##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939571_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3374053 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.635536549070213 31.0 31.0 33.0 27.0 34.0 2 30.811808824579817 31.0 31.0 34.0 27.0 34.0 3 30.873715676665423 31.0 31.0 34.0 27.0 34.0 4 34.485368190718994 37.0 35.0 37.0 30.0 37.0 5 34.47932471718731 37.0 35.0 37.0 30.0 37.0 6 34.58825424496889 37.0 35.0 37.0 30.0 37.0 7 34.41752663636286 37.0 35.0 37.0 30.0 37.0 8 34.4224343838108 37.0 35.0 37.0 30.0 37.0 9 35.769394256699584 38.0 35.0 39.0 30.0 39.0 10-11 35.66410115668012 38.0 35.0 39.0 30.0 39.0 12-13 35.52128537992735 38.0 35.0 39.0 30.0 39.0 14-15 36.3012015519614 38.0 35.5 40.0 29.5 41.0 16-17 36.02808225004171 38.0 35.0 40.0 27.5 41.0 18-19 35.97587945417574 38.0 35.0 40.0 27.0 41.0 20-21 36.10933097375768 39.0 35.0 40.0 27.5 41.0 22-23 35.97012984087684 39.0 35.0 40.0 27.0 41.0 24-25 35.860393716399834 38.0 35.0 40.0 27.0 41.0 26-27 36.00984305818551 39.0 35.0 40.0 27.0 41.0 28-29 35.90393156242655 39.0 35.0 40.0 27.0 41.0 30-31 35.70389676747816 38.0 35.0 40.0 27.0 41.0 32-33 35.491426779603046 38.0 34.0 40.0 27.0 41.0 34-35 35.27930488940156 38.0 34.0 40.0 25.5 41.0 36-37 35.01843628419589 38.0 34.0 40.0 25.0 41.0 38-39 34.872208290741135 38.0 34.0 40.0 25.0 41.0 40-41 34.615256488265004 38.0 33.0 40.0 24.5 41.0 42-43 34.316273336548065 38.0 33.0 40.0 24.0 41.0 44-45 34.07303545617096 38.0 33.0 40.0 23.5 41.0 46-47 33.64338349160491 38.0 32.5 40.0 21.0 41.0 48-49 33.300834930571625 37.5 32.0 40.0 19.5 41.0 50-51 32.71072623933293 36.5 31.0 39.5 18.0 40.5 52-53 32.78447404353162 37.0 31.5 39.0 18.0 40.0 54-55 32.83513714811237 37.0 31.0 40.0 17.0 40.5 56-57 32.69904503574781 37.0 31.0 39.5 16.5 41.0 58-59 32.30018348852256 36.0 31.0 39.0 13.5 40.0 60-61 31.890985855883116 36.0 31.0 39.0 11.5 40.0 62-63 31.47547193242074 35.5 30.0 39.0 8.0 40.0 64-65 30.86247622666271 35.0 29.0 38.0 7.5 40.0 66-67 30.325402712998283 34.5 28.5 38.0 4.0 40.0 68-69 29.805392950258934 34.0 28.0 37.5 2.0 39.0 70-71 29.254283201834706 34.0 27.5 37.0 2.0 39.0 72-73 28.51012787884482 33.5 26.0 36.0 2.0 39.0 74-75 28.048733822497752 33.0 26.0 36.0 2.0 38.0 76-77 27.667857173553585 33.0 26.0 35.0 2.0 37.5 78-79 27.095249244751045 32.0 25.0 35.0 2.0 37.0 80-81 26.4223472482501 32.0 23.5 35.0 2.0 36.0 82-83 25.754888705067764 31.0 21.5 34.0 2.0 36.0 84-85 25.097954151876095 31.0 20.0 34.0 2.0 35.0 86-87 24.42496650171174 31.0 17.5 34.0 2.0 35.0 88-89 23.62731794669497 30.0 9.5 34.0 2.0 35.0 90-91 22.338782319068493 29.5 2.0 34.0 2.0 35.0 92-93 20.54932361761952 26.5 2.0 33.0 2.0 34.5 94-95 20.34278373813334 26.5 2.0 33.0 2.0 34.0 96-97 19.72785741658474 26.5 2.0 33.0 2.0 34.0 98-99 18.696452752816864 25.0 2.0 32.0 2.0 34.0 100-101 16.978526122737257 21.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0027802579787881 1101 2 0.8785926390379508 1101 3 0.9518976818438851 1101 4 0.9445409898868462 1101 5 0.7330874830976128 1101 6 0.7653063753512441 1101 7 0.8402445038038024 1101 8 0.8830872777646732 1101 9 1.175425698636026 1101 10-11 0.9904111741363195 1101 12-13 0.8985812026145155 1101 14-15 1.1826090319343905 1101 16-17 1.055435031104544 1101 18-19 0.9856816078874289 1101 20-21 1.0953086284199003 1101 22-23 1.3296212961104388 1101 24-25 1.1756996094048446 1101 26-27 1.127985945017791 1101 28-29 1.160071100895344 1101 30-31 1.1413976610356187 1101 32-33 1.066965612954121 1101 34-35 1.137909618343528 1101 36-37 1.0696664875535475 1101 38-39 1.190693219097767 1101 40-41 1.200189086802368 1101 42-43 1.1782686590457203 1101 44-45 1.2094939184024867 1101 46-47 1.2079924499341956 1101 48-49 1.1014591577278239 1101 50-51 1.0630184491622927 1101 52-53 1.181654159120555 1101 54-55 1.0935154508797638 1101 56-57 1.1790130454363776 1101 58-59 1.1364008848623612 1101 60-61 0.8845065765589837 1101 62-63 0.9570255655975544 1101 64-65 0.8075022637173497 1101 66-67 0.6587201504380573 1101 68-69 0.6630571789552135 1101 70-71 0.7629650282610605 1101 72-73 0.8833372039650023 1101 74-75 0.4251403402708114 1101 76-77 -0.012112676638810882 1101 78-79 0.20690319966922388 1101 80-81 0.12138976768037324 1101 82-83 0.18925668719004562 1101 84-85 -0.1386082378967437 1101 86-87 -0.4786833753965958 1101 88-89 -1.1462362325590227 1101 90-91 -4.569318714016621 1101 92-93 -10.100712798197627 1101 94-95 -8.3085162755957 1101 96-97 -4.974964147172781 1101 98-99 -3.54067664485704 1101 100-101 -3.1067813655218757 1102 1 0.8031612495362488 1102 2 0.7846424715583709 1102 3 0.7138220320035309 1102 4 0.7883932160920111 1102 5 0.6666343284091596 1102 6 0.859041072027118 1102 7 0.8185749968401836 1102 8 0.7744557132507524 1102 9 0.8961853311603676 1102 10-11 0.7384719369029611 1102 12-13 0.7437605330879649 1102 14-15 0.8651274938646125 1102 16-17 0.9376949280703215 1102 18-19 0.8764501530677364 1102 20-21 0.9482840843365352 1102 22-23 1.0005900332556408 1102 24-25 0.8234440218551384 1102 26-27 0.690124786568397 1102 28-29 0.8321539200369799 1102 30-31 0.9012505532414465 1102 32-33 0.9567865763044949 1102 34-35 0.8033544676119106 1102 36-37 0.8585712102818022 1102 38-39 0.8004364170762557 1102 40-41 0.7980397655353997 1102 42-43 0.8165666355789867 1102 44-45 0.7746126232270782 1102 46-47 0.5909085534385383 1102 48-49 0.6883967299595142 1102 50-51 0.5580993486549559 1102 52-53 0.7322310332268387 1102 54-55 0.6753013120773872 1102 56-57 0.7431694772398671 1102 58-59 0.6292095467886654 1102 60-61 0.5560409245639235 1102 62-63 0.5929460176944801 1102 64-65 0.5312001344740445 1102 66-67 0.5364331237392648 1102 68-69 0.46759332907959816 1102 70-71 0.6149218949238069 1102 72-73 0.45984892753554973 1102 74-75 0.49725450062026155 1102 76-77 0.12760055130970827 1102 78-79 0.08633014527264393 1102 80-81 -0.13797586668525952 1102 82-83 -0.26167228579774715 1102 84-85 -0.47023984158716914 1102 86-87 -0.7882120819841241 1102 88-89 -1.3765035142968927 1102 90-91 -5.704352797580118 1102 92-93 -10.954327320929796 1102 94-95 -9.566496040928408 1102 96-97 -6.115776765926576 1102 98-99 -4.578548087140248 1102 100-101 -3.898445583656681 1103 1 0.7080203268239238 1103 2 0.6923501428946004 1103 3 0.6836622910527232 1103 4 0.5985819824020879 1103 5 0.6927401502688681 1103 6 0.5625234564432944 1103 7 0.6428711665929612 1103 8 0.5777620833593318 1103 9 0.7150752073023341 1103 10-11 0.5514719118012934 1103 12-13 0.5676480441545095 1103 14-15 0.7166541202345016 1103 16-17 0.8563116767209351 1103 18-19 0.7289361615592256 1103 20-21 0.7589796906726249 1103 22-23 0.6756839019155407 1103 24-25 0.5879308877198497 1103 26-27 0.677350263798246 1103 28-29 0.8167149711167312 1103 30-31 0.7663465439712809 1103 32-33 0.8342211931400243 1103 34-35 0.772704354040215 1103 36-37 0.8184256348599277 1103 38-39 0.7634240547726989 1103 40-41 0.731450341383642 1103 42-43 0.68453382965955 1103 44-45 0.5627594562246188 1103 46-47 0.646409628092492 1103 48-49 0.5003948635349147 1103 50-51 0.4400908970485631 1103 52-53 0.6000113000059244 1103 54-55 0.7209461996958098 1103 56-57 0.5560282775190757 1103 58-59 0.45698697049250825 1103 60-61 0.3101801181096988 1103 62-63 0.4720583960737912 1103 64-65 0.36685872475379 1103 66-67 0.24955886510629455 1103 68-69 0.3638672359687938 1103 70-71 0.4213900513239217 1103 72-73 0.5700587721241916 1103 74-75 0.4073618734424933 1103 76-77 0.17148649752769884 1103 78-79 0.031620218168949776 1103 80-81 -0.04696697197215016 1103 82-83 -0.06619711036647402 1103 84-85 -0.2884699088614191 1103 86-87 -0.2976981529627345 1103 88-89 -0.6691874734598642 1103 90-91 -2.360549962800885 1103 92-93 -9.481683463903902 1103 94-95 -5.746040871169043 1103 96-97 -3.2068018610058147 1103 98-99 -2.189532344835019 1103 100-101 -1.8896928907908794 1104 1 0.7376535407244091 1104 2 0.6301797044548039 1104 3 0.6967769160848221 1104 4 0.6009909229325174 1104 5 0.5205199088279073 1104 6 0.49271549105823453 1104 7 0.6926598189850779 1104 8 0.5086266320274291 1104 9 0.6938009404155281 1104 10-11 0.6220260131979671 1104 12-13 0.539830024714405 1104 14-15 0.6848036458311242 1104 16-17 0.7257965844448293 1104 18-19 0.6258083251418043 1104 20-21 0.6997201743208166 1104 22-23 0.7030466296421523 1104 24-25 0.5654853555735713 1104 26-27 0.5610440216928865 1104 28-29 0.7001940984651398 1104 30-31 0.7069509021207736 1104 32-33 0.6153215123738462 1104 34-35 0.5782768752001459 1104 36-37 0.53521600895413 1104 38-39 0.4497072547075476 1104 40-41 0.4341634457767256 1104 42-43 0.5563993935594311 1104 44-45 0.4607152148365472 1104 46-47 0.30671287844186423 1104 48-49 0.38468833989318085 1104 50-51 0.2861809249843432 1104 52-53 0.33708737838356484 1104 54-55 0.3368448165495508 1104 56-57 0.4543812942088792 1104 58-59 0.27108446369486927 1104 60-61 0.3140557507890094 1104 62-63 0.1979433357706455 1104 64-65 0.21856460563075686 1104 66-67 0.14984870273682205 1104 68-69 0.21353949805455485 1104 70-71 0.08551127633922206 1104 72-73 0.08620880045362256 1104 74-75 -0.034773859005092334 1104 76-77 -0.13819758463861476 1104 78-79 -0.010332578736765186 1104 80-81 0.05988478330305114 1104 82-83 -0.035058890848937097 1104 84-85 -0.05051990778713744 1104 86-87 -0.11102975242382129 1104 88-89 -0.18963875202537395 1104 90-91 -0.7169715930524774 1104 92-93 -4.392515853298555 1104 94-95 -1.9438198018566766 1104 96-97 -1.2351498834223484 1104 98-99 -0.9169289386625259 1104 100-101 -0.8394352364736193 1105 1 0.6426271166972413 1105 2 0.6803852044491911 1105 3 0.6235846200677884 1105 4 0.5699674441070712 1105 5 0.40175992608912026 1105 6 0.4773747868360019 1105 7 0.6126850982288374 1105 8 0.5737270530347942 1105 9 0.7612335354166291 1105 10-11 0.5035167456076479 1105 12-13 0.5357880295100585 1105 14-15 0.5869368701143713 1105 16-17 0.8138591620967688 1105 18-19 0.7265038530871095 1105 20-21 0.5341531820504457 1105 22-23 0.4648581409776327 1105 24-25 0.48661599522395704 1105 26-27 0.44293429109453797 1105 28-29 0.4823026955004721 1105 30-31 0.6305414614634657 1105 32-33 0.6536807784630554 1105 34-35 0.5950298327594652 1105 36-37 0.670471486704848 1105 38-39 0.6163648237576993 1105 40-41 0.4998587750907362 1105 42-43 0.58902003477764 1105 44-45 0.6313857425316698 1105 46-47 0.6716604291663728 1105 48-49 0.628989668721367 1105 50-51 0.4469138987192167 1105 52-53 0.4891799666463612 1105 54-55 0.7172244267288121 1105 56-57 0.6801889123709941 1105 58-59 0.5801356520407026 1105 60-61 0.5838014451145597 1105 62-63 0.5858945889218319 1105 64-65 0.6363880342373633 1105 66-67 0.5572157858013966 1105 68-69 0.43734336617914593 1105 70-71 0.538430807617889 1105 72-73 0.6144659973506066 1105 74-75 0.40530673677961815 1105 76-77 -0.0013121930756803124 1105 78-79 -0.06421980832148577 1105 80-81 0.05716459596493095 1105 82-83 0.11670974645244314 1105 84-85 -0.025218223242138293 1105 86-87 -0.5037711281108059 1105 88-89 -0.7822411161319991 1105 90-91 -1.9600403464697713 1105 92-93 -7.926365582508 1105 94-95 -3.639653199631457 1105 96-97 -1.5052653958592437 1105 98-99 -0.8988630434130904 1105 100-101 -0.7085531346088203 1106 1 0.6864763621818035 1106 2 0.6872874004976879 1106 3 0.7088726577128703 1106 4 0.7561679190252946 1106 5 0.46423575111224125 1106 6 0.6021189483389335 1106 7 0.7221955021142534 1106 8 0.6224266634174214 1106 9 0.737768118121302 1106 10-11 0.6150123793826836 1106 12-13 0.6883068379622728 1106 14-15 0.7120454173615585 1106 16-17 0.9194285644529216 1106 18-19 0.7907580598532888 1106 20-21 0.6817908865611457 1106 22-23 0.6753418198263503 1106 24-25 0.6392917008771235 1106 26-27 0.6884201344842396 1106 28-29 0.5964822320069345 1106 30-31 0.6456368933854506 1106 32-33 0.712659396896747 1106 34-35 0.6805599403717366 1106 36-37 0.8024956783320434 1106 38-39 0.5299656977711678 1106 40-41 0.6632784168271826 1106 42-43 0.6223859856515261 1106 44-45 0.4904830905206907 1106 46-47 0.6856146741002007 1106 48-49 0.6722401432553582 1106 50-51 0.6501937546555681 1106 52-53 0.6010957006819098 1106 54-55 0.6888394816876655 1106 56-57 0.6068628454059919 1106 58-59 0.5933880221490355 1106 60-61 0.6482842735164098 1106 62-63 0.6787982245821951 1106 64-65 0.5513900963148615 1106 66-67 0.46813980144756684 1106 68-69 0.4747924866582629 1106 70-71 0.41807144258406126 1106 72-73 0.5809149718192685 1106 74-75 0.49245704814051194 1106 76-77 0.5015061929708402 1106 78-79 0.3062913307393842 1106 80-81 0.26069228543911294 1106 82-83 0.27371581485193985 1106 84-85 0.21810820234503936 1106 86-87 0.25013849397827315 1106 88-89 0.13193931692989125 1106 90-91 -0.2977452414062789 1106 92-93 -4.335429886025315 1106 94-95 -1.8666123653738182 1106 96-97 -0.8031119977860683 1106 98-99 -0.4635604047223687 1106 100-101 -0.18221169556050043 1107 1 0.5841474134456632 1107 2 0.5051341031140417 1107 3 0.5455329477783195 1107 4 0.5837069947038174 1107 5 0.43822924807317065 1107 6 0.4384097724158593 1107 7 0.3461633771742001 1107 8 0.42171508578306316 1107 9 0.3786748963450606 1107 10-11 0.2641060388284089 1107 12-13 0.422547909960727 1107 14-15 0.4724320436410565 1107 16-17 0.5833147229731637 1107 18-19 0.3794458537984937 1107 20-21 0.372981694043105 1107 22-23 0.4903689279799508 1107 24-25 0.6049866197167901 1107 26-27 0.5038674965226093 1107 28-29 0.43167322914566597 1107 30-31 0.3837001876871753 1107 32-33 0.26662887674480373 1107 34-35 0.2936081441944083 1107 36-37 0.3115413947331618 1107 38-39 0.36374302247998 1107 40-41 0.5160797750614776 1107 42-43 0.46845197526781845 1107 44-45 0.28806596961997144 1107 46-47 0.41843094956937676 1107 48-49 0.44823643400648905 1107 50-51 0.511542524348414 1107 52-53 0.6316809559617553 1107 54-55 0.5187493582466303 1107 56-57 0.4022284726684262 1107 58-59 0.3567130519196766 1107 60-61 0.3740790539430989 1107 62-63 0.17279066384187658 1107 64-65 0.32461156254410284 1107 66-67 0.19621328980100472 1107 68-69 0.16413341112562918 1107 70-71 0.09778865338939013 1107 72-73 0.22966442245288832 1107 74-75 0.31484712471443643 1107 76-77 0.001564366539614781 1107 78-79 0.02258403314784374 1107 80-81 -0.08298667305340857 1107 82-83 -0.020045206668633853 1107 84-85 -0.150047421981359 1107 86-87 -0.14899893241575413 1107 88-89 -0.23614463901867566 1107 90-91 -0.39867045645700827 1107 92-93 -4.433465479496014 1107 94-95 -1.88755485013289 1107 96-97 -0.8488089773195497 1107 98-99 -0.571947428551649 1107 100-101 -0.45945652969288275 1108 1 0.3346586703941128 1108 2 0.2892694901510424 1108 3 0.10165284837757582 1108 4 -0.16053206904527428 1108 5 -0.9623432047827407 1108 6 -0.13218427717296066 1108 7 0.014604260378327183 1108 8 0.10268842605709239 1108 9 0.20336643798616194 1108 10-11 0.2516291228542613 1108 12-13 0.16960911206524543 1108 14-15 0.3265894921900099 1108 16-17 0.4350569472197563 1108 18-19 0.34184096866088964 1108 20-21 0.3876030230632921 1108 22-23 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0.0540890711976445 2311 64-65 0.24958254116348044 2311 66-67 0.198009621129966 2311 68-69 0.2917206902406768 2311 70-71 0.3532131947287347 2311 72-73 0.2990480175416721 2311 74-75 0.42947543606932115 2311 76-77 0.4355143914150865 2311 78-79 0.5084801795998644 2311 80-81 0.6248969312485144 2311 82-83 0.7064904696677345 2311 84-85 0.5595624938105708 2311 86-87 0.7919438632184495 2311 88-89 0.9774383652009391 2311 90-91 1.8747585501825945 2311 92-93 3.1487613049350145 2311 94-95 2.210835307780272 2311 96-97 1.72242484594792 2311 98-99 1.767449172415116 2311 100-101 1.9055310172173385 2312 1 0.29366316043484986 2312 2 0.2059110071654544 2312 3 0.09736747377547417 2312 4 0.1568580403769957 2312 5 0.23541355661060237 2312 6 0.21654048566345097 2312 7 0.32895504615122206 2312 8 -0.4310675318088002 2312 9 0.12256872570575439 2312 10-11 0.019173291635389944 2312 12-13 0.1764030659311686 2312 14-15 -0.11871852876728894 2312 16-17 0.21659472940830682 2312 18-19 0.20747012150707178 2312 20-21 0.24916546137174578 2312 22-23 0.1124055755468163 2312 24-25 0.19359335460335814 2312 26-27 0.20340293945085364 2312 28-29 0.22255011234530997 2312 30-31 0.33727595340389627 2312 32-33 0.2654213485539145 2312 34-35 0.18343193504571786 2312 36-37 0.42234206355164616 2312 38-39 0.4372627617626037 2312 40-41 0.5460962456147982 2312 42-43 0.5526697255104978 2312 44-45 0.30012231312588966 2312 46-47 0.4352418474683404 2312 48-49 0.3375339324972728 2312 50-51 0.25282320553738913 2312 52-53 0.38019450609619554 2312 54-55 0.512256492753032 2312 56-57 0.4652842171741369 2312 58-59 0.4632019735396753 2312 60-61 0.45712137365926253 2312 62-63 0.48490698546515887 2312 64-65 0.4570619888338854 2312 66-67 0.4613406222508054 2312 68-69 0.24893731470143265 2312 70-71 0.3355980619879766 2312 72-73 0.4328176079703603 2312 74-75 0.5456722845975115 2312 76-77 0.8116963730653595 2312 78-79 0.8939031300085993 2312 80-81 0.8605254205811796 2312 82-83 0.9466118801598462 2312 84-85 1.0716430628183282 2312 86-87 0.9794985610768556 2312 88-89 1.2589598423931818 2312 90-91 1.6202902039895335 2312 92-93 2.7017864246920666 2312 94-95 2.197443240407516 2312 96-97 2.064107989693266 2312 98-99 1.9831279853403139 2312 100-101 1.9060818637213934 2313 1 -0.01802702038516557 2313 2 -0.6059891799267412 2313 3 -0.1262016607390848 2313 4 0.1377910647490097 2313 5 -0.5751352205910862 2313 6 -0.17826957011386924 2313 7 -0.8926888796333543 2313 8 -0.5772646429147059 2313 9 -0.8330635730774247 2313 10-11 -1.7091356406497908 2313 12-13 -0.2913548758606055 2313 14-15 0.08010462338464208 2313 16-17 -1.0939648972626372 2313 18-19 -0.8266964352840631 2313 20-21 -1.1033310330661052 2313 22-23 -0.8118782273160363 2313 24-25 -0.17636443346523833 2313 26-27 -0.7180000728825817 2313 28-29 -2.149826949556868 2313 30-31 -0.4702159562455037 2313 32-33 -0.078583562192037 2313 34-35 0.23122041473996546 2313 36-37 0.17498312026312846 2313 38-39 -0.0024751434660643667 2313 40-41 0.08423126209139298 2313 42-43 0.2826544076380131 2313 44-45 -0.31979651548492427 2313 46-47 -0.4903658668120343 2313 48-49 -0.06694793196390236 2313 50-51 0.07775088297533017 2313 52-53 0.17931630665675158 2313 54-55 0.2426903034551131 2313 56-57 -0.07080395815896168 2313 58-59 0.003854974293773239 2313 60-61 -0.026576253289587015 2313 62-63 0.16947582874051292 2313 64-65 0.2665267265201514 2313 66-67 0.24033630226831448 2313 68-69 0.1383695450106508 2313 70-71 -0.007283474984927096 2313 72-73 0.08222983106921689 2313 74-75 0.20757327257779323 2313 76-77 0.3768921752123866 2313 78-79 0.4881860436094989 2313 80-81 0.5789402286943108 2313 82-83 0.4495331096201234 2313 84-85 0.6846272657543757 2313 86-87 0.6129097502015703 2313 88-89 0.6273421691679921 2313 90-91 1.4035515796975027 2313 92-93 2.725497130972002 2313 94-95 1.867299966656308 2313 96-97 1.5120824963600086 2313 98-99 1.516715969525496 2313 100-101 1.219717492463129 2314 1 -0.26967506447653733 2314 2 -0.3778823674370564 2314 3 -1.485735055788851 2314 4 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64-65 -0.5653415591264697 2314 66-67 -0.46388409716618995 2314 68-69 -0.34462865373062 2314 70-71 -0.502756287460258 2314 72-73 -0.13264311201531243 2314 74-75 -0.055110889980422684 2314 76-77 -0.205391619917755 2314 78-79 -0.1349188671528445 2314 80-81 -0.17794330665270053 2314 82-83 -0.11405204111867917 2314 84-85 -0.20100787529637998 2314 86-87 -0.0029646496778674702 2314 88-89 0.21125534993256068 2314 90-91 0.6819827622848571 2314 92-93 1.6512102787254506 2314 94-95 0.7651757730963489 2314 96-97 0.7732940760854419 2314 98-99 0.9165559373755379 2314 100-101 0.8656869069463973 2315 1 -7.281893401694873 2315 2 -2.893843258397947 2315 3 -1.2130207330745293 2315 4 -0.8311000357541829 2315 5 -1.1757120507019252 2315 6 -1.54733931229444 2315 7 -2.839825426266124 2315 8 -2.594185449508057 2315 9 -3.1937467955364696 2315 10-11 -2.102100480875336 2315 12-13 -2.6757194966861846 2315 14-15 -4.443847844522491 2315 16-17 -4.263620912951396 2315 18-19 -3.057878998173173 2315 20-21 -4.1574594368481605 2315 22-23 -5.554411337922197 2315 24-25 -3.427592931387693 2315 26-27 -4.400860208828366 2315 28-29 -2.6900163116920695 2315 30-31 -2.6588645767266215 2315 32-33 -1.689153268164759 2315 34-35 -2.3668980739641654 2315 36-37 -2.8257297828427212 2315 38-39 -1.8975934941889463 2315 40-41 -3.261932125318843 2315 42-43 -7.488689382912327 2315 44-45 -4.012534053625487 2315 46-47 -2.587025032583284 2315 48-49 -5.347841541572873 2315 50-51 -6.417896469252629 2315 52-53 -6.65415586418947 2315 54-55 -4.251181518817205 2315 56-57 -3.4618669079435804 2315 58-59 -3.845679534718066 2315 60-61 -2.986268481716074 2315 62-63 -3.108096812024826 2315 64-65 -1.8077366640215793 2315 66-67 -2.4343742551563494 2315 68-69 -1.88764212174409 2315 70-71 -1.5842086314126007 2315 72-73 -2.11520591957812 2315 74-75 -2.6766953240648554 2315 76-77 -2.581314308340442 2315 78-79 -1.726954609188585 2315 80-81 -1.3698689735783667 2315 82-83 -1.7724274619940985 2315 84-85 -1.711014997803506 2315 86-87 -2.129907151620369 2315 88-89 -1.6947502185730094 2315 90-91 -0.7578385275364319 2315 92-93 0.331833821848992 2315 94-95 0.08600586892644202 2315 96-97 -0.2532275504361863 2315 98-99 -0.3276813068617024 2315 100-101 -0.36712518836569785 2316 1 -4.045472876504906 2316 2 -2.6827230656903787 2316 3 -4.221095534150244 2316 4 -4.0129422100483225 2316 5 -3.595182904642126 2316 6 -3.8822948035857046 2316 7 -2.852452932946697 2316 8 -1.3428008170621268 2316 9 -1.7842146914709645 2316 10-11 -2.7852533108145323 2316 12-13 -3.5414029516880277 2316 14-15 -2.7223135843331505 2316 16-17 -2.6566813825214766 2316 18-19 -2.9217242296419172 2316 20-21 -5.321799499804417 2316 22-23 -5.965023103405876 2316 24-25 -3.711956421663807 2316 26-27 -3.4468967051164654 2316 28-29 -4.0172173014616845 2316 30-31 -5.752313004632963 2316 32-33 -3.7912948365689125 2316 34-35 -5.553689229591093 2316 36-37 -8.838771559608197 2316 38-39 -5.588493361323948 2316 40-41 -5.026964477839762 2316 42-43 -3.987985870200575 2316 44-45 -2.7937154751317017 2316 46-47 -5.262782581682554 2316 48-49 -2.799606001571547 2316 50-51 -3.7866476288889643 2316 52-53 -4.665026455172139 2316 54-55 -6.206878345625654 2316 56-57 -4.285756936030772 2316 58-59 -5.291376697421171 2316 60-61 -5.64171933232932 2316 62-63 -5.899585001328656 2316 64-65 -4.621448017474467 2316 66-67 -5.962559949455947 2316 68-69 -4.503699326204771 2316 70-71 -5.581039092046812 2316 72-73 -7.5623118404946545 2316 74-75 -7.404635708626923 2316 76-77 -6.524594628965229 2316 78-79 -5.457211467304546 2316 80-81 -5.5750454302415555 2316 82-83 -4.535175064346003 2316 84-85 -6.054633772289161 2316 86-87 -4.486670204060456 2316 88-89 -4.389228563497376 2316 90-91 -5.244500843567462 2316 92-93 -3.8950127723923877 2316 94-95 -3.785732753841458 2316 96-97 -4.582668237836614 2316 98-99 -5.61857568238554 2316 100-101 -4.830659957195525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13340.0 3 5038.0 4 5166.0 5 6026.0 6 6782.0 7 7802.0 8 9594.0 9 11623.0 10 14250.0 11 17142.0 12 20953.0 13 24286.0 14 26469.0 15 30515.0 16 35574.0 17 40083.0 18 37596.0 19 35711.0 20 38125.0 21 42391.0 22 47221.0 23 53596.0 24 61267.0 25 70135.0 26 81104.0 27 93614.0 28 108914.0 29 127482.0 30 149002.0 31 173959.0 32 206410.0 33 243260.0 34 292600.0 35 355008.0 36 411653.0 37 358475.0 38 110006.0 39 1881.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.60379579226129 19.11914411955939 13.500619343448486 23.776440744730834 2 15.43774017225657 20.421664909531778 40.41417162412066 23.726423294090985 3 18.86422889369373 25.105179647104226 29.95923927149801 26.07135218770403 4 12.5630631632812 16.805600670419235 37.78580698748897 32.8455291788106 5 13.542436989621645 37.52068655482949 34.8454578273118 14.091418628237061 6 31.504032848869457 36.67564173519573 17.987923698273974 13.832401717660836 7 27.64342100735541 32.73299943876285 21.96780658320489 17.655772970676843 8 25.738648565594435 35.20546425524695 20.42988266812842 18.626004511030192 9 26.56139235212428 16.233805909986398 20.437640110504514 36.76716162738481 10-11 24.60881227809534 26.32349318403513 28.37082621985178 20.69686831801775 12-13 25.658385336362056 23.854647999957287 28.42187740079578 22.065089262884875 14-15 22.811256142715685 25.12445618092319 26.422917854470963 25.64136982189017 16-17 22.415530200950087 28.17117428849798 26.91902387916565 22.494271631386283 18-19 22.193115176835683 27.13050693078764 28.948632656908472 21.727745235468202 20-21 22.955293021724184 26.45430252471051 28.869379736010853 21.721024717554453 22-23 22.622325721875967 26.318465955533455 28.737847831575774 22.321360491014804 24-25 22.51533219812055 26.52127514982019 28.80442685504314 22.15896579701612 26-27 22.403598742843915 26.72241661250449 28.6962792809637 22.177705363687895 28-29 22.231966342128118 26.876231524443817 28.84757579898939 22.044226334438672 30-31 22.460221407996258 26.58360552789918 28.76674642071318 22.189426643391386 32-33 22.23101804650892 26.71247156051162 28.66865134096092 22.387859052018538 34-35 22.606056865163353 26.80795810784985 28.524456673341653 22.061528353645148 36-37 22.602684945971223 26.605650248342588 28.49709887363322 22.29456593205297 38-39 22.3785855547067 26.81958643500158 28.658101509736138 22.14372650055558 40-41 22.55321661686024 26.65360453429878 28.665772317121984 22.127406531718993 42-43 22.39183413801279 26.757889058387057 28.42471942052397 22.425557383076182 44-45 22.469250124503784 26.85047781711229 28.320757350915528 22.359514707468403 46-47 22.51364049397195 26.8889257528609 28.064058834107968 22.53337491905918 48-49 22.50989121885342 27.082498553047508 27.947345769704523 22.460264458394548 50-51 22.420641870411256 27.141076963538215 27.93482152027314 22.50345964577739 52-53 22.605079922324148 27.213354333064828 27.6981530537258 22.48341269088523 54-55 22.466089228568446 26.967191349975906 27.819174018224373 22.747545403231275 56-57 22.422033687803562 27.387436152995697 27.73178671602452 22.45874344317622 58-59 22.51868597006209 27.206247173513344 27.773235945907125 22.50183091051744 60-61 22.559339430354523 27.12109662109974 27.87421355281106 22.445350395734675 62-63 22.43615135851109 27.067735209544132 27.97998158564618 22.5161318462986 64-65 22.359436723347383 27.098944264211344 27.899505582775948 22.64211342966533 66-67 22.335912227781442 27.31314191713316 27.598883501108844 22.752062353976555 68-69 22.288299101026414 27.209769652390452 27.786183890874 22.71574735570914 70-71 22.24757836926295 27.133391879475067 27.76334895800182 22.85568079326016 72-73 22.453604798829588 27.004903279731522 27.622848016137386 22.918643905301504 74-75 22.286331490948427 27.271947352062746 27.50943184506749 22.932289311921338 76-77 22.357307778799505 27.116839704525702 27.56774007286664 22.958112443808155 78-79 22.281906390406597 27.277000740671475 27.439592229925797 23.00150063899613 80-81 22.26439746665507 27.227698522096706 27.471749546440055 23.036154464808174 82-83 22.308477441415906 27.204193847278336 27.495908249793743 22.991420461512018 84-85 22.3288490505836 27.112290100692306 27.349262059703044 23.20959878902105 86-87 22.217097259960696 27.024999476867993 27.64765271039516 23.110250552776154 88-89 22.24459811854452 27.001050332852795 27.43627941110111 23.318072137501574 90-91 21.956377620835763 27.09943954326684 27.7184647627798 23.225718073117598 92-93 21.76580432176178 26.954490103120527 28.091153590743033 23.18855198437466 94-95 22.204251564664382 26.873619173662455 27.4534845956038 23.46864466606936 96-97 22.241300118198502 26.833123370910815 27.34804841833422 23.577528092556463 98-99 22.12528687999437 26.71671794883978 27.272623337424918 23.885371833740933 100-101 22.195715185136788 26.790576258300568 27.34322471816681 23.670483838395835 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3464.0 1 2155.0 2 916.0 3 972.0 4 880.5 5 894.5 6 1251.0 7 2332.0 8 3705.5 9 4193.5 10 3692.5 11 3145.5 12 3103.0 13 3279.0 14 3486.5 15 3718.0 16 3996.0 17 4212.0 18 4478.0 19 4937.5 20 5550.5 21 6513.0 22 7816.0 23 9616.0 24 11751.0 25 14639.5 26 18942.0 27 24068.0 28 28687.0 29 33439.0 30 38981.5 31 44894.5 32 50805.5 33 56011.5 34 62378.5 35 69105.0 36 76441.5 37 83842.5 38 89221.5 39 92233.0 40 94530.0 41 99546.5 42 104400.0 43 108611.0 44 114486.0 45 119869.5 46 122901.0 47 126048.5 48 130941.0 49 132756.0 50 130669.5 51 128605.0 52 126011.5 53 123021.5 54 119062.0 55 112349.5 56 102945.5 57 91849.0 58 81501.0 59 71380.5 60 60265.0 61 48255.5 62 37446.5 63 29012.5 64 21975.0 65 16407.5 66 11847.0 67 8567.5 68 6000.5 69 3745.5 70 2405.5 71 1602.0 72 1033.5 73 642.5 74 390.5 75 266.5 76 178.5 77 120.0 78 87.0 79 59.5 80 50.0 81 43.5 82 31.0 83 23.0 84 17.5 85 9.5 86 7.5 87 8.0 88 8.0 89 9.5 90 6.0 91 5.5 92 6.0 93 5.5 94 5.0 95 2.0 96 2.0 97 2.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08123760948627659 2 0.0899511655566762 3 0.0864242500043716 4 0.0897436999359524 5 0.086009318762924 6 0.07382812303185515 7 0.08680954330000151 8 0.0818303684026303 9 0.0772661247467067 10-11 0.08338636055805881 12-13 0.08224529964407791 14-15 0.0885285441574273 16-17 0.08572775827765598 18-19 0.08806915599725315 20-21 0.09693090179674119 22-23 0.10562963889423195 24-25 0.12883615046947988 26-27 0.13464518784974627 28-29 0.13335593720667696 30-31 0.1341709807166633 32-33 0.1303625046790907 34-35 0.12616873534588816 36-37 0.13080707386635598 38-39 0.12327903562866381 40-41 0.11872961094564906 42-43 0.1177811966794831 44-45 0.10887499396126854 46-47 0.12765063263677245 48-49 0.14531484834411315 50-51 0.15444333565596036 52-53 0.16118596832948387 54-55 0.1577479666146323 56-57 0.1297104698711016 58-59 0.12284928541430737 60-61 0.11831467970420144 62-63 0.11428391907299619 64-65 0.10130249880484984 66-67 0.11484704004353222 68-69 0.11323177199646835 70-71 0.12037451693853062 72-73 0.1476414270908015 74-75 0.16040056276531517 76-77 0.16509817717741837 78-79 0.1626530466474593 80-81 0.1689362911608087 82-83 0.16863991170263182 84-85 0.144573899698671 86-87 0.14589278828755803 88-89 0.16588358274158704 90-91 0.2596876812545624 92-93 0.5400774676627783 94-95 0.16594285863322242 96-97 0.17753129544793753 98-99 0.19482503683255714 100-101 0.20192332485589293 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3374053.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.67939864149221 #Duplication Level Percentage of deduplicated Percentage of total 1 72.05705968538298 37.238655124147044 2 13.910522950407058 14.377749217314257 3 5.538800961640219 8.58725708677456 4 2.777323147403098 5.741215603635542 5 1.5996239524053613 4.133380195641802 6 0.9600055673783806 2.9767506248759545 7 0.669326781967161 2.4213283910695638 8 0.4617824089043355 1.909170975631657 9 0.3553619230776784 1.6528401436264768 >10 1.560422954213069 13.620806563630458 >50 0.07066908272900288 2.489328813788114 >100 0.03712951667441942 3.3403707658983794 >500 0.001391341991156784 0.45607928705295003 >1k 4.0580807824825533E-4 0.26364696220002737 >5k 1.1594516521378722E-4 0.42671860029715797 >10k+ 5.797258260689361E-5 0.3647016444160151 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12173 0.3607827144386884 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8155 0.24169744814322713 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6088 0.18043581413807072 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.89138374530572E-5 0.0 5 0.0 0.0 0.0 8.89138374530572E-5 0.0 6 0.0 0.0 0.0 1.778276749061144E-4 0.0 7 0.0 0.0 0.0 1.778276749061144E-4 0.0 8 0.0 0.0 0.0 1.778276749061144E-4 0.0 9 0.0 0.0 0.0 2.3710356654148585E-4 2.9637945817685732E-5 10-11 0.0 0.0 0.0 3.4083637690338596E-4 2.9637945817685732E-5 12-13 0.0 0.0 0.0 4.44569187265286E-4 2.9637945817685732E-5 14-15 0.0 0.0 0.0 4.5938816017412887E-4 4.44569187265286E-5 16-17 0.0 0.0 0.0 7.854055641686719E-4 8.89138374530572E-5 18-19 0.0 0.0 0.0 9.78052211983629E-4 8.89138374530572E-5 20-21 0.0 0.0 0.0 0.0010521470765278435 8.89138374530572E-5 22-23 0.0 0.0 0.0 0.0013188885888870152 1.0373281036190006E-4 24-25 0.0 0.0 0.0 0.0017634577761523012 1.1855178327074293E-4 26-27 0.0 0.0 0.0 0.0021783890175999013 1.4818972908842867E-4 28-29 0.0 0.0 0.0 0.0037047432272107163 1.4818972908842867E-4 30-31 0.0 0.0 0.0 0.008046702289501677 1.4818972908842867E-4 32-33 0.0 0.0 0.0 0.01711591370971351 1.4818972908842867E-4 34-35 0.0 1.4818972908842866E-5 0.0 0.030052877059133332 1.4818972908842867E-4 36-37 0.0 2.9637945817685732E-5 0.0 0.05094762886060177 1.4818972908842867E-4 38-39 0.0 2.9637945817685732E-5 0.0 0.08365310207041798 1.4818972908842867E-4 40-41 0.0 2.9637945817685732E-5 0.0 0.11856660224365177 1.4818972908842867E-4 42-43 0.0 2.9637945817685732E-5 0.0 0.1565328108361072 1.6300870199727153E-4 44-45 0.0 2.9637945817685732E-5 0.0 0.20282728220333232 2.22284593632643E-4 46-47 0.0 2.9637945817685732E-5 0.0 0.25826505985531345 3.111984310857002E-4 48-49 0.0 2.9637945817685732E-5 0.0 0.3172297530595992 3.556553498122288E-4 50-51 0.0 2.9637945817685732E-5 0.0 0.38274443228959354 3.556553498122288E-4 52-53 0.0 2.9637945817685732E-5 0.0 0.45111917329099455 3.556553498122288E-4 54-55 0.0 2.9637945817685732E-5 0.0 0.5282963842002482 3.7047432272107163E-4 56-57 0.0 2.9637945817685732E-5 0.0 0.6193293347792699 3.852932956299145E-4 58-59 0.0 2.9637945817685732E-5 0.0 0.7212097735275647 3.852932956299145E-4 60-61 0.0 2.9637945817685732E-5 0.0 0.8339377004451323 3.852932956299145E-4 62-63 0.0 2.9637945817685732E-5 0.0 0.9496738788631951 5.334830247183432E-4 64-65 0.0 2.9637945817685732E-5 0.0 1.0749831137803705 5.483019976271861E-4 66-67 0.0 2.9637945817685732E-5 0.0 1.2182974007817897 5.631209705360289E-4 68-69 0.0 2.9637945817685732E-5 0.0 1.3836475004986584 5.631209705360289E-4 70-71 0.0 2.9637945817685732E-5 0.0 1.5668989194894094 5.927589163537147E-4 72-73 0.0 2.9637945817685732E-5 0.0 1.7611608353514305 6.223968621714003E-4 74-75 0.0 2.9637945817685732E-5 0.0 1.9640029365276717 6.223968621714003E-4 76-77 0.0 2.9637945817685732E-5 0.0 2.1845833482757975 6.372158350802432E-4 78-79 0.0 2.9637945817685732E-5 0.0 2.437172741507024 6.66853780897929E-4 80-81 0.0 2.9637945817685732E-5 0.0 2.7097973861110063 7.261296725333004E-4 82-83 0.0 2.9637945817685732E-5 0.0 2.993891915746433 7.854055641686719E-4 84-85 0.0 2.9637945817685732E-5 0.0 3.276326720416069 8.002245370775148E-4 86-87 0.0 2.9637945817685732E-5 0.0 3.5801008460744392 8.002245370775148E-4 88-89 1.4818972908842866E-5 2.9637945817685732E-5 0.0 3.9095117948651072 8.002245370775148E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11045 0.0 39.99989 1 GGTATCA 6370 0.0 39.855583 1 TCAACGC 13640 0.0 32.18215 4 ATCAACG 13730 0.0 32.005287 3 TATCAAC 13845 0.0 31.807993 2 CAACGCA 14010 0.0 31.297892 5 AACGCAG 14495 0.0 30.47487 6 ACGCAGA 16180 0.0 27.007944 7 CGCAGAG 16085 0.0 26.638075 8 GTACATG 9880 0.0 24.303474 1 TACATGG 9890 0.0 23.8954 2 GCAGAGT 17130 0.0 23.711145 9 ACATGGG 10400 0.0 21.537233 3 AGAGTAC 14090 0.0 21.097929 10-11 GAGTACT 9165 0.0 21.07147 12-13 AGTACTT 9910 0.0 20.397121 12-13 GTACTTT 10400 0.0 18.570612 14-15 CAGAGTA 16210 0.0 18.426483 10-11 CATGGGG 6085 0.0 17.781237 4 CATGGGA 5630 0.0 16.605253 4 >>END_MODULE