FastQCFastQC Report
Fri 27 May 2016
SRR939573_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939573_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375251
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86970.366150777328375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59350.24986832970494485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43420.1828017333747044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39250.051.877041
GTATCAA70500.041.4041181
TCAACGC87500.033.575454
ATCAACG87750.033.265553
CAACGCA90000.032.5900655
TATCAAC89750.032.577142
AACGCAG93850.031.295786
ACGCAGA106500.027.1329577
CGCAGAG105100.027.0440568
TACATGG68300.023.7668782
GTACATG70400.023.6642111
GCAGAGT114700.023.4153569
ACATGGG68300.022.3075083
GAGTACT69700.021.51350612-13
AGAGTAC97700.021.03008810-11
CATGGGG39500.020.7868294
CAGAGTA106950.020.29822310-11
GTACTTT75850.019.33266814-15
AGTACTT75450.019.30794312-13
TACCGTA1507.780088E-418.9797047
Produced by FastQC (version 0.11.5)