##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939573_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2375251 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.96942133694502 31.0 31.0 33.0 28.0 34.0 2 31.211786880628615 31.0 31.0 34.0 28.0 34.0 3 31.222086002700348 31.0 31.0 34.0 28.0 34.0 4 35.10008963263251 35.0 35.0 37.0 32.0 37.0 5 34.79446466920759 35.0 35.0 37.0 32.0 37.0 6 34.841218043903574 35.0 35.0 37.0 32.0 37.0 7 34.768063248894535 35.0 35.0 37.0 32.0 37.0 8 34.72386707762674 35.0 35.0 37.0 31.0 37.0 9 36.2621003001367 38.0 35.0 39.0 32.0 39.0 10-11 36.10027698125377 37.5 35.0 39.0 31.0 39.0 12-13 36.036597395391055 37.0 35.0 39.0 30.0 39.0 14-15 36.92843619474321 38.0 36.0 40.0 31.0 41.0 16-17 36.84162305373201 38.0 36.0 40.0 31.0 41.0 18-19 36.796243639093305 38.5 36.0 40.0 31.0 41.0 20-21 36.711658894154766 38.0 35.0 40.0 30.0 41.0 22-23 36.52691631326542 38.0 35.0 40.0 30.0 41.0 24-25 36.35758199870246 38.0 35.0 40.0 30.0 41.0 26-27 36.00166087710309 38.0 34.0 40.0 29.0 41.0 28-29 35.73356478957382 38.0 34.0 40.0 27.5 41.0 30-31 35.423654805323736 38.0 34.0 40.0 27.0 41.0 32-33 35.18348355605366 38.0 34.0 40.0 27.0 41.0 34-35 35.12836053958087 38.0 33.5 40.0 27.0 41.0 36-37 34.99082833772094 38.0 33.0 40.0 26.0 41.0 38-39 34.99070961342612 38.0 33.0 40.0 26.0 41.0 40-41 34.83179482926225 38.0 33.0 40.0 26.0 41.0 42-43 34.564488131991105 37.5 33.0 40.0 25.0 41.0 44-45 34.2720139892584 37.0 33.0 40.0 24.0 41.0 46-47 33.89523991359229 37.0 32.5 40.0 24.0 41.0 48-49 33.494731083157106 37.0 32.0 39.5 23.0 40.5 50-51 33.14235506058097 37.0 31.0 39.0 21.5 40.0 52-53 32.79571590539274 36.5 31.0 39.0 19.5 40.0 54-55 32.364579785462674 36.0 31.0 39.0 18.0 40.0 56-57 29.932181483135885 35.0 26.5 38.5 5.5 40.0 58-59 24.77059077124902 29.5 11.5 36.5 2.0 39.5 60-61 25.276018408159814 29.5 16.0 36.0 2.0 39.0 62-63 27.89516897372109 32.5 24.5 37.0 2.0 39.0 64-65 27.95342176468929 33.5 25.0 37.0 2.0 39.0 66-67 21.433494818021337 22.5 7.5 33.0 2.0 37.5 68-69 14.689765839483911 11.5 2.0 25.5 2.0 33.5 70-71 13.512819066279732 10.0 2.0 22.0 2.0 31.0 72-73 14.212481965063901 11.5 2.0 25.0 2.0 32.0 74-75 14.909892680815627 10.5 2.0 25.5 2.0 32.5 76-77 16.420329262044305 15.5 2.0 27.5 2.0 33.0 78-79 19.050344153102138 22.5 2.0 31.5 2.0 34.5 80-81 22.013175660172337 28.0 2.0 33.5 2.0 35.0 82-83 23.124561783154704 30.5 2.0 34.0 2.0 35.0 84-85 23.15989341757987 31.0 2.0 34.0 2.0 35.0 86-87 22.852821870193928 31.0 2.0 34.0 2.0 35.0 88-89 22.28181274315851 30.0 2.0 34.0 2.0 35.0 90-91 20.84385755442267 29.5 2.0 34.0 2.0 35.0 92-93 18.025279012618036 20.5 2.0 33.5 2.0 34.5 94-95 11.830733467747198 2.0 2.0 28.0 2.0 34.0 96-97 9.796506348171203 2.0 2.0 21.0 2.0 32.5 98-99 9.310234160516089 2.0 2.0 19.0 2.0 32.0 100-101 8.988494058101649 2.0 2.0 10.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37562382939785266 1101 2 0.3531359919868109 1101 3 0.46581707354298985 1101 4 0.39803694834348136 1101 5 0.4515444911966 1101 6 0.42423647668336883 1101 7 0.3197798908394205 1101 8 0.29733239952109614 1101 9 0.2801895598034747 1101 10-11 0.3091436415628621 1101 12-13 0.2947048489956714 1101 14-15 0.5245724856558667 1101 16-17 0.5767167373841602 1101 18-19 0.4319400531991775 1101 20-21 0.4661185061384785 1101 22-23 0.6362976131115587 1101 24-25 0.4571358563081631 1101 26-27 0.3589783530891637 1101 28-29 0.4521142654050152 1101 30-31 0.2955808341124424 1101 32-33 0.3716103481545261 1101 34-35 0.34273046538761776 1101 36-37 0.18539460278434206 1101 38-39 0.3429493834972632 1101 40-41 0.5618201498826139 1101 42-43 0.5718656712371839 1101 44-45 0.31038820645900955 1101 46-47 0.7387359220485763 1101 48-49 0.609028038800453 1101 50-51 0.08427057746351352 1101 52-53 0.036077880447876964 1101 54-55 -0.13268020082514198 1101 56-57 -3.278834391508358 1101 58-59 -9.774929437353858 1101 60-61 -10.178663006490694 1101 62-63 -5.6583035964827495 1101 64-65 -7.399028896182532 1101 66-67 -9.637383880034392 1101 68-69 -5.312341947526205 1101 70-71 -4.880868093607694 1101 72-73 -5.260982870094324 1101 74-75 -6.20906266998694 1101 76-77 -7.672803987054177 1101 78-79 -10.294050656177145 1101 80-81 -8.89594176608275 1101 82-83 -6.20791392814068 1101 84-85 -5.481761426419478 1101 86-87 -5.298644754114466 1101 88-89 -5.546616558120455 1101 90-91 -10.381424949460751 1101 92-93 -13.685369308184896 1101 94-95 -8.613141062686001 1101 96-97 -7.0516037797892 1101 98-99 -6.796825321819465 1101 100-101 -6.559577545733183 1102 1 0.2697359528332832 1102 2 0.2873712004319309 1102 3 0.42472650093488085 1102 4 0.3042788038171551 1102 5 0.25088854884067757 1102 6 0.2736588312864612 1102 7 0.45514657247193924 1102 8 0.5093835608347277 1102 9 0.49747183402899253 1102 10-11 0.36506573882629567 1102 12-13 0.34224164309109284 1102 14-15 0.5224714716281156 1102 16-17 0.4440335060741418 1102 18-19 0.4392605451011917 1102 20-21 0.37077717519968445 1102 22-23 0.4339252973831762 1102 24-25 0.3515782779290717 1102 26-27 0.2973018399867371 1102 28-29 0.4356226262061682 1102 30-31 0.44335867881069646 1102 32-33 0.42508949816711805 1102 34-35 0.3104810156564568 1102 36-37 0.24886770257737112 1102 38-39 0.12233989916212806 1102 40-41 0.2731188664695168 1102 42-43 0.20096448966489078 1102 44-45 0.37620870931164774 1102 46-47 0.41269711851371227 1102 48-49 0.4211195805387504 1102 50-51 0.3048257193288393 1102 52-53 0.007098423817822663 1102 54-55 -0.1771802501029427 1102 56-57 -3.9036549381634877 1102 58-59 -10.298423726603632 1102 60-61 -10.526232241808646 1102 62-63 -5.787629229702556 1102 64-65 -7.026053940258791 1102 66-67 -7.827388835191588 1102 68-69 -4.824011890254443 1102 70-71 -4.294047169156045 1102 72-73 -4.877847925559232 1102 74-75 -5.283986426269859 1102 76-77 -7.429788021044914 1102 78-79 -9.574952494786855 1102 80-81 -6.88763092715547 1102 82-83 -5.139512910827744 1102 84-85 -4.596554760774868 1102 86-87 -4.43238129853567 1102 88-89 -4.601161751344215 1102 90-91 -9.316452161760491 1102 92-93 -11.839549571875914 1102 94-95 -8.205395002172853 1102 96-97 -7.007851931871461 1102 98-99 -6.802768499033408 1102 100-101 -6.459798611447876 1103 1 0.22002749656650877 1103 2 0.17082913221969065 1103 3 0.2656035630531761 1103 4 0.31136443209306464 1103 5 0.3595186429472008 1103 6 0.3403632382422188 1103 7 0.24885781930009188 1103 8 0.29131817245385605 1103 9 0.49037221964594124 1103 10-11 0.49576424497685423 1103 12-13 0.41976705118571545 1103 14-15 0.707257833841652 1103 16-17 0.46713056299759614 1103 18-19 0.4927607349126433 1103 20-21 0.3264194039191537 1103 22-23 0.5617270975949609 1103 24-25 0.5839543676799153 1103 26-27 0.6793197854942079 1103 28-29 0.5669055518333721 1103 30-31 0.6306181677183815 1103 32-33 0.6501240471817695 1103 34-35 0.7339794239042661 1103 36-37 0.7027048909702387 1103 38-39 0.7067907613057329 1103 40-41 0.7512475086849193 1103 42-43 0.6748742393135814 1103 44-45 0.6966618092323955 1103 46-47 0.7035109933979911 1103 48-49 0.5322274633751363 1103 50-51 0.47940480410462527 1103 52-53 0.6805336448316126 1103 54-55 0.7229369667949399 1103 56-57 -2.8424932754151477 1103 58-59 -9.570468661967837 1103 60-61 -9.911763114475132 1103 62-63 -5.3284835643967625 1103 64-65 -6.6622066951888925 1103 66-67 -7.570556039102371 1103 68-69 -4.352086919059163 1103 70-71 -4.097731509378091 1103 72-73 -4.802412857726614 1103 74-75 -5.263219804447537 1103 76-77 -6.835230115231354 1103 78-79 -9.121323599489518 1103 80-81 -6.465429064339201 1103 82-83 -4.512165659097796 1103 84-85 -3.989228454905369 1103 86-87 -3.740231750420133 1103 88-89 -3.7564765553056105 1103 90-91 -6.531157221401248 1103 92-93 -10.317298591183402 1103 94-95 -7.361613741558397 1103 96-97 -6.298384169848404 1103 98-99 -6.332066229954027 1103 100-101 -6.217371672207521 1104 1 0.09525949524361721 1104 2 0.19288794359018624 1104 3 0.05175862740096804 1104 4 0.11538538209354243 1104 5 0.2689235649889028 1104 6 0.26981045460789943 1104 7 0.24633644158633672 1104 8 0.27063122359182756 1104 9 0.09410620424826988 1104 10-11 0.22679784829425387 1104 12-13 0.28560684791856517 1104 14-15 0.3929036490885949 1104 16-17 0.26234049230565404 1104 18-19 0.3632959012120196 1104 20-21 0.218129716420151 1104 22-23 0.3326415135993912 1104 24-25 0.3343222010975211 1104 26-27 0.4359398648040127 1104 28-29 0.511216710856857 1104 30-31 0.3885184612346251 1104 32-33 0.5740691854015196 1104 34-35 0.5866118162683875 1104 36-37 0.6893946446137491 1104 38-39 0.5427355306426662 1104 40-41 0.3359171836546224 1104 42-43 0.49053528687719705 1104 44-45 0.5534630863301757 1104 46-47 0.41640058613226927 1104 48-49 0.35490548908881436 1104 50-51 0.2755455901430537 1104 52-53 0.17377443945609627 1104 54-55 0.08098055220879985 1104 56-57 -3.7313079213353397 1104 58-59 -10.089850719634399 1104 60-61 -10.068187876688809 1104 62-63 -5.339042199275905 1104 64-65 -6.635409711123998 1104 66-67 -7.596371922052034 1104 68-69 -4.436213573069333 1104 70-71 -3.9234573340379075 1104 72-73 -4.642848775465742 1104 74-75 -5.1056637167679035 1104 76-77 -6.794743825906377 1104 78-79 -8.917802231315836 1104 80-81 -6.140407996677823 1104 82-83 -4.247954607241137 1104 84-85 -3.630458806329859 1104 86-87 -3.6012717455864944 1104 88-89 -3.4469168932785976 1104 90-91 -6.012408306584561 1104 92-93 -7.682987018756757 1104 94-95 -6.836224935880146 1104 96-97 -6.160008144688052 1104 98-99 -6.1352374774457825 1104 100-101 -5.929515167626696 1105 1 0.2908956968162144 1105 2 0.35144713882782597 1105 3 0.3152425353371733 1105 4 0.37541021257774077 1105 5 0.32292668471640695 1105 6 0.3144015474675399 1105 7 0.28809876928506384 1105 8 0.1991028754557007 1105 9 0.5547689871725368 1105 10-11 0.3910874736086143 1105 12-13 0.4981612043951813 1105 14-15 0.5630516769751424 1105 16-17 0.5374097311558117 1105 18-19 0.5213260161325977 1105 20-21 0.4783993500113368 1105 22-23 0.5476620464997879 1105 24-25 0.5965887786291191 1105 26-27 0.5374370156651196 1105 28-29 0.6299455810698262 1105 30-31 0.6942804069258983 1105 32-33 0.7352760106012823 1105 34-35 0.6020229517855356 1105 36-37 0.6027212928167245 1105 38-39 0.6138685010689215 1105 40-41 0.5339870910178206 1105 42-43 0.7051464351279293 1105 44-45 0.8600367978508814 1105 46-47 0.9535890113065619 1105 48-49 0.8138699305620847 1105 50-51 0.713237946810672 1105 52-53 0.8244579144505266 1105 54-55 0.8058552855994421 1105 56-57 1.8251661499090481 1105 58-59 -7.36386975633895 1105 60-61 -9.009626134734841 1105 62-63 -4.124739203700827 1105 64-65 -5.534854526964573 1105 66-67 -6.524774494235524 1105 68-69 -3.530193135670805 1105 70-71 -3.4959139602754536 1105 72-73 -2.365990468395422 1105 74-75 -4.299239943170987 1105 76-77 -6.086923496743292 1105 78-79 -8.363276716401629 1105 80-81 -5.3464373475835 1105 82-83 -3.194427405127364 1105 84-85 -2.6242457735090525 1105 86-87 -2.380268657692948 1105 88-89 -2.4310495771992677 1105 90-91 -5.201371156360132 1105 92-93 -7.058698348461018 1105 94-95 -6.882550979237992 1105 96-97 -6.517090881134746 1105 98-99 -6.492809501766923 1105 100-101 -6.329057395366804 1106 1 0.30645392701401875 1106 2 0.27294716865437607 1106 3 0.30412074125265676 1106 4 0.23546053172182013 1106 5 0.24205041577216235 1106 6 0.2794185135108904 1106 7 0.10445143841037208 1106 8 0.06658316361526317 1106 9 0.27453240999721373 1106 10-11 0.3097032760656191 1106 12-13 0.3249501833095607 1106 14-15 0.35485727103229436 1106 16-17 0.3760096729063136 1106 18-19 0.38067067419751766 1106 20-21 0.3736818425283843 1106 22-23 0.31146998457284525 1106 24-25 0.46191839709490523 1106 26-27 0.5297621180403667 1106 28-29 0.43165043156862737 1106 30-31 0.46145699062797263 1106 32-33 0.2809857245822158 1106 34-35 0.5312729719623164 1106 36-37 0.47414079095869965 1106 38-39 0.519944169271362 1106 40-41 0.39115511274558656 1106 42-43 0.22104889880093737 1106 44-45 0.2638824928270367 1106 46-47 0.6145218454253296 1106 48-49 0.776842023438455 1106 50-51 0.8382169805006399 1106 52-53 0.8339355638376844 1106 54-55 0.4373679127570327 1106 56-57 1.4492124898404875 1106 58-59 -8.117018562750602 1106 60-61 -9.180626482126423 1106 62-63 -4.301742933774058 1106 64-65 -6.3186060753134115 1106 66-67 -7.781894793483545 1106 68-69 -4.423378821734085 1106 70-71 -4.441465242641646 1106 72-73 -4.915335512450991 1106 74-75 -5.396685036001738 1106 76-77 -6.782990602971427 1106 78-79 -9.254421213754085 1106 80-81 -6.495811165487151 1106 82-83 -4.342405165048202 1106 84-85 -3.815708186792744 1106 86-87 -3.4595039400451064 1106 88-89 -3.3040788317811547 1106 90-91 -5.5922714269044835 1106 92-93 -7.509224348439965 1106 94-95 -6.964344853215556 1106 96-97 -6.228929389666893 1106 98-99 -5.928215370930131 1106 100-101 -5.722472394963268 1107 1 0.039538024605388244 1107 2 -0.0197782631270762 1107 3 -0.010734098399193925 1107 4 0.04874791221522656 1107 5 -0.098020071211117 1107 6 -0.07143920792922387 1107 7 0.03141368308977377 1107 8 0.035798655174353655 1107 9 -0.021211110847069392 1107 10-11 0.07455519498611807 1107 12-13 3.206294026014689E-4 1107 14-15 0.0769156077731381 1107 16-17 0.0962717598662266 1107 18-19 0.11406631646634224 1107 20-21 0.1486443598093743 1107 22-23 0.2039724671944967 1107 24-25 0.203599180629773 1107 26-27 0.15173672714914943 1107 28-29 0.23085015117841579 1107 30-31 0.13398945942482499 1107 32-33 0.19968716742350523 1107 34-35 0.08935171487954108 1107 36-37 0.2170142064619469 1107 38-39 0.03160275524795253 1107 40-41 0.08240946950318317 1107 42-43 0.02764780450026194 1107 44-45 0.028349848786305643 1107 46-47 0.19835657097394233 1107 48-49 -0.07650805542129291 1107 50-51 -0.12742548303889834 1107 52-53 -0.006266356056400468 1107 54-55 -0.10489020200823518 1107 56-57 0.8171321502567856 1107 58-59 -8.11582106289664 1107 60-61 -9.993870051649564 1107 62-63 -5.184193232662235 1107 64-65 -6.529531557720873 1107 66-67 -7.738575479660698 1107 68-69 -4.074589951667688 1107 70-71 -3.6757711795149053 1107 72-73 -2.771114666217761 1107 74-75 -4.770903740365309 1107 76-77 -6.721898638876951 1107 78-79 -7.547769734890444 1107 80-81 -3.8387731765080453 1107 82-83 -2.64813758711335 1107 84-85 -2.1905937914814935 1107 86-87 -2.114886847925238 1107 88-89 -1.9551341893760288 1107 90-91 -1.4876508299069968 1107 92-93 -4.173072509352792 1107 94-95 -5.620068513086654 1107 96-97 -5.5607098674985425 1107 98-99 -5.33058018974743 1107 100-101 -5.510894615887388 1108 1 -0.08009227270930808 1108 2 -0.18710893189802036 1108 3 -0.02561886569906946 1108 4 0.013534629606951398 1108 5 -0.006845846040626213 1108 6 -0.12941674947479953 1108 7 -0.12142484571251799 1108 8 -0.15629790206562433 1108 9 -0.18518349007032953 1108 10-11 -0.171094147748029 1108 12-13 -0.05151131070248738 1108 14-15 -0.1345681001172565 1108 16-17 -0.0538288562336362 1108 18-19 -0.08060348950818508 1108 20-21 -0.22418138080424654 1108 22-23 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2312 16-17 -0.4222469136791318 2312 18-19 -0.29370476757903674 2312 20-21 -0.15043388489454657 2312 22-23 -0.48233611587605196 2312 24-25 -0.23327598064913246 2312 26-27 -0.20977204003452954 2312 28-29 -0.1732518148343729 2312 30-31 -0.13231169802347864 2312 32-33 -0.2722343312950031 2312 34-35 -0.38738623100476843 2312 36-37 -0.2857065145048807 2312 38-39 -0.2519334535459663 2312 40-41 -0.4970590080988515 2312 42-43 -0.5485638122219001 2312 44-45 -0.44786877351696575 2312 46-47 -0.2511895128988755 2312 48-49 -0.1567193155490898 2312 50-51 -0.34852859917130985 2312 52-53 -0.17827481503857 2312 54-55 -0.16863687784530867 2312 56-57 1.728172138319394 2312 58-59 6.681009374282464 2312 60-61 5.958021580088335 2312 62-63 2.455665410278865 2312 64-65 1.7046298441120626 2312 66-67 3.1931592446843737 2312 68-69 4.502386257408228 2312 70-71 2.6562198580410286 2312 72-73 3.0320669103669733 2312 74-75 2.5623438223313357 2312 76-77 4.372355874031751 2312 78-79 6.404694827076419 2312 80-81 4.171188554220038 2312 82-83 2.95310723268533 2312 84-85 2.418527313573289 2312 86-87 2.341401630449326 2312 88-89 2.323835907954553 2312 90-91 3.692740240136036 2312 92-93 5.90373458176397 2312 94-95 10.493388488580438 2312 96-97 12.048161048721317 2312 98-99 11.780983102006239 2312 100-101 10.504916628202482 2313 1 -0.21554047711469337 2313 2 -0.3163153574096427 2313 3 -0.26470570936211857 2313 4 -0.3655507983339561 2313 5 -0.4520364745823571 2313 6 -0.3303249896777274 2313 7 -0.2440136258812089 2313 8 -0.3479050033901032 2313 9 -0.23019629768847238 2313 10-11 -0.29312737365761166 2313 12-13 -0.2587363164003804 2313 14-15 -0.34237464090910663 2313 16-17 -0.34186468178243956 2313 18-19 -0.36285095618231367 2313 20-21 -0.30872309521263475 2313 22-23 -0.24842734694463786 2313 24-25 -0.4326091711305793 2313 26-27 -0.16606481246106597 2313 28-29 -0.3347135229700271 2313 30-31 -0.305402185289978 2313 32-33 -0.23106285822870376 2313 34-35 -0.3628455700642519 2313 36-37 -0.35751250476413077 2313 38-39 -0.14468531773902527 2313 40-41 -0.1536139623480537 2313 42-43 -0.09055008594010872 2313 44-45 -0.2967396800211546 2313 46-47 -0.11150697423390454 2313 48-49 -0.06962251085299442 2313 50-51 -0.23258354019842642 2313 52-53 -0.27104445291224977 2313 54-55 -0.14470563458355912 2313 56-57 1.7829296589983237 2313 58-59 6.801320524278061 2313 60-61 5.8248758774165275 2313 62-63 2.650371033092515 2313 64-65 1.8419330494807724 2313 66-67 3.203631933236437 2313 68-69 4.585276677779012 2313 70-71 5.350276076401196 2313 72-73 4.412003614267553 2313 74-75 3.346984830042935 2313 76-77 4.611607725483527 2313 78-79 6.258312219832931 2313 80-81 3.7142607212932752 2313 82-83 2.6758892608498037 2313 84-85 2.4351763160755517 2313 86-87 2.5952031386639227 2313 88-89 2.7323098667878867 2313 90-91 3.7866149233470274 2313 92-93 6.094499624123507 2313 94-95 11.074283416638702 2313 96-97 12.252801742308352 2313 98-99 11.697858210086725 2313 100-101 10.464281891442868 2314 1 -0.27261814984332844 2314 2 -0.4290704206937619 2314 3 -0.46944358961520294 2314 4 -0.3905674206034462 2314 5 -0.3308059742755205 2314 6 -0.2611451098360362 2314 7 -0.3802776566556645 2314 8 -0.512575831243133 2314 9 -0.5904375361094267 2314 10-11 -0.6241939045579628 2314 12-13 -0.5819451014938508 2314 14-15 -0.8128834487915242 2314 16-17 -0.847944634258539 2314 18-19 -0.691382879048156 2314 20-21 -0.5767067691140042 2314 22-23 -0.6847586349635506 2314 24-25 -0.7239358032069916 2314 26-27 -0.6956929942707077 2314 28-29 -0.5684381660073257 2314 30-31 -0.6092949970439463 2314 32-33 -0.5617622908950679 2314 34-35 -0.5569902636127608 2314 36-37 -0.556389786752078 2314 38-39 -0.5195599572342147 2314 40-41 -0.5710058493149148 2314 42-43 -0.6644200490083705 2314 44-45 -0.6376764676000732 2314 46-47 -0.44408167202440296 2314 48-49 -0.445566864871914 2314 50-51 -0.6099584762912471 2314 52-53 -0.52699846875867 2314 54-55 -0.35728316605491983 2314 56-57 1.496315643032414 2314 58-59 6.643182440375504 2314 60-61 5.875523911209022 2314 62-63 2.6778844525344674 2314 64-65 1.8390344158782668 2314 66-67 2.979184522341619 2314 68-69 8.95707481045161 2314 70-71 6.322527085900001 2314 72-73 4.943784369306934 2314 74-75 5.093156160191505 2314 76-77 5.913990151199691 2314 78-79 6.776789696764464 2314 80-81 4.276204387376421 2314 82-83 3.019159652896377 2314 84-85 2.352866336995284 2314 86-87 2.3817581765131948 2314 88-89 2.7096977763593273 2314 90-91 3.768188230756163 2314 92-93 5.848382236333428 2314 94-95 11.113281949440692 2314 96-97 12.282741031549493 2314 98-99 12.004957178097712 2314 100-101 10.912221114109586 2315 1 -0.3150805773570262 2315 2 -0.29628134649368576 2315 3 -0.24829920437386122 2315 4 -0.3165442870061028 2315 5 -0.3556020823150021 2315 6 -0.46864829750055037 2315 7 -0.40476275078981416 2315 8 -0.36085102656169 2315 9 -0.6968482532290565 2315 10-11 -0.5146125785260054 2315 12-13 -0.3079351928279621 2315 14-15 -0.6723457504403498 2315 16-17 -0.6600086903541396 2315 18-19 -0.44806913077423616 2315 20-21 -0.371715132743077 2315 22-23 -0.4477075757833475 2315 24-25 -0.5381304413637551 2315 26-27 -0.4102032066539891 2315 28-29 -0.4503232933406096 2315 30-31 -0.46443064835814596 2315 32-33 -0.5268634296984729 2315 34-35 -0.460579145184866 2315 36-37 -0.4787870746378644 2315 38-39 -0.2899806195023018 2315 40-41 -0.35174296139118866 2315 42-43 -0.4322997915143958 2315 44-45 -0.37540072139471903 2315 46-47 -0.35800652157940505 2315 48-49 -0.2174398070269703 2315 50-51 -0.19745544367765433 2315 52-53 -0.27216598759736144 2315 54-55 0.023742796765695573 2315 56-57 1.9009967215301309 2315 58-59 6.870942757190139 2315 60-61 6.251170610357342 2315 62-63 3.019764709444722 2315 64-65 2.4723083434275352 2315 66-67 8.637786291381417 2315 68-69 14.327247938485362 2315 70-71 7.875447515591704 2315 72-73 11.392196191848422 2315 74-75 7.4474429533189195 2315 76-77 7.052961014395528 2315 78-79 7.702796296461294 2315 80-81 4.923552469396533 2315 82-83 3.5986508852228525 2315 84-85 3.065777153529911 2315 86-87 2.872467342941203 2315 88-89 2.886757445425939 2315 90-91 4.056950413654231 2315 92-93 6.139765509440117 2315 94-95 11.259542850888502 2315 96-97 12.379772809691696 2315 98-99 11.993964615069508 2315 100-101 10.724265195998361 2316 1 -0.4427776643177914 2316 2 -0.36504393748257513 2316 3 -0.4659593677304201 2316 4 -0.38090573581420273 2316 5 -0.41460014347706675 2316 6 -0.49766889541187 2316 7 -0.4769833678304636 2316 8 -0.43993449223948033 2316 9 -0.49281096696451954 2316 10-11 -0.4991788905693184 2316 12-13 -0.4810115487691249 2316 14-15 -3.0926016252388493 2316 16-17 -0.9020687206024007 2316 18-19 -0.7622122859589879 2316 20-21 -0.5862874027775717 2316 22-23 -0.4349834785983475 2316 24-25 -0.45852510348188247 2316 26-27 -0.5910979813562349 2316 28-29 -0.6831494335785777 2316 30-31 -0.5418770613087034 2316 32-33 -0.5585475884161681 2316 34-35 -0.5169510075253783 2316 36-37 -0.5318591264783592 2316 38-39 -0.58304105425632 2316 40-41 -0.4457169215822532 2316 42-43 -0.538402149404348 2316 44-45 -0.44173488300174313 2316 46-47 -0.3079611890378118 2316 48-49 -0.3344106168554717 2316 50-51 -0.27986751349175876 2316 52-53 -0.29880488351583523 2316 54-55 0.1277262817720839 2316 56-57 1.965910729185854 2316 58-59 6.886164926496718 2316 60-61 6.163576682327481 2316 62-63 3.0868342545451206 2316 64-65 2.380538939605252 2316 66-67 8.717013426344863 2316 68-69 14.836424839014805 2316 70-71 15.404649941829186 2316 72-73 14.2737752388488 2316 74-75 12.846031766243975 2316 76-77 10.912077637589451 2316 78-79 8.997483123322866 2316 80-81 5.647380947000265 2316 82-83 4.090159950734851 2316 84-85 3.6742604163570327 2316 86-87 3.573260014811318 2316 88-89 3.5166837974814484 2316 90-91 4.5985223469327 2316 92-93 6.740783449170756 2316 94-95 11.73998878361302 2316 96-97 12.56005843459331 2316 98-99 12.000110711389706 2316 100-101 10.907623171636354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 23.0 4 69.0 5 157.0 6 365.0 7 976.0 8 2136.0 9 3610.0 10 5624.0 11 8617.0 12 12469.0 13 17224.0 14 23011.0 15 32112.0 16 44135.0 17 57976.0 18 65796.0 19 70436.0 20 76149.0 21 76270.0 22 71113.0 23 62829.0 24 57153.0 25 56940.0 26 78640.0 27 117721.0 28 166004.0 29 205644.0 30 217187.0 31 206207.0 32 177816.0 33 144930.0 34 119569.0 35 95651.0 36 63492.0 37 30171.0 38 6815.0 39 209.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.49624949520938 22.26199254067907 16.341698715694765 21.900059248416778 2 16.998170211583496 22.950203314502147 38.06275677455373 21.988869699360627 3 19.860284062969424 26.153226947249298 31.085427711127426 22.90106127865385 4 14.604645993202404 19.481309554232375 36.97849195727104 28.935552495294182 5 13.22997522743307 38.36178230990911 35.03055058483993 13.37769187781789 6 30.545361311288787 38.91411897100559 17.22508484366494 13.31543487404068 7 26.62510193659533 33.482692986972744 22.127177296209958 17.765027780221963 8 25.181224860286978 36.704338871332666 20.06419599650728 18.05024027187308 9 25.75155215175154 16.459481545318788 20.510548148385162 37.278418154544504 10-11 24.371255922005716 27.35174093180047 28.321933134645562 19.955070011548255 12-13 25.286801268581723 24.709851716723833 28.537447200316933 21.46589981437751 14-15 22.371168981912724 25.990286849692882 26.670241052090248 24.968303116304146 16-17 21.615231400807748 28.938162745747714 27.40438799941564 22.0422178540289 18-19 21.54943853219507 27.722395245127085 29.621144743863535 21.107021478814314 20-21 22.403990146725548 26.967992014317645 29.651245278919998 20.976772560036814 22-23 22.05531521742105 26.901348768114737 29.45548905237636 21.587846962087855 24-25 21.926293263322485 27.03274306589072 29.622237818234787 21.418725852552004 26-27 21.89269618963758 27.188714939691522 29.475046063640132 21.443542807030767 28-29 21.888326638393686 27.25910532141394 29.544661303136564 21.307906737055806 30-31 22.103277528884696 27.136288611176607 29.45820140128676 21.302232458651936 32-33 22.02197846613016 27.081871607242963 29.37354235253328 21.522607574093595 34-35 22.159215662003593 27.21243441728615 29.386192321788585 21.24215759892167 36-37 22.227727903607878 26.973512897199097 29.41232609701404 21.386433102178987 38-39 21.978049528135433 27.291310929202933 29.327465267207337 21.403174275454298 40-41 22.220894838098115 27.1593958674529 29.302109887600064 21.317599406848924 42-43 22.07908120802569 27.14425318864624 29.172062372604803 21.604603230723267 44-45 22.174258765843707 27.310782549261454 29.05529174249917 21.459666942395675 46-47 22.273165687616693 27.330853157673186 28.67489262474302 21.721088529967105 48-49 22.249482350184937 27.45522339633899 28.635536607133076 21.659757646342996 50-51 22.25131755948901 27.6237157547775 28.43351841844404 21.691448267289445 52-53 22.36274325383543 27.645250842961183 28.258784606592485 21.733221296610903 54-55 22.480618358969455 27.59696718153753 28.227572176036926 21.694842283456094 56-57 22.621952278318734 27.196187110597425 28.990440671046176 21.19141994003767 58-59 24.01032337408357 25.35589904949422 30.717005176674515 19.916772399747696 60-61 23.07972885233901 26.83664096475423 29.14403380518073 20.939596377726026 62-63 22.27418114178919 27.60633252314469 28.526941474010037 21.592544861056084 64-65 22.07823825852781 27.55528734071875 28.634496062919094 21.73197833783434 66-67 25.158588751813678 24.355340187451873 31.247456017974073 19.238615042760376 68-69 26.655587275383503 22.450331025211224 32.95575572351413 17.938325975891146 70-71 26.517936215947856 22.49891872145723 32.81009930418777 18.173045758407145 72-73 25.935768975936146 23.084180949571174 32.399954371474635 18.58009570301804 74-75 25.373675467316097 24.17678714470805 31.04183130923773 19.407706078738126 76-77 22.4961655417141 26.03759105168369 30.62070660916315 20.845536797439063 78-79 22.46264827291126 26.472053696915594 29.948219980762985 21.11707804941016 80-81 22.028154964357658 27.62559491663795 28.104477008351793 22.2417731106526 82-83 22.36473833469179 27.726999454372763 27.85542814897644 22.052834061959004 84-85 22.250497632247242 27.687593042915942 27.826168046452416 22.235741278384406 86-87 22.14771995051457 27.646543334801954 27.934976799230565 22.270759915452913 88-89 22.325561030299752 27.633422243253925 27.801720281713443 22.23929644473288 90-91 22.097619495443393 27.420966315677926 28.448537729864693 22.032876459013984 92-93 21.641891244697213 27.667200002706505 28.24474224898934 22.446166503606943 94-95 23.243193372335366 24.635928567910785 31.927744657185197 20.193133402568648 96-97 23.722589347522526 23.89920547581275 32.678994733050885 19.699210443613836 98-99 22.625391611611928 25.696712707265 30.30752120913332 21.37037447198975 100-101 22.381678934794095 26.260179285134814 29.63421026609946 21.723931513971632 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 510.0 1 399.0 2 313.5 3 342.5 4 371.5 5 428.0 6 558.5 7 806.5 8 1125.5 9 1434.5 10 1601.5 11 1813.5 12 2087.5 13 2273.5 14 2628.5 15 3042.5 16 3359.5 17 3672.5 18 4041.0 19 4481.5 20 5077.5 21 5843.0 22 6904.0 23 8296.0 24 10164.0 25 12500.5 26 15564.0 27 19195.0 28 23200.5 29 27516.5 30 31869.5 31 37057.5 32 42658.0 33 47913.0 34 53604.5 35 59527.5 36 64996.0 37 69506.5 38 72986.0 39 76034.5 40 78981.5 41 81702.0 42 83835.5 43 85677.0 44 87455.0 45 88989.5 46 89712.5 47 90099.5 48 90202.5 49 89133.5 50 86907.5 51 84095.0 52 80982.5 53 76867.0 54 72100.0 55 66339.5 56 59708.0 57 53086.0 58 46166.0 59 38836.0 60 31232.5 61 24388.5 62 18611.0 63 13727.0 64 9816.5 65 6917.0 66 4909.0 67 3380.0 68 2226.5 69 1487.5 70 942.5 71 556.5 72 310.0 73 167.5 74 114.0 75 69.5 76 33.0 77 12.5 78 4.5 79 4.5 80 6.5 81 4.0 82 1.0 83 1.0 84 0.5 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021218810138381165 2 0.00475739195562911 3 1.263024412998879E-4 4 0.0 5 0.10377850593474121 6 0.0 7 0.0 8 0.0010525203441657325 9 0.0 10-11 0.0 12-13 0.0 14-15 7.367642409160127E-4 16-17 0.0 18-19 1.4735284818320253E-4 20-21 0.0 22-23 3.999577307829783E-4 24-25 0.0 26-27 1.8945366194983183E-4 28-29 0.0031154602187305675 30-31 0.012251336806089124 32-33 0.002631300860414331 34-35 0.004273232597312874 36-37 0.08188608277609398 38-39 0.08224393969311034 40-41 0.09104300977033584 42-43 0.09354800818945029 44-45 0.09043254797071973 46-47 0.08554885357379073 48-49 0.08525414787742432 50-51 0.08498049258794123 52-53 0.06839277196388929 54-55 0.05976210514173028 56-57 0.5937056757370063 58-59 1.6826853246246398 60-61 0.5626563255841173 62-63 0.003536468356396861 64-65 0.1342594951017808 66-67 1.9978309660747433 68-69 3.0133446949396085 70-71 2.902998462057273 72-73 2.748867382857643 74-75 2.110871651038143 76-77 1.5267228600261613 78-79 1.0127140247493844 80-81 0.0584780303218481 82-83 1.263024412998879E-4 84-85 1.263024412998879E-4 86-87 2.736552894830904E-4 88-89 2.105040688331465E-5 90-91 0.2802440668375679 92-93 0.4452371559889881 94-95 2.118912906467569 96-97 2.540510455526595 98-99 1.5374375171297685 100-101 1.0090933547654544 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2375251.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.14951081707708 #Duplication Level Percentage of deduplicated Percentage of total 1 77.66883994936906 45.940738887350335 2 12.751085790999001 15.084409738483465 3 4.182985520903092 7.422646419490027 4 1.84720818287358 4.3704584157709645 5 0.9789144171691286 2.895115445366904 6 0.5987434699371501 2.1249230011021036 7 0.3920046161320734 1.6230816897573757 8 0.27283175565468 1.2910291905870899 9 0.1899753595613519 1.0113254626817063 >10 1.0138522323461483 10.77925876656887 >50 0.07241822580016927 2.9241416180574786 >100 0.030073351959683227 3.0900730911709298 >500 6.402763768958317E-4 0.2620323985708668 >1k 2.1342545896475284E-4 0.17214943673541871 >5k 2.1342545896475284E-4 1.0086164383063434 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9944 0.4186504920953617 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7958 0.33503827595483593 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6067 0.25542563712213995 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.21008137666293E-5 2 0.0 0.0 0.0 0.0 4.21008137666293E-5 3 0.0 0.0 0.0 0.0 4.21008137666293E-5 4 0.0 0.0 0.0 0.0 4.21008137666293E-5 5 0.0 0.0 0.0 0.0 4.21008137666293E-5 6 0.0 0.0 0.0 0.0 4.21008137666293E-5 7 0.0 0.0 0.0 0.0 4.21008137666293E-5 8 0.0 0.0 0.0 0.0 4.21008137666293E-5 9 0.0 0.0 0.0 0.0 4.21008137666293E-5 10-11 0.0 0.0 0.0 0.0 6.315122064994395E-5 12-13 0.0 0.0 0.0 0.0 2.3155447571646115E-4 14-15 0.0 0.0 0.0 4.21008137666293E-5 2.947056963664051E-4 16-17 0.0 0.0 0.0 4.21008137666293E-5 3.7890732389966365E-4 18-19 2.105040688331465E-5 0.0 0.0 4.21008137666293E-5 4.841593583162369E-4 20-21 4.21008137666293E-5 0.0 0.0 4.21008137666293E-5 5.262601720828662E-4 22-23 4.21008137666293E-5 0.0 0.0 1.263024412998879E-4 6.104617996161247E-4 24-25 8.42016275332586E-5 0.0 0.0 2.105040688331465E-4 6.315122064994394E-4 26-27 8.42016275332586E-5 0.0 0.0 3.9995773078297834E-4 6.736130202660688E-4 28-29 1.263024412998879E-4 0.0 0.0 0.0010946211579323617 6.736130202660688E-4 30-31 1.263024412998879E-4 0.0 0.0 0.0029470569636640504 6.736130202660688E-4 32-33 1.263024412998879E-4 0.0 0.0 0.006988735085260463 6.946634271493835E-4 34-35 1.263024412998879E-4 0.0 0.0 0.013830117322337724 7.578146477993273E-4 36-37 1.263024412998879E-4 0.0 0.0 0.024776328901661343 7.578146477993273E-4 38-39 1.263024412998879E-4 0.0 0.0 0.04132194871194665 7.999154615659567E-4 40-41 1.263024412998879E-4 0.0 0.0 0.05936214741094731 8.42016275332586E-4 42-43 1.263024412998879E-4 0.0 0.0 0.07750759814436453 8.42016275332586E-4 44-45 1.263024412998879E-4 0.0 0.0 0.10266283436992554 8.630666822159005E-4 46-47 2.526048825997758E-4 0.0 0.0 0.13196500075149953 9.051674959825299E-4 48-49 2.526048825997758E-4 0.0 0.0 0.1567834304669275 9.262179028658445E-4 50-51 2.526048825997758E-4 0.0 0.0 0.18103349919650596 9.262179028658445E-4 52-53 2.526048825997758E-4 0.0 0.0 0.20762016309013237 9.683187166324738E-4 54-55 2.526048825997758E-4 0.0 0.0 0.23694337987858965 9.683187166324738E-4 56-57 2.526048825997758E-4 2.105040688331465E-5 0.0 0.260982944539335 0.0010314699372824176 58-59 2.526048825997758E-4 4.21008137666293E-5 0.0 0.2750656667442725 0.0010525203441657323 60-61 2.526048825997758E-4 6.315122064994395E-5 0.0 0.2879485157568611 0.0010525203441657323 62-63 4.631089514329223E-4 1.263024412998879E-4 0.0 0.2996314915771007 0.0010946211579323617 64-65 5.052097651995516E-4 1.8945366194983183E-4 4.21008137666293E-5 0.30988303972927495 0.0010946211579323617 66-67 5.052097651995516E-4 2.105040688331465E-4 4.21008137666293E-5 0.3197556805575495 0.0010946211579323617 68-69 5.052097651995516E-4 2.105040688331465E-4 4.21008137666293E-5 0.3343015117139199 0.0011156715648156764 70-71 5.052097651995516E-4 2.105040688331465E-4 4.21008137666293E-5 0.3589515381742814 0.001136721971698991 72-73 5.262601720828662E-4 2.105040688331465E-4 4.21008137666293E-5 0.3977474380602303 0.001136721971698991 74-75 5.473105789661808E-4 2.105040688331465E-4 4.21008137666293E-5 0.45066816096488327 0.001199873192348935 76-77 5.473105789661808E-4 2.105040688331465E-4 4.21008137666293E-5 0.5460054537394153 0.0012209235992322496 78-79 5.473105789661808E-4 2.105040688331465E-4 4.21008137666293E-5 0.6930635962262515 0.0012209235992322496 80-81 5.894113927328101E-4 2.105040688331465E-4 4.21008137666293E-5 0.8794649491780027 0.0012209235992322496 82-83 5.894113927328101E-4 2.526048825997758E-4 4.21008137666293E-5 1.063403404524406 0.001241974006115564 84-85 5.894113927328101E-4 2.526048825997758E-4 4.21008137666293E-5 1.1976207988124203 0.0012630244129988788 86-87 5.894113927328101E-4 2.526048825997758E-4 4.21008137666293E-5 1.3013151031196282 0.0013472260405321375 88-89 5.894113927328101E-4 2.526048825997758E-4 4.21008137666293E-5 1.4019360480218723 0.001431427668065396 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4455 0.0 51.207527 1 GTATCAA 7800 0.0 39.92116 1 TATCAAC 10075 0.0 30.852213 2 TCAACGC 10035 0.0 30.690145 4 ATCAACG 10155 0.0 30.327482 3 CAACGCA 10260 0.0 30.017115 5 AACGCAG 10725 0.0 28.773645 6 ACGCAGA 12250 0.0 24.999903 7 CGCAGAG 12270 0.0 24.653425 8 GCAGAGT 13350 0.0 21.638634 9 GAGTACT 8505 0.0 20.185942 12-13 AGAGTAC 11490 0.0 19.94967 10-11 AGTACTT 8835 0.0 19.431969 12-13 CAGAGTA 12390 0.0 19.088161 10-11 GTACTTT 9145 0.0 18.56761 14-15 GTACATG 6665 0.0 18.560745 1 TACATGG 6475 0.0 18.011168 2 TGGTATC 2405 0.0 17.988821 2 ACATGGG 6460 0.0 17.104498 3 TACGTCG 170 2.4079418E-7 15.756619 66-67 >>END_MODULE