FastQCFastQC Report
Fri 27 May 2016
SRR939571_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939571_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3374053
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132180.39175436781816997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97500.2889699717224359No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77340.22921987295398144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69200.042.560291
GTATCAA115000.035.8379061
TCAACGC141600.028.865784
ATCAACG142750.028.6002853
TATCAAC145950.028.0715642
CAACGCA146150.027.9349375
AACGCAG150400.027.200626
GTACATG98700.024.9775871
TACATGG96700.024.3218212
ACGCAGA169400.023.8447937
CGCAGAG168500.023.4982858
ACATGGG97550.023.1441253
GCAGAGT180850.020.8284979
GAGTACT99450.020.3551612-13
AGAGTAC149250.019.74701310-11
AGTACTT107100.018.9889312-13
CATGGGG56800.018.5492534
GTACTTT112400.018.23979614-15
CAGAGTA173750.017.75998710-11
CATGGGA52150.016.2347264
Produced by FastQC (version 0.11.5)