FastQCFastQC Report
Fri 27 May 2016
SRR939569_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939569_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2943844
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90400.30708148937239876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60770.20643077554381278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43670.14834345841695415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62050.036.6317981
GTATCAA103100.035.2561261
TCAACGC119700.029.8915944
ATCAACG119800.029.8656273
TATCAAC123300.029.1722972
CAACGCA121950.029.146025
AACGCAG126400.028.1921256
ACGCAGA137800.025.308897
CGCAGAG138500.024.8387978
TACATGG80450.023.7117982
GTACATG84050.023.5995141
ACATGGG81800.021.8118063
GCAGAGT146850.021.7785159
GAGTACT77900.021.04555112-13
AGAGTAC115150.020.93386810-11
GTACTTT85900.018.97651914-15
AGTACTT85100.018.95828612-13
CAGAGTA138450.018.26770410-11
TAGCCCT21350.018.0035844
CATGGGG46350.017.404884
Produced by FastQC (version 0.11.5)