FastQCFastQC Report
Fri 27 May 2016
SRR939574_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939574_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212137
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55160.2493516450382594No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA52420.23696543206862866No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA41470.18746578534692923No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31620.1429387058758115No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31220.1411304996028727No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25660.11599643240902349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG86950.030.631881
TACATGG95800.024.807422
ACATGGG96000.023.8698443
GTGTAGG7500.021.8538841
GTATAAT14350.021.4160441
GAGTACT60350.021.15756812-13
AGTACTT66150.020.51557512-13
CATGGGA64000.020.3731884
GCTTATA14200.020.199451
CATGGGG36100.019.6297134
GTACTTT66550.019.32919714-15
GTCTTAG8801.8189894E-1218.6254671
CTTTGCG6700.018.3348622
GTACTAT6305.664333E-817.8863621
TTTGCGT6100.017.8146423
TACTTTT82400.016.55640414-15
GTATCGA2002.8763403E-416.5366342
ATGGGAG27350.016.2217275
ACCGGTC1750.002264256616.1830148
ACTTTTT83550.016.0483816-17
Produced by FastQC (version 0.11.5)