##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939570_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3280764 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.27177602534044 31.0 31.0 34.0 30.0 34.0 2 31.49825467482574 31.0 31.0 34.0 30.0 34.0 3 31.539429230508503 31.0 31.0 34.0 30.0 34.0 4 35.37947167184229 37.0 35.0 37.0 33.0 37.0 5 35.11653200291152 37.0 35.0 37.0 32.0 37.0 6 35.12388242494736 37.0 35.0 37.0 32.0 37.0 7 35.023439662225016 37.0 35.0 37.0 32.0 37.0 8 34.98041797581295 37.0 35.0 37.0 32.0 37.0 9 36.55331593494686 38.0 35.0 39.0 32.0 39.0 10-11 36.417004850089796 38.0 35.0 39.0 32.0 39.0 12-13 36.345900680451265 38.0 35.0 39.0 32.0 39.0 14-15 37.3678498361967 39.0 36.0 41.0 32.0 41.0 16-17 37.3074427176109 39.0 36.0 40.5 32.0 41.0 18-19 37.25330456564386 39.0 36.0 40.0 31.5 41.0 20-21 37.18826910439154 39.0 36.0 40.0 31.0 41.0 22-23 37.03754140803788 39.0 36.0 40.0 31.0 41.0 24-25 36.88979685829276 39.0 36.0 40.0 31.0 41.0 26-27 36.574188664591546 38.0 35.0 40.0 30.0 41.0 28-29 36.346533612292745 38.0 35.0 40.0 30.0 41.0 30-31 36.08410022787375 38.0 34.5 40.0 29.5 41.0 32-33 35.90103539907168 38.0 34.0 40.0 29.0 41.0 34-35 35.85886488634964 38.0 34.0 40.0 29.0 41.0 36-37 35.77386517286827 38.0 34.0 40.0 29.0 41.0 38-39 35.817817130400115 38.0 34.5 40.0 29.0 41.0 40-41 35.6900072666001 38.0 34.0 40.0 28.0 41.0 42-43 35.491602870550885 38.0 34.0 40.0 27.0 41.0 44-45 35.258979768127176 38.0 34.0 40.0 27.0 41.0 46-47 34.955706048956884 38.0 33.5 40.0 26.0 41.0 48-49 34.641031784060054 38.0 33.0 40.0 25.0 41.0 50-51 34.3759142687496 38.0 33.0 40.0 24.0 41.0 52-53 34.11120610930868 37.5 33.0 40.0 24.0 41.0 54-55 33.79259175606657 37.0 32.5 40.0 23.0 41.0 56-57 32.906953075564104 36.5 31.5 39.5 19.0 40.5 58-59 29.587484957772034 34.5 25.0 38.5 7.5 40.0 60-61 29.254393336430176 33.5 24.0 38.0 9.0 40.0 62-63 31.09909261988976 34.5 29.0 38.0 11.5 40.0 64-65 31.129736549169643 35.0 30.0 38.0 7.0 40.0 66-67 26.12674654440246 30.5 18.0 36.5 2.0 39.0 68-69 18.648883308887807 17.0 7.5 31.0 2.0 36.0 70-71 16.74487131655919 13.0 7.0 27.0 2.0 34.0 72-73 18.282094658439313 16.5 7.5 29.5 2.0 34.5 74-75 19.675183128076267 22.0 7.5 31.0 2.0 35.0 76-77 21.685275746746797 26.0 10.0 32.0 2.0 35.5 78-79 23.873597125547587 29.5 11.5 34.0 2.0 36.0 80-81 25.40322513292635 31.0 19.0 35.0 2.0 36.0 82-83 26.475380734487455 32.0 25.0 35.0 2.0 36.0 84-85 26.503093181953957 33.0 25.0 35.0 2.0 36.0 86-87 26.193166134473557 32.5 24.5 35.0 2.0 35.5 88-89 25.71235648160002 32.0 22.0 35.0 2.0 35.0 90-91 23.82626470541618 31.5 10.0 34.0 2.0 35.0 92-93 22.070774063602258 30.0 2.0 34.0 2.0 35.0 94-95 18.287280188395144 24.5 2.0 33.0 2.0 35.0 96-97 15.717369643168482 7.0 2.0 32.0 2.0 34.0 98-99 15.825709346969182 2.0 2.0 32.0 2.0 34.0 100-101 15.02256898088372 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2673763177812667 1101 2 0.25226691016505853 1101 3 0.2693012116423361 1101 4 0.21795345518695086 1101 5 0.38106703543240883 1101 6 0.3439967973450351 1101 7 0.3581068976326307 1101 8 0.4030507542971975 1101 9 0.4443432461293426 1101 10-11 0.3554829946979652 1101 12-13 0.30147003453828347 1101 14-15 0.5007426804112569 1101 16-17 0.5121272235423788 1101 18-19 0.5031740505993341 1101 20-21 0.5487358993238232 1101 22-23 0.551771265229668 1101 24-25 0.38923078113942466 1101 26-27 0.4815205005786751 1101 28-29 0.42847000538639435 1101 30-31 0.31716286055362275 1101 32-33 0.23029404985712887 1101 34-35 0.1578579170539811 1101 36-37 0.1633240710681676 1101 38-39 0.2324515162694567 1101 40-41 0.2338749881243558 1101 42-43 0.37781619647096676 1101 44-45 0.3746883532650216 1101 46-47 0.34840620011716084 1101 48-49 0.2424995495449238 1101 50-51 0.3170495861469931 1101 52-53 0.2627703317858021 1101 54-55 0.20958005969936977 1101 56-57 -3.6898624853868824 1101 58-59 -10.307654698024425 1101 60-61 -11.67810523337608 1101 62-63 -4.337937789328837 1101 64-65 -1.4870149113448825 1101 66-67 -2.216886081437565 1101 68-69 -4.359356916117719 1101 70-71 -4.4276540049918935 1101 72-73 -3.21155227114566 1101 74-75 -6.0186471187579045 1101 76-77 -4.786549603440225 1101 78-79 1.1253299150663452 1101 80-81 1.8936874237980241 1101 82-83 0.6150711073736339 1101 84-85 0.39278451905207135 1101 86-87 0.33784700090086517 1101 88-89 0.17084320855917667 1101 90-91 1.513474728172838 1101 92-93 0.5334876109818474 1101 94-95 -8.64451400157726 1101 96-97 -9.440156849909908 1101 98-99 -8.861620162203529 1101 100-101 -9.309871649160607 1102 1 0.22411973647830763 1102 2 0.291082056182411 1102 3 0.25288012098087975 1102 4 0.21438288956518647 1102 5 0.3323258232465136 1102 6 0.35673505642806447 1102 7 0.3550050308299504 1102 8 0.3065445355485963 1102 9 0.2802824894567948 1102 10-11 0.30963470989539843 1102 12-13 0.3377385220386486 1102 14-15 0.39645869466487227 1102 16-17 0.3444805397539952 1102 18-19 0.3447128472653489 1102 20-21 0.39184341638431874 1102 22-23 0.39507189821561894 1102 24-25 0.4391719223621422 1102 26-27 0.08722502027673329 1102 28-29 0.25954820209020824 1102 30-31 0.33231784070379433 1102 32-33 0.10921914259204613 1102 34-35 0.22455082531052284 1102 36-37 0.10062988676812523 1102 38-39 0.2402312467664771 1102 40-41 0.09692160898836022 1102 42-43 0.09646061507422843 1102 44-45 0.01438127584355442 1102 46-47 -0.12084228325291946 1102 48-49 0.15629119132135116 1102 50-51 0.2067045092401969 1102 52-53 0.31074312276830796 1102 54-55 -0.07489251899421134 1102 56-57 -3.7382492282096003 1102 58-59 -10.065894349343925 1102 60-61 -8.180115625600067 1102 62-63 -2.6070977304331002 1102 64-65 -0.9240095701649444 1102 66-67 -1.8924020081884159 1102 68-69 -4.813264348909449 1102 70-71 -4.591519104739964 1102 72-73 -3.411781723357091 1102 74-75 -4.027038537018818 1102 76-77 -4.284106779383155 1102 78-79 1.5137882586214246 1102 80-81 1.9207626923469725 1102 82-83 0.7433081668741259 1102 84-85 0.47175530356934914 1102 86-87 0.4154471474472068 1102 88-89 0.24583836911104484 1102 90-91 1.3384151417458376 1102 92-93 0.39669962778200585 1102 94-95 -9.01327833223423 1102 96-97 -9.565648359970183 1102 98-99 -8.774819289208667 1102 100-101 -9.183326317507106 1103 1 0.2629995624706929 1103 2 0.2570961943009529 1103 3 0.285506548387076 1103 4 0.2872485774390299 1103 5 0.25810070089741544 1103 6 0.3594539253785811 1103 7 0.27180764094413945 1103 8 0.3277517133626233 1103 9 0.3308534519364059 1103 10-11 0.3860349274049213 1103 12-13 0.37145249702504657 1103 14-15 0.4093733221798246 1103 16-17 0.3704457382314317 1103 18-19 0.4024888124638366 1103 20-21 0.41644918038649337 1103 22-23 0.4015971836968646 1103 24-25 0.5661431023512691 1103 26-27 0.555550197003214 1103 28-29 0.4472861031233819 1103 30-31 0.40518352857764484 1103 32-33 0.3410027206235853 1103 34-35 0.34798747566397736 1103 36-37 0.44225577040053565 1103 38-39 0.5129338025032979 1103 40-41 0.47443656276758617 1103 42-43 0.4659549054494292 1103 44-45 0.2782475074911943 1103 46-47 0.09992987552902832 1103 48-49 0.1452797720717598 1103 50-51 0.12568221451480355 1103 52-53 0.253239044464884 1103 54-55 0.26047996605201007 1103 56-57 0.5574277701590802 1103 58-59 -8.512740461361322 1103 60-61 -8.056271144904038 1103 62-63 -2.6095447124885602 1103 64-65 -0.5247436647815853 1103 66-67 -1.7145879788246319 1103 68-69 -3.39768522982299 1103 70-71 -3.7399693494381694 1103 72-73 -2.842178678223867 1103 74-75 -3.6228786675245264 1103 76-77 -0.3273367956963682 1103 78-79 3.273304299948233 1103 80-81 2.7504255525971075 1103 82-83 1.3080172478964194 1103 84-85 0.887742252998386 1103 86-87 0.6721083763759736 1103 88-89 0.5492641439886938 1103 90-91 1.7783281379394218 1103 92-93 0.9879176810718384 1103 94-95 -7.468960768340809 1103 96-97 -8.619753852085463 1103 98-99 -7.930338593178216 1103 100-101 -8.994500868893729 1104 1 0.1498335934270969 1104 2 0.2086783802893848 1104 3 0.22094760943254244 1104 4 0.18592106946963582 1104 5 0.22270528304054693 1104 6 0.2600318500014822 1104 7 0.17538588601840388 1104 8 0.23682804974195193 1104 9 0.1960995744526315 1104 10-11 0.2434288422099371 1104 12-13 0.2543468371955129 1104 14-15 0.27211933829907764 1104 16-17 0.26684022290900344 1104 18-19 0.21687992595150973 1104 20-21 0.13459196230473225 1104 22-23 0.3011256338021724 1104 24-25 0.334553856349153 1104 26-27 0.370174864818523 1104 28-29 0.3303332174818081 1104 30-31 0.3362142683309486 1104 32-33 0.4397107499841013 1104 34-35 0.3372206953045449 1104 36-37 0.26337688159122763 1104 38-39 0.1447683621080671 1104 40-41 0.2775729156395528 1104 42-43 0.13947457509832617 1104 44-45 0.22157336263738614 1104 46-47 0.18908330856262268 1104 48-49 0.0462583721189489 1104 50-51 0.24939805497005807 1104 52-53 0.13832964797371972 1104 54-55 0.1429980613222952 1104 56-57 0.607470529137089 1104 58-59 -8.296705859435026 1104 60-61 -7.9216974008294585 1104 62-63 -2.4593413274485236 1104 64-65 -0.43134877394191307 1104 66-67 -1.5455110685689597 1104 68-69 -3.1146883036945177 1104 70-71 -3.5945741436338032 1104 72-73 -2.6272443025412784 1104 74-75 -3.3383990421367713 1104 76-77 0.09445364512519205 1104 78-79 3.6482090431474674 1104 80-81 3.2204865940595973 1104 82-83 1.7344808513090122 1104 84-85 1.402725764062854 1104 86-87 1.2239356757776463 1104 88-89 1.0309647916978655 1104 90-91 2.435157718759079 1104 92-93 1.680714591274132 1104 94-95 -6.431002555139665 1104 96-97 -7.649676498892154 1104 98-99 -5.966273002522287 1104 100-101 -5.4679203845154 1105 1 0.21704625628184004 1105 2 0.276006701513694 1105 3 0.22992269891073747 1105 4 0.23654189255815083 1105 5 0.21674913566887 1105 6 0.23427454634172307 1105 7 0.20683949370965138 1105 8 0.26381983097692085 1105 9 0.34338633256700746 1105 10-11 0.257339144525389 1105 12-13 0.27746672356209956 1105 14-15 0.29284768302457564 1105 16-17 0.3607526388534552 1105 18-19 0.3667049603349355 1105 20-21 0.4028829085084382 1105 22-23 0.5609381563888078 1105 24-25 0.535996592892694 1105 26-27 0.48024239009154 1105 28-29 0.607864934849708 1105 30-31 0.5257532089889736 1105 32-33 0.395411488202285 1105 34-35 0.3876223956673357 1105 36-37 0.5415015605745879 1105 38-39 0.5604147593683351 1105 40-41 0.37747395937320505 1105 42-43 0.341624786128655 1105 44-45 0.24932139441276746 1105 46-47 0.301417737879099 1105 48-49 0.079237340032428 1105 50-51 0.2313241991565107 1105 52-53 0.1525608745510425 1105 54-55 0.13791711154907205 1105 56-57 0.7710621242840219 1105 58-59 -8.44437107538361 1105 60-61 -7.6899070905620235 1105 62-63 -2.1625516047108313 1105 64-65 -0.2534637627492877 1105 66-67 -1.2183711854984587 1105 68-69 -3.163748219164141 1105 70-71 -3.272612134267323 1105 72-73 -2.3419932204149667 1105 74-75 -2.963350247607373 1105 76-77 0.5080175635568516 1105 78-79 4.096947849597434 1105 80-81 3.596026290007419 1105 82-83 2.1242009284934227 1105 84-85 1.8039818582981901 1105 86-87 1.639536105302529 1105 88-89 1.6292282902859476 1105 90-91 2.788561474367402 1105 92-93 2.102210974086333 1105 94-95 -6.351710539557933 1105 96-97 -7.495986516532826 1105 98-99 -5.622485482836344 1105 100-101 -5.160333528035979 1106 1 0.055334589782457755 1106 2 0.1598000703115794 1106 3 0.1993027383901449 1106 4 0.18225436202853018 1106 5 0.20916105978811572 1106 6 0.18119816372604447 1106 7 0.19945216057706006 1106 8 0.22781996480541267 1106 9 0.06544126916575266 1106 10-11 0.17072366725950872 1106 12-13 0.16534018871649891 1106 14-15 0.11391898015730106 1106 16-17 0.2732538934956281 1106 18-19 0.16397485895984687 1106 20-21 0.15315391121846744 1106 22-23 0.14815753475543403 1106 24-25 0.1710228567355827 1106 26-27 0.2384544413478551 1106 28-29 0.32860333359171534 1106 30-31 0.3074521617809651 1106 32-33 0.2854161721997386 1106 34-35 0.3038323725819154 1106 36-37 0.2560152612671516 1106 38-39 0.260893196251466 1106 40-41 0.36409445520779116 1106 42-43 0.42312634188495934 1106 44-45 0.4958134148885307 1106 46-47 0.34705660167514907 1106 48-49 0.28922094604133264 1106 50-51 0.27725915850610505 1106 52-53 0.509036501035709 1106 54-55 0.33361619816957244 1106 56-57 0.8358970133736534 1106 58-59 -8.326980061967308 1106 60-61 -7.856697548590063 1106 62-63 -2.2334439062264373 1106 64-65 -0.2468459434846757 1106 66-67 0.4788317988770636 1106 68-69 -2.25989395839931 1106 70-71 -2.338599621302688 1106 72-73 -0.9852532826452176 1106 74-75 -1.0341103934804394 1106 76-77 2.2224894428435427 1106 78-79 4.593970165499606 1106 80-81 3.7543052555131204 1106 82-83 2.2002054786623795 1106 84-85 1.8392741062823994 1106 86-87 1.597938862169606 1106 88-89 1.4792199260047774 1106 90-91 2.8556346450991086 1106 92-93 1.985300070409398 1106 94-95 -6.357828174808361 1106 96-97 -7.185783766357176 1106 98-99 -5.391604556743134 1106 100-101 -4.9889008939565525 1107 1 0.008280797011167351 1107 2 -0.09752907240023845 1107 3 0.003221262085048693 1107 4 -0.05451012706786429 1107 5 -0.021355757275053122 1107 6 -0.015348962198530103 1107 7 0.022294873858200503 1107 8 0.012969140353519037 1107 9 0.06200437436678641 1107 10-11 -0.05085240703738236 1107 12-13 -0.05925214280622981 1107 14-15 -0.04562002897001349 1107 16-17 -0.09962038448301058 1107 18-19 0.0354049254313864 1107 20-21 0.016183717070255454 1107 22-23 -0.09220082865157053 1107 24-25 -0.04349701664553152 1107 26-27 0.002173156569021728 1107 28-29 -0.10318226052790891 1107 30-31 -0.16518253958098228 1107 32-33 0.031091696111829492 1107 34-35 -0.07399570615765327 1107 36-37 -0.11151226078593623 1107 38-39 -0.07634245644008786 1107 40-41 -0.10212591701932894 1107 42-43 -0.11851002604800698 1107 44-45 -0.24190825537347393 1107 46-47 -0.3369672727142401 1107 48-49 -0.3801593843695983 1107 50-51 -0.29711874688083384 1107 52-53 -0.2489815510454818 1107 54-55 -0.29165986633719143 1107 56-57 0.14994381014764002 1107 58-59 -8.828469285520757 1107 60-61 -7.961294628883678 1107 62-63 -2.775584634138742 1107 64-65 -0.888850847547122 1107 66-67 0.27011397827032013 1107 68-69 -2.820169027014547 1107 70-71 -2.7968354191580893 1107 72-73 -1.4174651408437704 1107 74-75 -1.386832665778563 1107 76-77 1.5786173265684234 1107 78-79 3.725470495775493 1107 80-81 2.7581937336670883 1107 82-83 1.2693910194427325 1107 84-85 0.8407796467026536 1107 86-87 0.7075090984040742 1107 88-89 0.554803253992926 1107 90-91 1.5792696829261068 1107 92-93 0.8211692699039119 1107 94-95 -7.176718042709258 1107 96-97 -8.353682637792645 1107 98-99 -6.487581267510354 1107 100-101 -5.8897731148846795 1108 1 -0.04912978635107024 1108 2 0.02845300114447369 1108 3 0.01869654805862453 1108 4 -0.046947403744205474 1108 5 -0.07691276571524241 1108 6 0.04436757848009876 1108 7 0.018095654928977467 1108 8 0.06299318177082824 1108 9 0.029233261506092845 1108 10-11 -0.08528967270237331 1108 12-13 -0.0044245206859159225 1108 14-15 -0.09591843618024143 1108 16-17 -0.15003131378473 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2313 62-63 0.9940934410993592 2313 64-65 0.18651084929674866 2313 66-67 -1.6340669684040598 2313 68-69 -4.280258608362388 2313 70-71 -3.1824979113053793 2313 72-73 -2.934090306507983 2313 74-75 -3.618682859484462 2313 76-77 0.60131064602432 2313 78-79 3.101334120924342 2313 80-81 2.6742600007354262 2313 82-83 1.2043951280765306 2313 84-85 0.8079513516578238 2313 86-87 0.843126035051867 2313 88-89 1.1773784171115658 2313 90-91 2.7675039701950546 2313 92-93 4.037415869943214 2313 94-95 5.859599999410978 2313 96-97 0.9839840544449991 2313 98-99 2.518226338772182 2313 100-101 3.7744880371131178 2314 1 -0.35284904978335163 2314 2 -0.2637654914476961 2314 3 -0.3640981435058883 2314 4 -0.30780532665249893 2314 5 -0.3432717860201251 2314 6 -0.4340367889081378 2314 7 -0.4341262924896512 2314 8 -0.5101569142393458 2314 9 -0.46175836671480397 2314 10-11 -0.47864099230494617 2314 12-13 -0.4690447480051603 2314 14-15 -0.6819992707790661 2314 16-17 -0.524279921107933 2314 18-19 -0.565963029092309 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0.7463836013577811 2314 86-87 0.6864261024502767 2314 88-89 0.7019400194106957 2314 90-91 2.3050499336311177 2314 92-93 3.790857887942913 2314 94-95 6.258854719091499 2314 96-97 2.8437539286204085 2314 98-99 5.370940117321579 2314 100-101 5.825059876658033 2315 1 -0.416730177524407 2315 2 -0.4254005250149149 2315 3 -0.5486691911990853 2315 4 -0.3563763870320926 2315 5 -0.48534805934265535 2315 6 -0.5895497151837787 2315 7 -0.5139108272976358 2315 8 -0.7095520232305645 2315 9 -0.7888014701026407 2315 10-11 -0.6126854791783529 2315 12-13 -0.6871569328607308 2315 14-15 -0.6904019675743811 2315 16-17 -0.7325144896851228 2315 18-19 -0.7608730357593458 2315 20-21 -0.7481460499767181 2315 22-23 -0.6193404810890684 2315 24-25 -0.45778903733846477 2315 26-27 -0.5208379137848382 2315 28-29 -0.7325908166228245 2315 30-31 -0.7419428105206975 2315 32-33 -0.6727715632440621 2315 34-35 -0.5696781933455455 2315 36-37 -0.6090758581798141 2315 38-39 -0.6600518606063659 2315 40-41 -0.7267502441028313 2315 42-43 -0.5608128974449187 2315 44-45 -0.6960336222922265 2315 46-47 -0.5994694095255539 2315 48-49 -0.4663187203011674 2315 50-51 -0.5794540739347198 2315 52-53 -0.4726633573187442 2315 54-55 -0.3599319030295476 2315 56-57 0.4000207671882592 2315 58-59 3.5506691648907314 2315 60-61 3.65461425366189 2315 62-63 0.9309115985475138 2315 64-65 0.2219208938912267 2315 66-67 -1.4590260876819414 2315 68-69 -4.251732372288558 2315 70-71 -3.1859493165332076 2315 72-73 -2.9313475304307097 2315 74-75 -0.8063451114908204 2315 76-77 1.8468547126966754 2315 78-79 3.626495584248236 2315 80-81 2.626794719926874 2315 82-83 1.0955681040394474 2315 84-85 0.8272881702320909 2315 86-87 0.8247904345629351 2315 88-89 0.9922845320241507 2315 90-91 2.7112645765439396 2315 92-93 4.18966329622306 2315 94-95 6.490218769432477 2315 96-97 3.5627317100557256 2315 98-99 5.898394660773006 2315 100-101 6.3599385946503215 2316 1 -0.42229139719646014 2316 2 -0.37746389335388386 2316 3 -0.5158207902511833 2316 4 -0.355166771011568 2316 5 -0.5701465327490496 2316 6 -0.5142287740701121 2316 7 -0.4660492848638569 2316 8 -0.5187709419526598 2316 9 -0.7586410370033079 2316 10-11 -0.7551885864089058 2316 12-13 -0.6250024347721137 2316 14-15 -0.6284240424134211 2316 16-17 -0.7989107451195068 2316 18-19 -0.6653478223299771 2316 20-21 -0.5866046441042911 2316 22-23 -0.8633379597635482 2316 24-25 -0.7218797224762667 2316 26-27 -0.800642631269902 2316 28-29 -0.5988601259657074 2316 30-31 -0.5471514679775851 2316 32-33 -0.39133626838034274 2316 34-35 -0.37450609092731924 2316 36-37 -0.4053476780465033 2316 38-39 -0.5272860490974054 2316 40-41 -0.670320341377348 2316 42-43 -0.6484511286981345 2316 44-45 -0.6386314360284402 2316 46-47 -0.678322680297299 2316 48-49 -0.744480142278583 2316 50-51 -0.602667011303339 2316 52-53 -0.5746736405357993 2316 54-55 -0.4208258690264941 2316 56-57 0.25945739240312093 2316 58-59 3.3786373061518624 2316 60-61 3.3356476295167568 2316 62-63 0.920677294025328 2316 64-65 0.44243939981421576 2316 66-67 -1.5507639991003472 2316 68-69 -2.9188912276430123 2316 70-71 -3.187308414124466 2316 72-73 -1.1045283327460353 2316 74-75 5.320754076674419 2316 76-77 5.89640593696468 2316 78-79 5.03239680480533 2316 80-81 3.4764211379546985 2316 82-83 1.859301984682073 2316 84-85 1.2309793981029813 2316 86-87 1.1337988943835775 2316 88-89 1.0976449969013835 2316 90-91 2.3571478314371994 2316 92-93 4.042837898014959 2316 94-95 6.285262592968962 2316 96-97 3.0723693949520587 2316 98-99 5.611687788558148 2316 100-101 5.804745529968011 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 28.0 4 70.0 5 170.0 6 389.0 7 1040.0 8 2143.0 9 3757.0 10 5849.0 11 8944.0 12 12753.0 13 17001.0 14 21718.0 15 28688.0 16 38500.0 17 50050.0 18 55268.0 19 56450.0 20 60065.0 21 61303.0 22 61746.0 23 62806.0 24 65298.0 25 67058.0 26 82181.0 27 116051.0 28 163182.0 29 212561.0 30 248835.0 31 266543.0 32 268367.0 33 260220.0 34 261456.0 35 268547.0 36 245816.0 37 157665.0 38 46503.0 39 1741.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.14113638019708 20.470215775222787 14.79941255040868 23.589235294171452 2 15.891111669981786 21.660943322892614 38.99241714107166 23.45552786605394 3 18.73117049850705 25.68642715276601 30.880534852332815 24.701867496394122 4 13.292452611647775 17.98971215241328 37.2831450235372 31.434690212401744 5 13.267175511989135 38.058303829270976 34.96570676931813 13.708813889421753 6 31.374003128539574 37.59892512841521 17.421338444337966 13.605733298707252 7 27.198329413514656 33.095675275637014 21.96915108797829 17.736844222870037 8 25.553004318205442 35.98427316101356 20.115759727697323 18.346962793083677 9 26.01814699259075 16.211559258758022 20.35172295233671 37.41857079631451 10-11 24.481020274545806 26.67954781264364 28.365176525955537 20.474255386855013 12-13 25.392439078214707 24.109231874039097 28.59428474587017 21.904044301876027 14-15 22.42391378561988 25.42458253473594 26.479706481928535 25.671797197715645 16-17 22.0338649829529 28.53341912637104 27.053138697745204 22.379577192930853 18-19 21.9360616297539 27.308730648116452 29.168821913749674 21.586385808379966 20-21 22.691315193656113 26.64577519138835 29.027522247866656 21.635387367088885 22-23 22.382408487779067 26.487290765199813 28.99259135981741 22.137709387203714 24-25 22.329416153930094 26.729643905897472 28.99640388421958 21.944536055952852 26-27 22.26399267365214 26.866506449798315 28.896553437365075 21.97294743918447 28-29 22.191471919405657 26.970626780379288 28.947018290003758 21.890883010211308 30-31 22.37210860465598 26.701504848509234 28.86794045959941 22.058446087235367 32-33 22.17523180772148 26.792304213789382 28.840939292797646 22.191524685691487 34-35 22.542126654600146 26.836969648569113 28.7179695077456 21.90293418908514 36-37 22.474754176775523 26.67713930191177 28.71285120163804 22.135255319674673 38-39 22.276651693732184 26.870964785006386 28.882603011943726 21.969780509317705 40-41 22.460099164286092 26.706101149280126 28.84452268092861 21.98927700550518 42-43 22.313417901314036 26.840851447562137 28.561629459968508 22.284101191155322 44-45 22.43368476415665 26.868983068505596 28.444785517981025 22.252546649356727 46-47 22.455652949562946 26.94835765251546 28.201379087755736 22.39461031016586 48-49 22.501657097241488 27.122159713975712 28.091740807723948 22.284442381058852 50-51 22.35941674720461 27.236592129482133 28.031534778893423 22.372456344419838 52-53 22.610675689441365 27.219262818811924 27.816856948884567 22.353204542862144 54-55 22.453781389597953 26.99308346635728 27.96338953299637 22.5897456110484 56-57 22.399981576918197 27.318258128117286 28.031069734414803 22.250690560549717 58-59 22.20192687718208 26.550866505115994 29.41922522622673 21.8279813914752 60-61 22.16838766144697 26.902437267511242 28.84107789905635 22.088097171985442 62-63 22.51566838411035 27.115868161532575 28.03253073856378 22.335932715793298 64-65 22.40161417705653 27.08669328105963 27.992054088769986 22.51963845311385 66-67 23.00840261017252 26.177737090156988 29.168585457874443 21.64527484179605 68-69 23.01783253180544 25.04764121903438 31.05837436996879 20.876151879191394 70-71 23.939675473110363 24.93837341395914 30.298868068462436 20.82308304446806 72-73 23.03446393878376 25.49961100317717 30.029763068958303 21.43616198908077 74-75 22.83660103674302 26.057764131853556 29.302920475504806 21.802714355898615 76-77 21.301512403142908 26.895816424702428 29.23118048268786 22.571490689466795 78-79 22.319668137602065 26.786483047256244 28.534256528824393 22.359592286317298 80-81 22.01869998412878 27.2946068504201 27.784769820841245 22.901923344609877 82-83 22.303942822978286 27.321090534399282 27.564616536084568 22.810350106537864 84-85 22.27201990295117 27.23620535068603 27.451356325952283 23.04041842041052 86-87 22.18413774057996 27.18255576980405 27.654586587448236 22.978719902167757 88-89 22.179069984351194 27.202628535687744 27.43768063638874 23.180620843572317 90-91 22.23847655186798 26.987141494933482 28.058703971805848 22.715677981392695 92-93 21.981910355068294 27.213119353657195 27.669142114234113 23.1358281770404 94-95 21.44230406697368 26.5920907345637 29.048171958618514 22.917433239844108 96-97 21.591119027131313 26.178362152551326 29.556958768638676 22.67356005167869 98-99 21.642612604196817 26.60766008725206 28.36985044191277 23.379876866638348 100-101 20.98244827676596 26.82938456989242 28.712555294159486 23.475611859182138 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 829.0 1 687.0 2 575.0 3 637.5 4 687.0 5 784.0 6 980.0 7 1457.5 8 2184.5 9 2709.5 10 2956.0 11 3145.5 12 3327.0 13 3440.0 14 3653.0 15 3969.5 16 4257.5 17 4571.0 18 4966.0 19 5467.5 20 6097.0 21 7020.5 22 8353.0 23 10006.5 24 12152.5 25 15046.0 26 18729.5 27 23299.5 28 28049.0 29 32993.5 30 38654.5 31 44810.5 32 51113.0 33 57376.0 34 64434.0 35 71302.5 36 78286.0 37 85125.5 38 90619.0 39 95431.5 40 99391.5 41 104127.0 42 109031.5 43 113421.5 44 118761.5 45 123481.5 46 126454.0 47 129335.5 48 131849.0 49 131929.0 50 129947.0 51 127106.5 52 122897.0 53 117847.5 54 111260.0 55 102217.0 56 91977.5 57 81348.0 58 70731.5 59 59797.5 60 48670.5 61 38104.0 62 28864.5 63 21684.5 64 16231.5 65 11676.5 66 8190.0 67 5663.0 68 3690.5 69 2230.5 70 1304.5 71 763.5 72 439.5 73 259.0 74 141.5 75 73.5 76 38.5 77 24.0 78 12.5 79 4.5 80 4.0 81 1.5 82 3.0 83 3.5 84 1.5 85 1.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025085620300637292 2 0.010332959030274655 3 2.4384564083244025E-4 4 0.0 5 0.05599305527614909 6 0.0 7 0.0 8 9.14421153121651E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 3.657684612486604E-4 16-17 1.5240352552027516E-5 18-19 3.3528775614460535E-4 20-21 0.0 22-23 0.0 24-25 6.096141020811006E-5 26-27 7.620176276013757E-5 28-29 0.0011430264414020637 30-31 0.005120758457481245 32-33 0.005776093617218429 34-35 0.0018898037164514119 36-37 0.08220646166563642 38-39 0.07667421368925044 40-41 0.08133776157017085 42-43 0.08443155313823243 44-45 0.0877387096420224 46-47 0.0831361231713101 48-49 0.07440340115899832 50-51 0.06975509363062994 52-53 0.05943737495290731 54-55 0.048540522878207634 56-57 0.06913023917599681 58-59 0.7439425694746711 60-61 0.3470380679622186 62-63 0.0034443196767582184 64-65 0.0025298985236365677 66-67 1.1135820802715464 68-69 2.390982710124837 70-71 2.346785687723957 72-73 1.8184179050977152 74-75 1.2909493032720427 76-77 0.7404068076826008 78-79 0.5586351227945685 80-81 0.03787227609178838 82-83 1.6764387807230267E-4 84-85 0.001356391377130449 86-87 0.0014021124347865315 88-89 6.096141020811006E-5 90-91 0.33120334166066195 92-93 0.05367652168824091 94-95 0.967046090483802 96-97 1.666563032269313 98-99 0.6439810970859227 100-101 0.5792705601500139 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3280764.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.80502781270525 #Duplication Level Percentage of deduplicated Percentage of total 1 69.773050348441 34.05275662832947 2 14.925189033941658 14.56848531822812 3 5.931716927848709 8.68492828922252 4 2.989707614227858 5.836510530569891 5 1.7364522212386349 4.237379947649268 6 1.0967767776260196 3.2116932681820276 7 0.7357409531554748 2.5135500377189453 8 0.528304871954215 2.062714717545052 9 0.3771312060432731 1.6565309099982914 >10 1.7731978000709345 14.705072701225152 >50 0.0853663973062867 2.8332545602096246 >100 0.044988367552223164 3.8601386687746513 >500 0.0016266972499076246 0.49842283317259606 >1k 5.630875079572366E-4 0.37750422542422296 >5k 1.2513055732383036E-4 0.5175377430004979 >10k+ 6.256527866191518E-5 0.3835196207496757 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12560 0.38283765610693116 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9419 0.28709776137509435 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7530 0.2295197094335344 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.048070510405503E-5 0.0 4 0.0 0.0 0.0 3.048070510405503E-5 0.0 5 0.0 0.0 0.0 3.048070510405503E-5 0.0 6 0.0 0.0 0.0 6.096141020811006E-5 0.0 7 0.0 0.0 0.0 6.096141020811006E-5 0.0 8 0.0 0.0 0.0 6.096141020811006E-5 0.0 9 0.0 0.0 0.0 1.5240352552027515E-4 3.048070510405503E-5 10-11 0.0 0.0 0.0 1.828842306243302E-4 3.048070510405503E-5 12-13 0.0 0.0 0.0 2.4384564083244025E-4 3.048070510405503E-5 14-15 0.0 0.0 0.0 2.4384564083244025E-4 3.048070510405503E-5 16-17 0.0 0.0 0.0 5.181719867689355E-4 3.048070510405503E-5 18-19 0.0 0.0 0.0 8.077386852574583E-4 3.048070510405503E-5 20-21 0.0 0.0 0.0 9.449018582257059E-4 3.048070510405503E-5 22-23 0.0 0.0 0.0 0.0011735071465061187 3.048070510405503E-5 24-25 0.0 0.0 0.0 0.0015697563128588342 4.572105765608255E-5 26-27 1.5240352552027516E-5 0.0 0.0 0.0017678808960351917 6.096141020811006E-5 28-29 3.048070510405503E-5 0.0 0.0 0.003017589805301448 6.096141020811006E-5 30-31 3.048070510405503E-5 0.0 0.0 0.00736109028262929 6.096141020811006E-5 32-33 3.048070510405503E-5 0.0 0.0 0.016078571942389028 6.096141020811006E-5 34-35 3.048070510405503E-5 0.0 0.0 0.029230996194788774 6.096141020811006E-5 36-37 3.048070510405503E-5 0.0 0.0 0.0489062913394563 6.096141020811006E-5 38-39 3.048070510405503E-5 0.0 0.0 0.0802556965389769 6.096141020811006E-5 40-41 3.048070510405503E-5 0.0 0.0 0.11594860221582534 7.620176276013757E-5 42-43 3.048070510405503E-5 0.0 0.0 0.15331794667339682 9.14421153121651E-5 44-45 3.048070510405503E-5 0.0 0.0 0.19911520609223948 9.14421153121651E-5 46-47 3.048070510405503E-5 0.0 0.0 0.2520449505054311 9.14421153121651E-5 48-49 3.048070510405503E-5 0.0 0.0 0.30570623184111995 9.14421153121651E-5 50-51 3.048070510405503E-5 0.0 0.0 0.3595503974074331 9.14421153121651E-5 52-53 3.048070510405503E-5 0.0 0.0 0.4158025386769667 9.14421153121651E-5 54-55 3.048070510405503E-5 0.0 0.0 0.48007110538886677 9.14421153121651E-5 56-57 3.048070510405503E-5 0.0 0.0 0.5391731925856295 1.0668246786419261E-4 58-59 3.048070510405503E-5 0.0 0.0 0.5837969448579661 1.2192282041622012E-4 60-61 4.572105765608255E-5 3.048070510405503E-5 1.5240352552027516E-5 0.621989268353347 1.2192282041622012E-4 62-63 9.14421153121651E-5 3.048070510405503E-5 3.048070510405503E-5 0.6555637650254635 1.6764387807230267E-4 64-65 9.14421153121651E-5 3.048070510405503E-5 3.048070510405503E-5 0.6942895008601655 1.828842306243302E-4 66-67 1.0668246786419261E-4 3.048070510405503E-5 3.048070510405503E-5 0.729464234550245 1.828842306243302E-4 68-69 1.3716317296824762E-4 4.572105765608255E-5 3.048070510405503E-5 0.7709179934917598 2.1336493572838522E-4 70-71 1.5240352552027515E-4 6.096141020811006E-5 4.572105765608255E-5 0.8363143462925099 2.1336493572838522E-4 72-73 1.5240352552027515E-4 6.096141020811006E-5 6.096141020811006E-5 0.9294938617956061 2.1336493572838522E-4 74-75 1.5240352552027515E-4 6.096141020811006E-5 6.096141020811006E-5 1.0598141164679933 2.1336493572838522E-4 76-77 1.5240352552027515E-4 6.096141020811006E-5 6.096141020811006E-5 1.235611583155631 2.1336493572838522E-4 78-79 1.5240352552027515E-4 6.096141020811006E-5 6.096141020811006E-5 1.4553927073084196 2.1336493572838522E-4 80-81 1.5240352552027515E-4 6.096141020811006E-5 6.096141020811006E-5 1.7163380237042347 2.2860528828041272E-4 82-83 1.5240352552027515E-4 6.096141020811006E-5 6.096141020811006E-5 1.9791731438165012 2.4384564083244025E-4 84-85 1.5240352552027515E-4 7.620176276013757E-5 6.096141020811006E-5 2.2148956767387107 2.4384564083244025E-4 86-87 1.5240352552027515E-4 9.14421153121651E-5 6.096141020811006E-5 2.434890165827228 2.4384564083244025E-4 88-89 1.5240352552027515E-4 1.0668246786419261E-4 6.096141020811006E-5 2.645847125852393 2.743263459364953E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6910 0.0 40.757538 1 GTATCAA 11300 0.0 36.445797 1 TCAACGC 14380 0.0 28.140648 4 TATCAAC 14570 0.0 28.099525 2 ATCAACG 14445 0.0 28.07932 3 CAACGCA 14740 0.0 27.45336 5 AACGCAG 15115 0.0 26.754293 6 ACGCAGA 17075 0.0 23.269188 7 CGCAGAG 17085 0.0 22.952116 8 GTACATG 9830 0.0 22.89162 1 TACATGG 9685 0.0 22.646032 2 ACATGGG 9840 0.0 21.233145 3 GCAGAGT 18255 0.0 20.422815 9 AGTACTT 10765 0.0 20.14086 12-13 GAGTACT 10095 0.0 20.006851 12-13 AGAGTAC 15140 0.0 19.908672 10-11 CATGGGG 5785 0.0 18.099031 4 GTACTTT 11115 0.0 18.043648 14-15 GAGTACA 8500 0.0 17.53799 1 CAGAGTA 17405 0.0 17.060593 10-11 >>END_MODULE