##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939573_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2375251 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.14267502676559 31.0 31.0 34.0 30.0 34.0 2 31.38268039882943 31.0 31.0 34.0 28.0 34.0 3 31.46304958928551 31.0 31.0 34.0 28.0 34.0 4 35.06395618820916 37.0 35.0 37.0 32.0 37.0 5 35.057776209756355 37.0 35.0 37.0 32.0 37.0 6 35.18024410893838 37.0 35.0 37.0 32.0 37.0 7 35.05301334469494 37.0 35.0 37.0 32.0 37.0 8 35.05194693108223 37.0 35.0 37.0 32.0 37.0 9 36.48911251905588 39.0 37.0 39.0 32.0 39.0 10-11 36.40649872371383 39.0 36.0 39.0 32.0 39.0 12-13 36.27960645001307 38.0 35.0 39.0 32.0 39.0 14-15 37.18089119844598 39.0 36.5 41.0 32.0 41.0 16-17 36.92314896404633 39.0 36.0 40.0 31.0 41.0 18-19 36.87272818746314 39.0 36.0 40.0 31.0 41.0 20-21 37.01966192204529 39.0 36.0 41.0 31.0 41.0 22-23 36.89879448529861 39.0 36.0 41.0 31.0 41.0 24-25 36.81274063246369 39.0 36.0 41.0 31.0 41.0 26-27 36.97819809359095 39.0 36.0 41.0 31.0 41.0 28-29 36.881373800074186 39.0 36.0 41.0 31.0 41.0 30-31 36.70106127731343 39.0 36.0 41.0 30.0 41.0 32-33 36.504660139075824 39.0 36.0 40.0 30.0 41.0 34-35 36.313680954139166 39.0 35.0 40.0 30.0 41.0 36-37 36.08132656296114 39.0 35.0 40.0 29.5 41.0 38-39 35.94285214488911 38.0 35.0 40.0 29.0 41.0 40-41 35.70280846108474 38.0 35.0 40.0 28.0 41.0 42-43 35.42457533961674 38.0 35.0 40.0 27.0 41.0 44-45 35.19309201427554 38.0 34.0 40.0 26.5 41.0 46-47 34.79830573695159 38.0 34.0 40.0 24.5 41.0 48-49 34.464521223230726 38.0 33.5 40.0 24.0 41.0 50-51 33.897537565503605 37.5 33.0 39.5 23.5 40.5 52-53 33.98678918564817 38.0 33.0 39.5 24.0 40.0 54-55 34.06051844626104 38.0 33.0 40.0 23.0 41.0 56-57 33.936822466341454 38.0 33.0 40.0 23.0 41.0 58-59 33.56623826281938 37.0 33.0 40.0 22.0 41.0 60-61 33.18217358923331 37.0 32.0 39.0 21.0 40.5 62-63 32.78874022155974 36.0 31.5 39.0 19.5 40.0 64-65 32.206309775261644 36.0 31.0 39.0 17.5 40.0 66-67 31.703582274041775 35.0 31.0 38.0 14.0 40.0 68-69 31.2016544777794 35.0 30.0 38.0 10.0 40.0 70-71 30.66761975892232 34.0 29.5 37.5 8.0 39.0 72-73 29.94085593480436 34.0 29.0 36.5 7.0 39.0 74-75 29.498482265663714 34.0 28.0 36.0 2.0 39.0 76-77 29.13445421136545 34.0 28.0 36.0 2.0 38.0 78-79 28.572687475976224 33.0 27.0 35.0 2.0 37.5 80-81 27.924129281494885 33.0 26.0 35.0 2.0 37.0 82-83 27.28721596159732 32.0 26.0 35.0 2.0 36.0 84-85 26.651212440285256 32.0 25.0 34.0 2.0 36.0 86-87 26.01024628555045 31.5 23.5 34.0 2.0 35.0 88-89 25.222154837530855 31.0 20.5 34.0 2.0 35.0 90-91 23.921764057777473 30.5 12.5 34.0 2.0 35.0 92-93 22.16563702109798 29.0 6.0 34.0 2.0 35.0 94-95 22.007603406966254 29.0 2.0 34.0 2.0 35.0 96-97 21.432983082630003 29.0 2.0 33.5 2.0 34.0 98-99 20.41073090801772 28.5 2.0 33.0 2.0 34.0 100-101 18.5692099487591 25.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7893124980788784 1101 2 0.7413180528466654 1101 3 0.6533370524366582 1101 4 0.7413237712292045 1101 5 0.5095610474833094 1101 6 0.5274909930157605 1101 7 0.5539230749823929 1101 8 0.6662194634084102 1101 9 0.8757648428769897 1101 10-11 0.5817168039192282 1101 12-13 0.4728527486051064 1101 14-15 0.7565922551440565 1101 16-17 0.5487703873227332 1101 18-19 0.6368625869888049 1101 20-21 0.8192818792748469 1101 22-23 0.5820857496964678 1101 24-25 0.6208727347584357 1101 26-27 0.6236737149237541 1101 28-29 0.7904442252791455 1101 30-31 0.7704011476444848 1101 32-33 0.6652339607052795 1101 34-35 0.8202585900828652 1101 36-37 0.8927561530183681 1101 38-39 0.7748158987957652 1101 40-41 0.9083514653043636 1101 42-43 0.8554865605083535 1101 44-45 0.7532538869081478 1101 46-47 0.7157571110603556 1101 48-49 0.6212005305229766 1101 50-51 0.8616478423079386 1101 52-53 0.9697052609603958 1101 54-55 0.8840488248606704 1101 56-57 0.8343879750361154 1101 58-59 0.773948059389447 1101 60-61 0.600606911850015 1101 62-63 0.5085156922405076 1101 64-65 0.45654788776415245 1101 66-67 0.4210733228424637 1101 68-69 0.19672688156942897 1101 70-71 0.17200636137248893 1101 72-73 0.575616752139755 1101 74-75 0.3628808791463989 1101 76-77 -0.025928730166302927 1101 78-79 0.1318475651697142 1101 80-81 -0.016984780422514945 1101 82-83 -0.22552673652976907 1101 84-85 -0.2958639573535429 1101 86-87 -0.8005352399184069 1101 88-89 -1.036088957918988 1101 90-91 -4.778745609639568 1101 92-93 -10.654082242633812 1101 94-95 -8.8050722716138 1101 96-97 -5.312157318444733 1101 98-99 -3.9602513421681387 1101 100-101 -3.397389901166221 1102 1 0.693157535803774 1102 2 0.5522629274072735 1102 3 0.5916012690837995 1102 4 0.5869758261137292 1102 5 0.4699852198899279 1102 6 0.5289531750370315 1102 7 0.49578156007311946 1102 8 0.4852475408074213 1102 9 0.6438161246474863 1102 10-11 0.5270920817913023 1102 12-13 0.5726956071558931 1102 14-15 0.6657542312935973 1102 16-17 0.7973659698411524 1102 18-19 0.667941278170268 1102 20-21 0.7241472413572225 1102 22-23 0.6745253022539828 1102 24-25 0.7976961504769662 1102 26-27 0.674716077411297 1102 28-29 0.7520988371342909 1102 30-31 0.5776622324132603 1102 32-33 0.6611768915301894 1102 34-35 0.7015831227650011 1102 36-37 0.7055286866641595 1102 38-39 0.6074916015520415 1102 40-41 0.739834645146459 1102 42-43 0.6387069199078184 1102 44-45 0.421063981193015 1102 46-47 0.6402036939182523 1102 48-49 0.6090613535718532 1102 50-51 0.6171727056989127 1102 52-53 0.6726449558760237 1102 54-55 0.787622150236551 1102 56-57 0.704782279616758 1102 58-59 0.7392070690150447 1102 60-61 0.7573622146895147 1102 62-63 0.7444630306914348 1102 64-65 0.8095282368700296 1102 66-67 0.7026880470510015 1102 68-69 0.5654313680399348 1102 70-71 0.5043725721281902 1102 72-73 0.43188885871122196 1102 74-75 0.34187566664954616 1102 76-77 0.25736185010724455 1102 78-79 0.2814934493778196 1102 80-81 0.2779500086137183 1102 82-83 0.203561952571075 1102 84-85 -0.4403301965808666 1102 86-87 -0.6485297700819039 1102 88-89 -1.28174361357663 1102 90-91 -5.936277722343387 1102 92-93 -11.915969007710082 1102 94-95 -10.579101909476677 1102 96-97 -6.481667770814994 1102 98-99 -4.925453819199205 1102 100-101 -4.281991271089238 1103 1 0.686953864783657 1103 2 0.6629901050774549 1103 3 0.6639437422271435 1103 4 0.7394969884258344 1103 5 0.5698278761846751 1103 6 0.5066435026449412 1103 7 0.5899258767185813 1103 8 0.5683844581362081 1103 9 0.6018682928270991 1103 10-11 0.6191175032060272 1103 12-13 0.4812894039843769 1103 14-15 0.6061108936756341 1103 16-17 0.7852413469665436 1103 18-19 0.737423425903522 1103 20-21 0.6530618528431447 1103 22-23 0.6929745593252932 1103 24-25 0.6342770634367554 1103 26-27 0.5964703847992965 1103 28-29 0.7635194093658768 1103 30-31 0.8573142780479301 1103 32-33 0.8467216670531741 1103 34-35 0.8736660204199964 1103 36-37 0.9244257489156524 1103 38-39 0.7892428120163117 1103 40-41 0.6051461856828553 1103 42-43 0.699470561357316 1103 44-45 0.5895016664256829 1103 46-47 0.7022959322947955 1103 48-49 0.7067037787628223 1103 50-51 0.707376270158214 1103 52-53 0.854545934237521 1103 54-55 0.8614252558183395 1103 56-57 0.6667473110189022 1103 58-59 0.5211013040748824 1103 60-61 0.45286060295715913 1103 62-63 0.47689731263803026 1103 64-65 0.3465651968231427 1103 66-67 0.5723706623198304 1103 68-69 0.472134209883702 1103 70-71 0.7266228035649647 1103 72-73 0.5259884697642434 1103 74-75 0.46511608117112146 1103 76-77 0.27167471599379667 1103 78-79 0.20076605525598268 1103 80-81 0.1761368097246141 1103 82-83 0.016904365773960706 1103 84-85 -0.12394355730921092 1103 86-87 -0.32328193293346885 1103 88-89 -0.5360022999481657 1103 90-91 -2.2954514431140893 1103 92-93 -10.293503240372305 1103 94-95 -6.223706685399261 1103 96-97 -3.1429923550613097 1103 98-99 -2.0621708469516378 1103 100-101 -1.65302579346368 1104 1 0.7170764547226938 1104 2 0.6082522185852426 1104 3 0.5950025949379558 1104 4 0.5350397390833024 1104 5 0.583737557132828 1104 6 0.4948234793035624 1104 7 0.5649104059000791 1104 8 0.5404838148172786 1104 9 0.6030180624343302 1104 10-11 0.4709566066935551 1104 12-13 0.538259095172215 1104 14-15 0.5154988343872589 1104 16-17 0.6629345572965377 1104 18-19 0.5148705659486126 1104 20-21 0.6218932892919469 1104 22-23 0.5222190809383278 1104 24-25 0.4022911700248102 1104 26-27 0.37343314353402235 1104 28-29 0.5047883662860855 1104 30-31 0.5775406957822895 1104 32-33 0.5885264580778653 1104 34-35 0.4516128694928554 1104 36-37 0.4370984639886544 1104 38-39 0.3917271106460447 1104 40-41 0.2880987110992024 1104 42-43 0.2402673482607014 1104 44-45 0.22630790436146953 1104 46-47 0.024553940391136564 1104 48-49 0.07663359562521777 1104 50-51 0.1220520189744505 1104 52-53 0.19678060095506567 1104 54-55 0.2910825445931735 1104 56-57 0.15004022624263058 1104 58-59 -0.02354280349897664 1104 60-61 0.09559313428835736 1104 62-63 -0.07119139732148483 1104 64-65 -0.07820973695285005 1104 66-67 -0.07791283685176964 1104 68-69 -0.03108486517441378 1104 70-71 0.03536526293220277 1104 72-73 0.036069973788563914 1104 74-75 -0.031594501631939664 1104 76-77 -0.2005138085703031 1104 78-79 -0.4054082164389392 1104 80-81 -0.405801478722676 1104 82-83 -0.47957420245187166 1104 84-85 -0.5614040534566094 1104 86-87 -0.6309328807397314 1104 88-89 -0.794606523656519 1104 90-91 -1.1207524801200321 1104 92-93 -5.020009888213227 1104 94-95 -2.435154518689661 1104 96-97 -1.5504957497373155 1104 98-99 -1.213078330502963 1104 100-101 -1.1862335794353207 1105 1 0.7867087725528918 1105 2 0.6619319053391379 1105 3 0.6191531058729218 1105 4 0.6325388636495646 1105 5 0.5796475307106448 1105 6 0.4755212795773289 1105 7 0.5418129621665884 1105 8 0.5594574881372409 1105 9 0.7655125710772523 1105 10-11 0.6157396582963983 1105 12-13 0.6265725322842428 1105 14-15 0.7971621584887458 1105 16-17 0.7740910848488198 1105 18-19 0.8228032689696576 1105 20-21 0.6003356539180587 1105 22-23 0.6491611113079188 1105 24-25 0.7074476339091973 1105 26-27 0.6659063234276985 1105 28-29 0.7006862429613463 1105 30-31 0.7436849672622543 1105 32-33 0.7888779049310841 1105 34-35 0.6817134647877978 1105 36-37 0.8379325135859546 1105 38-39 0.89010294230809 1105 40-41 0.8613852485200155 1105 42-43 0.9546346484230597 1105 44-45 0.7347582274760995 1105 46-47 0.7060689182665385 1105 48-49 0.614914243764531 1105 50-51 0.6905959339141248 1105 52-53 0.6219615769840985 1105 54-55 0.7123262971796791 1105 56-57 0.725787846654562 1105 58-59 0.6415980617478922 1105 60-61 0.6553995838474549 1105 62-63 0.6332739514194756 1105 64-65 0.7149077621717126 1105 66-67 0.6063166222893521 1105 68-69 0.5481985525188975 1105 70-71 0.3689968804154127 1105 72-73 0.5848615568911022 1105 74-75 0.39907114634819507 1105 76-77 0.22958583170601798 1105 78-79 -0.05436310772598674 1105 80-81 -0.09471816926480514 1105 82-83 0.018179593747152722 1105 84-85 0.04081604650200532 1105 86-87 -0.33115444506023195 1105 88-89 -0.48420127742088326 1105 90-91 -1.638479535797618 1105 92-93 -8.539114717424063 1105 94-95 -3.6567595866551557 1105 96-97 -1.867995192643118 1105 98-99 -1.157417616785711 1105 100-101 -0.7672694563015838 1106 1 0.6331525705604797 1106 2 0.6021387963248515 1106 3 0.6253152710698977 1106 4 0.6790314368386561 1106 5 0.622244405590024 1106 6 0.48195714723166816 1106 7 0.506233397213137 1106 8 0.5202773322672058 1106 9 0.5636026191857226 1106 10-11 0.5316352794079862 1106 12-13 0.4432020619721797 1106 14-15 0.49702285095596466 1106 16-17 0.633761203207591 1106 18-19 0.5646890438107874 1106 20-21 0.6606417795895894 1106 22-23 0.6453917372092945 1106 24-25 0.6019290203875869 1106 26-27 0.46092511415410087 1106 28-29 0.691138062556341 1106 30-31 0.7394546852434516 1106 32-33 0.5334087683921567 1106 34-35 0.6380031823479229 1106 36-37 0.8808266012619725 1106 38-39 0.6549469143623696 1106 40-41 0.642255201253704 1106 42-43 0.8016165524798211 1106 44-45 0.6688296217093992 1106 46-47 0.4805835350304619 1106 48-49 0.26495757998694813 1106 50-51 0.13497310291837294 1106 52-53 0.33550493601505593 1106 54-55 0.42355809859802207 1106 56-57 0.2946655963992839 1106 58-59 0.27834559930303016 1106 60-61 0.3622902186617054 1106 62-63 0.2512405877917345 1106 64-65 0.30127351988890183 1106 66-67 0.3076284641314402 1106 68-69 0.061533652051849685 1106 70-71 0.10859302893057787 1106 72-73 0.2485596630806377 1106 74-75 0.28306235979751193 1106 76-77 0.11355599112015469 1106 78-79 0.05069652782866285 1106 80-81 0.19036311685602314 1106 82-83 0.20246959904575235 1106 84-85 -0.1350546255778866 1106 86-87 -0.059738806824700674 1106 88-89 -0.16934291844256677 1106 90-91 -0.5045249914595722 1106 92-93 -4.895276356270159 1106 94-95 -2.2171729124558226 1106 96-97 -1.0938077906759673 1106 98-99 -0.8676335610065529 1106 100-101 -0.45789742799092537 1107 1 0.5141619753154281 1107 2 0.3711770968486334 1107 3 0.4277277951659322 1107 4 0.44946195263980826 1107 5 0.28886239340084074 1107 6 0.2655698971975937 1107 7 0.29645673580378684 1107 8 0.299518720977602 1107 9 0.3728301831010583 1107 10-11 0.2478375497898213 1107 12-13 0.21777514840636059 1107 14-15 0.42714435338016443 1107 16-17 0.4693970464227277 1107 18-19 0.3198928135013759 1107 20-21 0.2839696608910671 1107 22-23 0.37493688198021147 1107 24-25 0.4036016258746713 1107 26-27 0.27577508592036537 1107 28-29 0.2719887742412084 1107 30-31 0.24740881028969852 1107 32-33 0.23897470877029292 1107 34-35 0.22563972653858855 1107 36-37 0.2464924306523173 1107 38-39 0.14322504587316587 1107 40-41 0.2024271349725737 1107 42-43 0.02076389364071929 1107 44-45 0.0732097451589766 1107 46-47 0.10186203650056314 1107 48-49 0.09727352523297128 1107 50-51 0.0797420730685019 1107 52-53 0.073958311406912 1107 54-55 0.1028228531592319 1107 56-57 -0.01909396899589666 1107 58-59 -0.028749830725985248 1107 60-61 -0.027150363926843113 1107 62-63 2.6214278910430266E-4 1107 64-65 -0.017076461613136473 1107 66-67 -0.03581546756239007 1107 68-69 -0.04234702633668519 1107 70-71 -0.06331148262578523 1107 72-73 -0.054057312209973674 1107 74-75 -0.08284686387209561 1107 76-77 -0.17967570076215722 1107 78-79 -0.24836689098474096 1107 80-81 -0.22946767544103608 1107 82-83 -0.1037447436923351 1107 84-85 -0.1679615321339547 1107 86-87 -0.3350956228381001 1107 88-89 -0.41587698390589267 1107 90-91 -0.7651807930056158 1107 92-93 -4.760202267051554 1107 94-95 -2.020310751504052 1107 96-97 -1.0728904863162896 1107 98-99 -1.0323628092103014 1107 100-101 -0.8474694434902723 1108 1 0.37172208300994214 1108 2 0.3691249988799896 1108 3 0.08831301518479151 1108 4 -0.10206696299449902 1108 5 -0.7981965156682946 1108 6 5.340453609505857E-4 1108 7 0.12981380641041795 1108 8 0.1498011089900686 1108 9 0.30513960594316103 1108 10-11 0.19456854232861787 1108 12-13 0.1353033070141123 1108 14-15 0.1984254604352742 1108 16-17 0.3560637558592603 1108 18-19 0.19133986762022914 1108 20-21 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0.014296770859345997 2312 72-73 0.21783744193157517 2312 74-75 0.31719362750553515 2312 76-77 0.48088149701714755 2312 78-79 0.8169660275946953 2312 80-81 0.7211743339299943 2312 82-83 0.9550667425820869 2312 84-85 0.7301227571968383 2312 86-87 0.6801358616827251 2312 88-89 0.8663960907085411 2312 90-91 1.4313417657733396 2312 92-93 2.576316696410302 2312 94-95 1.8987004716718765 2312 96-97 1.6578516925839253 2312 98-99 1.626503943315118 2312 100-101 1.5864602895030906 2313 1 -0.18600072015100722 2313 2 -0.6116855876101326 2313 3 -0.17304593805203794 2313 4 0.043066958874753425 2313 5 -0.5588081698618268 2313 6 -0.21264290512689143 2313 7 -0.8963880989026691 2313 8 -0.6322269985394726 2313 9 -0.6526953659718728 2313 10-11 -1.789658057223484 2313 12-13 -0.45057089751728086 2313 14-15 -0.08480173263111368 2313 16-17 -1.3842063825937032 2313 18-19 -0.8781082263277185 2313 20-21 -1.1292138695423048 2313 22-23 -0.983673618381907 2313 24-25 -0.16031500498345963 2313 26-27 -0.8169572226092683 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3.0538724963918042 2313 94-95 2.0759653698706764 2313 96-97 1.6661371285992388 2313 98-99 1.6484043552510208 2313 100-101 1.6886906059531377 2314 1 -0.41418918724062337 2314 2 -0.5446944701754006 2314 3 -1.5196377113302582 2314 4 -0.3445930438052969 2314 5 -0.1556769525142414 2314 6 -2.201956514220619 2314 7 -2.9478709589743133 2314 8 -0.9716065419242383 2314 9 -1.3572539220237019 2314 10-11 -0.642933647196692 2314 12-13 -1.388860497993889 2314 14-15 -2.4671185398580207 2314 16-17 -1.80614035270861 2314 18-19 -2.2680763132773407 2314 20-21 -1.7429475769442675 2314 22-23 -3.0843028320952115 2314 24-25 -1.6340766565755729 2314 26-27 -1.5096276339453567 2314 28-29 -0.8350371674929349 2314 30-31 -1.0334122021473533 2314 32-33 -1.6255253103925043 2314 34-35 -0.3849869438511391 2314 36-37 -0.20687807068555486 2314 38-39 -0.228207369288441 2314 40-41 -0.3183542534932968 2314 42-43 -0.18431753260324513 2314 44-45 -0.7234511570255009 2314 46-47 -1.119184893580254 2314 48-49 -1.3921308559299277 2314 50-51 -0.6679653133684624 2314 52-53 -0.21896021385551734 2314 54-55 -0.008641661642663223 2314 56-57 0.07571799703668347 2314 58-59 -0.1081128280690038 2314 60-61 -0.056902913556527324 2314 62-63 -0.15411389698817146 2314 64-65 -0.2181807862262204 2314 66-67 -0.022148404343148798 2314 68-69 0.014580715840004643 2314 70-71 -0.10538250386207437 2314 72-73 0.11025506797365736 2314 74-75 0.39259268767152733 2314 76-77 0.20279495473846154 2314 78-79 0.2705661088377127 2314 80-81 0.217339294185539 2314 82-83 0.3763071757550911 2314 84-85 0.4209062907141998 2314 86-87 0.702275507352379 2314 88-89 0.7194376262015822 2314 90-91 1.5110845577635175 2314 92-93 2.6515052633019245 2314 94-95 2.0459783689548523 2314 96-97 1.6723341837902908 2314 98-99 1.8667746302802293 2314 100-101 1.8663009424015264 2315 1 -7.403671627267837 2315 2 -2.775358069874052 2315 3 -1.287467381509586 2315 4 -0.7159414578167898 2315 5 -0.8222262669155498 2315 6 -0.9638891418594469 2315 7 -2.273773765760801 2315 8 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72-73 -1.4517105763906066 2315 74-75 -2.028152578259043 2315 76-77 -2.0011818277542837 2315 78-79 -1.1143042047482155 2315 80-81 -0.8177644166390188 2315 82-83 -1.4056465388089343 2315 84-85 -1.276893719136094 2315 86-87 -1.7189236929496978 2315 88-89 -1.3249870163148358 2315 90-91 -0.11113934671052306 2315 92-93 0.8269386306248521 2315 94-95 0.6143791737603372 2315 96-97 0.5149188279119485 2315 98-99 0.36515088231641357 2315 100-101 0.33037564411091935 2316 1 -3.6208724872909137 2316 2 -2.271338318834392 2316 3 -3.911577518662945 2316 4 -3.399982211273521 2316 5 -3.3648191288834433 2316 6 -3.6090909663198936 2316 7 -2.313165452868624 2316 8 -1.0987288851806412 2316 9 -1.4021826116808924 2316 10-11 -2.427859114339249 2316 12-13 -3.3196286771266443 2316 14-15 -2.218684633144939 2316 16-17 -2.1025033055085345 2316 18-19 -2.2670460851135346 2316 20-21 -4.785308508348649 2316 22-23 -5.44200537446396 2316 24-25 -3.381493029879479 2316 26-27 -2.7969128606687903 2316 28-29 -3.6512351861346914 2316 30-31 -4.995941192572577 2316 32-33 -3.3624641167926157 2316 34-35 -4.969026707728702 2316 36-37 -8.264915559272485 2316 38-39 -5.097281482242458 2316 40-41 -4.255504476383894 2316 42-43 -3.232416004838356 2316 44-45 -2.4300036034511976 2316 46-47 -4.7347935826515375 2316 48-49 -2.294467667300516 2316 50-51 -3.1581693571360745 2316 52-53 -4.262047187572492 2316 54-55 -5.698256864099584 2316 56-57 -3.5603365708778156 2316 58-59 -4.4445814402881965 2316 60-61 -4.767340931417074 2316 62-63 -5.074580282742701 2316 64-65 -3.665056167679186 2316 66-67 -5.195404462426534 2316 68-69 -3.8630350139374947 2316 70-71 -4.69621786754238 2316 72-73 -7.150756899273876 2316 74-75 -6.5809172655043575 2316 76-77 -5.765817264451126 2316 78-79 -4.836474109121461 2316 80-81 -4.978072040277301 2316 82-83 -3.868970619654295 2316 84-85 -5.752250650631151 2316 86-87 -4.07170561652401 2316 88-89 -4.377076198874462 2316 90-91 -4.975594250296677 2316 92-93 -3.579147686519619 2316 94-95 -3.289684614605438 2316 96-97 -4.542586547232268 2316 98-99 -5.881078247278861 2316 100-101 -4.8272813708757205 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8505.0 3 2613.0 4 2609.0 5 3015.0 6 3322.0 7 3815.0 8 4510.0 9 5445.0 10 6563.0 11 8068.0 12 10026.0 13 12109.0 14 13622.0 15 16416.0 16 19705.0 17 23085.0 18 20719.0 19 18518.0 20 19730.0 21 21923.0 22 24344.0 23 27586.0 24 32072.0 25 36871.0 26 43850.0 27 52024.0 28 62849.0 29 75419.0 30 91413.0 31 112976.0 32 140473.0 33 175417.0 34 221738.0 35 285337.0 36 347363.0 37 315770.0 38 103530.0 39 1901.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.38340982216925 19.519630730185053 14.204973227854154 23.891986219791544 2 14.9570683257869 20.482398814143647 41.137763103451476 23.422769756617985 3 18.7649021508815 25.11335009410554 29.840410314489894 26.28133744052306 4 12.459329296810811 17.10811391589033 38.48815798898594 31.944398798312918 5 13.569280395307743 37.391012714867266 35.031865114041125 14.007841775783861 6 30.946125846786426 37.194735600417914 18.121566512756562 13.737572040039096 7 27.325823173185213 32.66148620948704 22.138385873135118 17.87430474419263 8 25.163756539412585 35.49637601627643 20.622474976753672 18.71739246755731 9 25.99103890933373 16.53201156894921 20.723670213326198 36.75327930839086 10-11 24.11249222648607 26.637599201834973 28.62688144316866 20.623027128510298 12-13 25.173031464920037 24.164135051229533 28.569324973472547 22.093508510377884 14-15 22.455745617222895 25.38027958341629 26.623415611461006 25.54055918789982 16-17 21.855425641215785 28.39498500359084 27.27629522438576 22.47329413080762 18-19 21.728061887140658 27.385944565756663 29.21868959625677 21.667303950845902 20-21 22.564732583050024 26.713841698874237 29.22660112222247 21.49482459585327 22-23 22.075330643013427 26.60744928679552 29.046563099464294 22.27065697072676 24-25 21.88405366439542 26.75160712257251 29.24352962325797 22.120809589774098 26-27 21.82438659826515 26.96862139017845 29.076096864541682 22.13089514701472 28-29 21.763391492857604 27.091957748812064 29.206456124495915 21.938194633834417 30-31 22.013430400454563 26.94752819848768 29.078469333947066 21.960572067110693 32-33 21.84356683581007 26.91812179378696 29.051933564726173 22.186377805676802 34-35 22.07897115498702 27.054009347427183 28.993378150573367 21.873641347012434 36-37 22.05476683388554 26.83442507174577 28.97273038996358 22.138077704405106 38-39 21.770260211767145 27.22767707744143 28.966082021203686 22.035980689587735 40-41 22.08009069893391 27.001814400141612 28.964590748455866 21.953504152468618 42-43 21.903684780270066 26.96572435131924 28.861017916361376 22.269572952049327 44-45 21.901494635646213 27.21970208429735 28.808000047199904 22.07080323285653 46-47 22.102140315220097 27.154466952806345 28.42241162385179 22.320981108121767 48-49 21.975255192160308 27.266103011044063 28.50005522453622 22.25858657225941 50-51 21.941098065685452 27.42866502341663 28.346020985903646 22.28421592499427 52-53 22.01535509851123 27.47761127639915 28.190049226626947 22.316984398462676 54-55 22.13118261753479 27.36494099676562 28.164924469662328 22.338951916037256 56-57 21.926151728248342 27.691847926538248 28.069454071758443 22.312546273454963 58-59 21.947076691717914 27.485866788501912 28.24956387562521 22.317492644154964 60-61 21.997171577280824 27.508528783974214 28.24117498184031 22.253124656904653 62-63 21.90084986969901 27.398314758391596 28.488283025511684 22.21255234639771 64-65 21.8226681839836 27.465515348717062 28.32620150831304 22.3856149589863 66-67 21.857192527736512 27.642570408066504 28.057864736437292 22.442372327759692 68-69 21.898231811546072 27.502777281416574 28.24788100592374 22.35110990111361 70-71 21.89987204148621 27.241929256980818 28.199784712750837 22.658413988782137 72-73 21.884971499612366 27.36009810827913 28.08914396816624 22.665786423942265 74-75 21.77082946852897 27.680881276582824 27.883952151754425 22.664337103133782 76-77 21.86386630586295 27.488761425146578 27.997564659225162 22.64980760976531 78-79 21.844997695839904 27.570740666254324 27.94450789844156 22.639753739464215 80-81 21.831618689095134 27.554077808965904 27.775086238193243 22.83921726374572 82-83 21.893490500523473 27.59747720686423 27.931336614021333 22.57769567859096 84-85 21.951073509037474 27.523717403259194 27.744002751933323 22.78120633577001 86-87 21.683460591976644 27.479330350791216 28.03676009506194 22.800448962170194 88-89 21.932264608318985 27.446111525035093 27.861841592065478 22.75978227458044 90-91 21.547181223194464 27.55489205822822 28.11048665545221 22.787440063125104 92-93 21.34439920805627 27.39570281830954 28.453264799252683 22.806633174381513 94-95 21.680748305386143 27.36322026491063 27.86558027546 23.090451154243222 96-97 21.838234673998297 27.355026017727475 27.77445118197229 23.032288126301943 98-99 21.717556833374523 27.353847698112315 27.67792298277475 23.25067248573841 100-101 21.741771165246064 27.3208469999922 27.79149393760264 23.1458878971591 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2174.0 1 1357.0 2 558.5 3 602.0 4 571.5 5 574.5 6 809.5 7 1532.0 8 2537.0 9 2945.5 10 2528.5 11 2113.5 12 2066.0 13 2159.5 14 2358.5 15 2515.5 16 2624.5 17 2907.5 18 3259.5 19 3584.0 20 4076.0 21 4762.0 22 5723.0 23 7100.0 24 8834.5 25 11182.5 26 14792.0 27 19348.5 28 23188.5 29 26939.5 30 31013.0 31 35286.0 32 40456.0 33 45792.5 34 51458.0 35 56398.0 36 61564.0 37 66811.0 38 69457.0 39 70770.5 40 73151.0 41 75866.0 42 77440.0 43 78795.5 44 80632.0 45 82879.5 46 84506.0 47 85616.0 48 87162.5 49 87238.0 50 84585.5 51 82417.5 52 81450.0 53 79661.0 54 76576.5 55 71117.0 56 65279.0 57 59685.5 58 54130.5 59 48328.5 60 40580.0 61 32505.5 62 25171.0 63 18704.0 64 13697.0 65 10110.5 66 7337.0 67 5264.5 68 3766.0 69 2735.0 70 1919.0 71 1181.5 72 701.0 73 446.0 74 302.0 75 181.5 76 122.0 77 84.0 78 51.0 79 44.5 80 44.0 81 33.0 82 19.0 83 18.0 84 14.5 85 8.0 86 7.0 87 6.0 88 4.0 89 3.5 90 3.0 91 3.5 92 3.0 93 2.5 94 2.5 95 3.0 96 4.0 97 3.5 98 1.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07165558503080306 2 0.08188608277609398 3 0.07797070709579745 4 0.08087566324569488 5 0.07616037210383239 6 0.06614037842737462 7 0.07763390058566441 8 0.0740132306017343 9 0.06971894759753812 10-11 0.07607617047629914 12-13 0.07437108751875066 14-15 0.07992839493594572 16-17 0.07786545506138089 18-19 0.07992839493594572 20-21 0.08853801135122141 22-23 0.09678977084948075 24-25 0.1164298004716133 26-27 0.12152399893737548 28-29 0.12074513388269281 30-31 0.12091353713775935 32-33 0.11815593383604511 34-35 0.11478786873471478 36-37 0.11836643790487826 38-39 0.11135665241273449 40-41 0.10803068812517078 42-43 0.10657821005022206 44-45 0.09956842455807828 46-47 0.11577723785823055 48-49 0.13122823651058352 50-51 0.14000625618092571 52-53 0.14590037010825382 54-55 0.14270070826199 56-57 0.11811383302227849 58-59 0.11148295485403437 60-61 0.10737812551178802 62-63 0.10422056447929082 64-65 0.09272704232100103 66-67 0.10424161488617413 68-69 0.1021786750116093 70-71 0.10975682148960259 72-73 0.1337963861503479 74-75 0.14394268226810555 76-77 0.1477317555071022 78-79 0.1454162107499376 80-81 0.15248914746273132 82-83 0.15131032467726568 84-85 0.13053357308343413 86-87 0.1310808836624003 88-89 0.15324696211053063 90-91 0.2520786224276929 92-93 0.5563412035191229 94-95 0.14926843520958416 96-97 0.15960418498929166 98-99 0.17669711537854316 100-101 0.1837911024982202 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2375251.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.8157294267557 #Duplication Level Percentage of deduplicated Percentage of total 1 77.78604548120731 46.5282904968121 2 12.686225368180743 15.176716481398872 3 4.130121142364771 7.411386262542427 4 1.907550407181497 4.564060760954644 5 0.988526801031753 2.956472583080586 6 0.5755454070093323 2.0656001003089326 7 0.38064627811019186 1.593804434511859 8 0.2738439049278415 1.3104138337863982 9 0.18574058686156997 0.9999187818550634 >10 0.9896115285312818 10.691651761220426 >50 0.06802546363003645 2.7745103147371704 >100 0.02719604715260439 2.7953264587549316 >500 5.671284991697088E-4 0.23021134895757325 >1k 2.1267318718825164E-4 0.28118096425410943 >5k 1.4178212479216778E-4 0.6204554168249328 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8697 0.366150777328375 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5935 0.24986832970494485 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4342 0.1828017333747044 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.21008137666293E-5 2 0.0 0.0 0.0 0.0 4.21008137666293E-5 3 0.0 0.0 0.0 0.0 4.21008137666293E-5 4 0.0 0.0 0.0 4.21008137666293E-5 4.21008137666293E-5 5 0.0 0.0 0.0 4.21008137666293E-5 4.21008137666293E-5 6 0.0 0.0 0.0 8.42016275332586E-5 4.21008137666293E-5 7 0.0 0.0 0.0 8.42016275332586E-5 4.21008137666293E-5 8 0.0 0.0 0.0 8.42016275332586E-5 4.21008137666293E-5 9 0.0 0.0 0.0 8.42016275332586E-5 4.21008137666293E-5 10-11 0.0 0.0 0.0 8.42016275332586E-5 4.21008137666293E-5 12-13 2.105040688331465E-5 0.0 0.0 8.42016275332586E-5 2.526048825997758E-4 14-15 8.42016275332586E-5 0.0 0.0 1.263024412998879E-4 3.368065101330344E-4 16-17 1.0525203441657324E-4 0.0 0.0 1.263024412998879E-4 4.420585445496076E-4 18-19 1.263024412998879E-4 0.0 0.0 1.263024412998879E-4 5.473105789661808E-4 20-21 1.263024412998879E-4 0.0 0.0 1.263024412998879E-4 6.736130202660688E-4 22-23 1.263024412998879E-4 0.0 0.0 1.684032550665172E-4 7.78865054682642E-4 24-25 1.263024412998879E-4 0.0 0.0 2.526048825997758E-4 7.999154615659566E-4 26-27 1.4735284818320255E-4 0.0 0.0 4.631089514329223E-4 9.262179028658445E-4 28-29 2.105040688331465E-4 0.0 0.0 0.0011577723785823055 9.262179028658445E-4 30-31 2.526048825997758E-4 0.0 0.0 0.0029681073705473656 9.472683097491591E-4 32-33 2.526048825997758E-4 0.0 0.0 0.006988735085260463 9.683187166324738E-4 34-35 2.9470569636640506E-4 0.0 0.0 0.014019570984287555 0.0010104195303991031 36-37 2.9470569636640506E-4 0.0 0.0 0.025092085004911058 0.0010104195303991031 38-39 3.57856917016349E-4 0.0 0.0 0.04191136010467947 0.0010314699372824176 40-41 3.7890732389966365E-4 0.0 0.0 0.060372566941346406 0.0010946211579323617 42-43 3.7890732389966365E-4 0.0 0.0 0.07898112662619655 0.0010946211579323617 44-45 3.7890732389966365E-4 0.0 0.0 0.10457842139630717 0.0011156715648156764 46-47 4.420585445496076E-4 0.0 0.0 0.13699604799661172 0.0011577723785823058 48-49 5.052097651995516E-4 0.0 0.0 0.17240283237434695 0.0012209235992322496 50-51 5.052097651995516E-4 0.0 0.0 0.21086192575016283 0.0012209235992322496 52-53 5.052097651995516E-4 0.0 0.0 0.25113135411794374 0.0013472260405321375 54-55 5.052097651995516E-4 0.0 0.0 0.2978422069920189 0.001431427668065396 56-57 5.473105789661808E-4 0.0 0.0 0.3536889364534527 0.0014735284818320252 58-59 5.473105789661808E-4 0.0 0.0 0.4184399880265286 0.0015366797024819693 60-61 5.473105789661808E-4 0.0 0.0 0.49083233729824766 0.0015998309231319131 62-63 5.473105789661808E-4 0.0 0.0 0.5686556915458618 0.001684032550665172 64-65 5.473105789661808E-4 0.0 0.0 0.6520784540244378 0.001684032550665172 66-67 5.473105789661808E-4 0.0 0.0 0.7463000752341542 0.001684032550665172 68-69 5.473105789661808E-4 0.0 0.0 0.8525625291811265 0.001684032550665172 70-71 5.473105789661808E-4 0.0 0.0 0.9762126192137168 0.001726133364431801 72-73 5.473105789661808E-4 0.0 0.0 1.10788291426885 0.0019155870263816328 74-75 5.683609858494955E-4 0.0 0.0 1.2423950142532305 0.0020208390607982063 76-77 5.894113927328101E-4 0.0 0.0 1.396294538977144 0.0020208390607982063 78-79 5.894113927328101E-4 0.0 0.0 1.5715602266876216 0.0020208390607982063 80-81 6.315122064994394E-4 0.0 0.0 1.7688656904049298 0.002041889467681521 82-83 6.315122064994394E-4 0.0 0.0 1.9693497655616186 0.0021260910952147793 84-85 6.525626133827541E-4 0.0 0.0 2.17054955455234 0.0021892423158647234 86-87 6.736130202660688E-4 0.0 0.0 2.3987780659812374 0.0022313431296313527 88-89 6.736130202660688E-4 0.0 0.0 2.650330428236847 0.0022944943502812964 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3925 0.0 51.87704 1 GTATCAA 7050 0.0 41.404118 1 TCAACGC 8750 0.0 33.57545 4 ATCAACG 8775 0.0 33.26555 3 CAACGCA 9000 0.0 32.590065 5 TATCAAC 8975 0.0 32.57714 2 AACGCAG 9385 0.0 31.29578 6 ACGCAGA 10650 0.0 27.132957 7 CGCAGAG 10510 0.0 27.044056 8 TACATGG 6830 0.0 23.766878 2 GTACATG 7040 0.0 23.664211 1 GCAGAGT 11470 0.0 23.415356 9 ACATGGG 6830 0.0 22.307508 3 GAGTACT 6970 0.0 21.513506 12-13 AGAGTAC 9770 0.0 21.030088 10-11 CATGGGG 3950 0.0 20.786829 4 CAGAGTA 10695 0.0 20.298223 10-11 GTACTTT 7585 0.0 19.332668 14-15 AGTACTT 7545 0.0 19.307943 12-13 TACCGTA 150 7.780088E-4 18.979704 7 >>END_MODULE