##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939572_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2294080 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.916751813363092 31.0 31.0 33.0 28.0 34.0 2 31.1494267854652 31.0 31.0 34.0 28.0 34.0 3 31.16656611800809 31.0 31.0 34.0 28.0 34.0 4 35.048600746268654 35.0 35.0 37.0 32.0 37.0 5 34.758519755195984 35.0 35.0 37.0 32.0 37.0 6 34.798593335890644 35.0 35.0 37.0 32.0 37.0 7 34.70852978100154 35.0 35.0 37.0 31.0 37.0 8 34.656476234481794 35.0 35.0 37.0 30.0 37.0 9 36.18633526293765 38.0 35.0 39.0 32.0 39.0 10-11 36.02511987376203 37.0 35.0 39.0 30.5 39.0 12-13 35.956923908494915 37.0 35.0 39.0 30.0 39.0 14-15 36.8570130509834 38.0 36.0 40.0 31.0 41.0 16-17 36.769101557051194 38.0 36.0 40.0 31.0 41.0 18-19 36.71665395278281 38.0 35.5 40.0 30.5 41.0 20-21 36.62798812595899 38.0 35.0 40.0 30.0 41.0 22-23 36.447801079299765 38.0 35.0 40.0 30.0 41.0 24-25 36.273827852559634 38.0 34.0 40.0 30.0 41.0 26-27 35.89100336518342 38.0 34.0 40.0 28.5 41.0 28-29 35.62173485667457 38.0 34.0 40.0 27.0 41.0 30-31 35.3107759973497 38.0 34.0 40.0 27.0 41.0 32-33 35.073796467429204 37.5 33.0 40.0 27.0 41.0 34-35 34.99966609708467 38.0 33.0 40.0 26.5 41.0 36-37 34.884775596317475 37.5 33.0 40.0 26.0 41.0 38-39 34.890321174501324 38.0 33.0 40.0 26.0 41.0 40-41 34.71163843457944 38.0 33.0 40.0 25.0 41.0 42-43 34.455827826405354 37.0 33.0 40.0 25.0 41.0 44-45 34.16533577730506 37.0 33.0 40.0 24.0 41.0 46-47 33.79150138617659 37.0 32.5 40.0 23.5 41.0 48-49 33.399326527409684 37.0 31.5 39.5 22.5 40.0 50-51 33.06810202782815 37.0 31.0 39.0 21.5 40.0 52-53 32.74001364381364 36.0 31.0 39.0 19.5 40.0 54-55 32.33605388652532 36.0 31.0 39.0 18.5 40.0 56-57 31.33736356186358 35.5 29.0 38.5 11.5 40.0 58-59 27.842567608801787 33.0 21.0 38.0 4.0 40.0 60-61 27.358300059283025 32.0 20.5 37.0 2.0 39.5 62-63 29.03178616264472 33.5 26.5 37.5 2.0 39.0 64-65 28.98409318768308 34.0 26.5 37.5 2.0 39.0 66-67 23.96331361591575 28.0 15.0 35.5 2.0 38.5 68-69 16.78433620449156 13.0 6.0 28.5 2.0 35.0 70-71 14.982296171014088 12.0 6.0 24.5 2.0 32.5 72-73 16.154428572674014 12.5 5.5 27.0 2.0 34.0 74-75 17.414601713976843 16.0 6.0 29.5 2.0 34.0 76-77 19.63755971892872 24.0 7.0 31.0 2.0 34.5 78-79 21.735547147440368 27.5 7.0 33.5 2.0 36.0 80-81 23.01857563816432 29.5 7.0 34.0 2.0 36.0 82-83 23.812651258892455 30.5 6.5 34.0 2.0 36.0 84-85 23.76275391442321 31.0 2.0 34.0 2.0 35.0 86-87 23.384988099804715 31.0 2.0 34.0 2.0 35.0 88-89 22.780905417422236 30.0 2.0 34.0 2.0 35.0 90-91 20.925933053773193 28.5 2.0 34.0 2.0 35.0 92-93 19.109060276886595 26.0 2.0 33.5 2.0 35.0 94-95 15.555425704421815 4.5 2.0 31.5 2.0 34.0 96-97 13.29152819430883 2.0 2.0 29.5 2.0 34.0 98-99 13.138484926419306 2.0 2.0 30.0 2.0 34.0 100-101 12.319650142976705 2.0 2.0 29.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2634982709854903 1101 2 0.29819622835426074 1101 3 0.21456949261500924 1101 4 0.2910479986050518 1101 5 0.4184353139924255 1101 6 0.3740837604039129 1101 7 0.4065392932045597 1101 8 0.41209083424954684 1101 9 0.4192762529997509 1101 10-11 0.38416381823617485 1101 12-13 0.3214951638078958 1101 14-15 0.4099192365566111 1101 16-17 0.45456704784788826 1101 18-19 0.42768011987030974 1101 20-21 0.491305329532878 1101 22-23 0.3617764100748033 1101 24-25 0.33064392706750567 1101 26-27 0.5086311615189416 1101 28-29 0.5436687231152391 1101 30-31 0.3730724331679056 1101 32-33 0.4647681462478275 1101 34-35 0.3958868208091033 1101 36-37 0.37040650649034035 1101 38-39 0.3300683907317179 1101 40-41 0.3435292455525598 1101 42-43 0.5332745291405416 1101 44-45 0.47803045550602974 1101 46-47 0.25689702730679187 1101 48-49 0.008085251880068256 1101 50-51 0.19607753807305528 1101 52-53 0.09570137154548775 1101 54-55 0.058250716047268725 1101 56-57 -3.9857023050435245 1101 58-59 -10.602286229116245 1101 60-61 -11.260017671107182 1101 62-63 -4.856838782005134 1101 64-65 -1.9349252184574617 1101 66-67 -2.182989598022079 1101 68-69 -3.5500633330711153 1101 70-71 -3.6846872196622744 1101 72-73 -2.95321877408208 1101 74-75 -5.44389006559927 1101 76-77 -4.742360641703909 1101 78-79 0.5298420983793228 1101 80-81 1.5681522335501477 1101 82-83 0.5799597876587654 1101 84-85 0.4572668668467088 1101 86-87 0.45451306959774485 1101 88-89 0.2659854811450728 1101 90-91 1.22049949678863 1101 92-93 -0.04823925320586753 1101 94-95 -8.494684001890032 1101 96-97 -8.502425447747509 1101 98-99 -7.898589629021103 1101 100-101 -7.967913706966976 1102 1 0.2766770102616043 1102 2 0.3865495369977623 1102 3 0.36174497900311664 1102 4 0.313610088989698 1102 5 0.35472957833420793 1102 6 0.3733447558045455 1102 7 0.3092379013509401 1102 8 0.34350523834191193 1102 9 0.3795398330676534 1102 10-11 0.35157392669923127 1102 12-13 0.4572807880290739 1102 14-15 0.5812576378028282 1102 16-17 0.4864904354153907 1102 18-19 0.4751871810883088 1102 20-21 0.3815086059802937 1102 22-23 0.470053261587708 1102 24-25 0.3413337421684446 1102 26-27 0.08802080393897427 1102 28-29 0.24600319057500286 1102 30-31 0.27150763450836024 1102 32-33 0.21754020585206746 1102 34-35 0.23031056889886514 1102 36-37 0.049507229278042075 1102 38-39 0.3446822198901245 1102 40-41 0.24599595733072732 1102 42-43 0.2893589034984032 1102 44-45 0.2597532447914688 1102 46-47 0.32265800395498445 1102 48-49 0.4166788611542316 1102 50-51 0.3995499616859206 1102 52-53 0.2959603929216854 1102 54-55 0.04031479973703256 1102 56-57 -3.896327120490465 1102 58-59 -9.97590851905855 1102 60-61 -8.618969214274504 1102 62-63 -3.095112960635067 1102 64-65 -1.0825210500278004 1102 66-67 -2.1851121893240126 1102 68-69 -4.371296588595028 1102 70-71 -3.8422280511190436 1102 72-73 -2.88158366169686 1102 74-75 -3.654880500798498 1102 76-77 -4.043116232968336 1102 78-79 1.0760732013344416 1102 80-81 1.8020213056001886 1102 82-83 0.8413497818851248 1102 84-85 0.6161277644177154 1102 86-87 0.5253867272796171 1102 88-89 0.44465740694668554 1102 90-91 1.4755729561700548 1102 92-93 0.24726869228210901 1102 94-95 -8.178630777711746 1102 96-97 -8.348482707662136 1102 98-99 -7.716442163003957 1102 100-101 -7.991269474557161 1103 1 0.2849177353205583 1103 2 0.22231578295107823 1103 3 0.3166339744333797 1103 4 0.2711091206514169 1103 5 0.28117373271777524 1103 6 0.31185796127800103 1103 7 0.25623538750894426 1103 8 0.3048679013668405 1103 9 0.41215863423160215 1103 10-11 0.3310738087589584 1103 12-13 0.3121574521766135 1103 14-15 0.2566274099913386 1103 16-17 0.5061927295968189 1103 18-19 0.45448827877713427 1103 20-21 0.42827525154903157 1103 22-23 0.4262392163078559 1103 24-25 0.4956516866408407 1103 26-27 0.4776251232649642 1103 28-29 0.34489265878971054 1103 30-31 0.3683428696393136 1103 32-33 0.3382200995676712 1103 34-35 0.3203449225221888 1103 36-37 0.43805443532149724 1103 38-39 0.5201486979887946 1103 40-41 0.6021450205370016 1103 42-43 0.5225396222842633 1103 44-45 0.47437973228442587 1103 46-47 0.4583376750934889 1103 48-49 0.2709362748902535 1103 50-51 0.34238653534946906 1103 52-53 0.49184358650902027 1103 54-55 0.3797921989518187 1103 56-57 0.6474783542920619 1103 58-59 -8.204777893934146 1103 60-61 -8.22425099431819 1103 62-63 -2.8370870096808254 1103 64-65 -0.6574828409598545 1103 66-67 -1.5573050956129144 1103 68-69 -3.138016221787975 1103 70-71 -3.166302092029225 1103 72-73 -2.5233382804274136 1103 74-75 -3.129941504830981 1103 76-77 -0.009800407327556826 1103 78-79 3.2890156500611205 1103 80-81 3.0849922646618637 1103 82-83 1.9453347305568016 1103 84-85 1.4509128803036297 1103 86-87 1.342630721407673 1103 88-89 1.257708253276899 1103 90-91 2.4674694869862357 1103 92-93 1.0407983761356512 1103 94-95 -6.875390592466202 1103 96-97 -7.611415460214224 1103 98-99 -7.1961309797585375 1103 100-101 -7.614113482807352 1104 1 0.20969758288016394 1104 2 0.1786078983023529 1104 3 0.13609446129229852 1104 4 0.07685035725508982 1104 5 0.31916876267288785 1104 6 0.17656730354970307 1104 7 0.13532755166169608 1104 8 0.18106523911799144 1104 9 0.3947864651987061 1104 10-11 0.25378575805679304 1104 12-13 0.29260289405470274 1104 14-15 0.37851955873308896 1104 16-17 0.32247906582612984 1104 18-19 0.3172573129574303 1104 20-21 0.24606717747353457 1104 22-23 0.36812288900407 1104 24-25 0.32975396425700865 1104 26-27 0.2508581567260748 1104 28-29 0.1804658513693127 1104 30-31 0.29582445671462665 1104 32-33 0.37023560045701487 1104 34-35 0.39271596783692786 1104 36-37 0.22078834528277724 1104 38-39 0.10163810614249513 1104 40-41 0.3033041197645261 1104 42-43 0.23497410951561903 1104 44-45 0.3258134040174596 1104 46-47 0.038605954777303 1104 48-49 -0.057177162852546815 1104 50-51 0.11132304848412389 1104 52-53 0.1282823631331098 1104 54-55 0.1315164320944433 1104 56-57 0.6837251356421916 1104 58-59 -8.531416949240342 1104 60-61 -8.369282233683002 1104 62-63 -2.970198167881076 1104 64-65 -0.7915071646420557 1104 66-67 -1.5981012659814304 1104 68-69 -3.2742683327728024 1104 70-71 -3.3003565313580765 1104 72-73 -2.456828741665559 1104 74-75 -3.17499342060991 1104 76-77 0.08663464497349693 1104 78-79 3.0714032621921383 1104 80-81 2.643837852944376 1104 82-83 1.5208885309716145 1104 84-85 1.1163894331215936 1104 86-87 1.2033677520948132 1104 88-89 0.8194749308596876 1104 90-91 1.9560886621128972 1104 92-93 0.6595283819789834 1104 94-95 -6.535699454428665 1104 96-97 -7.06087581864821 1104 98-99 -5.806467838356266 1104 100-101 -5.294707255482379 1105 1 0.22719171024535356 1105 2 0.2743532711823953 1105 3 0.3820979719662958 1105 4 0.3325293887220866 1105 5 0.3695149254545811 1105 6 0.3923062229848995 1105 7 0.1379565560242213 1105 8 0.33056651690007754 1105 9 0.25545620370511557 1105 10-11 0.17687586105377306 1105 12-13 0.21750112322943238 1105 14-15 0.3754289394605195 1105 16-17 0.5478927961753968 1105 18-19 0.40168276276254034 1105 20-21 0.47137900792863263 1105 22-23 0.5491962904857814 1105 24-25 0.5169264519309351 1105 26-27 0.4112904729964981 1105 28-29 0.5073732315288524 1105 30-31 0.503587111919984 1105 32-33 0.36250379271803723 1105 34-35 0.3748452819648165 1105 36-37 0.4785469993523108 1105 38-39 0.5961534524343435 1105 40-41 0.4378857429349736 1105 42-43 0.3758888207884965 1105 44-45 0.49967895294746967 1105 46-47 0.3462307644006799 1105 48-49 0.3463220885478506 1105 50-51 0.2946716764817552 1105 52-53 0.44133806926904384 1105 54-55 0.3534494969644726 1105 56-57 0.9591082121284238 1105 58-59 -8.028437974563662 1105 60-61 -7.64422931543524 1105 62-63 -2.4784918125285884 1105 64-65 -0.13941676478661336 1105 66-67 -1.3685333886485118 1105 68-69 -3.024690065393676 1105 70-71 -2.8997515209034948 1105 72-73 -2.17380394753045 1105 74-75 -2.7988834926303543 1105 76-77 0.6043390901063077 1105 78-79 3.91389732502288 1105 80-81 3.6219371198330386 1105 82-83 2.5995283384008196 1105 84-85 2.1839723598878997 1105 86-87 1.9958580719275432 1105 88-89 1.822173955098826 1105 90-91 2.727767977531464 1105 92-93 1.2880793254726228 1105 94-95 -6.344634506959705 1105 96-97 -6.832647031121429 1105 98-99 -5.592893468857827 1105 100-101 -4.8847723707409045 1106 1 0.0953140791418221 1106 2 0.15063888823656768 1106 3 0.1999730906547974 1106 4 0.0973517971113722 1106 5 0.1432022096095622 1106 6 0.1982137593897093 1106 7 0.19967142632815182 1106 8 0.24827337100354185 1106 9 0.1959869618830723 1106 10-11 0.162357526305982 1106 12-13 0.2576204629432368 1106 14-15 0.14590060546898087 1106 16-17 0.3430243618552993 1106 18-19 0.26911505779904843 1106 20-21 0.2078616478970403 1106 22-23 0.28236352993825875 1106 24-25 0.19441407891712714 1106 26-27 0.10023994320463459 1106 28-29 0.367812883151835 1106 30-31 0.16764508232557773 1106 32-33 0.3271551006023188 1106 34-35 0.28276821216336856 1106 36-37 0.47759959201337665 1106 38-39 0.4999091557845148 1106 40-41 0.5115834254631793 1106 42-43 0.6493718136713866 1106 44-45 0.6154711409332592 1106 46-47 0.5675701168866283 1106 48-49 0.3535248001074791 1106 50-51 0.27592887131575594 1106 52-53 0.3786290514636619 1106 54-55 0.37555549314963343 1106 56-57 0.7924695545479032 1106 58-59 -8.198268617624272 1106 60-61 -8.080142250591404 1106 62-63 -2.8148198423414605 1106 64-65 -0.42517351427614614 1106 66-67 0.13200018444259953 1106 68-69 -2.106210685682896 1106 70-71 -1.9833497151577113 1106 72-73 -0.8188402473060954 1106 74-75 -1.3149936187552704 1106 76-77 1.8236160985678538 1106 78-79 4.129171484960363 1106 80-81 3.7222383575545557 1106 82-83 2.592058315125268 1106 84-85 2.2937009308363194 1106 86-87 2.1536707163493887 1106 88-89 2.021482935932209 1106 90-91 2.9176884877449822 1106 92-93 1.3271372958782202 1106 94-95 -6.5482556616622904 1106 96-97 -7.186115046145503 1106 98-99 -6.0561893120293515 1106 100-101 -5.469868876047226 1107 1 0.057228753114639375 1107 2 0.001627739532370498 1107 3 -0.021023726334615134 1107 4 0.08774613978102508 1107 5 -0.10418266896669337 1107 6 -0.1511916176440451 1107 7 -0.020739261530998476 1107 8 0.09617367841507019 1107 9 0.06069213321354283 1107 10-11 -0.08047824973116491 1107 12-13 -0.020363187511456715 1107 14-15 -0.05968452726899187 1107 16-17 0.004222123402833233 1107 18-19 0.10737329600323164 1107 20-21 0.09345382888543696 1107 22-23 0.18533595056172913 1107 24-25 0.2076723579570654 1107 26-27 0.11807345966361993 1107 28-29 -0.06129346457492346 1107 30-31 -0.016172077509907012 1107 32-33 -0.057318385017509854 1107 34-35 -0.11907088028114998 1107 36-37 -0.09081029383164463 1107 38-39 0.10780176701837263 1107 40-41 -0.009656609252651549 1107 42-43 -0.09856114872814459 1107 44-45 -0.07362029630001388 1107 46-47 -0.09040726936402876 1107 48-49 -0.10485485911546277 1107 50-51 -0.15087236638859736 1107 52-53 -0.15682992330854972 1107 54-55 -0.08592625381463392 1107 56-57 0.251901823284161 1107 58-59 -9.11199663029533 1107 60-61 -8.708309675532572 1107 62-63 -3.481168217024166 1107 64-65 -1.186970009588748 1107 66-67 -0.33290802223638494 1107 68-69 -2.8237636877638863 1107 70-71 -2.753429061742155 1107 72-73 -1.7364388084720606 1107 74-75 -1.734856930610814 1107 76-77 1.004868705249887 1107 78-79 3.0994943759572244 1107 80-81 2.6492449310213395 1107 82-83 1.534559877773301 1107 84-85 1.234567157819928 1107 86-87 1.0460447625623885 1107 88-89 0.9630732488211713 1107 90-91 1.948283039929258 1107 92-93 0.577988721263683 1107 94-95 -6.797929928497236 1107 96-97 -7.34766753424052 1107 98-99 -6.235359091287622 1107 100-101 -5.6943956033177745 1108 1 -0.10361007156763336 1108 2 -0.11071139724021961 1108 3 -0.11744772697673156 1108 4 -0.12805644978948294 1108 5 -0.046628245736940244 1108 6 -0.09185109109196787 1108 7 -0.07578755394272463 1108 8 -0.35546040766893583 1108 9 -0.26648891944959985 1108 10-11 -0.21976284993361617 1108 12-13 -0.11108459390832337 1108 14-15 -0.3178470929542243 1108 16-17 -0.26497444469151077 1108 18-19 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2311 20-21 -0.04571622972787992 2311 22-23 -0.2689785130410627 2311 24-25 -0.19584096346814306 2311 26-27 -0.2206298505738289 2311 28-29 -0.17039927420642442 2311 30-31 -0.24938186697239928 2311 32-33 -0.23101443575283298 2311 34-35 -0.06913971421167986 2311 36-37 -0.1057683158799918 2311 38-39 0.03525771449682935 2311 40-41 -0.04609754878505612 2311 42-43 -0.02798271386725304 2311 44-45 -0.07646245705262089 2311 46-47 0.023427440249918163 2311 48-49 -0.11018605599006293 2311 50-51 0.16726240423859906 2311 52-53 -0.02930439565139409 2311 54-55 0.017265613751987985 2311 56-57 0.8315745606298819 2311 58-59 3.704514005830479 2311 60-61 4.0243057272683025 2311 62-63 1.7239745820392613 2311 64-65 0.9345036693430551 2311 66-67 -1.5056258162551792 2311 68-69 -3.6214421545045035 2311 70-71 -3.4697436435506557 2311 72-73 -5.173856348050078 2311 74-75 -6.201116215430455 2311 76-77 -4.8579734196828 2311 78-79 -0.9903842005117198 2311 80-81 1.1208130998955035 2311 82-83 0.6444252187848285 2311 84-85 0.48993611485155597 2311 86-87 0.5596387175940762 2311 88-89 0.5001364846964833 2311 90-91 1.6978202168187266 2311 92-93 1.3226907324323314 2311 94-95 -2.9471356816526733 2311 96-97 -6.394338565961441 2311 98-99 -6.186799230841999 2311 100-101 -6.40011823720365 2312 1 -0.21459968889585923 2312 2 -0.051439680640410046 2312 3 -0.14709423934682775 2312 4 -0.10755177048981324 2312 5 -0.22853071341185682 2312 6 -0.2965750577512978 2312 7 -0.2090058874026255 2312 8 -0.20341593165213112 2312 9 -0.2555559208943876 2312 10-11 -0.1826258052669516 2312 12-13 -0.2232520867861183 2312 14-15 -0.3267267397710043 2312 16-17 -0.35906117939683213 2312 18-19 -0.33692223283684797 2312 20-21 -0.4176429592111077 2312 22-23 -0.24687720214333098 2312 24-25 -0.40811158239040424 2312 26-27 -0.2999266223873178 2312 28-29 -0.31009579890904604 2312 30-31 -0.3254004185045858 2312 32-33 -0.16328236104961746 2312 34-35 -0.19801733122498177 2312 36-37 -0.21957391094350243 2312 38-39 -0.3578282076014361 2312 40-41 -0.22525243355124047 2312 42-43 -0.28006856569702876 2312 44-45 -0.42582356682631683 2312 46-47 -0.33944042831156906 2312 48-49 -0.2633511960520778 2312 50-51 -0.14656131098662684 2312 52-53 -0.24489747797873918 2312 54-55 -0.19725457282386571 2312 56-57 0.6141095224571771 2312 58-59 3.594515433972493 2312 60-61 3.691583447862566 2312 62-63 1.1725609003737425 2312 64-65 0.6423975535119553 2312 66-67 -1.4185685240124286 2312 68-69 -4.002552829329254 2312 70-71 -3.6163922757224327 2312 72-73 -5.704368162916028 2312 74-75 -6.647916298971882 2312 76-77 -4.935468016165991 2312 78-79 -1.2994873208230864 2312 80-81 0.6668492901201155 2312 82-83 0.42165621083277927 2312 84-85 0.42646333543345705 2312 86-87 0.404407488715286 2312 88-89 0.5891836602284748 2312 90-91 1.948965820939062 2312 92-93 3.1700640734778496 2312 94-95 4.04458922761639 2312 96-97 -1.4897993678550474 2312 98-99 -2.6472465288233415 2312 100-101 -2.020850808991371 2313 1 -0.3574599753321763 2313 2 -0.42813626204769406 2313 3 -0.190223483799155 2313 4 -0.30070667647528637 2313 5 -0.3240271887977926 2313 6 -0.2987553780853247 2313 7 -0.5450851095686318 2313 8 -0.5861438833323405 2313 9 -0.3857839825998681 2313 10-11 -0.472671115824447 2313 12-13 -0.5318143195612066 2313 14-15 -0.5507901434466049 2313 16-17 -0.6240323924841817 2313 18-19 -0.4882719202823509 2313 20-21 -0.437323171174377 2313 22-23 -0.392368002541609 2313 24-25 -0.5896861217815967 2313 26-27 -0.5955054595476028 2313 28-29 -0.5018403960064362 2313 30-31 -0.7273616975508332 2313 32-33 -0.7322440500847804 2313 34-35 -0.6786504844698342 2313 36-37 -0.5250957235775857 2313 38-39 -0.6259236420324967 2313 40-41 -0.7816093628104497 2313 42-43 -0.6424426694874228 2313 44-45 -0.6481015002572192 2313 46-47 -0.7422200306521205 2313 48-49 -0.8820893931531657 2313 50-51 -0.7417788350838208 2313 52-53 -0.6470159359836742 2313 54-55 -0.7285939890380533 2313 56-57 0.3211263717106405 2313 58-59 3.4433584148400094 2313 60-61 3.295033840363242 2313 62-63 0.43744880861299507 2313 64-65 -0.3139898353749295 2313 66-67 -1.8963362221149964 2313 68-69 -4.068532916244255 2313 70-71 -3.00086259752236 2313 72-73 -3.12852608905383 2313 74-75 -4.103833888223885 2313 76-77 -0.38703753906663607 2313 78-79 2.0583787249811785 2313 80-81 1.824187826793942 2313 82-83 0.8265133053780751 2313 84-85 0.5298973482321223 2313 86-87 0.5030347207805903 2313 88-89 0.8156093809270892 2313 90-91 2.171219598445795 2313 92-93 3.2115152257319757 2313 94-95 4.0847938680025155 2313 96-97 -0.7080828048658265 2313 98-99 0.7558047449383949 2313 100-101 1.7715698065346661 2314 1 -0.26571825157881435 2314 2 -0.26693697164234464 2314 3 -0.3894562581584644 2314 4 -0.21031805849085572 2314 5 -0.301678415132848 2314 6 -0.33379501947387524 2314 7 -0.34939838743417084 2314 8 -0.45945752200280054 2314 9 -0.4178783311653689 2314 10-11 -0.46671137299138366 2314 12-13 -0.5515582598373427 2314 14-15 -0.6992629396986985 2314 16-17 -0.5322870886239244 2314 18-19 -0.41447325571461846 2314 20-21 -0.512096847294508 2314 22-23 -0.47192059745547965 2314 24-25 -0.5867885928237797 2314 26-27 -0.6738806358896952 2314 28-29 -0.4916156106993341 2314 30-31 -0.524685296951894 2314 32-33 -0.423571773768991 2314 34-35 -0.3945441158263989 2314 36-37 -0.40013479913460515 2314 38-39 -0.37541020231119404 2314 40-41 -0.3977492531299376 2314 42-43 -0.54982879676529 2314 44-45 -0.3787058781865795 2314 46-47 -0.41237264449689803 2314 48-49 -0.49720633341567577 2314 50-51 -0.5610810600242075 2314 52-53 -0.5740410452817173 2314 54-55 -0.28465837577621755 2314 56-57 0.487193123065758 2314 58-59 3.6254866609054694 2314 60-61 3.9229402322686404 2314 62-63 1.4569104443620908 2314 64-65 0.466924475028371 2314 66-67 -1.6398085520800016 2314 68-69 -3.51819312043958 2314 70-71 -3.091981731953803 2314 72-73 -3.3198100655781086 2314 74-75 -4.577522023540052 2314 76-77 -0.022568860112109235 2314 78-79 2.9436010216773845 2314 80-81 2.613376550120833 2314 82-83 1.5006217627082634 2314 84-85 1.2347494933099306 2314 86-87 1.326688347359024 2314 88-89 1.4065924712963245 2314 90-91 2.769380279231939 2314 92-93 4.2025397230937 2314 94-95 5.599467852989816 2314 96-97 2.5181072139358474 2314 98-99 4.652108345687667 2314 100-101 4.9248802004058 2315 1 -0.4051278926398787 2315 2 -0.33265007382828315 2315 3 -0.49635834770548115 2315 4 -0.3311101410883097 2315 5 -0.478868829683492 2315 6 -0.390518767383206 2315 7 -0.5604965360056724 2315 8 -0.6595037002577158 2315 9 -0.8004537616710863 2315 10-11 -0.45090606644802733 2315 12-13 -0.4662124573608324 2315 14-15 -0.6128049982370598 2315 16-17 -0.47374340776054424 2315 18-19 -0.5819409413966596 2315 20-21 -0.612985873796795 2315 22-23 -0.629706445227761 2315 24-25 -0.4360198237785724 2315 26-27 -0.44234537628155834 2315 28-29 -0.495565772418324 2315 30-31 -0.4824688053809467 2315 32-33 -0.58030044761243 2315 34-35 -0.49261138088311895 2315 36-37 -0.33211050191705027 2315 38-39 -0.5532213035788871 2315 40-41 -0.36556474019071317 2315 42-43 -0.34565339242978155 2315 44-45 -0.47445510135345614 2315 46-47 -0.4920629420803735 2315 48-49 -0.3815180130527054 2315 50-51 -0.2588135707709256 2315 52-53 -0.025501099759878798 2315 54-55 -0.10883333886974356 2315 56-57 0.7998926566805977 2315 58-59 3.874760038076449 2315 60-61 4.122285558508121 2315 62-63 1.542437308200526 2315 64-65 0.6902996109670347 2315 66-67 -1.0510610022091988 2315 68-69 -3.677524170979666 2315 70-71 -2.979807086098033 2315 72-73 -3.072539394437653 2315 74-75 -1.1043142552468623 2315 76-77 1.7471448613341103 2315 78-79 3.729703744330557 2315 80-81 3.160015161151957 2315 82-83 1.8675707608417333 2315 84-85 1.55246349471658 2315 86-87 1.4572480437845456 2315 88-89 1.6608825985451823 2315 90-91 2.9766571201524137 2315 92-93 4.218819935331087 2315 94-95 5.767903988729541 2315 96-97 2.281887371968944 2315 98-99 4.8038980573702705 2315 100-101 5.029392926623144 2316 1 -0.35551812402228933 2316 2 -0.4404322539908101 2316 3 -0.44258382774743765 2316 4 -0.540471320307546 2316 5 -0.6641360991861873 2316 6 -0.6911116450930948 2316 7 -0.3841046887344035 2316 8 -0.4525680048766958 2316 9 -0.7769895369387427 2316 10-11 -0.7263998492372465 2316 12-13 -0.6532451968536321 2316 14-15 -0.7137609145112407 2316 16-17 -0.7752044749899412 2316 18-19 -0.7446241421770381 2316 20-21 -0.6413988613178034 2316 22-23 -0.8665614617043715 2316 24-25 -0.7564453026651421 2316 26-27 -0.9313087645974747 2316 28-29 -0.6782288860001202 2316 30-31 -0.7181112862572547 2316 32-33 -0.5807046261458666 2316 34-35 -0.35670650375421786 2316 36-37 -0.42346434113859033 2316 38-39 -0.6086405970277795 2316 40-41 -0.697388713940363 2316 42-43 -0.5786114593711531 2316 44-45 -0.6658706711605973 2316 46-47 -0.5157658776668157 2316 48-49 -0.5010013577610763 2316 50-51 -0.29939441596827976 2316 52-53 -0.28302699070636805 2316 54-55 0.001941082880549061 2316 56-57 0.800554114760331 2316 58-59 3.9510212442821846 2316 60-61 4.280200938089326 2316 62-63 1.879421934148663 2316 64-65 1.1877585885996034 2316 66-67 -0.6947407970787971 2316 68-69 -2.407561588957625 2316 70-71 -2.085719605275367 2316 72-73 -0.9007344267760633 2316 74-75 5.224667677002994 2316 76-77 6.307391602115995 2316 78-79 5.457158257224247 2316 80-81 4.18495992960862 2316 82-83 2.9148681083246863 2316 84-85 2.633189439801118 2316 86-87 2.409139863409095 2316 88-89 2.44910171241418 2316 90-91 3.6736191583180897 2316 92-93 4.950359147869992 2316 94-95 6.504173885083231 2316 96-97 2.764901970262109 2316 98-99 4.971284501564748 2316 100-101 5.071329698988309 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 23.0 4 72.0 5 125.0 6 391.0 7 931.0 8 2102.0 9 3550.0 10 5579.0 11 8534.0 12 11845.0 13 15945.0 14 20874.0 15 28654.0 16 37211.0 17 48601.0 18 53228.0 19 55935.0 20 59277.0 21 60066.0 22 59758.0 23 59804.0 24 60659.0 25 57988.0 26 70164.0 27 97393.0 28 133416.0 29 165898.0 30 183218.0 31 183712.0 32 174268.0 33 163567.0 34 158094.0 35 144856.0 36 106475.0 37 51205.0 38 10384.0 39 271.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.33415767549463 22.365760268161182 16.37381591648914 21.926266139855056 2 17.070379628523625 23.018090793129875 37.95483099769211 21.956698580654386 3 19.934588100317427 26.120864242628194 31.113240264716367 22.83130739233801 4 14.696741177291115 19.553241386525315 36.930621425582366 28.8193960106012 5 13.219702866971328 38.40706274228134 35.05218166047185 13.32105273027548 6 30.561968196401175 38.9553982424327 17.204543869437856 13.278089691728272 7 26.64806807086065 33.43998465615846 22.11579369507602 17.796153577904867 8 25.160914319391058 36.7382887591442 20.086762525075432 18.014034396389313 9 25.680185520993167 16.4132026781978 20.548498744594784 37.35811305621425 10-11 24.323628644162365 27.386751987724928 28.34685364067513 19.94276572743758 12-13 25.280918712512207 24.673158739015204 28.554780129725206 21.491142418747387 14-15 22.345264093946565 26.003195194566864 26.66950005884738 24.982040652639196 16-17 21.574596352350397 28.94999302552657 27.456954421816153 22.018456200306876 18-19 21.57131040976255 27.716146721605355 29.592961861493045 21.11958100713905 20-21 22.419205084391127 26.957168015064862 29.67119716836379 20.95242973218022 22-23 22.01732284837495 26.887292509415538 29.47177081880318 21.62361382340633 24-25 21.944235997155285 27.055329623637554 29.649007215751332 21.351427163455828 26-27 21.901473728994016 27.177230421713993 29.487330097902053 21.433965751389938 28-29 21.884940086493057 27.26573489673535 29.546388777611014 21.302936239160577 30-31 22.071407771391904 27.140018622883126 29.477970768350865 21.310602837374105 32-33 22.00575426665795 27.08873340744131 29.43111227358922 21.474400052311516 34-35 22.143125851937 27.234412522553036 29.418008515119855 21.204453110390112 36-37 22.204475944269564 27.00807568333167 29.408795945211764 21.378652427187006 38-39 21.997083677009595 27.332327784933614 29.326471585042917 21.344116953013874 40-41 22.19818020780239 27.144735616721288 29.352507912163695 21.30457626331262 42-43 22.06423484071064 27.156652442422345 29.19521603002904 21.583896686837974 44-45 22.15744317765424 27.339963043227904 29.07383851391201 21.428755265205844 46-47 22.308204015310963 27.377986673791426 28.62214418914056 21.691665121757055 48-49 22.252219165582403 27.520030392896228 28.657561301573164 21.57018913994821 50-51 22.24051173414668 27.589714199479836 28.471863301454626 21.697910764918856 52-53 22.36462508727851 27.638536782577184 28.303859618211845 21.692978511932463 54-55 22.447437281999388 27.62018828755225 28.235523189476098 21.696851240972265 56-57 22.40633031540782 27.665271988428476 28.40652922281293 21.521868473350768 58-59 23.23889062571745 26.386624481871575 29.723605521342595 20.65087937106838 60-61 22.582387366636087 27.259196843758982 28.950168368728608 21.208247420876322 62-63 22.28408856355902 27.590312785866345 28.52232560967928 21.603273040895356 64-65 22.2053523984138 27.636454861015757 28.39779829183226 21.760394448738186 66-67 24.35794631034705 25.411480704205847 30.277463878001637 19.953109107445467 68-69 25.752775784562903 23.127365312696295 32.774175088465206 18.34568381427559 70-71 26.6254049787902 22.690406210788012 32.47403254663441 18.210156263787376 72-73 25.275983114863365 24.005354365696512 31.445123305932015 19.27353921350811 74-75 24.395995076805065 25.155087047362223 30.316620820095046 20.132297055737663 76-77 22.301714871104615 26.682681853345287 29.6750851461157 21.340518129434397 78-79 23.00113841409906 26.482278511814584 29.43123796888335 21.08534510520301 80-81 22.230757473111236 27.56313547856192 28.132276570910747 22.0738304774161 82-83 22.25255396944129 27.69751307280142 28.006264831679506 22.043668126077776 84-85 22.24441974769943 27.680897905646763 27.8287805036465 22.24590184300731 86-87 22.07519933637003 27.67649521076158 27.98965323110944 22.258652221758947 88-89 22.301808765480345 27.63556260171568 27.857372796964007 22.205255835839967 90-91 22.68590672546173 26.87352398132567 28.936439830928474 21.504129462284126 92-93 22.19185287910605 27.5046947931903 27.9985231383354 22.304929189368252 94-95 22.73209016962902 26.037932619185455 30.03600540202142 21.193971809164104 96-97 23.61528858745994 24.662955293165677 31.536227370647584 20.185528748726796 98-99 22.519123919222356 26.40936389064372 29.200893257799493 21.870618932334427 100-101 22.148770588508768 26.577257181252563 29.355154352961403 21.91881787727727 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 553.0 1 467.0 2 386.5 3 404.5 4 432.0 5 489.0 6 609.5 7 975.0 8 1430.5 9 1805.5 10 2013.5 11 2101.5 12 2318.0 13 2423.5 14 2525.5 15 2814.0 16 3109.5 17 3405.5 18 3774.0 19 4173.0 20 4757.5 21 5518.5 22 6508.5 23 7906.0 24 9622.5 25 11872.0 26 14902.0 27 18652.5 28 22571.5 29 26705.5 30 30887.0 31 35304.0 32 40631.5 33 46080.0 34 51445.0 35 56910.5 36 62272.5 37 66639.5 38 69781.5 39 72336.0 40 75010.5 41 77824.0 42 79528.0 43 81101.5 44 82960.0 45 84658.5 46 85895.5 47 86319.0 48 86313.0 49 85284.0 50 83211.0 51 80459.0 52 77572.5 53 74482.5 54 70047.5 55 64622.0 56 59034.0 57 52770.5 58 46154.5 59 39464.5 60 32269.5 61 25310.5 62 19311.0 63 14159.5 64 10141.0 65 7294.0 66 5142.5 67 3614.5 68 2511.0 69 1685.5 70 1110.0 71 656.0 72 363.5 73 236.5 74 142.0 75 65.5 76 39.5 77 33.5 78 18.5 79 10.0 80 6.0 81 3.5 82 2.0 83 1.0 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02327730506346771 2 0.008979634537592412 3 8.718091784070302E-5 4 0.0 5 0.04502894406472312 6 0.0 7 0.0 8 8.718091784070302E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 4.359045892035151E-4 16-17 0.0 18-19 3.269284419026364E-4 20-21 0.0 22-23 0.0 24-25 6.538568838052728E-5 26-27 6.538568838052728E-5 28-29 0.0010025805551680848 30-31 0.005579578741804993 32-33 0.005666759659645697 34-35 0.0018743897335751151 36-37 0.06889472032361556 38-39 0.06394720323615567 40-41 0.0682408634398103 42-43 0.07116142418747386 44-45 0.07288324731482773 46-47 0.06996268656716417 48-49 0.06272667038638582 50-51 0.05784453898730646 52-53 0.04938798995675826 54-55 0.040909645696749895 56-57 0.06782675408006696 58-59 0.7942399567582646 60-61 0.36280338959408565 62-63 0.002898765518203376 64-65 0.0018743897335751151 66-67 1.1726705258752963 68-69 2.4988230576091506 70-71 2.43433097363649 72-73 1.899672199748919 74-75 1.3476208327521273 76-77 0.7715075324313014 78-79 0.5786851373971266 80-81 0.0425224926768029 82-83 1.7436183568140604E-4 84-85 0.0013295089970707212 86-87 0.0013295089970707212 88-89 6.538568838052728E-5 90-91 0.3464133770400335 92-93 0.06017662853954526 94-95 1.0221308759938625 96-97 1.7404798437717952 98-99 0.6736687473845725 100-101 0.6122715859952573 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2294080.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.76794518171995 #Duplication Level Percentage of deduplicated Percentage of total 1 78.01125529922307 46.62572430281124 2 12.652398757698474 15.124157507347679 3 4.109329291517348 7.36818503487135 4 1.8181976496668073 4.346797498192712 5 0.9419644350364184 2.814963935819323 6 0.5567377307119494 1.9965042101872172 7 0.3814977256848723 1.5960934608979003 8 0.26270613559807265 1.2561124729061675 9 0.18849112490914463 1.013915449872944 >10 0.981046930636126 10.681044200597487 >50 0.0665664371051796 2.728784878409856 >100 0.028789442211956284 3.010902728322864 >500 6.550971432909096E-4 0.28725845379242637 >1k 1.455771429533773E-4 0.1285985142222882 >5k 2.1836571443006598E-4 1.0209573517485317 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9458 0.4122785604686846 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7825 0.3410953410517506 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6185 0.2696069884223741 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 8.718091784070303E-5 0.0 0.0 0.0 0.0 8 8.718091784070303E-5 0.0 0.0 0.0 0.0 9 8.718091784070303E-5 0.0 0.0 0.0 0.0 10-11 8.718091784070303E-5 0.0 0.0 2.179522946017576E-5 0.0 12-13 8.718091784070303E-5 0.0 0.0 6.538568838052728E-5 0.0 14-15 8.718091784070303E-5 0.0 0.0 8.718091784070303E-5 8.718091784070303E-5 16-17 8.718091784070303E-5 0.0 0.0 1.3077137676105455E-4 1.3077137676105455E-4 18-19 1.3077137676105455E-4 0.0 0.0 2.1795229460175758E-4 1.3077137676105455E-4 20-21 1.7436183568140607E-4 0.0 0.0 3.051332124424606E-4 1.525666062212303E-4 22-23 1.7436183568140607E-4 0.0 0.0 4.5769981866369087E-4 3.051332124424606E-4 24-25 1.7436183568140607E-4 0.0 0.0 6.102664248849212E-4 3.051332124424606E-4 26-27 1.7436183568140607E-4 0.0 0.0 8.718091784070302E-4 3.4872367136281213E-4 28-29 2.1795229460175756E-4 0.0 0.0 0.0014384851443716 3.4872367136281213E-4 30-31 2.1795229460175756E-4 0.0 0.0 0.0031603082717254847 3.7051890082298784E-4 32-33 2.1795229460175756E-4 0.0 0.0 0.007039859115636769 4.7949504812386663E-4 34-35 2.1795229460175756E-4 0.0 0.0 0.013752789789370903 4.7949504812386663E-4 36-37 3.7051890082298784E-4 0.0 0.0 0.02377859534105175 4.7949504812386663E-4 38-39 4.359045892035151E-4 0.0 0.0 0.03927500348723671 5.230855070442182E-4 40-41 4.359045892035151E-4 0.0 0.0 0.057539405774863994 5.230855070442182E-4 42-43 4.5769981866369087E-4 0.0 0.0 0.07746024550146463 5.230855070442182E-4 44-45 5.230855070442182E-4 0.0 0.0 0.10335297810015344 5.666759659645697E-4 46-47 5.448807365043939E-4 0.0 0.0 0.1339534802622402 6.102664248849212E-4 48-49 5.666759659645697E-4 0.0 0.0 0.16213471195424745 6.974473427256243E-4 50-51 5.666759659645697E-4 0.0 0.0 0.19175442879062632 7.410378016459757E-4 52-53 5.666759659645697E-4 0.0 0.0 0.2246652252754917 7.846282605663272E-4 54-55 5.884711954247455E-4 0.0 0.0 0.2622184056353746 8.282187194866787E-4 56-57 6.538568838052728E-4 0.0 0.0 0.29399585018831076 8.282187194866787E-4 58-59 6.538568838052728E-4 0.0 0.0 0.31709879341609704 8.282187194866787E-4 60-61 6.538568838052728E-4 0.0 0.0 0.33682347607755614 8.282187194866787E-4 62-63 7.846282605663272E-4 2.179522946017576E-5 0.0 0.35467376900544006 8.282187194866787E-4 64-65 7.846282605663272E-4 4.359045892035152E-5 0.0 0.37446383735527966 8.282187194866787E-4 66-67 7.846282605663272E-4 4.359045892035152E-5 0.0 0.39390518203375646 8.282187194866787E-4 68-69 8.282187194866787E-4 4.359045892035152E-5 0.0 0.41757480122750734 8.718091784070302E-4 70-71 8.500139489468544E-4 4.359045892035152E-5 0.0 0.45541131957037245 8.93604407867206E-4 72-73 8.718091784070302E-4 4.359045892035152E-5 0.0 0.5146507532431301 9.371948667875574E-4 74-75 8.718091784070302E-4 4.359045892035152E-5 0.0 0.5992380387780722 9.589900962477333E-4 76-77 8.718091784070302E-4 4.359045892035152E-5 0.0 0.7174335681406054 0.0010461710140884364 78-79 8.718091784070302E-4 4.359045892035152E-5 0.0 0.8622410726740131 0.0010461710140884364 80-81 9.589900962477333E-4 4.359045892035152E-5 0.0 1.0341618426558794 0.0010461710140884364 82-83 9.589900962477333E-4 4.359045892035152E-5 0.0 1.2101147300878785 0.0010461710140884364 84-85 9.589900962477333E-4 4.359045892035152E-5 0.0 1.3609595131817547 0.0010461710140884364 86-87 9.589900962477333E-4 4.359045892035152E-5 0.0 1.5016913098061098 0.0011333519319291394 88-89 9.589900962477333E-4 4.359045892035152E-5 0.0 1.6478937090249688 0.0012205328497698425 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4315 0.0 51.804745 1 GTATCAA 7660 0.0 40.202812 1 TATCAAC 10170 0.0 30.132923 2 TCAACGC 10130 0.0 30.065088 4 ATCAACG 10165 0.0 29.868807 3 CAACGCA 10405 0.0 29.225168 5 AACGCAG 10865 0.0 28.062386 6 ACGCAGA 12480 0.0 24.015553 7 CGCAGAG 12475 0.0 23.836298 8 GCAGAGT 13250 0.0 21.340023 9 AGAGTAC 11370 0.0 20.682337 10-11 GAGTACT 8445 0.0 20.284498 12-13 GTACATG 6555 0.0 20.216484 1 TACATGG 6450 0.0 19.881907 2 AGTACTT 8825 0.0 19.65136 12-13 CAGAGTA 12255 0.0 19.342567 10-11 GTACTTT 8900 0.0 18.956255 14-15 ACATGGG 6640 0.0 18.673557 3 TACTTTT 10505 0.0 16.531069 14-15 ACTTTTT 10465 0.0 16.346224 16-17 >>END_MODULE