FastQCFastQC Report
Fri 27 May 2016
SRR939570_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939570_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3280764
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50250.15316554314787653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53400.051.0763171
GTATCAA89900.046.407421
TATCAAC128100.032.119352
ATCAACG136400.030.5467623
TCAACGC138600.030.1959194
GTACATG83500.029.8818571
CAACGCA140550.029.6526015
AACGCAG145100.028.7552936
ACGCAGA152000.026.9216587
CGCAGAG160550.025.163438
TACATGG95350.024.3253142
ACATGGG94950.023.134973
GCAGAGT164600.022.853619
GAGTACT86300.021.6582812-13
AGAGTAC134500.020.28360210-11
GTATAGT7857.2759576E-1219.3027291
GTACTTT97400.019.0720914-15
AGTACTT97100.018.98197212-13
GAGTACA70650.018.8737771
CAGAGTA159950.018.04478610-11
Produced by FastQC (version 0.11.5)