FastQCFastQC Report
Fri 27 May 2016
SRR939572_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939572_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2294080
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94580.4122785604686846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78250.3410953410517506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61850.2696069884223741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43150.051.8047451
GTATCAA76600.040.2028121
TATCAAC101700.030.1329232
TCAACGC101300.030.0650884
ATCAACG101650.029.8688073
CAACGCA104050.029.2251685
AACGCAG108650.028.0623866
ACGCAGA124800.024.0155537
CGCAGAG124750.023.8362988
GCAGAGT132500.021.3400239
AGAGTAC113700.020.68233710-11
GAGTACT84450.020.28449812-13
GTACATG65550.020.2164841
TACATGG64500.019.8819072
AGTACTT88250.019.6513612-13
CAGAGTA122550.019.34256710-11
GTACTTT89000.018.95625514-15
ACATGGG66400.018.6735573
TACTTTT105050.016.53106914-15
ACTTTTT104650.016.34622416-17
Produced by FastQC (version 0.11.5)