FastQCFastQC Report
Fri 27 May 2016
SRR939575_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939575_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272270
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT156370.6881664590915693No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA131230.5775281986735732No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA113550.49972054377340724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86610.3811606895307336No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT80400.3538311908355961No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT69550.3060815836146233No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT65810.2896222720011266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60450.26603352594542024No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC57020.2509384888239514No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG51710.2275697870411527No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA51480.2265575833857772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47820.21045034260893294No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT45300.19936011125438438No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT45290.19931610239980282No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT42840.1885339330273251No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC40410.17783978136401044No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC39470.1737029490333455No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC36380.1601042129676491No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG35990.15838786763896898No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC29820.13123440436215766No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG28690.12626140379444345No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27580.12137642093589229No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27180.11961606675263063No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG23360.10280468430248166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG95550.025.7058472
GTACATG99650.025.0798071
ACATGGG97150.023.9716053
GAGTACT67050.020.84588812-13
CATGGGG36450.020.522694
CATGGGA64200.020.2992234
GTACTTT74300.018.84341814-15
AGTACTT71600.018.4056912-13
ATGGGAG27650.017.3556025
GTATAGG7100.017.2354241
TATAGGG5450.016.4039782
ACTTTTT87450.016.3591116-17
ATGGGAT27700.016.3052025
TATACAG17000.016.0514325
TTTGCGT8650.015.7730833
GCGTGCG6900.015.6606929
AGTACAT73250.015.6095632
ATGGGGA23250.015.5812565
GTGTAGC14400.015.3618081
TACTTTT86650.015.15463414-15
Produced by FastQC (version 0.11.5)