FastQCFastQC Report
Fri 27 May 2016
SRR939570_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939570_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3280764
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125600.38283765610693116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94190.28709776137509435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75300.2295197094335344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69100.040.7575381
GTATCAA113000.036.4457971
TCAACGC143800.028.1406484
TATCAAC145700.028.0995252
ATCAACG144450.028.079323
CAACGCA147400.027.453365
AACGCAG151150.026.7542936
ACGCAGA170750.023.2691887
CGCAGAG170850.022.9521168
GTACATG98300.022.891621
TACATGG96850.022.6460322
ACATGGG98400.021.2331453
GCAGAGT182550.020.4228159
AGTACTT107650.020.1408612-13
GAGTACT100950.020.00685112-13
AGAGTAC151400.019.90867210-11
CATGGGG57850.018.0990314
GTACTTT111150.018.04364814-15
GAGTACA85000.017.537991
CAGAGTA174050.017.06059310-11
Produced by FastQC (version 0.11.5)