##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939569_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2943844 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.46167324083749 31.0 31.0 34.0 30.0 34.0 2 31.685824045024123 31.0 31.0 34.0 30.0 34.0 3 31.729582138183954 31.0 31.0 34.0 30.0 34.0 4 35.5316878204144 37.0 35.0 37.0 33.0 37.0 5 35.25621432385684 37.0 35.0 37.0 33.0 37.0 6 35.27545039750748 37.0 35.0 37.0 32.0 37.0 7 35.20337830401339 37.0 35.0 37.0 32.0 37.0 8 35.16530155809887 37.0 35.0 37.0 32.0 37.0 9 36.76975206566652 39.0 35.0 39.0 32.0 39.0 10-11 36.64473559060874 39.0 35.0 39.0 32.0 39.0 12-13 36.58755423181391 39.0 35.0 39.0 32.0 39.0 14-15 37.64193466093991 39.0 36.0 41.0 32.0 41.0 16-17 37.59285648288429 39.0 36.0 41.0 32.0 41.0 18-19 37.564390130727034 39.0 36.0 41.0 32.0 41.0 20-21 37.49550604583667 39.0 36.0 41.0 32.0 41.0 22-23 37.35617631233177 39.0 36.0 41.0 31.5 41.0 24-25 37.23535927854873 39.0 36.0 40.5 31.0 41.0 26-27 36.95967907946209 39.0 36.0 40.0 31.0 41.0 28-29 36.75522378223846 39.0 36.0 40.0 30.0 41.0 30-31 36.52169985909579 38.0 35.0 40.0 30.0 41.0 32-33 36.3512482319036 38.0 35.0 40.0 30.0 41.0 34-35 36.34376396982992 38.0 35.0 40.0 30.0 41.0 36-37 36.25319836920706 38.0 35.0 40.0 30.0 41.0 38-39 36.29858256755453 38.0 35.0 40.0 30.0 41.0 40-41 36.20937658381355 38.0 35.0 40.0 30.0 41.0 42-43 36.024780185363085 38.0 35.0 40.0 29.0 41.0 44-45 35.81946342944803 38.0 35.0 40.0 28.5 41.0 46-47 35.54653864131387 38.0 34.0 40.0 27.5 41.0 48-49 35.25793690154777 38.0 34.0 40.0 27.0 41.0 50-51 35.01106308622332 38.0 34.0 40.0 26.0 41.0 52-53 34.77097767408871 38.0 33.5 40.0 26.0 41.0 54-55 34.47411496668981 38.0 33.0 40.0 25.5 41.0 56-57 32.29034266082034 36.5 30.0 39.5 16.0 40.5 58-59 27.078656171998247 32.5 17.0 38.0 6.5 40.0 60-61 27.85231367558879 32.0 19.0 38.0 8.0 40.0 62-63 31.034171817528375 34.5 29.0 38.0 8.5 40.0 64-65 31.280513845162993 35.0 30.5 39.0 5.0 40.0 66-67 24.454368505939854 26.5 17.0 35.5 2.0 38.5 68-69 16.96095700043888 13.0 7.0 28.0 2.0 34.5 70-71 15.811615527181468 12.5 7.0 25.5 2.0 33.5 72-73 16.842809775246245 13.0 7.0 28.0 2.0 34.0 74-75 17.840834127080104 17.0 7.5 29.0 2.0 34.5 76-77 19.110261617123733 21.0 8.0 30.0 2.0 34.0 78-79 22.150398594490742 27.0 10.0 33.0 2.0 35.5 80-81 25.80461447685407 31.0 22.0 34.5 2.0 36.0 82-83 27.162234989354054 33.0 26.0 35.0 2.0 36.0 84-85 27.229233784127146 33.0 26.0 35.0 2.0 36.0 86-87 26.980918825861696 33.0 26.0 35.0 2.0 36.0 88-89 26.52672526125705 33.0 25.5 35.0 2.0 35.0 90-91 25.070735066124428 32.5 13.0 34.5 2.0 35.0 92-93 22.393898419889098 31.0 2.0 34.0 2.0 35.0 94-95 15.08126636465791 4.5 2.0 31.5 2.0 34.0 96-97 12.57462776560171 2.0 2.0 28.5 2.0 34.0 98-99 12.173433442804715 2.0 2.0 29.0 2.0 34.0 100-101 11.880106078990599 2.0 2.0 29.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37448117090045585 1101 2 0.34578827373258747 1101 3 0.39864378740281836 1101 4 0.2945800795409852 1101 5 0.37445487368896124 1101 6 0.41078020580403063 1101 7 0.36880938535891516 1101 8 0.3384681658870008 1101 9 0.4064652990731261 1101 10-11 0.449616123528493 1101 12-13 0.45526827683640647 1101 14-15 0.6061100738624603 1101 16-17 0.6066874889621658 1101 18-19 0.4148651858249295 1101 20-21 0.45827977131882847 1101 22-23 0.4973265191566014 1101 24-25 0.5587387743002807 1101 26-27 0.4741151985387262 1101 28-29 0.6238945091112598 1101 30-31 0.6096794461549635 1101 32-33 0.5661069593712895 1101 34-35 0.4764165193210701 1101 36-37 0.3509624641488003 1101 38-39 0.43286520282350693 1101 40-41 0.5183314598185262 1101 42-43 0.5668260251226371 1101 44-45 0.39095255498939707 1101 46-47 0.4144670744144108 1101 48-49 0.3113190440690943 1101 50-51 0.34512874527919735 1101 52-53 0.31395576623543064 1101 54-55 0.045485415650865946 1101 56-57 -2.0710366653991343 1101 58-59 -9.503871269755745 1101 60-61 -10.004401609718567 1101 62-63 -3.805663199986011 1101 64-65 -5.218196818856352 1101 66-67 -9.863950239888954 1101 68-69 -4.976767484994074 1101 70-71 -4.650704420803946 1101 72-73 -4.912106211050059 1101 74-75 -6.700873812204282 1101 76-77 -8.140040038668499 1101 78-79 -11.061381574017378 1101 80-81 -8.20682036892764 1101 82-83 -4.603310375764227 1101 84-85 -3.8809356452492274 1101 86-87 -3.5569589495285996 1101 88-89 -3.6665507790081264 1101 90-91 -9.947771978787657 1101 92-93 -16.505254323797775 1101 94-95 -10.84174868148633 1101 96-97 -9.110464985750939 1101 98-99 -9.075411167421242 1101 100-101 -8.837413112589203 1102 1 0.34875060379813405 1102 2 0.36153751851158233 1102 3 0.32603139366428735 1102 4 0.255664742312959 1102 5 0.3018775883652154 1102 6 0.2543316482137499 1102 7 0.32192872900319713 1102 8 0.380921651138344 1102 9 0.4554072094702093 1102 10-11 0.39636621559056096 1102 12-13 0.2991159952575586 1102 14-15 0.36638045546752096 1102 16-17 0.40823366354928936 1102 18-19 0.44597962833450566 1102 20-21 0.5997405466374559 1102 22-23 0.6005614475042265 1102 24-25 0.5118382231761913 1102 26-27 0.38106795197466425 1102 28-29 0.4387165406557827 1102 30-31 0.4006536968633725 1102 32-33 0.3452845876652404 1102 34-35 0.22481186971865696 1102 36-37 0.13321012826893508 1102 38-39 0.15165869824848244 1102 40-41 0.06318245970618364 1102 42-43 0.2588191220281004 1102 44-45 0.24778278005774723 1102 46-47 0.38368713707902913 1102 48-49 0.27639182771387283 1102 50-51 0.25792275874834303 1102 52-53 0.25622309886971806 1102 54-55 0.02006965400111227 1102 56-57 -2.98156735639515 1102 58-59 -10.30264329443462 1102 60-61 -10.549316389893079 1102 62-63 -4.578401749961671 1102 64-65 -5.816873114590912 1102 66-67 -7.06859718966988 1102 68-69 -4.5415821047621385 1102 70-71 -4.338026928262531 1102 72-73 -4.769492709445995 1102 74-75 -5.585485325891884 1102 76-77 -7.761224144074882 1102 78-79 -10.420419533235444 1102 80-81 -6.290904437197124 1102 82-83 -3.7235352412599845 1102 84-85 -3.2922814366085014 1102 86-87 -3.14284134882206 1102 88-89 -3.3319363786286402 1102 90-91 -9.199625703088298 1102 92-93 -13.379131634711655 1102 94-95 -10.193406071729623 1102 96-97 -8.894297794798613 1102 98-99 -9.095089433908154 1102 100-101 -8.971152767309645 1103 1 0.24141735591175717 1103 2 0.2498943778093512 1103 3 0.2472723855912733 1103 4 0.17581466720162808 1103 5 0.20666043872903828 1103 6 0.28140053270369947 1103 7 0.24112309364679874 1103 8 0.31845830594169655 1103 9 0.44485289174475895 1103 10-11 0.4265640555086989 1103 12-13 0.3892745662564323 1103 14-15 0.4954909720461771 1103 16-17 0.47104408307228596 1103 18-19 0.4789180284135668 1103 20-21 0.36280082299364125 1103 22-23 0.40881696153025615 1103 24-25 0.5002109095811065 1103 26-27 0.6121853068993275 1103 28-29 0.5304791841084935 1103 30-31 0.5896577305639923 1103 32-33 0.5549787069724985 1103 34-35 0.5847862593662754 1103 36-37 0.4790560466878091 1103 38-39 0.2782747462230688 1103 40-41 0.5435617557354391 1103 42-43 0.4728028512872555 1103 44-45 0.6216900947058193 1103 46-47 0.49990676079305985 1103 48-49 0.46346871961959124 1103 50-51 0.40776829610830134 1103 52-53 0.4640694816367059 1103 54-55 0.46554230299062027 1103 56-57 -2.7761247627260843 1103 58-59 -10.105580112881842 1103 60-61 -10.273251345337666 1103 62-63 -4.090924854085696 1103 64-65 -5.290685941301458 1103 66-67 -6.600479262509527 1103 68-69 -4.229946526978868 1103 70-71 -4.0402508040416425 1103 72-73 -4.214399488108597 1103 74-75 -4.935030223737456 1103 76-77 -6.629726614916105 1103 78-79 -9.202926868511618 1103 80-81 -4.956727746695936 1103 82-83 -2.6823613510886766 1103 84-85 -2.175682803846154 1103 86-87 -2.0157618158285295 1103 88-89 -2.0491541997570835 1103 90-91 -5.310821487963928 1103 92-93 -10.13760976395838 1103 94-95 -8.382353032903474 1103 96-97 -8.013826654077397 1103 98-99 -8.153378339138413 1103 100-101 -8.162097752414688 1104 1 0.2671793541148766 1104 2 0.3063366348250902 1104 3 0.27860950963091824 1104 4 0.23640667486051825 1104 5 0.18884837898416862 1104 6 0.2611733300718555 1104 7 0.316393387694049 1104 8 0.3804123997379065 1104 9 0.33201338100674604 1104 10-11 0.36760212199654774 1104 12-13 0.3643173955759025 1104 14-15 0.427571808526352 1104 16-17 0.4347381078133239 1104 18-19 0.4182278871621463 1104 20-21 0.4088856685256772 1104 22-23 0.39296523712189213 1104 24-25 0.39822841799561104 1104 26-27 0.5018454091126685 1104 28-29 0.4345527339154529 1104 30-31 0.5339508659668226 1104 32-33 0.5673403396229091 1104 34-35 0.5291941379293164 1104 36-37 0.4530606002490387 1104 38-39 0.4993593174686666 1104 40-41 0.434104450756152 1104 42-43 0.5649948408229193 1104 44-45 0.5091726673582002 1104 46-47 0.545324023609453 1104 48-49 0.5913121557013028 1104 50-51 0.5676386358293257 1104 52-53 0.5801500422050481 1104 54-55 0.5262446347504266 1104 56-57 -2.616403350382541 1104 58-59 -10.242060828919797 1104 60-61 -10.451381441224147 1104 62-63 -4.201520041435526 1104 64-65 -5.30752453815769 1104 66-67 -6.808207354032614 1104 68-69 -4.127324541640796 1104 70-71 -4.1393770595116255 1104 72-73 -4.236074008848917 1104 74-75 -4.957272060261925 1104 76-77 -6.71610373195027 1104 78-79 -9.436307192868536 1104 80-81 -5.25345477805114 1104 82-83 -2.709079389912194 1104 84-85 -2.2280075134784028 1104 86-87 -1.9852797035710168 1104 88-89 -2.116951102636527 1104 90-91 -4.705600181474633 1104 92-93 -6.873139935263467 1104 94-95 -7.747852918922222 1104 96-97 -7.7040985471129995 1104 98-99 -7.856246836873621 1104 100-101 -7.807284523193713 1105 1 0.3800985545738449 1105 2 0.3613688347728967 1105 3 0.37173172792815734 1105 4 0.27548753182364294 1105 5 0.35949533620317453 1105 6 0.33885690258697565 1105 7 0.3466801546203655 1105 8 0.3044775348413111 1105 9 0.5210948788413745 1105 10-11 0.5578795885956112 1105 12-13 0.510139297897851 1105 14-15 0.5857247450764831 1105 16-17 0.5397044518054628 1105 18-19 0.5442779807550195 1105 20-21 0.5681237799747691 1105 22-23 0.6265757687427538 1105 24-25 0.6106406121909487 1105 26-27 0.6549334612683353 1105 28-29 0.6609484366925855 1105 30-31 0.6591956660636171 1105 32-33 0.6203518460862227 1105 34-35 0.64633657905501 1105 36-37 0.6486358695816961 1105 38-39 0.6235091501499355 1105 40-41 0.5947326894002885 1105 42-43 0.45146722259366356 1105 44-45 0.4672142973456488 1105 46-47 0.5443190858226927 1105 48-49 0.4050147574857945 1105 50-51 0.331865803756628 1105 52-53 0.51678502589359 1105 54-55 0.42824297463688055 1105 56-57 1.6384664783612095 1105 58-59 -8.41554874387769 1105 60-61 -9.50539578341569 1105 62-63 -3.519943251442964 1105 64-65 -4.828205307757205 1105 66-67 -6.104021591531389 1105 68-69 -3.940602835220444 1105 70-71 -3.9832944455351917 1105 72-73 -2.20377188048902 1105 74-75 -4.223189898484424 1105 76-77 -6.310226171220005 1105 78-79 -9.167444780802516 1105 80-81 -4.45838969370476 1105 82-83 -1.9208407662785945 1105 84-85 -1.3058104931720038 1105 86-87 -1.2320296903690604 1105 88-89 -1.25195186399187 1105 90-91 -4.223727821544795 1105 92-93 -6.8039531909471 1105 94-95 -7.666778162030418 1105 96-97 -7.859577203784806 1105 98-99 -8.091008681332568 1105 100-101 -7.8807955095257185 1106 1 0.13077271885866892 1106 2 0.08431141778734386 1106 3 0.07486750780757134 1106 4 0.036324725380460166 1106 5 0.07652224278219677 1106 6 0.16666036757499825 1106 7 0.22132641388169105 1106 8 0.10364749751748548 1106 9 0.16409334046553425 1106 10-11 0.23198720205618173 1106 12-13 0.17510353815873714 1106 14-15 0.15698073895726594 1106 16-17 0.23334531389585322 1106 18-19 0.09411648343111523 1106 20-21 0.2965364353657307 1106 22-23 0.3179002289185249 1106 24-25 0.31582341751998655 1106 26-27 0.3062251184719216 1106 28-29 0.34034555620167595 1106 30-31 0.38098044985539303 1106 32-33 0.26508944373452437 1106 34-35 0.31246633184358785 1106 36-37 0.33935001449756186 1106 38-39 0.40541336161447816 1106 40-41 0.22661874245925162 1106 42-43 0.24402704355928506 1106 44-45 0.11014157159220872 1106 46-47 0.37045079907630196 1106 48-49 0.2536415749332903 1106 50-51 0.33018017878608674 1106 52-53 0.33191451300165653 1106 54-55 0.22429820832859804 1106 56-57 1.486432040596867 1106 58-59 -8.635851019731188 1106 60-61 -9.948930480432782 1106 62-63 -3.89465769846327 1106 64-65 -5.605097852847901 1106 66-67 -6.988342370746601 1106 68-69 -4.161460951117137 1106 70-71 -4.0132656170576695 1106 72-73 -4.654987797322452 1106 74-75 -5.138333901604028 1106 76-77 -6.755490148371482 1106 78-79 -9.319960818049209 1106 80-81 -4.901042235024505 1106 82-83 -2.3929866291617437 1106 84-85 -1.9303245013909596 1106 86-87 -1.8783932955678324 1106 88-89 -1.846066612259591 1106 90-91 -4.430585860308437 1106 92-93 -6.857728371674122 1106 94-95 -7.353254702870225 1106 96-97 -7.217179790741607 1106 98-99 -6.779719670221125 1106 100-101 -7.29176152598934 1107 1 0.03155545367911827 1107 2 -0.11105753772689653 1107 3 0.0017192702328507892 1107 4 0.03469320144051835 1107 5 -0.11272936028493064 1107 6 -0.12880844548271142 1107 7 0.004783433746268884 1107 8 0.013752425547615132 1107 9 0.0017058311092839062 1107 10-11 0.023014165317576385 1107 12-13 -0.0024773223062979355 1107 14-15 0.055388838973478016 1107 16-17 -0.008478310777761067 1107 18-19 0.021442741653409314 1107 20-21 -0.007319592132184027 1107 22-23 0.0760319787883148 1107 24-25 0.0074912627011229915 1107 26-27 -0.026797633181168123 1107 28-29 -0.016850395687342257 1107 30-31 -0.09158351761070804 1107 32-33 -0.01824857491245524 1107 34-35 -0.08577318311434823 1107 36-37 -0.14545318508300653 1107 38-39 -0.25575934364538 1107 40-41 -0.2250085560751316 1107 42-43 -0.3663985410126358 1107 44-45 -0.22957133365866866 1107 46-47 -0.1125501356868952 1107 48-49 -0.20182006355825877 1107 50-51 -0.04476829713600239 1107 52-53 -0.3297579111393887 1107 54-55 -0.3737102890481978 1107 56-57 0.6967651836432367 1107 58-59 -9.069717817701612 1107 60-61 -10.600494268534728 1107 62-63 -4.455241175230437 1107 64-65 -6.140439058657922 1107 66-67 -7.470230040495807 1107 68-69 -4.228439881002998 1107 70-71 -4.29174808421161 1107 72-73 -2.691218494117697 1107 74-75 -4.923007234638423 1107 76-77 -6.920100522863139 1107 78-79 -7.893936027853529 1107 80-81 -3.1386635214104217 1107 82-83 -1.9839790130838146 1107 84-85 -1.704905367029962 1107 86-87 -1.6713855035750598 1107 88-89 -1.5432645647487853 1107 90-91 -1.2673970329705817 1107 92-93 -4.260244169516923 1107 94-95 -6.199050683043588 1107 96-97 -6.474318354724595 1107 98-99 -6.239499630071148 1107 100-101 -6.817160740581304 1108 1 -0.13021409573383735 1108 2 -0.21534210899530137 1108 3 -0.1796279705239634 1108 4 -0.035928024901799915 1108 5 -0.11100739342304422 1108 6 -0.21224747623254814 1108 7 -0.18302583516167914 1108 8 -0.20550085443382216 1108 9 -0.3123001231554099 1108 10-11 -0.23131851040455587 1108 12-13 -0.18696606355611323 1108 14-15 -0.31547492655208487 1108 16-17 -0.34224519412066456 1108 18-19 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13.581316071552385 2316 98-99 13.146313971025794 2316 100-101 12.106255635692483 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 18.0 4 44.0 5 93.0 6 242.0 7 599.0 8 1506.0 9 2552.0 10 4219.0 11 6697.0 12 9900.0 13 13747.0 14 17637.0 15 24259.0 16 33821.0 17 45251.0 18 51459.0 19 53683.0 20 56313.0 21 56679.0 22 55722.0 23 48071.0 24 47712.0 25 54877.0 26 77241.0 27 115051.0 28 163985.0 29 221159.0 30 269710.0 31 286931.0 32 279486.0 33 250082.0 34 218610.0 35 192302.0 36 152996.0 37 95025.0 38 34110.0 39 2053.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.78428587737706 20.56473095364944 14.983337501223184 23.667645667750318 2 15.29381190263059 21.442740013622615 39.91320255398512 23.350245529761672 3 18.57249811640706 25.5895187309645 30.778859734999365 25.05912341762907 4 12.77353691296142 17.94307035291272 38.218906980125304 31.064485754000554 5 12.971181086667627 38.0412241039817 35.4424980586596 13.545096750691076 6 30.636168220870395 38.19726860526577 17.770982429775493 13.395580744088342 7 26.818099056879376 33.10263723213594 22.268503358194252 17.81076035279043 8 24.978658601831842 36.4057586616523 20.368339114392274 18.247243622123584 9 25.543133399731776 16.62887027981102 20.68479172130045 37.143204599156746 10-11 24.014502806534583 27.04326384142638 28.7220212755839 20.220212076455137 12-13 24.926660515978426 24.46306597768088 28.695236568242066 21.915036938098623 14-15 22.064683258702463 25.867019133629682 26.781068964088185 25.287228643579674 16-17 21.53366482734819 28.78386218834966 27.44445697530168 22.23801600900048 18-19 21.429313899299522 27.885118717429858 29.30240070873441 21.38316667453621 20-21 22.389246848678123 27.140976220207317 29.14070854297986 21.329068388134697 22-23 21.857875234897598 27.078291725490146 29.028162574273843 22.035670465338423 24-25 21.778695701281574 27.170492307248097 29.264286387603956 21.786525603866373 26-27 21.79719281189455 27.30254458851386 29.123875977445074 21.776386622146514 28-29 21.69970853404173 27.469239810581097 29.106357898455705 21.724693756921468 30-31 21.985434907571864 27.283847396751327 29.00139155202896 21.72932614364785 32-33 21.845393437819798 27.31652094895563 28.908089562252385 21.929996050972182 34-35 22.017971517948336 27.336950419937267 28.981471872177778 21.663606189936623 36-37 22.066728691786842 27.12721678595621 29.03306102129257 21.772993500964382 38-39 21.78448179270756 27.484881876563477 28.962272828591672 21.76836350213729 40-41 22.045366779445665 27.349505332842018 28.91826535163531 21.686862536077008 42-43 21.849629752316833 27.289786279018436 28.877788846078108 21.98279512258662 44-45 21.99417877918964 27.399306602377155 28.72874665208386 21.877767966349353 46-47 22.07288186516146 27.4015429657977 28.496646388170177 22.028928780870665 48-49 21.91514244265329 27.505609357476075 28.51747011346579 22.06177808640485 50-51 21.97033947296472 27.684178739854374 28.352749503855577 21.99273228332533 52-53 22.118178399759017 27.73532085668766 28.120000054398048 22.026500689155274 54-55 22.12866537985876 27.52562680893622 28.279915394306453 22.065792416898567 56-57 21.347178174406462 27.573889012828236 29.206244219880773 21.87268859288453 58-59 21.335135058630865 26.882741374864377 30.309891458075366 21.472232108429388 60-61 22.077502082696228 27.470759013221173 28.719710835194057 21.73202806888854 62-63 21.93234748936247 27.59911872947845 28.434868377583417 22.033665403575664 64-65 21.57619616974682 27.60794619640272 28.717664946461174 22.098192687389286 66-67 22.182980128517134 26.7718636439115 29.52012038936422 21.52503583820714 68-69 22.684562514144915 25.968544155399414 30.237326767410533 21.10956656304514 70-71 22.587621479193455 25.94708038474985 30.113177034166615 21.35212110189008 72-73 21.812087678971924 26.196524745193106 30.450976451558432 21.54041112427654 74-75 22.158936485309695 26.625168479496807 29.538910443787476 21.676984591406022 76-77 20.092015131024805 27.37662962488127 30.035656337150552 22.49569890694337 78-79 20.784305858056694 27.474607908800063 29.346851318962113 22.394234914181126 80-81 21.6010035841563 27.630104737710486 28.107442778998216 22.661448899135 82-83 21.990028997874035 27.61653033380081 27.939883889611394 22.45355677871376 84-85 22.01234884318552 27.564895288922948 27.816488574356235 22.606267293535296 86-87 21.807207879248626 27.492837571683733 28.053789717762616 22.646164831305025 88-89 21.93897733250154 27.438018466014036 27.915456017403116 22.707548184081304 90-91 21.351191933694846 27.475860443684923 28.427633095771874 22.745314526848347 92-93 20.35752017702058 27.648653373069166 29.055114283156186 22.93871216675407 94-95 20.172320335404766 27.076765304896643 29.953572594326953 22.797341765371634 96-97 20.31169185672731 26.926517814382798 29.81369411532863 22.948096213561254 98-99 20.239671154892477 27.29501598227068 29.125820548751914 23.339492314084925 100-101 20.588845575812783 27.310456895410468 28.751729417276405 23.348968111500344 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 377.0 1 302.5 2 238.0 3 279.0 4 318.0 5 354.0 6 464.5 7 657.5 8 942.5 9 1214.0 10 1440.0 11 1656.5 12 1837.0 13 2091.0 14 2400.0 15 2707.0 16 3108.0 17 3564.5 18 4016.5 19 4510.5 20 5172.5 21 6139.5 22 7503.0 23 9205.0 24 11393.5 25 14485.0 26 18483.0 27 23329.0 28 28273.0 29 33642.0 30 39755.5 31 46228.5 32 53491.0 33 61317.0 34 68737.5 35 75801.5 36 82428.0 37 88471.0 38 93485.5 39 97762.5 40 101712.5 41 104619.5 42 107323.5 43 109916.5 44 111636.5 45 113557.5 46 115407.0 47 116075.0 48 115657.5 49 113277.5 50 109718.0 51 105400.0 52 100312.0 53 94213.0 54 86983.0 55 79140.0 56 70896.0 57 62285.5 58 53526.5 59 44937.5 60 36099.5 61 27672.5 62 20552.0 63 14866.0 64 10388.0 65 7168.0 66 4851.5 67 3128.0 68 2015.0 69 1269.0 70 789.5 71 466.5 72 266.0 73 157.5 74 83.0 75 36.5 76 17.0 77 10.0 78 6.0 79 4.5 80 4.0 81 2.5 82 2.0 83 1.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024050187441997606 2 0.007099560982171609 3 6.793838260451301E-5 4 0.0 5 0.13129092438322137 6 0.0 7 0.0 8 0.0011889216955789778 9 0.0 10-11 0.0 12-13 0.0 14-15 7.473222086496432E-4 16-17 0.0 18-19 1.6984595651128252E-4 20-21 0.0 22-23 2.7175353041805205E-4 24-25 5.095378695338476E-5 26-27 2.887381260691803E-4 28-29 0.0035327958954346765 30-31 0.012500662399230394 32-33 0.002768489091133905 34-35 0.004432979464944474 36-37 0.10530449303699516 38-39 0.10481193976311245 40-41 0.11659924914499545 42-43 0.11940170742743161 44-45 0.11534238906681195 46-47 0.10899015029329001 48-49 0.10944873437587045 50-51 0.1076143980455486 52-53 0.08702906811638117 54-55 0.0746642824823598 56-57 0.5701728760083754 58-59 1.5962462684843355 60-61 0.5281699709631352 62-63 0.004738702186664783 64-65 0.12210225813596101 66-67 1.909527135269396 68-69 2.8888419359178 70-71 2.7884799602152834 72-73 2.6411725621330477 74-75 2.01165550891963 76-77 1.4354021476681509 78-79 0.9469591459330046 80-81 0.05399402957493672 82-83 2.547689347669238E-4 84-85 1.868305521624108E-4 86-87 3.3969191302256503E-4 88-89 1.1889216955789778E-4 90-91 0.2541235201321809 92-93 0.40700186558798634 94-95 2.019417469132196 96-97 2.4360156312630696 98-99 1.4491426855499137 100-101 0.9552815978020576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2943844.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.584656890518104 #Duplication Level Percentage of deduplicated Percentage of total 1 73.47906201824276 40.843084509211245 2 14.314707584976855 15.91356219198071 3 5.207909075831236 8.684395172912835 4 2.4297205456319113 5.402207314751689 5 1.3492533140800238 3.749889126076629 6 0.7980987152377942 2.661722595075366 7 0.514691842374581 2.0026278623917793 8 0.3525342136716564 1.5676394647286092 9 0.2726388025714708 1.3639080866379218 >10 1.190388381275393 11.285739481312259 >50 0.05991267919511304 2.2867258154339294 >100 0.029616392817143684 2.9599267175823236 >500 0.0010387241499475463 0.3644910768279141 >1k 2.44405682261348E-4 0.1889362523141306 >5k 1.83304261696011E-4 0.7251443327627185 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9165 0.3113276382851809 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6960 0.23642557146370527 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5226 0.1775229937455925 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.3969191302256506E-5 0.0 3 0.0 0.0 0.0 3.3969191302256506E-5 0.0 4 0.0 0.0 0.0 3.3969191302256506E-5 0.0 5 0.0 0.0 0.0 3.3969191302256506E-5 0.0 6 0.0 0.0 0.0 3.3969191302256506E-5 0.0 7 0.0 0.0 0.0 3.3969191302256506E-5 0.0 8 0.0 0.0 0.0 3.3969191302256506E-5 0.0 9 0.0 0.0 0.0 6.793838260451301E-5 0.0 10-11 0.0 3.3969191302256506E-5 0.0 1.1889216955789778E-4 0.0 12-13 0.0 3.3969191302256506E-5 0.0 1.6984595651128252E-4 3.3969191302256506E-5 14-15 0.0 3.3969191302256506E-5 0.0 2.0381514781353904E-4 5.095378695338476E-5 16-17 0.0 3.3969191302256506E-5 0.0 2.0381514781353904E-4 6.793838260451301E-5 18-19 0.0 3.3969191302256506E-5 0.0 2.3778433911579556E-4 6.793838260451301E-5 20-21 0.0 3.3969191302256506E-5 0.0 3.3969191302256503E-4 1.0190757390676952E-4 22-23 0.0 3.3969191302256506E-5 0.0 5.095378695338476E-4 1.3587676520902602E-4 24-25 0.0 3.3969191302256506E-5 0.0 7.47322208649643E-4 1.3587676520902602E-4 26-27 0.0 3.3969191302256506E-5 0.0 0.0011379679086255929 1.3587676520902602E-4 28-29 0.0 3.3969191302256506E-5 0.0 0.0022759358172511858 1.3587676520902602E-4 30-31 0.0 3.3969191302256506E-5 0.0 0.006556053921335505 1.3587676520902602E-4 32-33 0.0 3.3969191302256506E-5 0.0 0.01554090502078235 1.3587676520902602E-4 34-35 0.0 3.3969191302256506E-5 0.0 0.02800759822871049 1.3587676520902602E-4 36-37 0.0 3.3969191302256506E-5 0.0 0.04813434407529747 1.3587676520902602E-4 38-39 0.0 3.3969191302256506E-5 0.0 0.07901233896904863 1.5286136086015427E-4 40-41 0.0 3.3969191302256506E-5 0.0 0.11354202192779236 1.6984595651128252E-4 42-43 0.0 3.3969191302256506E-5 0.0 0.15095908614722792 1.6984595651128252E-4 44-45 0.0 3.3969191302256506E-5 0.0 0.1973100476791569 1.6984595651128252E-4 46-47 3.3969191302256506E-5 3.3969191302256506E-5 0.0 0.24880734169337776 1.6984595651128252E-4 48-49 3.3969191302256506E-5 3.3969191302256506E-5 0.0 0.2965680246643504 1.6984595651128252E-4 50-51 3.3969191302256506E-5 3.3969191302256506E-5 0.0 0.3434964624484178 1.6984595651128252E-4 52-53 5.095378695338476E-5 3.3969191302256506E-5 0.0 0.388675486880419 1.6984595651128252E-4 54-55 6.793838260451301E-5 3.3969191302256506E-5 0.0 0.44086914931633603 1.6984595651128252E-4 56-57 6.793838260451301E-5 3.3969191302256506E-5 0.0 0.4844686063527823 1.6984595651128252E-4 58-59 6.793838260451301E-5 3.3969191302256506E-5 0.0 0.5147181712074418 1.6984595651128252E-4 60-61 6.793838260451301E-5 3.3969191302256506E-5 0.0 0.5390570967755084 1.6984595651128252E-4 62-63 1.0190757390676952E-4 3.3969191302256506E-5 0.0 0.5602199029568142 2.3778433911579553E-4 64-65 1.0190757390676952E-4 3.3969191302256506E-5 0.0 0.5822489235163277 2.3778433911579553E-4 66-67 1.0190757390676952E-4 3.3969191302256506E-5 0.0 0.6025794845107282 2.3778433911579553E-4 68-69 1.0190757390676952E-4 3.3969191302256506E-5 0.0 0.6296868991699288 2.3778433911579553E-4 70-71 1.0190757390676952E-4 3.3969191302256506E-5 0.0 0.6721144191064472 2.3778433911579553E-4 72-73 1.0190757390676952E-4 3.3969191302256506E-5 0.0 0.7336156399591827 2.3778433911579553E-4 74-75 1.0190757390676952E-4 3.3969191302256506E-5 0.0 0.8168741278410132 2.3778433911579553E-4 76-77 1.0190757390676952E-4 3.3969191302256506E-5 0.0 0.9524791395196213 2.3778433911579553E-4 78-79 1.0190757390676952E-4 3.3969191302256506E-5 0.0 1.1624766801501711 2.3778433911579553E-4 80-81 1.0190757390676952E-4 3.3969191302256506E-5 0.0 1.4187232747387428 2.3778433911579553E-4 82-83 1.0190757390676952E-4 3.3969191302256506E-5 0.0 1.677381681909775 2.3778433911579553E-4 84-85 1.0190757390676952E-4 3.3969191302256506E-5 0.0 1.872670562706448 2.3778433911579553E-4 86-87 1.0190757390676952E-4 6.793838260451301E-5 0.0 2.024105217531907 2.3778433911579553E-4 88-89 1.0190757390676952E-4 1.0190757390676952E-4 0.0 2.1686271419273577 2.3778433911579553E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5860 0.0 40.08576 1 GTATCAA 9390 0.0 33.939877 1 ATCAACG 11425 0.0 27.4334 3 TCAACGC 11490 0.0 27.237247 4 TATCAAC 11710 0.0 26.927849 2 CAACGCA 11830 0.0 26.454435 5 AACGCAG 12295 0.0 25.455395 6 ACGCAGA 13805 0.0 22.3647 7 CGCAGAG 13655 0.0 22.162989 8 TACATGG 8145 0.0 22.014948 2 GTACATG 8575 0.0 21.574741 1 ACATGGG 8045 0.0 20.708033 3 GCAGAGT 14785 0.0 19.451668 9 AGAGTAC 11775 0.0 19.1561 10-11 GAGTACT 8085 0.0 19.064287 12-13 AGTACTT 8890 0.0 17.760872 12-13 GGACCGA 375 2.0008883E-10 17.544092 6 GTACTTT 8955 0.0 17.317097 14-15 CAGAGTA 13870 0.0 17.126614 10-11 CATGGGG 4540 0.0 16.68905 4 >>END_MODULE