FastQCFastQC Report
Fri 27 May 2016
SRR939572_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939572_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2294080
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35550.15496408146184962No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24800.10810433812247175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.067.686651
GTATCAA51700.056.2381
TATCAAC78550.038.0108872
ATCAACG83800.035.6859133
TCAACGC85500.034.9740754
CAACGCA86350.034.658015
AACGCAG89100.033.64136
ACGCAGA95500.030.844447
CGCAGAG103650.028.2818958
GTACATG58250.025.750811
GCAGAGT110150.025.6701569
TACATGG63900.021.8101312
GAGTACT64200.021.47103712-13
AGAGTAC95500.021.30464610-11
ACATGGG63750.021.0462743
GTATAGG6401.382432E-1020.8687951
GTGGTAT19250.020.814591
CAGAGTA103200.020.37832310-11
GTACTTT68900.019.93940214-15
AGTACTT70550.019.57194712-13
Produced by FastQC (version 0.11.5)