FastQCFastQC Report
Fri 27 May 2016
SRR939575_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939575_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272270
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT138210.6082463791714893No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA128340.5648096396995076No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA101400.4462497854568339No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77900.34282897719021066No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT71020.3125508852381099No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT58720.25841999410281347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51730.22765780475031577No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC51350.22598546827621718No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT47330.20829390873443737No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA44740.19689561539781805No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT42660.18774177364485736No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT41650.1832968793321216No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG41630.18320886162295852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40440.17797180792775508No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT34930.15372292905332552No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG33870.1490579904676821No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC32770.1442170164637125No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC31990.14078432580635222No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30040.13220259916295157No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC24170.10636940152358655No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG24130.10619336610526038No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA23070.10152842751961694No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG22930.10091230355547536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG97700.026.2808382
GTACATG100700.025.8749431
ACATGGG100550.024.4975033
CCGTATA1302.9626652E-421.90129
CATGGGA67400.021.4761944
GAGTACT61750.021.05612212-13
AGTACTT65650.021.03405812-13
CATGGGG35250.021.0030754
GTACCGT1605.0331324E-520.759146
GCTTATA18100.018.87951
GTACTTT69850.018.47933814-15
ATGGGAT27650.017.850325
ATGGGAG30050.016.5826025
ATACTCT18700.016.4960355
GTGTAGC13000.016.4287951
TAGCCCT13950.016.3299334
TATACTC18550.016.1181074
ACTTTTT84500.016.03615616-17
TACTTTT86850.015.84572314-15
GTATAGG6350.015.6957521
Produced by FastQC (version 0.11.5)