FastQCFastQC Report
Fri 27 May 2016
SRR939573_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939573_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375251
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99440.4186504920953617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79580.33503827595483593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60670.25542563712213995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44550.051.2075271
GTATCAA78000.039.921161
TATCAAC100750.030.8522132
TCAACGC100350.030.6901454
ATCAACG101550.030.3274823
CAACGCA102600.030.0171155
AACGCAG107250.028.7736456
ACGCAGA122500.024.9999037
CGCAGAG122700.024.6534258
GCAGAGT133500.021.6386349
GAGTACT85050.020.18594212-13
AGAGTAC114900.019.9496710-11
AGTACTT88350.019.43196912-13
CAGAGTA123900.019.08816110-11
GTACTTT91450.018.5676114-15
GTACATG66650.018.5607451
TACATGG64750.018.0111682
TGGTATC24050.017.9888212
ACATGGG64600.017.1044983
TACGTCG1702.4079418E-715.75661966-67
Produced by FastQC (version 0.11.5)