##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939575_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2272270 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.539532273893506 31.0 31.0 34.0 30.0 34.0 2 31.73116046948646 31.0 31.0 34.0 30.0 34.0 3 31.78714721401946 31.0 31.0 34.0 30.0 34.0 4 35.544831380073674 37.0 35.0 37.0 33.0 37.0 5 35.2937687862798 37.0 35.0 37.0 33.0 37.0 6 35.3262504015808 37.0 35.0 37.0 32.0 37.0 7 35.265236965677495 37.0 35.0 37.0 32.0 37.0 8 35.23576907673824 37.0 35.0 37.0 32.0 37.0 9 36.83484004981803 39.0 37.0 39.0 32.0 39.0 10-11 36.71700480136603 39.0 35.0 39.0 32.0 39.0 12-13 36.665106919512205 39.0 35.0 39.0 32.0 39.0 14-15 37.66160447482034 39.0 36.5 41.0 32.0 41.0 16-17 37.641737337552314 39.0 36.0 41.0 32.0 41.0 18-19 37.668478657905965 39.0 36.5 41.0 32.0 41.0 20-21 37.6037783802101 39.0 36.5 41.0 32.0 41.0 22-23 37.468753713247104 39.0 36.0 41.0 32.0 41.0 24-25 37.322534293899935 39.0 36.0 41.0 31.5 41.0 26-27 37.01542840419492 39.0 36.0 40.0 30.5 41.0 28-29 36.82768376997452 39.0 36.0 40.0 30.0 41.0 30-31 36.62696070449374 38.0 35.0 40.0 30.0 41.0 32-33 36.4770308986168 38.0 35.0 40.0 30.0 41.0 34-35 36.46638251616225 38.0 35.0 40.0 30.0 41.0 36-37 36.35425609632658 38.0 35.0 40.0 30.0 41.0 38-39 36.36171075620415 38.0 35.0 40.0 30.0 41.0 40-41 36.28864725582787 38.0 35.0 40.0 30.0 41.0 42-43 36.13515163250846 38.0 35.0 40.0 30.0 41.0 44-45 35.94042103271178 38.0 35.0 40.0 29.0 41.0 46-47 35.645337921989906 38.0 35.0 40.0 28.0 41.0 48-49 35.339509829377675 38.0 34.0 40.0 27.0 41.0 50-51 35.089942876506754 38.0 34.0 40.0 26.5 41.0 52-53 34.825345579530605 38.0 34.0 40.0 26.0 41.0 54-55 34.55598630444446 38.0 33.0 40.0 25.5 41.0 56-57 32.519568977278226 37.0 30.5 39.5 16.0 41.0 58-59 27.468458193788592 32.5 17.5 38.5 6.5 40.0 60-61 28.197867110862706 33.0 19.5 38.0 9.0 40.0 62-63 31.276072165719743 34.5 29.5 38.5 12.5 40.0 64-65 31.473330634123585 35.5 30.5 39.0 5.0 40.0 66-67 24.742965184595143 27.0 18.0 35.5 2.0 38.5 68-69 17.285210164285054 13.0 7.0 28.0 2.0 35.0 70-71 16.121400185717366 12.5 7.0 26.5 2.0 33.5 72-73 17.224475744519797 13.0 7.0 28.5 2.0 34.0 74-75 18.227187790183386 17.0 8.5 29.0 2.0 34.5 76-77 19.436045012256464 21.0 8.0 30.0 2.0 34.0 78-79 22.5009730357748 27.5 10.5 33.5 2.0 35.5 80-81 26.1053450954332 31.5 22.5 34.5 2.0 36.0 82-83 27.41832968793321 33.0 26.0 35.0 2.0 36.0 84-85 27.471058677005814 34.0 27.0 35.0 2.0 36.0 86-87 27.24276010333279 33.0 26.0 35.0 2.0 36.0 88-89 26.773991207030853 33.0 25.5 35.0 2.0 35.0 90-91 25.40550396739824 32.5 16.0 35.0 2.0 35.0 92-93 22.91533796599876 31.5 2.0 34.0 2.0 35.0 94-95 15.638881382934247 4.5 2.0 32.0 2.0 34.5 96-97 13.078019557534976 2.0 2.0 30.0 2.0 34.0 98-99 12.735044690991828 2.0 2.0 29.5 2.0 34.0 100-101 12.436318527287689 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4306612280436859 1101 2 0.4128707233876696 1101 3 0.3911895018248046 1101 4 0.3313204323636967 1101 5 0.5282827650415243 1101 6 0.4476509866466145 1101 7 0.417510923115735 1101 8 0.39570341760267524 1101 9 0.5488665705122315 1101 10-11 0.5203415443891544 1101 12-13 0.5950183857487872 1101 14-15 0.6577641414824171 1101 16-17 0.7287531747844938 1101 18-19 0.5759284303644705 1101 20-21 0.5563872574151105 1101 22-23 0.6742465429713462 1101 24-25 0.583489327533087 1101 26-27 0.629551732698225 1101 28-29 0.7276945802916899 1101 30-31 0.6695018164818407 1101 32-33 0.6440789375028899 1101 34-35 0.4081020956905945 1101 36-37 0.20977487888162472 1101 38-39 0.43374417457368963 1101 40-41 0.4701358664205273 1101 42-43 0.577241359283299 1101 44-45 0.31665938288570317 1101 46-47 0.5290967059311384 1101 48-49 0.3638984926605744 1101 50-51 0.251673789004073 1101 52-53 0.22354278665608973 1101 54-55 0.2083269473907734 1101 56-57 -1.7557040813303786 1101 58-59 -9.700714980430156 1101 60-61 -10.410700986692767 1101 62-63 -4.004445301975473 1101 64-65 -5.1252543806225574 1101 66-67 -9.655998916959817 1101 68-69 -4.562647181269769 1101 70-71 -4.765977792101518 1101 72-73 -4.9179732067858115 1101 74-75 -6.355026746918906 1101 76-77 -7.881946413253749 1101 78-79 -11.05521482319013 1101 80-81 -7.967355696051719 1101 82-83 -4.309614446976159 1101 84-85 -3.4394608113084644 1101 86-87 -3.291340884311179 1101 88-89 -3.3761387126714872 1101 90-91 -9.66912193971366 1101 92-93 -16.68602248661247 1101 94-95 -10.872513721203386 1101 96-97 -9.20453736282598 1101 98-99 -9.155845153093068 1101 100-101 -8.919482073847298 1102 1 0.2939600079195195 1102 2 0.31244489862100977 1102 3 0.34604920905236014 1102 4 0.15012554226090202 1102 5 0.18709389670310372 1102 6 0.26537934772307636 1102 7 0.32335060080796296 1102 8 0.3690757490291219 1102 9 0.4319267738184607 1102 10-11 0.3392455832725503 1102 12-13 0.30852362611754813 1102 14-15 0.3940079942646122 1102 16-17 0.3898468418956256 1102 18-19 0.4218049555196757 1102 20-21 0.49036936417238763 1102 22-23 0.5096867769599029 1102 24-25 0.47434986633762577 1102 26-27 0.39107552583627836 1102 28-29 0.4092773006954431 1102 30-31 0.4556632284422122 1102 32-33 0.4648088613705781 1102 34-35 0.2488522354815501 1102 36-37 0.32518844505651856 1102 38-39 0.3157950154700728 1102 40-41 0.28595590169086194 1102 42-43 0.4408268898437555 1102 44-45 0.5333102782647146 1102 46-47 0.5627820257298453 1102 48-49 0.41105105836232525 1102 50-51 0.4280440988381926 1102 52-53 0.23643796494332747 1102 54-55 0.09644113151338729 1102 56-57 -3.1526372827583984 1102 58-59 -10.925749748950665 1102 60-61 -11.00148625344259 1102 62-63 -4.668384252754716 1102 64-65 -5.803971744810266 1102 66-67 -7.278754017537619 1102 68-69 -4.481755530015953 1102 70-71 -4.502637433376412 1102 72-73 -4.76840476656616 1102 74-75 -5.493862825890044 1102 76-77 -7.682934269362256 1102 78-79 -10.223870822731328 1102 80-81 -6.152989486752393 1102 82-83 -3.7856499753859794 1102 84-85 -3.1927878361267368 1102 86-87 -3.1026058849563647 1102 88-89 -3.288200163343202 1102 90-91 -9.459803976217021 1102 92-93 -13.236748682721261 1102 94-95 -10.481037385303633 1102 96-97 -9.227833705320997 1102 98-99 -9.37665393339903 1102 100-101 -9.19666890625586 1103 1 0.24324941486134577 1103 2 0.27974529364718137 1103 3 0.284457558439712 1103 4 0.25019494751624194 1103 5 0.3382864346651857 1103 6 0.27533673159900474 1103 7 0.25509864027166174 1103 8 0.2868934402341097 1103 9 0.4209036625170839 1103 10-11 0.4629536401199914 1103 12-13 0.3593396243532254 1103 14-15 0.5398686518631877 1103 16-17 0.5341987459996105 1103 18-19 0.49793765577329197 1103 20-21 0.37978117084956153 1103 22-23 0.5621386635504209 1103 24-25 0.5163144242520943 1103 26-27 0.5081265159963024 1103 28-29 0.4717092738154989 1103 30-31 0.5570169552449684 1103 32-33 0.5019993755532539 1103 34-35 0.4694292720717428 1103 36-37 0.42423720387001396 1103 38-39 0.3631929670420675 1103 40-41 0.32256101173386753 1103 42-43 0.42290800966836883 1103 44-45 0.42094219689840173 1103 46-47 0.40661468771327236 1103 48-49 0.3918248296622835 1103 50-51 0.41511331598582757 1103 52-53 0.537802718749262 1103 54-55 0.37563836568153164 1103 56-57 -2.47811809252962 1103 58-59 -10.10545292797082 1103 60-61 -10.38620217838113 1103 62-63 -4.317960037455723 1103 64-65 -5.5281734222120456 1103 66-67 -6.516880854020137 1103 68-69 -4.025930029098117 1103 70-71 -3.9768848404090438 1103 72-73 -4.543318658644232 1103 74-75 -4.945353092938117 1103 76-77 -6.408566213941835 1103 78-79 -9.085147555105962 1103 80-81 -4.905700507917754 1103 82-83 -2.7535954500984694 1103 84-85 -2.2226950147058098 1103 86-87 -2.103837017429594 1103 88-89 -2.1261988919946013 1103 90-91 -5.213823967525151 1103 92-93 -10.27711594679204 1103 94-95 -8.45746813896687 1103 96-97 -7.9489967946981 1103 98-99 -8.253933441983877 1103 100-101 -8.2537294315304 1104 1 0.22581968088689308 1104 2 0.1430972859256343 1104 3 0.20338898571709763 1104 4 0.16986327853735617 1104 5 0.1680206289776507 1104 6 0.15332914343255766 1104 7 0.2255424241532964 1104 8 0.21602187841591558 1104 9 0.19195378882773184 1104 10-11 0.2676037922277743 1104 12-13 0.3217655950251128 1104 14-15 0.37463374409891514 1104 16-17 0.28883902264448835 1104 18-19 0.33651240478263134 1104 20-21 0.35209194855234927 1104 22-23 0.44480167464684683 1104 24-25 0.4429444985727429 1104 26-27 0.3694370906932747 1104 28-29 0.4422347730302221 1104 30-31 0.42481913524881776 1104 32-33 0.4246645156455813 1104 34-35 0.5234451226477432 1104 36-37 0.4949305859540587 1104 38-39 0.39038979033101384 1104 40-41 0.24257415947446503 1104 42-43 0.33179678740486196 1104 44-45 0.2991916639165595 1104 46-47 0.34624015666035035 1104 48-49 0.22326570183971484 1104 50-51 0.15375680847717632 1104 52-53 0.3408148998304412 1104 54-55 0.29452847724677866 1104 56-57 -2.804416120441818 1104 58-59 -10.33474880929817 1104 60-61 -10.39522686740482 1104 62-63 -3.9225159970685617 1104 64-65 -5.330201263322259 1104 66-67 -6.944089439919807 1104 68-69 -4.202568623066718 1104 70-71 -4.100530766038565 1104 72-73 -4.353335089655605 1104 74-75 -5.07381802460138 1104 76-77 -6.654935561384438 1104 78-79 -9.0718897919661 1104 80-81 -4.909067234606713 1104 82-83 -2.662525085501507 1104 84-85 -2.259910485946776 1104 86-87 -2.075974287463225 1104 88-89 -1.941121266092523 1104 90-91 -4.645652767672896 1104 92-93 -6.867381983334553 1104 94-95 -7.89722772366243 1104 96-97 -7.630598129406303 1104 98-99 -8.168499511578958 1104 100-101 -8.317097748553646 1105 1 0.36134371601091786 1105 2 0.32852247418964 1105 3 0.3159048914757534 1105 4 0.22514340334711136 1105 5 0.31477922982859496 1105 6 0.13124453873927422 1105 7 0.21619967138198604 1105 8 0.1966454722147546 1105 9 0.27721599351632165 1105 10-11 0.389013713898791 1105 12-13 0.3400416235975072 1105 14-15 0.3499963293515833 1105 16-17 0.4032549969428345 1105 18-19 0.40615669551421263 1105 20-21 0.366956402869242 1105 22-23 0.3417815251510632 1105 24-25 0.4393252505133489 1105 26-27 0.5173287245944493 1105 28-29 0.6316354836708982 1105 30-31 0.5764411924082822 1105 32-33 0.5002575968056746 1105 34-35 0.3993016853601006 1105 36-37 0.24187069958023955 1105 38-39 0.303253934971778 1105 40-41 0.23397624384433158 1105 42-43 0.2976848974558308 1105 44-45 0.2963352318258856 1105 46-47 0.4313062453627481 1105 48-49 0.23094376194623578 1105 50-51 0.3694662309972401 1105 52-53 0.5400536982870534 1105 54-55 0.45631279149026227 1105 56-57 1.4802868693271236 1105 58-59 -8.854866566949447 1105 60-61 -10.181181770634698 1105 62-63 -4.025136392837592 1105 64-65 -5.436104813150028 1105 66-67 -6.8540784398126675 1105 68-69 -4.075691853619865 1105 70-71 -4.1449344340129155 1105 72-73 -2.607354274899169 1105 74-75 -4.768437732038155 1105 76-77 -6.741843379165719 1105 78-79 -9.482240202984112 1105 80-81 -5.0785516238475985 1105 82-83 -2.51988675493471 1105 84-85 -2.1084939011021184 1105 86-87 -2.0933304605120107 1105 88-89 -2.0586754630847004 1105 90-91 -4.599925821299703 1105 92-93 -6.956397176544574 1105 94-95 -8.2184677270674 1105 96-97 -8.125826768724606 1105 98-99 -8.29475857987942 1105 100-101 -8.220728114132925 1106 1 0.18027073678222294 1106 2 0.15668068880014374 1106 3 0.08214282888583924 1106 4 0.15296122353646524 1106 5 0.039223799108754065 1106 6 0.2112286791761946 1106 7 0.207150032089352 1106 8 0.16925353177116165 1106 9 0.16916295027947115 1106 10-11 0.17476001299485233 1106 12-13 0.16070704950574566 1106 14-15 0.2260652012139559 1106 16-17 0.18580088831459562 1106 18-19 0.3400390888753435 1106 20-21 0.2327084172698548 1106 22-23 0.23027792784719026 1106 24-25 0.4066881747953346 1106 26-27 0.2969335137265787 1106 28-29 0.3444408189321351 1106 30-31 0.2916309576167322 1106 32-33 0.24702197726910669 1106 34-35 0.4529074970595346 1106 36-37 0.43059034109550254 1106 38-39 0.2831176632228889 1106 40-41 0.3174080888374533 1106 42-43 0.19133301217876664 1106 44-45 0.34500996505061465 1106 46-47 0.42326818241135555 1106 48-49 0.4658246646061315 1106 50-51 0.4179647016240722 1106 52-53 0.5290185605620508 1106 54-55 0.3772820159592598 1106 56-57 1.5844918517375035 1106 58-59 -8.670601728528922 1106 60-61 -10.308666442301849 1106 62-63 -4.178168353765098 1106 64-65 -5.8194102212773196 1106 66-67 -7.2268821793478395 1106 68-69 -4.185890374967993 1106 70-71 -3.999882731674445 1106 72-73 -4.6592489553509555 1106 74-75 -5.39329596887484 1106 76-77 -6.747817989210162 1106 78-79 -9.714720596314304 1106 80-81 -5.175818047410285 1106 82-83 -2.5380200241133437 1106 84-85 -2.0788272368778884 1106 86-87 -1.9023273431471708 1106 88-89 -1.833320678633914 1106 90-91 -4.2553293893757385 1106 92-93 -6.602043816035048 1106 94-95 -7.837832921188291 1106 96-97 -7.380227024040774 1106 98-99 -6.698279435449116 1106 100-101 -7.330446696444302 1107 1 0.10835143462438168 1107 2 -0.029702948004448615 1107 3 -0.025580018547017858 1107 4 0.02595724234632968 1107 5 -0.09248422280038682 1107 6 -0.06595262875815422 1107 7 0.06854382659820857 1107 8 0.08729148851351454 1107 9 0.19735845380238004 1107 10-11 0.09862231074629335 1107 12-13 0.04627992040369122 1107 14-15 0.035939749629200435 1107 16-17 -0.01895008257294961 1107 18-19 0.02753200210492679 1107 20-21 0.020472635693572272 1107 22-23 -0.06264349089275356 1107 24-25 0.13396090206806122 1107 26-27 0.08297210355375029 1107 28-29 0.021052389895906742 1107 30-31 0.07493681002922159 1107 32-33 -0.0615253765308168 1107 34-35 -0.05000260647030785 1107 36-37 -0.10797842262527269 1107 38-39 -0.10924079034931822 1107 40-41 -0.19545459632411166 1107 42-43 -0.18080924765059336 1107 44-45 -0.06109898853261342 1107 46-47 0.019186242022115607 1107 48-49 -0.13464093621026763 1107 50-51 -0.06597884201310933 1107 52-53 -0.22853514928351615 1107 54-55 -0.3321670900052851 1107 56-57 0.669876411397091 1107 58-59 -9.136044864283093 1107 60-61 -10.807886700701246 1107 62-63 -4.519019963091921 1107 64-65 -5.9759181994059745 1107 66-67 -7.223109261519731 1107 68-69 -4.336613186179578 1107 70-71 -4.338227789675722 1107 72-73 -2.7331982235237966 1107 74-75 -4.862982505270917 1107 76-77 -6.636144857834038 1107 78-79 -7.833686014449505 1107 80-81 -3.1023349312470927 1107 82-83 -2.0703313664106524 1107 84-85 -1.8103860822697584 1107 86-87 -1.7945363839764177 1107 88-89 -1.5444316322612366 1107 90-91 -1.1433056597187061 1107 92-93 -4.000303383962528 1107 94-95 -6.547356044532741 1107 96-97 -6.6489622796212124 1107 98-99 -6.518073100191332 1107 100-101 -7.191829467088064 1108 1 -0.11394013248774115 1108 2 -0.09730529007661204 1108 3 -0.22630395173424844 1108 4 0.03021611711907468 1108 5 -0.16484664528515935 1108 6 -0.27418709412965114 1108 7 -0.2746914077463245 1108 8 -0.18009073502694406 1108 9 -0.1278546615820204 1108 10-11 -0.13145819798429415 1108 12-13 -0.053470643967592935 1108 14-15 -0.1258714015783653 1108 16-17 -0.16839518613713977 1108 18-19 -0.10792019523552199 1108 20-21 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2316 32-33 -0.6937090665191832 2316 34-35 -0.5339717497674954 2316 36-37 -0.5378619125045887 2316 38-39 -0.5704756346183757 2316 40-41 -0.3749291554939944 2316 42-43 -0.6526404562305501 2316 44-45 -0.7270201646791605 2316 46-47 -0.684219195210531 2316 48-49 -0.5431437439200835 2316 50-51 -0.5895868615518438 2316 52-53 -0.6844999166005437 2316 54-55 -0.35704581605261865 2316 56-57 1.3097384429536092 2316 58-59 6.3404279186379355 2316 60-61 5.402611787238808 2316 62-63 1.775622341984441 2316 64-65 1.0363093206470886 2316 66-67 7.529530519709368 2316 68-69 14.561647031467118 2316 70-71 15.336402135058544 2316 72-73 13.943063559582725 2316 74-75 12.278924531147805 2316 76-77 10.357326654731796 2316 78-79 7.831838527014099 2316 80-81 3.807867538638181 2316 82-83 2.056929697202662 2316 84-85 1.631959254795948 2316 86-87 1.4209700998940065 2316 88-89 1.6240738173377025 2316 90-91 2.715140375480253 2316 92-93 4.909359466017516 2316 94-95 11.726633151827073 2316 96-97 13.41143053275407 2316 98-99 13.131157800917698 2316 100-101 11.973730557194832 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 23.0 5 82.0 6 200.0 7 453.0 8 1018.0 9 1850.0 10 3141.0 11 4992.0 12 7529.0 13 10623.0 14 13399.0 15 18261.0 16 25339.0 17 34149.0 18 38403.0 19 39246.0 20 40732.0 21 40938.0 22 40950.0 23 34861.0 24 35151.0 25 41077.0 26 57154.0 27 84222.0 28 120864.0 29 163272.0 30 202571.0 31 219846.0 32 219033.0 33 199203.0 34 176569.0 35 157525.0 36 127439.0 37 80513.0 38 29646.0 39 1980.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.610092920332555 19.434827450961404 14.310849303900858 22.644230324805182 2 14.915074309978769 22.288680447725984 39.26937323291539 23.52687200937985 3 20.388185015229286 26.16087810103302 30.295647474526543 23.155289409211154 4 12.324195628160387 17.00400920665238 39.221439353597944 31.450355811589294 5 12.62683355832063 39.229719301353846 34.68744394599071 13.456003194334814 6 29.729961668287658 37.89818111404015 17.940429614438425 14.431427603233772 7 26.791886527569346 33.834887579380975 21.576397171110827 17.796828721938855 8 24.91013273245784 34.381535401190014 20.533042284265747 20.1752895820864 9 25.659758743459182 17.66942308792529 21.03218367535548 35.63863449326005 10-11 24.259573026092852 26.353448313800737 28.369647973172203 21.01733068693421 12-13 26.9212285511845 23.840982805740516 27.858375105071143 21.379413538003845 14-15 23.177775044322683 25.275227510626554 27.57276742304401 23.974230022006758 16-17 21.2851025626356 28.4757533215683 28.08301830328262 22.156125812513476 18-19 21.10604568303183 28.688190555155074 29.202610961920833 21.003152799892266 20-21 22.718008863383314 26.944377208694387 28.21429671649936 22.12331721142294 22-23 21.775543098563656 26.933683938365395 28.23949697713182 23.051275985939125 24-25 22.486610305993565 26.879046064068092 28.735141510471905 21.899202119466437 26-27 22.638835680809024 26.09094411805673 29.58204451200312 21.68817568913112 28-29 21.63077095731794 27.428634928808222 29.440026934159842 21.500567179714 30-31 21.839602056443706 27.330318195952824 28.77251807398875 22.057561673614718 32-33 21.83412224973093 27.220025266156135 28.694229119277953 22.251623364834977 34-35 22.707569930823958 26.58167904394646 28.550938440453944 22.159812584775636 36-37 22.773680050682387 26.222166319649737 29.440700636437573 21.563452993230307 38-39 22.68150854818714 26.416334557796635 29.50988132192694 21.392275572089286 40-41 22.08449325285937 26.95086787976845 29.437656032859323 21.526982834512857 42-43 21.441286666228976 27.52691900790176 28.521039721206453 22.510754604662804 44-45 22.06457218591601 27.202165683226532 28.10420323724524 22.62905889361222 46-47 22.484360099579007 27.25930171774008 27.636170503827394 22.620167678853516 48-49 22.503107725906833 26.95902383812465 28.104217805821047 22.433650630147465 50-51 22.264883949820995 27.406562005186373 28.640859038309202 21.687695006683423 52-53 22.2252247966545 27.789244822431126 28.036329535553854 21.949200845360515 54-55 21.57332007963669 26.978590978487038 28.571985387755404 22.876103554120863 56-57 21.573111620644557 27.550605661603463 28.500401473315247 22.375881244436734 58-59 21.32213273637353 26.60573223589659 29.83761772262427 22.234517305105612 60-61 22.043523161119126 26.780189832946743 29.122301865483717 22.053985140450415 62-63 22.786376959571868 26.761854254504485 28.31005906222218 22.141709723701467 64-65 21.91819631826153 27.582217855286 28.63886710316446 21.86071872328801 66-67 21.54206138829125 26.621912547802257 29.289733204477596 22.546292859428902 68-69 21.75528607493434 26.765043327183374 29.500992714056444 21.97867788382584 70-71 21.79359170955939 26.563781463464093 29.25869064193342 22.383936185043098 72-73 21.091947945363962 26.22071971915515 29.848600295017246 22.83873204046364 74-75 21.357799005548827 27.36694062795106 28.9280394726975 22.347220893802618 76-77 20.05555302622499 26.944222012139 29.703372014236855 23.29685294739915 78-79 20.705770046151216 27.00651412044271 29.39811961381254 22.889596219593532 80-81 21.89281423560314 27.66095818557968 27.90425497485904 22.541972603958136 82-83 21.98708733640501 27.366564338542304 27.819636393722476 22.826711931330205 84-85 21.933110369364847 27.229564502915704 27.54154389088775 23.2957812368317 86-87 22.123928077820267 27.40221581010812 27.67559983743068 22.798256274640924 88-89 22.470439769965463 26.84133800267794 27.448132757256793 23.2400894700998 90-91 21.361928076101673 26.883825659300932 28.42863174129384 23.325614523303557 92-93 20.458438164745278 27.832944196186972 28.842484498940824 22.866133140126923 94-95 19.654041063427226 27.59945746899542 29.205451623832296 23.541049843745053 96-97 19.337902724993608 27.41186264946245 28.97229727383383 24.277937351710115 98-99 19.895892340842103 27.59689953624283 28.33637103430482 24.17083708861025 100-101 20.580796329914136 27.26718847905069 28.21725010545011 23.934765085585063 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 595.0 1 443.0 2 297.0 3 336.5 4 363.5 5 426.0 6 595.0 7 846.0 8 1120.5 9 1348.0 10 1521.0 11 1670.5 12 1847.5 13 2084.5 14 2326.0 15 2570.5 16 2829.5 17 3116.5 18 3324.0 19 3639.5 20 4124.5 21 4715.0 22 5634.5 23 6727.0 24 8126.5 25 10154.0 26 12724.5 27 15663.0 28 18719.5 29 22409.5 30 26422.5 31 30775.5 32 36129.0 33 41813.0 34 47158.0 35 52000.0 36 57297.0 37 61873.5 38 65011.5 39 67598.5 40 70347.0 41 72974.5 42 75504.5 43 79240.0 44 84458.0 45 91407.0 46 100332.0 47 110765.0 48 119466.5 49 113984.0 50 98351.0 51 86434.0 52 77235.5 53 70415.0 54 64542.0 55 58373.5 56 52282.0 57 45879.0 58 39576.0 59 33404.5 60 27083.0 61 21003.0 62 15721.5 63 11403.0 64 8106.5 65 5716.5 66 3983.5 67 2773.0 68 1893.0 69 1311.0 70 887.0 71 556.5 72 339.0 73 200.5 74 108.0 75 57.5 76 30.0 77 14.5 78 11.5 79 6.5 80 5.5 81 5.0 82 2.0 83 1.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023720772619451033 2 0.0073054698605359404 3 1.3202656374462543E-4 4 0.0 5 0.1431608039537555 6 0.0 7 0.0 8 0.0013202656374462541 9 0.0 10-11 0.0 12-13 0.0 14-15 5.941195368508144E-4 16-17 0.0 18-19 1.7603541832616722E-4 20-21 0.0 22-23 3.3006640936156353E-4 24-25 0.0 26-27 2.8605755478002177E-4 28-29 0.002750553411346363 30-31 0.012740563401356353 32-33 0.002662535702183279 34-35 0.004224850039828014 36-37 0.10773367601561434 38-39 0.10702953434230968 40-41 0.11952804904346755 42-43 0.12221258917294159 44-45 0.1182077834060213 46-47 0.11066026484528688 48-49 0.11105634453652076 50-51 0.10896592394389751 52-53 0.08784167374475745 54-55 0.07518912805256417 56-57 0.5487244033499541 58-59 1.5668692540939235 60-61 0.5150576295950745 62-63 0.003960796912338763 64-65 0.11838381882434747 66-67 1.8881338925391788 68-69 2.857824994388871 70-71 2.767364793796512 72-73 2.619319006984205 74-75 1.9941732276534039 76-77 1.403354354896205 78-79 0.9278606855699367 80-81 0.05173240856060239 82-83 1.9803984561693813E-4 84-85 1.9803984561693813E-4 86-87 3.7407526394310535E-4 88-89 1.1002213645385451E-4 90-91 0.24262081530804 92-93 0.38353716767813684 94-95 1.9936231169711345 96-97 2.416394178508716 98-99 1.4122001346670952 100-101 0.9332297658288847 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2272270.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.45271105035244 #Duplication Level Percentage of deduplicated Percentage of total 1 72.59722356021365 35.90129519781084 2 14.692594726834848 14.531772832121915 3 5.3599469584796315 7.951917245487258 4 2.496163061468841 4.937681224535269 5 1.394759254697103 3.448731320367038 6 0.8256681751889584 2.44989178146548 7 0.5427187536265623 1.878723959329126 8 0.36621054517636903 1.448808341935921 9 0.27535098109418277 1.225516725493351 >10 1.3230739406580312 11.400068168173103 >50 0.07979300219286523 2.7274411074749896 >100 0.040062490738348326 3.621411462012673 >500 0.002144849876898842 0.6800325759490338 >1k 0.003306643560218964 3.7122698565015795 >5k 7.149499589662625E-4 2.3115450588068875 >10k+ 2.681062346123484E-4 1.7728931425355772 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15637 0.6881664590915693 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 13123 0.5775281986735732 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 11355 0.49972054377340724 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8661 0.3811606895307336 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8040 0.3538311908355961 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6955 0.3060815836146233 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6581 0.2896222720011266 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6045 0.26603352594542024 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5702 0.2509384888239514 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5171 0.2275697870411527 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 5148 0.2265575833857772 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4782 0.21045034260893294 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4530 0.19936011125438438 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4529 0.19931610239980282 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4284 0.1885339330273251 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4041 0.17783978136401044 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3947 0.1737029490333455 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3638 0.1601042129676491 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3599 0.15838786763896898 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2982 0.13123440436215766 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2869 0.12626140379444345 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2758 0.12137642093589229 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2718 0.11961606675263063 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2336 0.10280468430248166 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 4.4008854581541806E-5 0.0 0.0 0.0 14-15 0.0 6.60132818723127E-5 0.0 4.4008854581541806E-5 0.0 16-17 0.0 8.801770916308361E-5 0.0 2.2004427290770904E-4 0.0 18-19 0.0 8.801770916308361E-5 0.0 2.8605755478002177E-4 0.0 20-21 0.0 1.3202656374462543E-4 0.0 6.161239641415853E-4 0.0 22-23 0.0 1.3202656374462543E-4 0.0 0.001584318764935505 0.0 24-25 0.0 1.3202656374462543E-4 0.0 0.002706544556764821 0.0 26-27 0.0 1.3202656374462543E-4 0.0 0.0031026242479986975 0.0 28-29 0.0 1.3202656374462543E-4 0.0 0.005633133386437351 0.0 30-31 0.0 1.540309910353963E-4 0.0 0.011596333182236265 0.0 32-33 0.0 1.7603541832616722E-4 0.0 0.02501903382960652 0.0 34-35 0.0 1.7603541832616722E-4 0.0 0.04671539913830663 0.0 36-37 0.0 1.7603541832616722E-4 0.0 0.07694948223582584 0.0 38-39 0.0 1.7603541832616722E-4 0.0 0.12159646520880002 0.0 40-41 0.0 1.7603541832616722E-4 0.0 0.17357092246960087 0.0 42-43 0.0 1.7603541832616722E-4 0.0 0.22631553468557875 0.0 44-45 0.0 1.7603541832616722E-4 0.0 0.28594753264356787 0.0 46-47 0.0 1.7603541832616722E-4 0.0 0.35827608514833187 0.0 48-49 0.0 1.7603541832616722E-4 0.0 0.4242673625933538 0.0 50-51 0.0 1.7603541832616722E-4 0.0 0.49061071087502806 0.0 52-53 0.0 1.7603541832616722E-4 0.0 0.5536093862085052 0.0 54-55 0.0 1.7603541832616722E-4 0.0 0.6246616819304045 0.0 56-57 0.0 1.9803984561693813E-4 0.0 0.6853278879710598 2.2004427290770903E-5 58-59 4.4008854581541806E-5 2.2004427290770904E-4 0.0 0.7272023131053968 4.4008854581541806E-5 60-61 4.4008854581541806E-5 2.2004427290770904E-4 0.0 0.765027923618232 4.4008854581541806E-5 62-63 1.1002213645385452E-4 2.2004427290770904E-4 0.0 0.798254608827296 4.4008854581541806E-5 64-65 1.3202656374462543E-4 2.2004427290770904E-4 0.0 0.8296549265712261 4.4008854581541806E-5 66-67 1.3202656374462543E-4 2.2004427290770904E-4 0.0 0.8554001065014281 4.4008854581541806E-5 68-69 1.540309910353963E-4 2.2004427290770904E-4 0.0 0.8918834469495263 8.801770916308361E-5 70-71 1.7603541832616722E-4 2.2004427290770904E-4 0.0 0.9519555334533308 8.801770916308361E-5 72-73 1.7603541832616722E-4 2.2004427290770904E-4 4.4008854581541806E-5 1.037882822023791 1.1002213645385452E-4 74-75 1.7603541832616722E-4 2.2004427290770904E-4 4.4008854581541806E-5 1.146430661849164 1.3202656374462543E-4 76-77 1.7603541832616722E-4 2.2004427290770904E-4 4.4008854581541806E-5 1.3068649412261748 1.3202656374462543E-4 78-79 1.7603541832616722E-4 2.2004427290770904E-4 4.4008854581541806E-5 1.5652409264744067 1.3202656374462543E-4 80-81 1.7603541832616722E-4 2.2004427290770904E-4 4.4008854581541806E-5 1.8869456534654772 1.3202656374462543E-4 82-83 1.7603541832616722E-4 2.2004427290770904E-4 4.4008854581541806E-5 2.201807003569118 1.7603541832616722E-4 84-85 1.7603541832616722E-4 2.2004427290770904E-4 4.4008854581541806E-5 2.4627795112376614 1.7603541832616722E-4 86-87 1.7603541832616722E-4 2.2004427290770904E-4 4.4008854581541806E-5 2.6619195782191376 1.7603541832616722E-4 88-89 1.7603541832616722E-4 2.2004427290770904E-4 4.4008854581541806E-5 2.838791164782354 1.7603541832616722E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 9555 0.0 25.705847 2 GTACATG 9965 0.0 25.079807 1 ACATGGG 9715 0.0 23.971605 3 GAGTACT 6705 0.0 20.845888 12-13 CATGGGG 3645 0.0 20.52269 4 CATGGGA 6420 0.0 20.299223 4 GTACTTT 7430 0.0 18.843418 14-15 AGTACTT 7160 0.0 18.40569 12-13 ATGGGAG 2765 0.0 17.355602 5 GTATAGG 710 0.0 17.235424 1 TATAGGG 545 0.0 16.403978 2 ACTTTTT 8745 0.0 16.35911 16-17 ATGGGAT 2770 0.0 16.305202 5 TATACAG 1700 0.0 16.051432 5 TTTGCGT 865 0.0 15.773083 3 GCGTGCG 690 0.0 15.660692 9 AGTACAT 7325 0.0 15.609563 2 ATGGGGA 2325 0.0 15.581256 5 GTGTAGC 1440 0.0 15.361808 1 TACTTTT 8665 0.0 15.154634 14-15 >>END_MODULE