FastQCFastQC Report
Fri 27 May 2016
SRR939571_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939571_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3374053
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121730.3607827144386884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81550.24169744814322713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60880.18043581413807072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA110450.039.999891
GGTATCA63700.039.8555831
TCAACGC136400.032.182154
ATCAACG137300.032.0052873
TATCAAC138450.031.8079932
CAACGCA140100.031.2978925
AACGCAG144950.030.474876
ACGCAGA161800.027.0079447
CGCAGAG160850.026.6380758
GTACATG98800.024.3034741
TACATGG98900.023.89542
GCAGAGT171300.023.7111459
ACATGGG104000.021.5372333
AGAGTAC140900.021.09792910-11
GAGTACT91650.021.0714712-13
AGTACTT99100.020.39712112-13
GTACTTT104000.018.57061214-15
CAGAGTA162100.018.42648310-11
CATGGGG60850.017.7812374
CATGGGA56300.016.6052534
Produced by FastQC (version 0.11.5)