FastQCFastQC Report
Fri 27 May 2016
SRR939569_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939569_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2943844
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91650.3113276382851809No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69600.23642557146370527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52260.1775229937455925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58600.040.085761
GTATCAA93900.033.9398771
ATCAACG114250.027.43343
TCAACGC114900.027.2372474
TATCAAC117100.026.9278492
CAACGCA118300.026.4544355
AACGCAG122950.025.4553956
ACGCAGA138050.022.36477
CGCAGAG136550.022.1629898
TACATGG81450.022.0149482
GTACATG85750.021.5747411
ACATGGG80450.020.7080333
GCAGAGT147850.019.4516689
AGAGTAC117750.019.156110-11
GAGTACT80850.019.06428712-13
AGTACTT88900.017.76087212-13
GGACCGA3752.0008883E-1017.5440926
GTACTTT89550.017.31709714-15
CAGAGTA138700.017.12661410-11
CATGGGG45400.016.689054
Produced by FastQC (version 0.11.5)