FastQCFastQC Report
Fri 27 May 2016
SRR939568_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939568_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2712855
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33060.1218642352798067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48100.044.2022861
GTATCAA74850.043.2374881
TATCAAC100300.030.4218392
GTACATG66700.029.5206411
ATCAACG107300.028.3496743
TCAACGC107450.028.3069574
CAACGCA109850.027.7089795
AACGCAG114250.026.6831576
TACATGG76950.025.4123972
ACGCAGA118550.025.1978237
ACATGGG78450.023.6023373
CGCAGAG124950.023.3402278
GCAGAGT130100.020.4217039
GTGTAGG11400.020.2121071
GAGTACT66450.019.95667812-13
AGAGTAC100350.019.72747210-11
GAGTACA54450.018.850461
CATGGGA41350.018.731914
GTACTTT74100.017.86634314-15
AGTACTT73850.017.86110112-13
Produced by FastQC (version 0.11.5)