##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939574_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2212137 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.46065998624859 31.0 31.0 34.0 30.0 34.0 2 31.646014238720298 31.0 31.0 34.0 30.0 34.0 3 31.712134013399712 31.0 31.0 34.0 30.0 34.0 4 35.48815828314431 37.0 35.0 37.0 33.0 37.0 5 35.25695063190028 37.0 35.0 37.0 33.0 37.0 6 35.27110255829544 37.0 35.0 37.0 32.0 37.0 7 35.18745131969675 37.0 35.0 37.0 32.0 37.0 8 35.15817646013787 37.0 35.0 37.0 32.0 37.0 9 36.74192692405579 39.0 35.0 39.0 32.0 39.0 10-11 36.613856420285 39.0 35.0 39.0 32.0 39.0 12-13 36.557996181972456 38.0 35.0 39.0 32.0 39.0 14-15 37.566811865630385 39.0 36.0 41.0 32.0 41.0 16-17 37.536530061203266 39.0 36.0 41.0 32.0 41.0 18-19 37.5502624837431 39.0 36.0 41.0 32.0 41.0 20-21 37.48205129248324 39.0 36.0 41.0 32.0 41.0 22-23 37.35070499702324 39.0 36.0 41.0 31.5 41.0 24-25 37.19555004956746 39.0 36.0 40.5 31.0 41.0 26-27 36.86278426697804 39.0 36.0 40.0 30.5 41.0 28-29 36.67563130131633 38.5 35.5 40.0 30.0 41.0 30-31 36.465870332624064 38.0 35.0 40.0 30.0 41.0 32-33 36.31763335634275 38.0 35.0 40.0 30.0 41.0 34-35 36.29673523836905 38.0 35.0 40.0 30.0 41.0 36-37 36.20078096428928 38.0 35.0 40.0 30.0 41.0 38-39 36.22928937041422 38.0 35.0 40.0 30.0 41.0 40-41 36.144025211820065 38.0 35.0 40.0 30.0 41.0 42-43 35.99389255728737 38.0 35.0 40.0 29.0 41.0 44-45 35.793795094969255 38.0 35.0 40.0 28.5 41.0 46-47 35.503438304228 38.0 34.0 40.0 27.5 41.0 48-49 35.20206411266572 38.0 34.0 40.0 27.0 41.0 50-51 34.96061500711755 38.0 34.0 40.0 26.0 41.0 52-53 34.70784246183668 38.0 33.0 40.0 26.0 41.0 54-55 34.44702882326004 38.0 33.0 40.0 25.0 41.0 56-57 33.72004401174068 37.5 32.5 40.0 22.0 41.0 58-59 30.68294911210291 35.5 27.0 39.0 8.5 40.5 60-61 30.385539412794053 34.5 26.0 39.0 10.5 40.0 62-63 32.09851062569814 35.5 30.5 39.0 18.5 40.0 64-65 32.10133798223166 36.0 31.0 39.0 16.0 40.0 66-67 27.26258342046627 32.0 18.5 37.0 4.5 39.5 68-69 19.7318057154688 20.0 8.0 32.0 2.0 37.0 70-71 17.7554220195223 15.5 7.5 29.0 2.0 35.0 72-73 19.464848696079855 20.0 7.5 31.0 2.0 35.5 74-75 20.929839788403704 23.5 9.0 32.5 2.0 36.0 76-77 22.79341717985821 27.5 11.5 33.0 2.0 35.5 78-79 24.99694390537295 30.5 13.0 34.5 2.0 36.0 80-81 26.588898201151196 32.0 22.5 35.0 2.0 36.0 82-83 27.625782444758165 33.0 26.5 35.0 2.0 36.0 84-85 27.642013130289854 33.0 27.0 35.0 2.0 36.0 86-87 27.367470007508576 33.0 26.0 35.0 2.0 36.0 88-89 26.88748300851168 33.0 25.5 35.0 2.0 35.0 90-91 25.100149538658773 32.0 16.0 34.5 2.0 35.0 92-93 23.598204812812227 31.0 2.0 34.0 2.0 35.0 94-95 19.827430443955322 27.5 2.0 34.0 2.0 35.0 96-97 17.119439935230048 18.5 2.0 33.0 2.0 35.0 98-99 17.377947658757122 21.5 2.0 33.0 2.0 35.0 100-101 16.573622474557407 10.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.29149525460288217 1101 2 0.23332999258342113 1101 3 0.19281047020852782 1101 4 0.30607986907600804 1101 5 0.3300108163211988 1101 6 0.31317396409626497 1101 7 0.278987224014827 1101 8 0.38346179109095857 1101 9 0.30295497705836993 1101 10-11 0.3531547784234448 1101 12-13 0.266249654753139 1101 14-15 0.488688335273018 1101 16-17 0.4812873429321911 1101 18-19 0.3726993628058821 1101 20-21 0.45342785095645155 1101 22-23 0.49810616951489806 1101 24-25 0.37066450159027653 1101 26-27 0.4398383057490918 1101 28-29 0.4558428712941591 1101 30-31 0.22019166838505555 1101 32-33 0.3550635823091355 1101 34-35 0.11899833385196956 1101 36-37 0.10358142745018739 1101 38-39 0.10163268751784926 1101 40-41 0.13652044927504647 1101 42-43 0.32714561060293335 1101 44-45 0.1564806641884573 1101 46-47 0.12201303729205648 1101 48-49 0.034163173518635404 1101 50-51 0.1887051313159347 1101 52-53 0.13151591323418899 1101 54-55 -0.005099364076421864 1101 56-57 -3.544012360251955 1101 58-59 -10.528778725968262 1101 60-61 -11.97797737557985 1101 62-63 -4.307818087669158 1101 64-65 -1.4373570682377483 1101 66-67 -2.2338653058683526 1101 68-69 -4.842408274578098 1101 70-71 -4.677212728067554 1101 72-73 -3.6077528062123 1101 74-75 -6.108762055421304 1101 76-77 -5.040070859037371 1101 78-79 1.0187501085943502 1101 80-81 1.773164911391973 1101 82-83 0.4603951417121124 1101 84-85 0.17883545943493218 1101 86-87 0.22369593923699682 1101 88-89 -0.09014253167653408 1101 90-91 1.2716113576389922 1101 92-93 0.6986376642431225 1101 94-95 -8.81259152606456 1101 96-97 -9.539288474587021 1101 98-99 -8.627668173305096 1101 100-101 -9.590975679258655 1102 1 0.1959775465095248 1102 2 0.22035000150318496 1102 3 0.25587533933610374 1102 4 0.29038758319463653 1102 5 0.313653055536264 1102 6 0.22626737203662373 1102 7 0.22446166239462428 1102 8 0.32754971573962877 1102 9 0.304968015566935 1102 10-11 0.29517788489352625 1102 12-13 0.33393832842565985 1102 14-15 0.35560221525199154 1102 16-17 0.3537656453121727 1102 18-19 0.3231384654835807 1102 20-21 0.30356862520374506 1102 22-23 0.34230834171359703 1102 24-25 0.2175078061559006 1102 26-27 0.045045365357921696 1102 28-29 0.17335643553712998 1102 30-31 0.32084479369887475 1102 32-33 0.18753733620045665 1102 34-35 0.19292345804933575 1102 36-37 0.052738406426719564 1102 38-39 0.14580573019093634 1102 40-41 0.1714962780451259 1102 42-43 0.1183026028705072 1102 44-45 -0.010045523587329797 1102 46-47 0.01540497302841004 1102 48-49 0.17532249171790681 1102 50-51 0.3120374752053081 1102 52-53 0.04692251563130867 1102 54-55 -0.110846372116022 1102 56-57 -3.8125835404075517 1102 58-59 -10.142589702298398 1102 60-61 -8.402395394240472 1102 62-63 -2.5290753866643563 1102 64-65 -0.6571615829081061 1102 66-67 -1.6930093002388595 1102 68-69 -4.88289102354878 1102 70-71 -4.7800751193025555 1102 72-73 -3.309975030895826 1102 74-75 -4.03410370894461 1102 76-77 -4.3314822270101025 1102 78-79 1.5466191084289491 1102 80-81 2.0277611185726805 1102 82-83 0.7367649308867108 1102 84-85 0.5500636658240801 1102 86-87 0.46954018229759953 1102 88-89 0.2682102582600834 1102 90-91 1.6828170689458268 1102 92-93 1.1995024124222518 1102 94-95 -8.617401460524974 1102 96-97 -9.652062011539062 1102 98-99 -8.81813918185862 1102 100-101 -9.691483987634372 1103 1 0.2689657433057562 1103 2 0.2803258179720096 1103 3 0.292671365678725 1103 4 0.2559242202922647 1103 5 0.30203258317374804 1103 6 0.3276342322496646 1103 7 0.35881758362457106 1103 8 0.40196424284979315 1103 9 0.49138517635299905 1103 10-11 0.36901180951004164 1103 12-13 0.35796439566495764 1103 14-15 0.4518822579064121 1103 16-17 0.4218367560261669 1103 18-19 0.5174004429962693 1103 20-21 0.5224951312913717 1103 22-23 0.36115073276319976 1103 24-25 0.44188659261429564 1103 26-27 0.4424425963362779 1103 28-29 0.4345733607864375 1103 30-31 0.4398965019262562 1103 32-33 0.3992067688615535 1103 34-35 0.454229368732868 1103 36-37 0.44192700574471644 1103 38-39 0.4183331435638067 1103 40-41 0.4958362844315687 1103 42-43 0.3269119253214896 1103 44-45 0.37677708852698544 1103 46-47 0.28418804045519863 1103 48-49 0.31741721616333507 1103 50-51 0.24506256983014651 1103 52-53 0.30716646022744243 1103 54-55 0.2695326692142359 1103 56-57 0.7661002734715439 1103 58-59 -8.2017869877666 1103 60-61 -7.26309711945931 1103 62-63 -1.9944477852136409 1103 64-65 -0.11548325902748857 1103 66-67 -1.6122718636638709 1103 68-69 -3.630570261452622 1103 70-71 -3.8439954073956457 1103 72-73 -2.787140196650423 1103 74-75 -3.688653464619172 1103 76-77 -0.3283640748603922 1103 78-79 3.5923156922671424 1103 80-81 2.922576124080255 1103 82-83 1.4915906512659127 1103 84-85 0.9948192744698012 1103 86-87 0.8790839250469311 1103 88-89 0.7788412711853105 1103 90-91 2.070445310505761 1103 92-93 1.6339111756199252 1103 94-95 -7.13420084853955 1103 96-97 -8.664894590022017 1103 98-99 -8.116934143305588 1103 100-101 -9.603919390800643 1104 1 0.15924679996007285 1104 2 0.14140326708171003 1104 3 0.16963214981633357 1104 4 0.10708426295967399 1104 5 0.11865307711117623 1104 6 0.15419835964974737 1104 7 0.15696617155101222 1104 8 0.14320540605048393 1104 9 0.19344963805777837 1104 10-11 0.298026941782247 1104 12-13 0.35174230453791466 1104 14-15 0.369802821650822 1104 16-17 0.23527794229226373 1104 18-19 0.21610416353263417 1104 20-21 0.18382013832416533 1104 22-23 0.14909287811190808 1104 24-25 0.15501140197439867 1104 26-27 0.19900644709520066 1104 28-29 0.20971357654499911 1104 30-31 0.22916697400184205 1104 32-33 0.3258701817010774 1104 34-35 0.2937203633441072 1104 36-37 0.25224124856165986 1104 38-39 0.24169173627218754 1104 40-41 0.24558533136679728 1104 42-43 0.051689166303624745 1104 44-45 0.32397527031185547 1104 46-47 0.23404557025071426 1104 48-49 0.24373617481410292 1104 50-51 0.351216434776795 1104 52-53 0.36260193834421983 1104 54-55 0.22864941502302827 1104 56-57 0.56778690440958 1104 58-59 -8.378025738440687 1104 60-61 -7.447004489216106 1104 62-63 -2.3162089547806666 1104 64-65 -0.5065995384983069 1104 66-67 -1.7761916391605723 1104 68-69 -3.157530380932865 1104 70-71 -3.896739023182013 1104 72-73 -2.7835254485861007 1104 74-75 -3.666858810691892 1104 76-77 -0.34480419515978156 1104 78-79 3.4031368710531495 1104 80-81 2.8803412801543793 1104 82-83 1.3984016467825278 1104 84-85 0.9818649227783958 1104 86-87 0.8894471908355079 1104 88-89 0.7700978831788206 1104 90-91 2.247677903578449 1104 92-93 1.8446401646329598 1104 94-95 -6.703177103744984 1104 96-97 -7.97505982384309 1104 98-99 -5.935467592995876 1104 100-101 -5.390104056328889 1105 1 0.16905365671966166 1105 2 0.2863195878988698 1105 3 0.2926803437642924 1105 4 0.22395900355050458 1105 5 0.3032083532600609 1105 6 0.21195231792071212 1105 7 0.1521926901944468 1105 8 0.19428639954396232 1105 9 0.23502528009657198 1105 10-11 0.243265820379996 1105 12-13 0.27515484384380073 1105 14-15 0.3435799707929803 1105 16-17 0.3480796283240366 1105 18-19 0.4229468528753202 1105 20-21 0.39049303257409207 1105 22-23 0.46121293294002896 1105 24-25 0.4170228619970828 1105 26-27 0.4151707636073496 1105 28-29 0.46877160697129483 1105 30-31 0.29057046885385773 1105 32-33 0.3411108340668747 1105 34-35 0.27939030989480074 1105 36-37 0.3412471132806374 1105 38-39 0.35293802520030937 1105 40-41 0.2603121904820114 1105 42-43 0.26933438518765485 1105 44-45 0.29851834871981 1105 46-47 0.25555638688576465 1105 48-49 0.15958013753182598 1105 50-51 0.11765940566548494 1105 52-53 0.1820433706742719 1105 54-55 0.26719836696590704 1105 56-57 0.7040593678771856 1105 58-59 -8.754795759715325 1105 60-61 -7.840629891267255 1105 62-63 -2.2639348995061077 1105 64-65 -0.22655277167545762 1105 66-67 -1.5934816283733824 1105 68-69 -3.554680838427494 1105 70-71 -3.982530858982578 1105 72-73 -2.852199893736998 1105 74-75 -3.5474364384340475 1105 76-77 -0.07322214569245844 1105 78-79 3.7362866564201624 1105 80-81 3.08896267447712 1105 82-83 1.687855731756244 1105 84-85 1.3547606008849833 1105 86-87 1.1413727694523992 1105 88-89 1.048299296480529 1105 90-91 2.3223305289138523 1105 92-93 1.922171107716867 1105 94-95 -6.09152836694642 1105 96-97 -7.511448693043036 1105 98-99 -5.224898315716434 1105 100-101 -4.534179776829591 1106 1 0.1408804810694413 1106 2 0.14690978451734438 1106 3 0.25157801823034376 1106 4 0.10849766577666031 1106 5 0.11474189433776161 1106 6 0.20380856979159034 1106 7 0.16231908502060577 1106 8 0.22156718192346858 1106 9 0.2685225647517342 1106 10-11 0.15531812440433868 1106 12-13 0.1776375754888022 1106 14-15 0.20672071672400705 1106 16-17 0.17795146418838925 1106 18-19 0.11354685983999957 1106 20-21 0.11292319849169985 1106 22-23 0.21185778117161647 1106 24-25 0.20954336118858663 1106 26-27 0.18577662371512105 1106 28-29 0.27400237874549305 1106 30-31 0.3054462988552302 1106 32-33 0.19173503449200524 1106 34-35 0.22635332358812832 1106 36-37 0.33982893962308935 1106 38-39 0.2917260728288582 1106 40-41 0.3134430064713598 1106 42-43 0.23312421795449012 1106 44-45 0.1996223908005348 1106 46-47 0.13242537391563758 1106 48-49 0.13177216775993372 1106 50-51 0.14384439453431952 1106 52-53 0.3933756923706113 1106 54-55 0.19427221400399475 1106 56-57 0.9218321695453326 1106 58-59 -8.32683057466068 1106 60-61 -7.59203768825887 1106 62-63 -2.199702545905911 1106 64-65 -0.33549422160171005 1106 66-67 0.3391378431060872 1106 68-69 -2.375617661415994 1106 70-71 -2.2767712465844667 1106 72-73 -0.5281819924053721 1106 74-75 -0.7256000992092311 1106 76-77 2.366737761684366 1106 78-79 4.562071404972443 1106 80-81 3.5737292012522595 1106 82-83 1.967893805326451 1106 84-85 1.6104468379436092 1106 86-87 1.4784796037356394 1106 88-89 1.6023369275474906 1106 90-91 2.800227263148443 1106 92-93 2.1192829122472148 1106 94-95 -6.070297444622625 1106 96-97 -7.646852751620898 1106 98-99 -5.607878103729941 1106 100-101 -5.157858642586136 1107 1 0.0635043734664329 1107 2 0.12246884875267838 1107 3 0.08774579251448245 1107 4 0.08970889381419767 1107 5 0.13197206508104387 1107 6 -0.04223592210372118 1107 7 0.17012927200068617 1107 8 0.09402254546576927 1107 9 0.06217164224238303 1107 10-11 -0.01951767667086557 1107 12-13 0.048207120063473496 1107 14-15 -0.0395896450151767 1107 16-17 -0.00586152240808957 1107 18-19 -0.003623906694691925 1107 20-21 0.07165341314168217 1107 22-23 0.19362851598241093 1107 24-25 0.10268034420449368 1107 26-27 0.13285576928024767 1107 28-29 -0.04525098862102794 1107 30-31 0.0034764670994746893 1107 32-33 0.030512567726596274 1107 34-35 0.11769377617079613 1107 36-37 0.12440156426172422 1107 38-39 0.19244806911570578 1107 40-41 0.06667873965604798 1107 42-43 0.05426583390862305 1107 44-45 0.07249773151134775 1107 46-47 0.17330789519477463 1107 48-49 0.052282377543733105 1107 50-51 0.18573017532219893 1107 52-53 0.2420606084914496 1107 54-55 0.23907978636589178 1107 56-57 0.7265827881439364 1107 58-59 -8.591730913384414 1107 60-61 -7.92228135484698 1107 62-63 -2.429069605604102 1107 64-65 -0.5375207676296476 1107 66-67 0.5399239813604879 1107 68-69 -2.0692573328064228 1107 70-71 -2.357282952320002 1107 72-73 -1.1268243034043657 1107 74-75 -0.8771674297059207 1107 76-77 2.1924694504195017 1107 78-79 4.174707719669318 1107 80-81 3.177314487334808 1107 82-83 1.5545026424096768 1107 84-85 1.1234712497239379 1107 86-87 0.9285366824508152 1107 88-89 0.7874136636237736 1107 90-91 2.1319547333691133 1107 92-93 1.5643573610098294 1107 94-95 -7.116283729764909 1107 96-97 -8.47604716946486 1107 98-99 -6.694991781136803 1107 100-101 -6.147028698283441 1108 1 -0.04380449979664647 1108 2 -0.13021161859856178 1108 3 -0.07689966638794488 1108 4 -0.15036151352227023 1108 5 -0.09089927485035076 1108 6 -0.12503732754844776 1108 7 -0.057331075321002345 1108 8 -0.0029035592549249145 1108 9 -0.1382303814196959 1108 10-11 -0.04430952987397063 1108 12-13 -0.00784018991084423 1108 14-15 -0.12890236119618947 1108 16-17 -0.14733911388679388 1108 18-19 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52-53 -0.473784820048472 2313 54-55 -0.5227370047757134 2313 56-57 0.2793698741977124 2313 58-59 3.4555423308493154 2313 60-61 3.242273751757075 2313 62-63 0.3732887389807793 2313 64-65 -0.18489939831534485 2313 66-67 -2.108191621597598 2313 68-69 -4.504861829961303 2313 70-71 -3.3964383416202537 2313 72-73 -2.9161141116499927 2313 74-75 -3.74177090714236 2313 76-77 0.3589733818821621 2313 78-79 2.960296343062744 2313 80-81 2.2366875187302107 2313 82-83 0.7167936142869671 2313 84-85 0.3426110251060486 2313 86-87 0.39636041807156985 2313 88-89 0.4597365029516567 2313 90-91 2.1681405799702773 2313 92-93 3.5526992241996567 2313 94-95 5.76984776627631 2313 96-97 1.5450855874827027 2313 98-99 3.0526940309851547 2313 100-101 4.196358822374934 2314 1 -0.33020815379799373 2314 2 -0.2617980974624281 2314 3 -0.3156923151229378 2314 4 -0.18136528233513616 2314 5 -0.2086383199180304 2314 6 -0.32607712055492755 2314 7 -0.3628541748991978 2314 8 -0.4439089088060797 2314 9 -0.5145955153248707 2314 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98-99 6.295914635093222 2315 100-101 6.5433957200381005 2316 1 -0.4033010188509927 2316 2 -0.3794668540715591 2316 3 -0.5859550150621544 2316 4 -0.299405232709411 2316 5 -0.5693327470156717 2316 6 -0.6859097586285188 2316 7 -0.46112043856500406 2316 8 -0.5307053017818717 2316 9 -0.7154354890132453 2316 10-11 -0.6462282122823595 2316 12-13 -0.5294054703034021 2316 14-15 -0.7068590110855979 2316 16-17 -0.7787762686675848 2316 18-19 -0.65600925099222 2316 20-21 -0.6614926371828673 2316 22-23 -0.8511527595702049 2316 24-25 -0.7877503915045452 2316 26-27 -0.8123203031139852 2316 28-29 -0.7426177195463382 2316 30-31 -0.5456519849433334 2316 32-33 -0.5192026965231165 2316 34-35 -0.656256426593778 2316 36-37 -0.47977103897716944 2316 38-39 -0.8163636632073334 2316 40-41 -0.6913561256424998 2316 42-43 -0.4380440867669364 2316 44-45 -0.5981736918496594 2316 46-47 -0.873986430675977 2316 48-49 -0.7532763329161583 2316 50-51 -0.6617574673118654 2316 52-53 -0.638350967934251 2316 54-55 -0.4100073589651103 2316 56-57 0.23121838486198243 2316 58-59 3.399153638412411 2316 60-61 3.4450397629221072 2316 62-63 1.0535078858886386 2316 64-65 0.34386880097211403 2316 66-67 -1.6731450464816575 2316 68-69 -2.9920335562304388 2316 70-71 -3.143548774869476 2316 72-73 -0.7567106727641146 2316 74-75 5.485114404433773 2316 76-77 6.0036621158077565 2316 78-79 5.066700809076714 2316 80-81 3.7138913778182747 2316 82-83 1.7415571459736476 2316 84-85 1.3707369617390839 2316 86-87 1.1310102175921095 2316 88-89 1.1349708549972277 2316 90-91 2.8446092574850326 2316 92-93 4.327916153947935 2316 94-95 6.757781841090807 2316 96-97 3.847469735357649 2316 98-99 6.042427671620253 2316 100-101 6.1059442356795675 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 10.0 4 37.0 5 74.0 6 208.0 7 500.0 8 1132.0 9 1930.0 10 3119.0 11 5003.0 12 7230.0 13 9720.0 14 12673.0 15 16577.0 16 22497.0 17 29211.0 18 31889.0 19 31645.0 20 34202.0 21 34818.0 22 34785.0 23 35495.0 24 37283.0 25 39644.0 26 49539.0 27 68758.0 28 96333.0 29 127410.0 30 154494.0 31 174283.0 32 182546.0 33 183210.0 34 186718.0 35 198561.0 36 196396.0 37 145188.0 38 55579.0 39 3436.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.37062840937043 19.55879852700728 14.38514672049312 22.68542634312917 2 14.929929981974437 22.305354907342647 39.21981464460438 23.544900466078534 3 20.33001632361165 26.200685040185196 30.368020367672287 23.10127826853087 4 12.333096910363146 17.05875359437503 39.11814684171912 31.490002653542703 5 12.622030679156168 39.31374690894814 34.67695426622828 13.387268145667417 6 29.687944281931905 37.932053936984914 17.96041565237596 14.419586128707218 7 26.72055425163733 33.87409273209242 21.66792638427294 17.73742663199731 8 24.878804919769852 34.428850667409236 20.527083325799136 20.165261087021776 9 25.640229334801596 17.572103355262357 21.10217405160711 35.68549325832894 10-11 24.245152990072498 26.362133086693994 28.366145496413647 21.026568426819857 12-13 26.830594126855615 23.909120456825235 27.90328989569814 21.356995520621012 14-15 23.12214075185795 25.294174818725928 27.599632931305717 23.98405149811041 16-17 21.283126933934867 28.524072897901803 28.090464641624497 22.10233552653884 18-19 21.047791043021885 28.699827767807495 29.235103723560552 21.017277465610068 20-21 22.664464271426226 26.954049410140513 28.263281162061844 22.118205156371417 22-23 21.726050420927816 26.944917968462168 28.273587937817595 23.055443672792418 24-25 22.48595418953577 26.86974786592057 28.72987268190729 21.91442526263637 26-27 22.606390973789807 26.142905803012518 29.575380893259023 21.675322329938652 28-29 21.67536308536956 27.4565885401657 29.427482943378912 21.440565431085826 30-31 21.849296613855067 27.26653176697797 28.826693682770543 22.057477936396424 32-33 21.82149677036694 27.21836098834728 28.70334120548857 22.25680103579721 34-35 22.738984154179462 26.59487206147107 28.56386303044961 22.102280753899862 36-37 22.782882123729646 26.289479190820096 29.39225544477183 21.535383240678428 38-39 22.663488984994455 26.459922824499365 29.48840184643935 21.38818634406683 40-41 22.040298620231923 26.995578426267215 29.448934284456534 21.515188669044324 42-43 21.440915018929047 27.562502050128323 28.56484230695709 22.43174062398554 44-45 22.02296427088836 27.250723642611607 28.10672017681878 22.619591909681258 46-47 22.480673640662392 27.277307156606344 27.62330740951794 22.61871179321333 48-49 22.47779479534025 26.923608486282163 28.156179150824386 22.442417567553203 50-51 22.264878061799877 27.39589077471278 28.640537558576067 21.698693604911277 52-53 22.196186278245865 27.793353861045556 28.044254822561182 21.966205038147393 54-55 21.559664561811026 27.023854427568818 28.565048361025706 22.851432649594454 56-57 22.283649605319702 27.586908827720354 27.705131974758558 22.42430959220139 58-59 21.744312812769405 26.69462597201383 29.313631151610597 22.24743006360617 60-61 21.52342167728158 26.792851139184698 29.652617727545504 22.031109455988222 62-63 22.725163427574817 26.785237598072932 28.39032117267775 22.0992778016745 64-65 22.272476276432258 27.587938755753317 28.27728574764132 21.862299220173107 66-67 21.83160861970573 26.739220511259376 28.89798649711382 22.53118437192107 68-69 21.263740143852576 26.742339960245477 30.120432831172923 21.87348706472903 70-71 22.30517044157833 26.46129980654417 28.940233304308016 22.29329644756949 72-73 21.62183004324251 26.221903015695986 29.371688759825098 22.784578181236412 74-75 21.450806670232332 27.463375632844155 28.80617751141134 22.27964018551217 76-77 20.765263868113422 26.87245063282031 29.24922993826071 23.11305556080556 78-79 21.826066577697585 26.789377627844186 28.739764520611438 22.644791273846796 80-81 21.944056159099965 27.661513262174235 27.834082293178326 22.56034828554748 82-83 22.008594870065483 27.331781739212396 27.868209581383763 22.79141380933836 84-85 21.952979229083578 27.23398388188639 27.571987346907868 23.241049542122163 86-87 22.096695703118908 27.354629946279662 27.76310974458937 22.785564606012056 88-89 22.40370845192158 26.84328630789427 27.52936528314715 23.223639957036998 90-91 21.866180518216165 26.673412513121857 28.27372927460758 23.186677694054396 92-93 21.65638830279223 27.65393235050012 27.968615372090337 22.7210639746173 94-95 20.655741221390418 27.469897443878466 28.566587543294602 23.30777379143651 96-97 19.977439580894707 27.21148301429815 28.792183283639584 24.018894121167563 98-99 21.05748278978481 27.31448760839484 27.859359279321744 23.768670322498608 100-101 20.582580460961566 27.09624491265589 28.528520951310615 23.79265367507192 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 749.0 1 549.5 2 368.0 3 394.0 4 434.5 5 498.5 6 614.5 7 928.5 8 1349.0 9 1701.0 10 1814.5 11 1856.0 12 2021.5 13 2078.5 14 2241.5 15 2453.5 16 2628.0 17 2874.0 18 3091.5 19 3415.0 20 3870.5 21 4468.0 22 5262.0 23 6383.5 24 7733.0 25 9531.0 26 12127.0 27 15061.0 28 18103.0 29 21515.5 30 25320.0 31 29803.5 32 34668.5 33 39513.0 34 44672.0 35 49443.5 36 54663.0 37 59370.5 38 62393.5 39 64961.5 40 67072.0 41 69126.5 42 71326.5 43 74403.0 44 78555.0 45 84377.5 46 93572.0 47 107469.5 48 123547.5 49 118051.5 50 96330.5 51 83011.0 52 74558.5 53 68418.5 54 63187.5 55 57813.5 56 51994.0 57 45964.5 58 40222.0 59 34510.0 60 28402.5 61 22252.0 62 16813.0 63 12191.0 64 8660.0 65 6116.5 66 4290.5 67 3066.5 68 2164.0 69 1464.0 70 992.5 71 647.5 72 402.5 73 253.5 74 154.0 75 93.0 76 54.0 77 28.0 78 12.5 79 6.0 80 3.5 81 4.0 82 3.0 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02644501674172983 2 0.012567033596924604 3 1.808206272938792E-4 4 0.0 5 0.059670807006980124 6 0.0 7 4.52051568234698E-5 8 4.52051568234698E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 7.684876659989865E-4 16-17 9.04103136469396E-5 18-19 3.1643609776428854E-4 20-21 0.0 22-23 0.0 24-25 2.26025784117349E-5 26-27 6.780773523520469E-5 28-29 8.815005580576611E-4 30-31 0.0055602342892867845 32-33 0.005469823975639845 34-35 0.001785603694527057 36-37 0.08473706646559412 38-39 0.07777547231477978 40-41 0.08308707824153748 42-43 0.08631924695441558 44-45 0.08880553057970642 46-47 0.084917887092888 48-49 0.07574124025772364 50-51 0.07006799307637819 52-53 0.05897012707621634 54-55 0.04787226107605451 56-57 0.06722006819649959 58-59 0.7110997194115916 60-61 0.34057565150802144 62-63 0.00323216871287809 64-65 0.001966424321820936 66-67 1.1022147362482522 68-69 2.379350826824921 70-71 2.3485887176065496 72-73 1.812026108690375 74-75 1.2813401701612512 76-77 0.7400762249354358 78-79 0.5649062422444903 80-81 0.03833397298630239 82-83 1.5821804888214427E-4 84-85 0.001333552126292359 86-87 0.0014691675967627683 88-89 4.52051568234698E-5 90-91 0.331059965996681 92-93 0.053432495365341295 94-95 0.9512973201930983 96-97 1.6565655743744623 98-99 0.637234493162042 100-101 0.5678219748596041 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2212137.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.8501501603804 #Duplication Level Percentage of deduplicated Percentage of total 1 72.99690198504052 36.389065251968404 2 14.461752757264609 14.41841093063872 3 5.262070580110204 7.869450257190409 4 2.515103487720185 5.015131461269908 5 1.3299211747027526 3.314838513020086 6 0.8606972163139968 2.574353128552449 7 0.5292795520753386 1.8469265603442155 8 0.37293472646102876 1.487268169128214 9 0.2564387821029118 1.15051606152977 >10 1.292440659177414 11.279622342885167 >50 0.07690615134989351 2.660737757836952 >100 0.03926948399488329 3.5878410395652636 >500 0.0020034168274888843 0.6617791088798144 >1k 0.0033693828462312775 3.9120233562098026 >5k 6.374508087464578E-4 2.0692286555741184 >10k+ 2.731932037484819E-4 1.762807405406831 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15071 0.6812869184865132 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 12713 0.5746931586967715 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 11048 0.4994265725856943 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8273 0.3739822624005656 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7807 0.3529166593208287 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6700 0.3028745507172476 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6322 0.28578700143797603 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5927 0.2679309644927055 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5502 0.2487187728427308 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5051 0.22833124711534591 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4991 0.22561893770593772 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4770 0.21562859804795093 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4497 0.20328759023514364 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4289 0.19388491761586193 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4185 0.18918358130622107 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3900 0.1763001116115322 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3819 0.17263849390883115 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3513 0.1588057159208494 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3407 0.1540139692975616 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2937 0.13276754559053078 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2776 0.12548951534195216 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2772 0.12530869471465828 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2691 0.12164707701195722 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2324 0.1050567844577438 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2245 0.1014855770686897 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 4.52051568234698E-5 0.0 0.0 0.0 5 0.0 9.04103136469396E-5 0.0 0.0 0.0 6 0.0 9.04103136469396E-5 0.0 0.0 0.0 7 0.0 9.04103136469396E-5 0.0 0.0 0.0 8 0.0 9.04103136469396E-5 0.0 0.0 0.0 9 0.0 9.04103136469396E-5 0.0 0.0 0.0 10-11 0.0 9.04103136469396E-5 0.0 4.52051568234698E-5 0.0 12-13 0.0 9.04103136469396E-5 0.0 1.3561547047040938E-4 0.0 14-15 0.0 1.130128920586745E-4 0.0 2.0342320570561408E-4 0.0 16-17 0.0 1.3561547047040938E-4 0.0 7.006799307637819E-4 0.0 18-19 0.0 1.3561547047040938E-4 0.0 7.910902444107214E-4 0.0 20-21 0.0 1.3561547047040938E-4 0.0 0.00106232118535154 0.0 22-23 0.0 1.3561547047040938E-4 0.0 0.0017177959592918522 0.0 24-25 0.0 1.3561547047040938E-4 0.0 0.002395873311643899 0.0 26-27 0.0 1.3561547047040938E-4 0.0 0.003299976448113295 0.0 28-29 0.0 1.3561547047040938E-4 0.0 0.0052211956131107615 0.0 30-31 0.0 1.808206272938792E-4 0.0 0.01089444279445622 0.0 32-33 0.0 1.808206272938792E-4 0.0 0.023936130538027255 0.0 34-35 0.0 1.808206272938792E-4 0.0 0.04599624706788052 4.52051568234698E-5 36-37 0.0 1.808206272938792E-4 0.0 0.07583165057137059 4.52051568234698E-5 38-39 0.0 2.2602578411734896E-4 0.0 0.1189799727593725 4.52051568234698E-5 40-41 0.0 2.2602578411734896E-4 0.0 0.16857002979471886 4.52051568234698E-5 42-43 0.0 2.2602578411734896E-4 0.0 0.22039774209282698 4.52051568234698E-5 44-45 0.0 2.2602578411734896E-4 0.0 0.2771528164846933 4.52051568234698E-5 46-47 0.0 2.2602578411734896E-4 0.0 0.35076941437171383 4.52051568234698E-5 48-49 0.0 2.2602578411734896E-4 0.0 0.42413738389620537 4.52051568234698E-5 50-51 0.0 2.2602578411734896E-4 0.0 0.5043765372578642 4.52051568234698E-5 52-53 0.0 2.2602578411734896E-4 0.0 0.5821972147294674 4.52051568234698E-5 54-55 0.0 2.2602578411734896E-4 0.0 0.6639507408447125 4.52051568234698E-5 56-57 0.0 2.486283625290839E-4 0.0 0.7430823678641965 4.52051568234698E-5 58-59 0.0 2.7123094094081877E-4 0.0 0.8046291888793506 9.04103136469396E-5 60-61 0.0 2.9383351935255366E-4 0.0 0.862310968986098 9.04103136469396E-5 62-63 4.52051568234698E-5 3.3903867617602343E-4 0.0 0.9127373214226786 9.04103136469396E-5 64-65 4.52051568234698E-5 3.616412545877584E-4 0.0 0.9637287383195525 9.04103136469396E-5 66-67 4.52051568234698E-5 3.616412545877584E-4 0.0 1.008368830682729 9.04103136469396E-5 68-69 4.52051568234698E-5 3.616412545877584E-4 0.0 1.0643328148301845 9.04103136469396E-5 70-71 4.52051568234698E-5 3.616412545877584E-4 0.0 1.151533562342658 9.04103136469396E-5 72-73 4.52051568234698E-5 3.616412545877584E-4 0.0 1.2724573568454396 9.04103136469396E-5 74-75 4.52051568234698E-5 3.616412545877584E-4 0.0 1.4296582896990557 9.04103136469396E-5 76-77 4.52051568234698E-5 3.616412545877584E-4 0.0 1.617621331771043 9.04103136469396E-5 78-79 4.52051568234698E-5 3.616412545877584E-4 0.0 1.872555813677001 9.04103136469396E-5 80-81 4.52051568234698E-5 3.616412545877584E-4 0.0 2.1743002354736616 9.04103136469396E-5 82-83 4.52051568234698E-5 3.616412545877584E-4 0.0 2.470936474549271 9.04103136469396E-5 84-85 4.52051568234698E-5 3.8424383299949326E-4 0.0 2.7619446716003573 9.04103136469396E-5 86-87 4.52051568234698E-5 4.0684641141122815E-4 0.0 3.0337406770014694 9.04103136469396E-5 88-89 4.52051568234698E-5 4.0684641141122815E-4 0.0 3.2811032951394963 9.04103136469396E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 9170 0.0 25.418104 2 GTACATG 9635 0.0 24.979431 1 ACATGGG 9255 0.0 23.856453 3 CATGGGA 6310 0.0 22.355185 4 GAGTACT 6990 0.0 20.811258 12-13 ATGGGAT 2515 0.0 19.884024 5 GTACTTT 7450 0.0 19.368034 14-15 AGTACTT 7325 0.0 18.861681 12-13 GGTATCG 270 0.0 17.941431 62-63 TTACGCG 150 6.148184E-8 17.762018 62-63 CATGGGG 3510 0.0 17.473207 4 AGTACAT 7150 0.0 16.497551 2 ATGGGAG 2835 0.0 16.47474 5 ACTTTTT 8900 0.0 16.26555 16-17 GCTTATA 1800 0.0 16.254862 1 GTGTAGC 1545 0.0 16.188683 1 TATACTC 1825 0.0 16.027481 4 CGCATAG 835 0.0 15.707789 66-67 GTATCGA 310 0.0 15.62641 62-63 GCGTTTA 860 0.0 15.490132 62-63 >>END_MODULE