18661779582733828 1101 5 0.32241650334614746 1101 6 0.20541041841863716 1101 7 0.3032080907098802 1101 8 0.3770857861458978 1101 9 0.4105057399158696 1101 10-11 0.2882214108724881 1101 12-13 0.2902448938553803 1101 14-15 0.48356599427017244 1101 16-17 0.3692680916168385 1101 18-19 0.34331849661165137 1101 20-21 0.3594159378932673 1101 22-23 0.350082123328761 1101 24-25 0.38765897526007365 1101 26-27 0.4736571739646749 1101 28-29 0.2503309610868243 1101 30-31 0.3850513125196784 1101 32-33 0.42767561703545454 1101 34-35 0.23932068614826818 1101 36-37 0.19705947417266856 1101 38-39 0.22969815418083073 1101 40-41 0.28720535344955067 1101 42-43 0.3489641247225421 1101 44-45 0.2739788069333926 1101 46-47 0.27252663570686764 1101 48-49 0.1665514382718598 1101 50-51 0.086185507790276 1101 52-53 0.0651447058964223 1101 54-55 -0.04663550430449703 1101 56-57 -3.6569392562466447 1101 58-59 -10.196015982824957 1101 60-61 -11.75970976975275 1101 62-63 -4.118607590449489 1101 64-65 -1.0765802789890984 1101 66-67 -2.3609917081445353 1101 68-69 -4.691531568818053 1101 70-71 -4.593058652257952 1101 72-73 -3.295602652636944 1101 74-75 -6.0303601011119845 1101 76-77 -4.9858871167578265 1101 78-79 1.3381898526307374 1101 80-81 1.9069564748977506 1101 82-83 0.4425479370530532 1101 84-85 0.3226929843510611 1101 86-87 0.2956524633899775 1101 88-89 -0.004712486667511229 1101 90-91 1.446839863583783 1101 92-93 1.1374855381724984 1101 94-95 -8.731225383134241 1101 96-97 -9.661939169634397 1101 98-99 -8.729061766442 1101 100-101 -9.869896421102874 1102 1 0.29415570561085147 1102 2 0.33680579175803516 1102 3 0.35980986363150436 1102 4 0.23194026724173966 1102 5 0.37839177646308286 1102 6 0.34123803088530025 1102 7 0.4013833613914102 1102 8 0.3694538268509291 1102 9 0.41251729311594687 1102 10-11 0.3564319153484874 1102 12-13 0.3498571207267318 1102 14-15 0.459863504627684 1102 16-17 0.48277413751677756 1102 18-19 0.4319654683225238 1102 20-21 0.49504988860248034 1102 22-23 0.43699234482910043 1102 24-25 0.4602567264419477 1102 26-27 0.26158373330478213 1102 28-29 0.32033874970841225 1102 30-31 0.25532498210790067 1102 32-33 0.27716003651384824 1102 34-35 0.3550506809121501 1102 36-37 0.19397680987396626 1102 38-39 0.4554287296958037 1102 40-41 0.29200170183651863 1102 42-43 0.43901379767536497 1102 44-45 0.3163275048800642 1102 46-47 0.3703745313739901 1102 48-49 0.5426876496952318 1102 50-51 0.5462053610475834 1102 52-53 0.3657908851630438 1102 54-55 0.17311766711167564 1102 56-57 -3.1610406937929483 1102 58-59 -9.598988046823724 1102 60-61 -7.906108105994523 1102 62-63 -2.166165016897832 1102 64-65 -0.38640572113270366 1102 66-67 -1.8340230790074976 1102 68-69 -5.0577319017195705 1102 70-71 -4.869493716323229 1102 72-73 -3.60199803360498 1102 74-75 -4.3186628607100666 1102 76-77 -4.182813140308223 1102 78-79 1.843875124876547 1102 80-81 2.2788719372084074 1102 82-83 0.8320719834269674 1102 84-85 0.46024634085823735 1102 86-87 0.414988969310361 1102 88-89 0.38215111082326914 1102 90-91 1.6224102876163897 1102 92-93 1.4897266519781702 1102 94-95 -8.262286668298955 1102 96-97 -9.450118051276387 1102 98-99 -8.421685080039161 1102 100-101 -9.173554895432272 1103 1 0.18324855568636167 1103 2 0.20272331952527267 1103 3 0.25385729669628176 1103 4 0.25564192100119953 1103 5 0.14900332525382254 1103 6 0.2672289474831544 1103 7 0.31187446769176574 1103 8 0.31262583866455884 1103 9 0.32507187908093726 1103 10-11 0.25780521267554235 1103 12-13 0.22431126107682786 1103 14-15 0.4183456242369701 1103 16-17 0.3965892222133647 1103 18-19 0.3080478884800826 1103 20-21 0.22903444086738034 1103 22-23 0.23767824956124173 1103 24-25 0.3287835935237311 1103 26-27 0.43151718864286437 1103 28-29 0.3427929235354128 1103 30-31 0.3158154374682951 1103 32-33 0.34364657879519456 1103 34-35 0.2657338144999031 1103 36-37 0.2593056089455317 1103 38-39 0.3465887790928548 1103 40-41 0.2282579415609618 1103 42-43 0.16673863233661734 1103 44-45 0.3489061198286194 1103 46-47 0.21806574033687554 1103 48-49 0.15895343033962916 1103 50-51 0.06138156119488514 1103 52-53 0.2505521947798499 1103 54-55 0.28311489596728734 1103 56-57 0.48723319942313736 1103 58-59 -8.553312519934327 1103 60-61 -7.521276122896644 1103 62-63 -2.116548513524105 1103 64-65 -0.2833716899917391 1103 66-67 -1.627037656342889 1103 68-69 -3.3263613383403374 1103 70-71 -3.971505306774125 1103 72-73 -3.1723465096615513 1103 74-75 -3.791530288418535 1103 76-77 -0.37335349486591696 1103 78-79 3.37605155192292 1103 80-81 2.6394536171542526 1103 82-83 1.159380867455507 1103 84-85 0.7610670831529696 1103 86-87 0.6249970781623979 1103 88-89 0.5781830738350031 1103 90-91 1.9534127909454817 1103 92-93 1.5895478294727567 1103 94-95 -6.886116371247155 1103 96-97 -8.694742217143329 1103 98-99 -7.906637208054875 1103 100-101 -9.227050071037272 1104 1 0.1997161258452067 1104 2 0.20907195003497847 1104 3 0.25234787740162545 1104 4 0.1678105613577543 1104 5 0.34638419125884923 1104 6 0.1376747768215978 1104 7 0.17734504373281368 1104 8 0.21287136223222802 1104 9 0.171370238802119 1104 10-11 0.12399486829473005 1104 12-13 0.2884129946416465 1104 14-15 0.36869026417893025 1104 16-17 0.30081721578699927 1104 18-19 0.3425086435784266 1104 20-21 0.19313714616135513 1104 22-23 0.3613437415153271 1104 24-25 0.29955721949869485 1104 26-27 0.14928493241732355 1104 28-29 0.2902272346442274 1104 30-31 0.24269767685053267 1104 32-33 0.355587510618669 1104 34-35 0.28825363777476554 1104 36-37 0.15770970575567134 1104 38-39 0.07673311326403365 1104 40-41 0.057194740296417024 1104 42-43 0.06503745848780085 1104 44-45 0.19755606411614934 1104 46-47 -2.1054169779688436E-4 1104 48-49 -0.03653418335267844 1104 50-51 0.11841882376906199 1104 52-53 0.03042962734183874 1104 54-55 0.09448121194770209 1104 56-57 0.5933654582399583 1104 58-59 -8.290670323567298 1104 60-61 -7.727622809948027 1104 62-63 -2.207878098142281 1104 64-65 -0.42366184831880815 1104 66-67 -1.9277117124699608 1104 68-69 -3.8030576535433624 1104 70-71 -3.8792396230654873 1104 72-73 -3.004128855286176 1104 74-75 -3.9023381840418665 1104 76-77 -0.30110219274884287 1104 78-79 3.569942290696204 1104 80-81 2.8919768738721388 1104 82-83 1.2344161736149708 1104 84-85 1.0187307871253957 1104 86-87 0.9417905014994439 1104 88-89 0.9021915861824397 1104 90-91 2.5762247807876726 1104 92-93 2.288109346406756 1104 94-95 -6.115024989836982 1104 96-97 -7.380653439967304 1104 98-99 -5.091003381515007 1104 100-101 -4.529089406633656 1105 1 0.2581264938416936 1105 2 0.3112697449522521 1105 3 0.22843203361060205 1105 4 0.1928844095763651 1105 5 0.2924288513298947 1105 6 0.30281983734354156 1105 7 0.15741477954500738 1105 8 0.254525448752716 1105 9 0.22849151645911547 1105 10-11 0.2586127646923799 1105 12-13 0.20344163951618555 1105 14-15 0.2873249547167944 1105 16-17 0.41851485501017294 1105 18-19 0.45123234003077783 1105 20-21 0.33041509277862957 1105 22-23 0.39874700058557266 1105 24-25 0.3815774852860301 1105 26-27 0.3293411337274179 1105 28-29 0.3823146145255265 1105 30-31 0.4229966473765572 1105 32-33 0.4035181041775715 1105 34-35 0.39290515273984994 1105 36-37 0.4490438858629844 1105 38-39 0.35858533353758304 1105 40-41 0.3221283597156841 1105 42-43 0.41930660010695675 1105 44-45 0.26488926557484405 1105 46-47 0.2068514870199607 1105 48-49 0.1267368691867361 1105 50-51 0.2815526383501634 1105 52-53 0.5073594533146419 1105 54-55 0.52907927982654 1105 56-57 0.851320527478876 1105 58-59 -8.258787579678884 1105 60-61 -7.526839417823215 1105 62-63 -1.8383923429691187 1105 64-65 -0.1526537929238927 1105 66-67 -1.5308777448448723 1105 68-69 -3.1881625080758624 1105 70-71 -3.8746023997774373 1105 72-73 -3.0068724313666415 1105 74-75 -3.6643027312181253 1105 76-77 -0.14986406974766808 1105 78-79 3.778040702616412 1105 80-81 3.115898349151795 1105 82-83 1.6383652195272198 1105 84-85 1.3714048924048932 1105 86-87 1.3049001338058268 1105 88-89 1.4018835106277372 1105 90-91 2.7787354782499065 1105 92-93 2.3243113001074036 1105 94-95 -6.387380106777918 1105 96-97 -7.580431918973208 1105 98-99 -5.743506459904383 1105 100-101 -4.803749884657126 1106 1 0.08625448844839312 1106 2 0.19591665223080312 1106 3 0.16567856046876273 1106 4 0.16180069301363176 1106 5 0.18990218578683482 1106 6 0.19062616002671007 1106 7 0.18991712707588704 1106 8 0.038059024463599656 1106 9 0.013144202275213956 1106 10-11 0.07152731456837813 1106 12-13 0.1228563218170109 1106 14-15 0.11551579091933206 1106 16-17 0.22290172809903908 1106 18-19 0.19005231450890392 1106 20-21 0.17336888855548693 1106 22-23 0.15718149845473306 1106 24-25 0.1441932366162959 1106 26-27 0.08166362427710538 1106 28-29 0.17517426829481053 1106 30-31 0.34370812981220666 1106 32-33 0.2266433299016981 1106 34-35 0.2170411409901547 1106 36-37 0.22500035421345643 1106 38-39 0.2863524103198998 1106 40-41 0.11712318310912906 1106 42-43 0.32584212607426366 1106 44-45 0.21060496152745856 1106 46-47 0.2687673129896666 1106 48-49 0.3179888856555735 1106 50-51 0.2506653920640787 1106 52-53 0.2847983573186852 1106 54-55 0.23638238819820145 1106 56-57 0.84710701051646 1106 58-59 -8.64391097553278 1106 60-61 -7.406758277525139 1106 62-63 -2.098001569367405 1106 64-65 -0.271448486300244 1106 66-67 0.6725279807227516 1106 68-69 -2.3860983663090707 1106 70-71 -2.5861483993440473 1106 72-73 -1.3591703631317422 1106 74-75 -1.1789450638211143 1106 76-77 2.2159929596024845 1106 78-79 4.590266402662159 1106 80-81 3.572805627701385 1106 82-83 1.9030212761568883 1106 84-85 1.4008335395047915 1106 86-87 1.2811544376124395 1106 88-89 1.203813782941321 1106 90-91 2.3810403924145476 1106 92-93 1.8716250024645653 1106 94-95 -6.197159077716691 1106 96-97 -7.652483059030512 1106 98-99 -5.854165694729701 1106 100-101 -5.447078322285037 1107 1 -0.02656169124178831 1107 2 0.060085360293427925 1107 3 0.08748989997953771 1107 4 0.04012165962940628 1107 5 0.11694186579061494 1107 6 -0.02899514623002375 1107 7 0.05180349528454542 1107 8 0.12173259045690088 1107 9 0.07095937606013791 1107 10-11 0.04786593777993886 1107 12-13 0.10531984295877095 1107 14-15 0.1620862457068526 1107 16-17 0.13104837725112617 1107 18-19 0.1364343691591401 1107 20-21 0.23313090320620233 1107 22-23 0.07488874452283767 1107 24-25 0.1371508347512318 1107 26-27 0.3358840477040488 1107 28-29 0.25627709548028577 1107 30-31 0.19055585942486886 1107 32-33 0.15142031478945484 1107 34-35 0.14350674494750848 1107 36-37 0.15126927410789648 1107 38-39 0.12873711582633973 1107 40-41 -0.08392698197767601 1107 42-43 0.013696944360432894 1107 44-45 -0.18033147709456188 1107 46-47 -0.14548636448159868 1107 48-49 -0.05877517630818829 1107 50-51 -0.2639382220943318 1107 52-53 -0.11754469187599881 1107 54-55 -0.3256433866447068 1107 56-57 0.20660275612893741 1107 58-59 -8.621174439870483 1107 60-61 -7.814274283252882 1107 62-63 -2.7542192472647073 1107 64-65 -0.7368062927167358 1107 66-67 0.6117079904267761 1107 68-69 -2.9221791227498244 1107 70-71 -2.6496999549469393 1107 72-73 -1.3290638555164485 1107 74-75 -1.4167608293150131 1107 76-77 1.5798634463422907 1107 78-79 3.5511220138383592 1107 80-81 2.409800929532132 1107 82-83 0.7697551609629834 1107 84-85 0.3274235206884448 1107 86-87 0.23710833164919976 1107 88-89 0.10779077729641529 1107 90-91 1.732558007590697 1107 92-93 1.3818229882872188 1107 94-95 -6.694616504256022 1107 96-97 -8.258150447582127 1107 98-99 -6.089299361786896 1107 100-101 -5.530436270711682 1108 1 -0.11056937024342517 1108 2 -0.11589885685967971 1108 3 -0.1323631640179137 1108 4 -0.1091615103403214 1108 5 -0.08865086358165541 1108 6 -0.14799697895299602 1108 7 -0.09077247943444888 1108 8 -0.0721439531688759 1108 9 -0.1494853341455098 1108 10-11 -0.1620961772113816 1108 12-13 -0.15403174648508156 1108 14-15 -0.2519313478722083 1108 16-17 -0.15453906029148357 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0.3611011207594146 2313 58-59 3.2334210595207686 2313 60-61 3.2929158859156225 2313 62-63 0.4618435870198887 2313 64-65 -0.1906044461169003 2313 66-67 -1.8427592636784205 2313 68-69 -4.664134559816091 2313 70-71 -3.5371052473740665 2313 72-73 -2.9045497537318816 2313 74-75 -3.8840461159027164 2313 76-77 0.3821099947747868 2313 78-79 3.078993217681038 2313 80-81 2.2408674701503806 2313 82-83 0.7764546720293097 2313 84-85 0.5490502255664929 2313 86-87 0.4729258770405629 2313 88-89 0.49156213832166173 2313 90-91 2.1496033391929927 2313 92-93 3.645793905497314 2313 94-95 5.706353402393503 2313 96-97 1.2677558561142384 2313 98-99 2.9735123635503946 2313 100-101 4.1132605762353975 2314 1 -0.4184594871981524 2314 2 -0.345979876236747 2314 3 -0.4435377389340509 2314 4 -0.22626047266589921 2314 5 -0.4059822517905758 2314 6 -0.389930069969985 2314 7 -0.5110388625745088 2314 8 -0.5697166686779482 2314 9 -0.6364093176943726 2314 10-11 -0.49617541630508555 2314 12-13 -0.5975283548804384 2314 14-15 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3.062421058145368 2314 80-81 2.385005164176384 2314 82-83 0.9360826257749082 2314 84-85 0.5226709624039074 2314 86-87 0.4458338328226823 2314 88-89 0.45794369256620726 2314 90-91 2.055081888938112 2314 92-93 3.6292647037484542 2314 94-95 6.286539680085369 2314 96-97 3.4760470271753263 2314 98-99 5.985440309022643 2314 100-101 6.460588601895516 2315 1 -0.4356320851973159 2315 2 -0.35339013188407975 2315 3 -0.5342010304364031 2315 4 -0.2910904823321161 2315 5 -0.44962974853099524 2315 6 -0.5329824117191038 2315 7 -0.6639656961226024 2315 8 -0.5811040336313269 2315 9 -0.6804901537952048 2315 10-11 -0.41842876572079746 2315 12-13 -0.4876455472381025 2315 14-15 -0.6459761221788582 2315 16-17 -0.6909662907025407 2315 18-19 -0.7223042987385639 2315 20-21 -0.7440392316562168 2315 22-23 -0.6355167262513319 2315 24-25 -0.6621915296592888 2315 26-27 -0.6085338910516143 2315 28-29 -0.754018892073816 2315 30-31 -0.666995942630642 2315 32-33 -0.6928385162065993 2315 34-35 -0.6092121128929122 2315 36-37 -0.4572288178506483 2315 38-39 -0.6336736207446592 2315 40-41 -0.49829041472943203 2315 42-43 -0.4921015431955098 2315 44-45 -0.5400475422661657 2315 46-47 -0.6594430821643584 2315 48-49 -0.5393347261237906 2315 50-51 -0.4021300481816752 2315 52-53 -0.3324491351865859 2315 54-55 -0.10267753259828538 2315 56-57 0.3336010825891975 2315 58-59 3.499498230334698 2315 60-61 3.6045538855206196 2315 62-63 0.7698155223088214 2315 64-65 0.0334431331774141 2315 66-67 -1.7847052008655133 2315 68-69 -4.561135932466222 2315 70-71 -3.3180184414132086 2315 72-73 -2.7264216801062204 2315 74-75 -0.3462741038793453 2315 76-77 2.1017516232589273 2315 78-79 3.8578844985699767 2315 80-81 2.8185657717975126 2315 82-83 0.9673421861338163 2315 84-85 0.7243152869171077 2315 86-87 0.6912969299326619 2315 88-89 0.9144152115656219 2315 90-91 2.4074863501973383 2315 92-93 3.8683455818099617 2315 94-95 6.494106944958709 2315 96-97 4.088606544581761 2315 98-99 6.570378638270533 2315 100-101 6.902209092743895 2316 1 -0.3621227338649575 2316 2 -0.4317017344548759 2316 3 -0.5844893827005819 2316 4 -0.31323495232472 2316 5 -0.42306716553110135 2316 6 -0.5207117073095446 2316 7 -0.40786743628449074 2316 8 -0.3312582253565992 2316 9 -0.5176076935520726 2316 10-11 -0.5164596386068965 2316 12-13 -0.5625505397528983 2316 14-15 -0.5574776604326672 2316 16-17 -0.6945726892731727 2316 18-19 -0.6667519705023963 2316 20-21 -0.6695663946514614 2316 22-23 -0.8124216711413652 2316 24-25 -0.6867250882420635 2316 26-27 -0.7120837417531973 2316 28-29 -0.6484814923703937 2316 30-31 -0.8110123224104271 2316 32-33 -0.8181973701348895 2316 34-35 -0.7344772873343217 2316 36-37 -0.6601150357402403 2316 38-39 -0.9751638729753651 2316 40-41 -0.9318758453188138 2316 42-43 -0.8152596618706767 2316 44-45 -0.9309677234182132 2316 46-47 -1.0685139255754308 2316 48-49 -0.9212174535366344 2316 50-51 -0.7957310850408845 2316 52-53 -0.7578204709906444 2316 54-55 -0.7886983027535663 2316 56-57 0.016334042931639203 2316 58-59 3.2392137011356077 2316 60-61 3.1878749855689996 2316 62-63 0.5155695257330954 2316 64-65 -0.17125164832565432 2316 66-67 -2.2072185272958755 2316 68-69 -3.1176552789811467 2316 70-71 -3.3484288177509214 2316 72-73 -0.6795293400789895 2316 74-75 5.532769193052442 2316 76-77 5.620233303816587 2316 78-79 4.744579551691217 2316 80-81 3.055826081185792 2316 82-83 1.1864192547823045 2316 84-85 0.7441939170299321 2316 86-87 0.6924155886098795 2316 88-89 0.8996092733328531 2316 90-91 2.2286205471090064 2316 92-93 3.8082232384934827 2316 94-95 6.335780369370486 2316 96-97 3.475171414617172 2316 98-99 5.913949203129054 2316 100-101 6.200228530452399 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 35.0 5 79.0 6 188.0 7 447.0 8 992.0 9 1699.0 10 2919.0 11 4569.0 12 6620.0 13 9042.0 14 11138.0 15 14956.0 16 19926.0 17 26688.0 18 28351.0 19 27402.0 20 28573.0 21 29281.0 22 29491.0 23 29963.0 24 31724.0 25 33163.0 26 41218.0 27 58824.0 28 83075.0 29 111080.0 30 135200.0 31 150001.0 32 155633.0 33 154760.0 34 157666.0 35 167166.0 36 168701.0 37 128263.0 38 49774.0 39 3175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.81418733057662 20.018439995329516 14.322869275896203 22.84450339819766 2 15.268803406892351 21.776049837054583 39.4844856083725 23.47066114768056 3 18.93194362577171 26.54245392895434 30.72314465679983 23.802457788474126 4 12.42543221055078 17.565791860628366 38.15162848953592 31.857147439284926 5 12.612337676004461 39.102614021425715 34.666722791657016 13.618325510912802 6 30.221375302149077 37.74222536311786 17.991863467790658 14.044535866942406 7 27.147696936522536 34.016741043846515 21.56717371448943 17.268388305141517 8 25.68551134929582 35.054096347024284 20.36321532533526 18.897176978344636 9 26.485952992781026 16.66653521177121 20.749734592566067 36.0977772028817 10-11 24.154074602230633 27.065931991546925 28.22494351383142 20.555049892391022 12-13 25.917752352648264 24.672611582858913 28.215136978630166 21.194499085862656 14-15 22.74816461613371 25.641766511619103 27.138599481959552 24.471469390287638 16-17 21.39704478878616 29.145522146732056 27.670361600868233 21.787071463613547 18-19 21.323626650261005 28.059596720445164 29.811768695947094 20.805007933346744 20-21 22.32829755919808 27.060121685167626 29.300007939875684 21.311572815758602 22-23 21.88934337228079 27.122615342468926 29.06352058294462 21.924520702305667 24-25 21.96458496228029 27.15582311620993 29.437645882223652 21.441946039286126 26-27 21.993347323654387 27.02499906666926 29.569127206932833 21.41252640274352 28-29 21.47253619200885 27.30737448577415 29.801792175853343 21.418297146363656 30-31 21.66474205466529 27.391645921873376 29.592539656998383 21.351072366462954 32-33 21.5639447812241 27.315935660829 29.333186795619852 21.786932762327048 34-35 22.036191909456075 27.050991428425696 29.194489709746442 21.71832695237179 36-37 22.215994079931452 26.78078377749637 29.40384046552118 21.599381677050996 38-39 22.04441333505254 27.006007562739953 29.602811423579222 21.346767678628286 40-41 21.829951804730474 27.005666904739474 29.76393423295731 21.40044705757274 42-43 21.50563017808085 27.39326978773306 29.1970532063474 21.904046827838688 44-45 21.817186042643502 27.405934381980835 28.820234714008098 21.956644861367565 46-47 21.928487027187774 27.34898825486135 28.375839173445407 22.34668554450547 48-49 22.10105847603134 27.456223562768482 28.353763872351344 22.088954088848833 50-51 22.11366014048722 27.6074019967962 28.37343520095314 21.90550266176344 52-53 22.028920535221694 27.683507907329897 28.431607009479432 21.855964547968977 54-55 21.594907592523224 27.400363653199435 28.6093053550459 22.395423399231447 56-57 21.870721961258845 27.99202559765262 28.110347121198124 22.026905319890407 58-59 20.957991058411125 27.146231605743782 29.73635793263431 22.159419403210784 60-61 21.22324183223788 27.325179091267625 29.441087513668446 22.010491562826047 62-63 22.283061227333594 27.25787245946542 28.441893097402087 22.017173215798895 64-65 21.96343738916467 27.352311803093095 28.536772548884542 22.14747825885769 66-67 21.545528791602376 27.36613917421076 28.86479235102264 22.22353968316423 68-69 21.06822960020833 26.89772252023034 30.09130008706403 21.942747792497304 70-71 21.881364281157555 27.087564971857862 28.798730575736126 22.232340171248456 72-73 21.225872874128598 26.98500125179234 29.196643857716058 22.592482016363 74-75 21.27250409928076 27.30247485503713 28.991695444412102 22.433325601270013 76-77 20.327991038994096 27.419110049638867 29.511379910681296 22.74151900068574 78-79 21.37810130825743 27.287361901166342 28.688548861104323 22.645987929471907 80-81 21.27279991309935 27.90483958229825 28.173642433817818 22.648718070784575 82-83 21.59654283686873 27.80235257248367 27.83298160338902 22.768122987258586 84-85 21.472446027182606 27.455275158503866 27.768667831372657 23.30361098294087 86-87 21.692115805984642 27.465141628811523 27.956316415207542 22.886426149996293 88-89 21.8146530861312 27.141974891556142 27.986126757915763 23.057245264396894 90-91 21.263304056429853 27.352683680981727 28.399018423162175 22.98499383942625 92-93 21.02573114214514 27.63964983991201 28.33240427669455 23.0022147412483 94-95 20.120975213008645 27.471598404976827 28.71800888613295 23.689417495881575 96-97 19.92531934960254 27.371944712366147 28.511482256929664 24.191253681101646 98-99 20.498647869137017 27.12136366763512 28.250564931387956 24.1294235318399 100-101 19.837873084404148 27.2981332955388 28.869532705140234 23.994460914916818 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 625.0 1 455.0 2 304.5 3 340.0 4 363.5 5 401.0 6 529.0 7 792.0 8 1130.0 9 1421.5 10 1555.5 11 1624.0 12 1772.0 13 1930.0 14 2127.5 15 2318.0 16 2540.0 17 2853.0 18 3134.0 19 3404.5 20 3755.5 21 4326.0 22 5104.5 23 5972.5 24 7061.0 25 8791.0 26 11312.5 27 14074.0 28 16682.0 29 19637.5 30 23351.5 31 27785.0 32 32642.5 33 37653.0 34 43106.0 35 48075.5 36 53023.5 37 57609.5 38 59885.0 39 60936.0 40 61724.5 41 63023.0 42 65036.0 43 66931.5 44 69035.5 45 71346.5 46 74004.5 47 78070.0 48 84052.5 49 82797.0 50 74704.0 51 68834.5 52 63813.5 53 59596.0 54 55520.0 55 50460.5 56 45046.0 57 39718.0 58 34987.0 59 30046.0 60 24578.5 61 19216.5 62 14392.5 63 10634.0 64 7763.0 65 5514.5 66 3758.5 67 2594.5 68 1686.0 69 996.5 70 627.5 71 417.5 72 287.0 73 187.5 74 126.0 75 81.0 76 43.0 77 24.0 78 13.0 79 4.0 80 2.5 81 1.5 82 0.0 83 0.5 84 2.0 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025817741468182918 2 0.012409342131346576 3 1.0516391636734387E-4 4 0.0 5 0.06693683276781438 6 0.0 7 0.0 8 5.2581958183671936E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 5.784015400203913E-4 16-17 0.0 18-19 5.521105609285553E-4 20-21 0.0 22-23 0.0 24-25 1.0516391636734387E-4 26-27 1.0516391636734387E-4 28-29 0.0013934218918673063 30-31 0.005836597358387584 32-33 0.006362416940224305 34-35 0.0022084422437142212 36-37 0.09543625410336457 38-39 0.08875834541403822 40-41 0.09538367214518088 42-43 0.0997742656535175 44-45 0.10248223649997659 46-47 0.09769727830526245 48-49 0.08704943177306888 50-51 0.08144945322650783 52-53 0.06885607424151839 54-55 0.05684109679654937 56-57 0.07690111384362021 58-59 0.7294957968611726 60-61 0.33970574084561256 62-63 0.0027605528046427763 64-65 0.002182151264622385 66-67 1.1079544408881514 68-69 2.3721561705190837 70-71 2.3271723052929527 72-73 1.8125263895702632 74-75 1.2792927516296464 76-77 0.739223459125152 78-79 0.5560542077923307 80-81 0.0405669807387029 82-83 1.3145489545917985E-4 84-85 0.0013408399336836345 86-87 0.0013671309127754703 88-89 1.3145489545917985E-4 90-91 0.3297677507488985 92-93 0.05886550218662073 94-95 0.9561503276118904 96-97 1.6525983637546253 98-99 0.6406059965516752 100-101 0.5714081395819629 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1901793.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.648293039082134 #Duplication Level Percentage of deduplicated Percentage of total 1 74.86886018342076 37.91979970071965 2 13.51774815003745 13.693017390632148 3 4.843118397777822 7.358870395008632 4 2.1634819745340717 4.383066761238949 5 1.201818068699716 3.0435016861583484 6 0.7614150928886759 2.313862484940336 7 0.49338784975158617 1.7492476677298678 8 0.33934619985291714 1.3749844617479585 9 0.2481907584678721 1.1313394438025557 >10 1.3784240171082893 12.992034626458773 >50 0.11413352162554243 4.013543933666671 >100 0.06508594321097316 6.061497289550843 >500 0.0028067864762294534 0.9895842042477291 >1k 0.0018711909841529688 1.917039684844896 >5k 3.1186516402549483E-4 1.0586102692527184 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8306 0.4367457446735791 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6108 0.3211706005858682 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5692 0.2992965059814607 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4858 0.2554431528562783 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4423 0.23257000104638095 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3213 0.16894583164413793 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2952 0.15522194055819954 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2344 0.12325210998252702 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2247 0.11815166003871083 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2062 0.10842399777473152 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.2581958183671936E-5 0.0 4 0.0 0.0 0.0 5.2581958183671936E-5 0.0 5 0.0 0.0 0.0 5.2581958183671936E-5 0.0 6 0.0 0.0 0.0 5.2581958183671936E-5 0.0 7 0.0 0.0 0.0 5.2581958183671936E-5 0.0 8 0.0 0.0 0.0 5.2581958183671936E-5 0.0 9 0.0 0.0 0.0 5.2581958183671936E-5 0.0 10-11 0.0 0.0 0.0 1.0516391636734387E-4 0.0 12-13 0.0 0.0 0.0 2.6290979091835964E-4 0.0 14-15 0.0 0.0 0.0 3.154917491020316E-4 0.0 16-17 0.0 0.0 0.0 3.6807370728570354E-4 0.0 18-19 0.0 0.0 0.0 4.206556654693755E-4 0.0 20-21 0.0 0.0 0.0 5.521105609285553E-4 0.0 22-23 0.0 0.0 0.0 8.938932891224229E-4 0.0 24-25 0.0 0.0 0.0 0.0015248767873264862 0.0 26-27 0.0 0.0 0.0 0.0021295693064387134 0.0 28-29 0.0 0.0 0.0 0.004522048403795787 0.0 30-31 0.0 0.0 0.0 0.011936104507693529 0.0 32-33 0.0 0.0 0.0 0.027421491192784912 0.0 34-35 0.0 0.0 0.0 0.053212941681876 0.0 36-37 0.0 0.0 0.0 0.09159777115595652 0.0 38-39 0.0 0.0 0.0 0.14759755662156712 0.0 40-41 0.0 0.0 0.0 0.21019637783922857 0.0 42-43 0.0 0.0 0.0 0.27453040367695114 0.0 44-45 0.0 0.0 0.0 0.34767190751043886 0.0 46-47 0.0 0.0 0.0 0.4383494943981811 0.0 48-49 0.0 0.0 0.0 0.5295791918468519 0.0 50-51 0.0 0.0 0.0 0.6224126390201248 0.0 52-53 0.0 0.0 0.0 0.7120648777232853 0.0 54-55 0.0 0.0 0.0 0.8108663771504049 0.0 56-57 0.0 0.0 0.0 0.9093523848284224 0.0 58-59 0.0 0.0 0.0 0.9806535201254816 0.0 60-61 0.0 0.0 0.0 1.0468279144996329 0.0 62-63 0.0 0.0 2.6290979091835968E-5 1.1040370850034678 0.0 64-65 0.0 5.2581958183671936E-5 5.2581958183671936E-5 1.1657157219529148 0.0 66-67 0.0 5.2581958183671936E-5 5.2581958183671936E-5 1.2162995657256075 0.0 68-69 0.0 5.2581958183671936E-5 5.2581958183671936E-5 1.277741583863228 0.0 70-71 0.0 5.2581958183671936E-5 5.2581958183671936E-5 1.3749130425866538 0.0 72-73 0.0 5.2581958183671936E-5 5.2581958183671936E-5 1.5098909292441398 5.2581958183671936E-5 74-75 0.0 5.2581958183671936E-5 5.2581958183671936E-5 1.6934019633051545 5.2581958183671936E-5 76-77 0.0 5.2581958183671936E-5 5.2581958183671936E-5 1.9273128042852192 5.2581958183671936E-5 78-79 0.0 5.2581958183671936E-5 5.2581958183671936E-5 2.219353000037333 7.88729372755079E-5 80-81 0.0 5.2581958183671936E-5 5.2581958183671936E-5 2.5774361352681394 1.0516391636734387E-4 82-83 0.0 5.2581958183671936E-5 5.2581958183671936E-5 2.933494865108874 1.0516391636734387E-4 84-85 0.0 5.2581958183671936E-5 5.2581958183671936E-5 3.249328396939099 1.0516391636734387E-4 86-87 0.0 5.2581958183671936E-5 5.2581958183671936E-5 3.5383714210747437 1.0516391636734387E-4 88-89 0.0 5.2581958183671936E-5 5.2581958183671936E-5 3.813900881957185 1.0516391636734387E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9275 0.0 26.504612 1 TACATGG 9315 0.0 25.930136 2 ACATGGG 9215 0.0 24.929697 3 CATGGGA 6550 0.0 23.477924 4 GAGTACT 6810 0.0 21.631634 12-13 AGTACTT 7050 0.0 20.861805 12-13 TACCGAG 160 5.295094E-5 20.622515 7 GTACTTT 7315 0.0 20.009382 14-15 ATGGGAT 2635 0.0 19.871277 5 GTATCAA 18535 0.0 18.430777 1 CATGGGG 2985 0.0 18.17343 4 ATGGGAG 2680 0.0 17.77747 5 GTATAGG 680 0.0 17.347176 1 TACGCGG 115 3.5017776E-5 16.910847 64-65 ACTTTTT 8880 0.0 16.827124 16-17 TACTTTT 8700 0.0 16.715612 14-15 ATCAACG 20500 0.0 16.544682 3 TCAACGC 20575 0.0 16.484371 4 AGTACAT 6965 0.0 16.323511 2 AACGCAG 20850 0.0 16.277586 6 >>END_MODULE