##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939568_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2712855 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 14.80617209544926 2.0 2.0 31.0 2.0 33.0 2 22.399502737890526 16.0 16.0 31.0 16.0 34.0 3 27.184174237104454 28.0 25.0 31.0 16.0 34.0 4 32.485888851412994 35.0 32.0 35.0 26.0 37.0 5 33.09666274091317 35.0 33.0 37.0 28.0 37.0 6 33.5018233558373 35.0 33.0 37.0 28.0 37.0 7 33.677141609116596 35.0 33.0 37.0 28.0 37.0 8 33.61426246518889 35.0 33.0 37.0 28.0 37.0 9 35.01379653538431 37.0 34.0 39.0 28.0 39.0 10-11 34.868706215407755 37.0 34.0 39.0 27.0 39.0 12-13 34.869700923934374 37.0 34.0 39.0 27.0 39.0 14-15 35.610617965206394 38.0 34.0 40.0 27.0 41.0 16-17 35.22261012844402 38.0 33.5 40.0 27.0 41.0 18-19 35.460269900160526 38.0 34.0 40.0 27.0 41.0 20-21 35.44906565223722 38.0 34.0 40.0 27.0 41.0 22-23 35.268177620993384 38.0 34.0 40.0 26.5 41.0 24-25 35.106154033297024 38.0 34.0 40.0 25.0 41.0 26-27 35.20688149569365 38.0 34.0 40.0 25.5 41.0 28-29 34.96165515665231 38.0 34.0 40.0 25.0 41.0 30-31 34.84353863365348 38.0 34.0 40.0 24.5 41.0 32-33 34.56112195454604 38.0 33.0 40.0 24.0 41.0 34-35 34.30706267013902 38.0 33.0 40.0 24.0 41.0 36-37 34.0619262732435 38.0 33.0 40.0 23.0 41.0 38-39 33.87649468917432 38.0 33.0 40.0 22.0 41.0 40-41 33.514671812536974 38.0 32.0 40.0 19.0 41.0 42-43 33.28420372633259 37.0 31.0 40.0 18.5 41.0 44-45 32.96364402078254 37.0 31.0 40.0 17.0 41.0 46-47 32.657265132120955 37.0 31.0 40.0 15.0 41.0 48-49 32.30576790871609 37.0 30.5 39.5 13.5 40.5 50-51 31.66360844940109 36.0 30.0 38.5 10.5 40.0 52-53 31.72500852423001 36.0 30.0 39.0 9.0 40.0 54-55 31.857949466521433 36.0 30.0 39.0 8.5 40.0 56-57 31.57581017046617 36.0 30.0 39.0 8.0 40.0 58-59 31.173707772807614 36.0 29.0 39.0 7.0 40.0 60-61 30.612499377961594 35.0 28.0 38.5 4.0 40.0 62-63 30.213072574833525 35.0 28.0 38.0 2.0 40.0 64-65 29.647652749594062 34.0 27.5 38.0 2.0 40.0 66-67 29.056072108535105 34.0 26.0 37.5 2.0 39.5 68-69 28.713909884604966 34.0 26.0 37.0 2.0 39.0 70-71 28.199719115101985 33.5 26.0 36.5 2.0 39.0 72-73 27.596147416651462 33.0 25.0 36.0 2.0 39.0 74-75 27.035788311575814 32.0 24.0 35.5 2.0 38.0 76-77 26.3949322761445 32.0 22.5 35.0 2.0 37.5 78-79 25.726896572061534 31.0 20.5 35.0 2.0 36.5 80-81 24.984942615805117 31.0 18.5 34.0 2.0 36.0 82-83 24.286013074786524 30.5 15.5 34.0 2.0 35.5 84-85 23.580350958676377 30.0 9.0 34.0 2.0 35.0 86-87 22.886690405495315 29.0 4.5 34.0 2.0 35.0 88-89 22.08288187168131 29.0 2.0 34.0 2.0 35.0 90-91 21.169746447930315 28.5 2.0 33.0 2.0 34.5 92-93 20.13667427857368 26.5 2.0 32.5 2.0 34.0 94-95 19.13503670487365 25.5 2.0 32.0 2.0 34.0 96-97 18.134102817880056 24.5 2.0 32.0 2.0 34.0 98-99 17.00342812277103 21.0 2.0 31.5 2.0 34.0 100-101 15.382213203433285 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.61046311886453 1101 2 7.935416953446289 1101 3 3.5394482901063924 1101 4 2.0996154003379743 1101 5 1.4371946710814143 1101 6 1.2047300049667413 1101 7 0.7897487033562385 1101 8 0.9439126590846172 1101 9 0.8288714327197084 1101 10-11 0.954056524637096 1101 12-13 0.4455898176833415 1101 14-15 0.7592553126149326 1101 16-17 0.6851738999507759 1101 18-19 0.8088231075373002 1101 20-21 0.7039383485687054 1101 22-23 0.7634990544793894 1101 24-25 1.0544718852342143 1101 26-27 1.0330918617686322 1101 28-29 1.0072915450566597 1101 30-31 0.8679651158822921 1101 32-33 0.940563833034652 1101 34-35 0.9105741234590141 1101 36-37 0.7992791476297825 1101 38-39 0.7243360183661096 1101 40-41 0.7769205804648109 1101 42-43 0.702498715738777 1101 44-45 0.5576174227530402 1101 46-47 0.45632397427129234 1101 48-49 0.5141951322653782 1101 50-51 0.5212759157794977 1101 52-53 0.6784007850686145 1101 54-55 0.695564036684047 1101 56-57 0.45638720504597075 1101 58-59 0.5072863553061033 1101 60-61 0.6294746090054346 1101 62-63 0.2598656783801019 1101 64-65 0.23937022117769757 1101 66-67 0.2165254105104104 1101 68-69 0.06381454504972695 1101 70-71 -0.22256006733317335 1101 72-73 -0.15642925586092815 1101 74-75 -0.1850959184170584 1101 76-77 -0.15020922214632293 1101 78-79 -0.29688399432303214 1101 80-81 -0.46621313523040087 1101 82-83 -0.4240721854084484 1101 84-85 -0.6549981974222625 1101 86-87 -0.7299312118520547 1101 88-89 -0.5978114568769044 1101 90-91 -0.7318457994109657 1101 92-93 -0.5401048725592013 1101 94-95 -0.6327353890842282 1101 96-97 -1.2640535086506723 1101 98-99 -1.5862164635308034 1101 100-101 -1.5868572756661408 1102 1 14.846898961409341 1102 2 8.030284611736043 1102 3 3.7301541550118174 1102 4 2.1420238432251537 1102 5 1.5529594159186146 1102 6 1.1973034102582787 1102 7 1.0458146817449574 1102 8 0.866678785489654 1102 9 1.077817386426716 1102 10-11 1.01875717877639 1102 12-13 0.7584381623695222 1102 14-15 0.8031841785091842 1102 16-17 0.9912648147557377 1102 18-19 1.0332980973799195 1102 20-21 0.9212127428130472 1102 22-23 0.8978536511400463 1102 24-25 0.7871048729496408 1102 26-27 0.913330717306259 1102 28-29 1.0565630484021256 1102 30-31 1.0725165972574189 1102 32-33 1.0593262370530638 1102 34-35 1.0575962244362955 1102 36-37 0.9742359402561718 1102 38-39 0.8963467089815964 1102 40-41 0.8700910116265348 1102 42-43 0.7049200540807519 1102 44-45 0.7015668396229557 1102 46-47 0.6655561175172409 1102 48-49 0.6652689387168849 1102 50-51 0.46212787645144715 1102 52-53 0.5548681103524551 1102 54-55 0.4583386607788711 1102 56-57 0.33884928382286006 1102 58-59 0.32510144253631523 1102 60-61 0.3244445702779899 1102 62-63 0.05832258870472273 1102 64-65 0.08346924627474195 1102 66-67 0.12918340448048227 1102 68-69 -0.2175617931258742 1102 70-71 -0.016599955739632577 1102 72-73 -0.05021768495297607 1102 74-75 -0.08337285108175152 1102 76-77 -0.18883149952718625 1102 78-79 -0.07335546882934807 1102 80-81 -0.06149263466162935 1102 82-83 -0.15965882384396934 1102 84-85 -0.3651142072177116 1102 86-87 -0.39394961673482953 1102 88-89 -0.3752135280372997 1102 90-91 -0.2737002594759339 1102 92-93 -0.18153209622406408 1102 94-95 -0.1241494256162845 1102 96-97 -0.8636651455353679 1102 98-99 -1.131317125311245 1102 100-101 -0.99498465761768 1103 1 14.748463245234703 1103 2 7.912360653893177 1103 3 3.668983256076711 1103 4 2.1458406248091464 1103 5 1.3978500563381218 1103 6 0.9688834643091937 1103 7 0.6727242819100709 1103 8 0.7762511350275076 1103 9 0.7864350568193217 1103 10-11 0.6928917916802391 1103 12-13 0.308213541467687 1103 14-15 0.5232810170465854 1103 16-17 0.711771026096109 1103 18-19 0.6105345104759792 1103 20-21 0.5104900778706707 1103 22-23 0.5177067606802339 1103 24-25 0.699487402506719 1103 26-27 0.703904177854902 1103 28-29 0.8680512062204855 1103 30-31 0.8003933331109749 1103 32-33 0.766466456610722 1103 34-35 0.6857426101844055 1103 36-37 0.8250698433386177 1103 38-39 0.636789234733186 1103 40-41 0.6096053435043203 1103 42-43 0.649411036994735 1103 44-45 0.6467614761337686 1103 46-47 0.5640858826086088 1103 48-49 0.5222375885404702 1103 50-51 0.48116179694886796 1103 52-53 0.7921469160078054 1103 54-55 0.5302413352672275 1103 56-57 0.4760079181019776 1103 58-59 0.44834502963375655 1103 60-61 0.5683166766512251 1103 62-63 0.3517380760982185 1103 64-65 0.31905815454943465 1103 66-67 0.34372196413321277 1103 68-69 0.19280571924198142 1103 70-71 0.041692836719040116 1103 72-73 0.06868546199714842 1103 74-75 -0.12899746005110657 1103 76-77 -0.0926145799134126 1103 78-79 -0.2675352397257029 1103 80-81 -0.4308067669122231 1103 82-83 -0.43659801333822656 1103 84-85 -0.522385522822173 1103 86-87 -0.5846725961142099 1103 88-89 -0.7505539665721237 1103 90-91 -0.5852013172742936 1103 92-93 -0.49904615720226886 1103 94-95 -0.34526314539001746 1103 96-97 -0.7789564190213945 1103 98-99 -1.030022047943616 1103 100-101 -0.8555600612258054 1104 1 14.930516154254615 1104 2 8.149475062327383 1104 3 3.754088358250339 1104 4 2.1583015775619785 1104 5 1.6338353485416874 1104 6 1.2126424126366686 1104 7 0.9069898682695694 1104 8 0.8766774008091076 1104 9 1.023028835291278 1104 10-11 0.8786590700871102 1104 12-13 0.7218215071061067 1104 14-15 0.8407749972626419 1104 16-17 1.0264414022512582 1104 18-19 0.7622582837416303 1104 20-21 0.7379386656618934 1104 22-23 0.8158219175200045 1104 24-25 0.9368458562357347 1104 26-27 0.8268842962982106 1104 28-29 0.8824685887533406 1104 30-31 0.8969165011442968 1104 32-33 0.8417487356683822 1104 34-35 0.973005886248437 1104 36-37 1.2326336205089916 1104 38-39 1.02980059097424 1104 40-41 1.1814229928600426 1104 42-43 0.918301769781408 1104 44-45 0.6894596409131566 1104 46-47 0.7518828043839534 1104 48-49 0.6616932048361903 1104 50-51 0.5573315873953106 1104 52-53 0.5978746590769184 1104 54-55 0.7035435934996883 1104 56-57 0.6051095060233607 1104 58-59 0.5936121778831378 1104 60-61 0.32002336674623777 1104 62-63 0.2966497317014962 1104 64-65 0.44323409493730637 1104 66-67 0.44053635955166826 1104 68-69 0.5784802604368302 1104 70-71 0.5077038350269945 1104 72-73 0.42623134251257255 1104 74-75 0.3242109220912859 1104 76-77 0.07978064330668388 1104 78-79 -0.10480339669563676 1104 80-81 -0.04176602761767967 1104 82-83 -0.14174655557432914 1104 84-85 -0.10116090353283269 1104 86-87 -9.650599955079997E-4 1104 88-89 -0.17424832424490688 1104 90-91 -0.15496329176342627 1104 92-93 0.1457532375653976 1104 94-95 0.36083554652720196 1104 96-97 -0.2141501909854746 1104 98-99 -0.5500589801315634 1104 100-101 -0.47359500851202085 1105 1 14.71206195195219 1105 2 8.007493735046904 1105 3 3.773654688833137 1105 4 2.2568371673390075 1105 5 1.5069275291575082 1105 6 1.2084994683078776 1105 7 0.9451834592672057 1105 8 0.9211509321142302 1105 9 0.928437319318931 1105 10-11 0.9114734903424377 1105 12-13 0.805654368082763 1105 14-15 1.0063253555012963 1105 16-17 1.2107071175163213 1105 18-19 0.9338619137818469 1105 20-21 0.9117103815288985 1105 22-23 0.8390472358382439 1105 24-25 0.9922965597545783 1105 26-27 0.825101881343322 1105 28-29 1.1639428536743779 1105 30-31 1.062762343569453 1105 32-33 0.7019832524015115 1105 34-35 0.9268650508041247 1105 36-37 0.992649254261174 1105 38-39 0.9600195053062919 1105 40-41 1.085905887695887 1105 42-43 0.8817311211060925 1105 44-45 0.8682172278811251 1105 46-47 0.742672894089921 1105 48-49 0.6377681298025379 1105 50-51 0.7553225039344795 1105 52-53 0.7510593045585914 1105 54-55 0.6608479834907399 1105 56-57 0.7252003406316447 1105 58-59 0.5700422063917436 1105 60-61 0.6600351129611148 1105 62-63 0.5038316433666168 1105 64-65 0.5828843628847693 1105 66-67 0.48007444278676914 1105 68-69 0.31632716043933584 1105 70-71 0.4771765825544385 1105 72-73 0.5914190729432107 1105 74-75 0.4393293143229009 1105 76-77 0.45550056923446647 1105 78-79 0.4226443410084606 1105 80-81 0.3983891499131964 1105 82-83 0.5890694405966528 1105 84-85 0.5390473618730987 1105 86-87 0.33415319638130825 1105 88-89 0.40371686348552416 1105 90-91 0.7334694559312247 1105 92-93 0.9385893527869875 1105 94-95 0.687676953815707 1105 96-97 0.3372922734623707 1105 98-99 0.10194430337440963 1105 100-101 0.19129081075511678 1106 1 14.713152237200411 1106 2 7.965555022175927 1106 3 3.3689129497473793 1106 4 2.1561723432350206 1106 5 1.550603029159717 1106 6 1.097579263034369 1106 7 0.8023965923680692 1106 8 0.8311140733206059 1106 9 0.9526843713640574 1106 10-11 0.9228661740997168 1106 12-13 0.6168432346352759 1106 14-15 0.9526990906783155 1106 16-17 1.0390592518611825 1106 18-19 0.8955503220634 1106 20-21 0.9446136866270578 1106 22-23 0.7762384253597077 1106 24-25 0.9210743383581672 1106 26-27 0.8945090072331325 1106 28-29 0.9743784195073317 1106 30-31 0.9112708059904264 1106 32-33 0.7882556541710883 1106 34-35 0.7257076917934029 1106 36-37 0.8824582755368127 1106 38-39 0.9678815800215332 1106 40-41 1.1416180959069777 1106 42-43 0.7843464700431539 1106 44-45 0.797233109185882 1106 46-47 0.7945151390133134 1106 48-49 0.8610083429521254 1106 50-51 0.7905978957483342 1106 52-53 0.9191530255733191 1106 54-55 0.8150363128714986 1106 56-57 0.7379898648452468 1106 58-59 0.6650735130461563 1106 60-61 0.7847707683370508 1106 62-63 0.7304138037708228 1106 64-65 0.8992749198210426 1106 66-67 0.619744162402931 1106 68-69 0.7729084515882612 1106 70-71 0.9351753414690158 1106 72-73 0.8710154648982815 1106 74-75 0.826675122492798 1106 76-77 0.8157635281126616 1106 78-79 0.6558410278424951 1106 80-81 0.7185664607561897 1106 82-83 0.7899522894359627 1106 84-85 0.3865393772732446 1106 86-87 0.6700344063458843 1106 88-89 0.7236335593158678 1106 90-91 0.8404400969687522 1106 92-93 1.022780981460091 1106 94-95 1.1724729572664465 1106 96-97 0.9701033857978381 1106 98-99 0.8275834241275426 1106 100-101 0.8088288816051303 1107 1 14.711942478589911 1107 2 7.986812691716466 1107 3 3.6257707545699382 1107 4 2.102154327714196 1107 5 1.4036310321896153 1107 6 1.1980984034356652 1107 7 0.8958962345506052 1107 8 0.8333049970475201 1107 9 0.7759295186009325 1107 10-11 0.7312252868518812 1107 12-13 0.43229441151041215 1107 14-15 0.609545716429146 1107 16-17 0.8791171346103681 1107 18-19 0.7014953110063615 1107 20-21 0.6307567260096434 1107 22-23 0.5507121482215851 1107 24-25 0.7293666599702533 1107 26-27 0.7955078670985714 1107 28-29 0.9322195359152374 1107 30-31 0.8389227084374511 1107 32-33 0.8283654226313786 1107 34-35 0.8200946319354685 1107 36-37 0.7054280863773386 1107 38-39 0.5665741516264262 1107 40-41 0.8110454811896517 1107 42-43 0.6363079939881615 1107 44-45 0.5190631014367995 1107 46-47 0.700744518746987 1107 48-49 0.6006942873069292 1107 50-51 0.4819654078404696 1107 52-53 0.5559627451333391 1107 54-55 0.5963730705310155 1107 56-57 0.6200509849490174 1107 58-59 0.574953088511645 1107 60-61 0.7301630731260502 1107 62-63 0.490043277469713 1107 64-65 0.3784194680514368 1107 66-67 0.251531471519737 1107 68-69 0.37350892899782195 1107 70-71 0.26558092385724663 1107 72-73 0.3258710827195941 1107 74-75 0.4372255185326601 1107 76-77 0.214863689108725 1107 78-79 0.2495615819932233 1107 80-81 0.16501336822834034 1107 82-83 0.16816354861347804 1107 84-85 0.32184371388141386 1107 86-87 0.3456961943174619 1107 88-89 0.4045090331734009 1107 90-91 0.8886311030677767 1107 92-93 0.9419261115252091 1107 94-95 0.9207262000689092 1107 96-97 0.370980212911423 1107 98-99 0.18211281135750923 1107 100-101 0.16876088757078733 1108 1 14.32573137845445 1108 2 7.418271372769674 1108 3 3.174291487626757 1108 4 -0.21405634009137486 1108 5 0.22267946536494776 1108 6 0.47324002422733 1108 7 0.4545021540638814 1108 8 0.334532988617525 1108 9 0.5130633373664892 1108 10-11 0.3355991115914492 1108 12-13 0.18157757361490923 1108 14-15 0.20030890096239062 1108 16-17 0.40193028774510964 1108 18-19 0.32661611227522513 1108 20-21 0.2261552839492751 1108 22-23 -0.5256964284174117 1108 24-25 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2312 3 -3.1590273637469615 2312 4 -1.23456279490766 2312 5 -1.7659790914043327 2312 6 -0.6170218923565827 2312 7 -0.05172301971963833 2312 8 0.25047969500613476 2312 9 0.1741337610672531 2312 10-11 0.2738387259575603 2312 12-13 -0.3532670556469242 2312 14-15 0.16087452492796217 2312 16-17 -0.1447419648202697 2312 18-19 0.2036508276012725 2312 20-21 0.017640926120741085 2312 22-23 0.21940660038099935 2312 24-25 0.1461829007701425 2312 26-27 0.2383426296315463 2312 28-29 -0.6461962223127813 2312 30-31 -0.04482607645354619 2312 32-33 -8.583830900477096E-4 2312 34-35 -0.8104202271118837 2312 36-37 -0.1704576412993717 2312 38-39 0.19482673807275575 2312 40-41 0.24981874184789632 2312 42-43 0.18865011661001319 2312 44-45 0.4994798838281298 2312 46-47 0.34648469372268664 2312 48-49 0.3749860523395583 2312 50-51 0.3870085442104241 2312 52-53 0.22152091413764552 2312 54-55 0.10909352602330458 2312 56-57 0.20291230629327117 2312 58-59 0.07525860163482534 2312 60-61 0.2815196555451607 2312 62-63 0.12416744425210524 2312 64-65 0.16091712462691632 2312 66-67 -0.062128957587329836 2312 68-69 0.3023669001129434 2312 70-71 0.30167313327260814 2312 72-73 0.06125158542755216 2312 74-75 0.37973133369722234 2312 76-77 0.4231932613903524 2312 78-79 0.6101890122112472 2312 80-81 0.5669974676589753 2312 82-83 0.5180164336294482 2312 84-85 0.44028646488822076 2312 86-87 0.43328342178586965 2312 88-89 1.0063564328401142 2312 90-91 1.2743214585739544 2312 92-93 1.8660720634763273 2312 94-95 1.691693340049465 2312 96-97 1.5789166106339572 2312 98-99 1.4393683140654687 2312 100-101 1.3569591412855502 2313 1 -14.220445384206922 2313 2 -7.394296512715123 2313 3 -3.381087548116124 2313 4 -0.9375927240506776 2313 5 -0.38955886720015087 2313 6 -0.7663012045855808 2313 7 -0.1642562890114192 2313 8 0.027808104778053178 2313 9 -2.3432466898087654 2313 10-11 -0.4255087773032713 2313 12-13 -0.6956394646080426 2313 14-15 -0.8068064512062776 2313 16-17 -0.5900951701292527 2313 18-19 0.07166328485795503 2313 20-21 -1.0512545994900009 2313 22-23 -1.8555685027317637 2313 24-25 -1.1292896994343167 2313 26-27 -0.22320500935037302 2313 28-29 0.23444909178458317 2313 30-31 -0.9540515849669546 2313 32-33 -0.042630182187593846 2313 34-35 -1.0061696895153815 2313 36-37 -1.0637924361314006 2313 38-39 -0.6538646330627245 2313 40-41 -0.12411245461277787 2313 42-43 0.03431023664385435 2313 44-45 -0.029265885904308675 2313 46-47 -0.5897907319479927 2313 48-49 -0.1127585100040065 2313 50-51 -1.0986705679621735 2313 52-53 -0.04076592003586654 2313 54-55 -0.6310142576690154 2313 56-57 -0.19803036036164912 2313 58-59 -0.10575366614111203 2313 60-61 -1.5578352727165523 2313 62-63 -0.19058814763283394 2313 64-65 0.06937424469600728 2313 66-67 -1.2325494726224058 2313 68-69 -0.3264449675501879 2313 70-71 -0.08832624981337389 2313 72-73 -0.9697421749277879 2313 74-75 -0.4513383291151989 2313 76-77 0.2409517924299429 2313 78-79 0.2796126602882403 2313 80-81 0.13590015387888243 2313 82-83 0.2804614621837551 2313 84-85 0.363327542548884 2313 86-87 0.35108811121372696 2313 88-89 0.8933594865645098 2313 90-91 1.1393871599170673 2313 92-93 1.6973443164463298 2313 94-95 1.9511161508860049 2313 96-97 1.4384690835778748 2313 98-99 1.2915983480838698 2313 100-101 1.1586033273115675 2314 1 -14.220445384206922 2314 2 -7.26968490478389 2314 3 -3.320834531607229 2314 4 -1.1795853870173865 2314 5 -1.0551297441644962 2314 6 -5.962272972848787 2314 7 -1.7832344363252837 2314 8 -4.206309100021812 2314 9 -1.6106914626214248 2314 10-11 -0.2878963904855709 2314 12-13 -2.925799280657195 2314 14-15 -2.8494534401748197 2314 16-17 -2.629548826838736 2314 18-19 -1.2174470663318928 2314 20-21 -1.6205953824356882 2314 22-23 -1.1259567709332643 2314 24-25 -2.8508554567031084 2314 26-27 -4.384336153877104 2314 28-29 -1.804477447198039 2314 30-31 -1.9554577625571596 2314 32-33 -4.133972064710967 2314 34-35 -2.631400974705336 2314 36-37 -0.8507997711191777 2314 38-39 -1.222235160289543 2314 40-41 -2.4305778032559573 2314 42-43 -1.3318250432313938 2314 44-45 -2.5106788636938795 2314 46-47 -2.1321898727409803 2314 48-49 -1.2062276708043989 2314 50-51 -2.237607311789432 2314 52-53 -1.0214350691995584 2314 54-55 -0.3910067097204504 2314 56-57 -1.2644020789870325 2314 58-59 -1.6118446969917883 2314 60-61 -0.6269603107006212 2314 62-63 -3.066886850481165 2314 64-65 -1.333083520543017 2314 66-67 -2.4930030177377063 2314 68-69 -2.22143507066912 2314 70-71 -2.7811923588424783 2314 72-73 -2.6875036161154995 2314 74-75 -2.19046801041722 2314 76-77 -0.9370858136726028 2314 78-79 -0.6054659022423472 2314 80-81 -0.27482762654922865 2314 82-83 -0.3752274205130348 2314 84-85 -0.17131063207033037 2314 86-87 -0.04686571515982152 2314 88-89 0.24598476807844705 2314 90-91 0.564450246993939 2314 92-93 1.25299185702195 2314 94-95 1.3262695928846462 2314 96-97 1.0816281102857026 2314 98-99 1.344064715847832 2314 100-101 1.3013563762077744 2315 1 -14.220445384206922 2315 2 -7.4462915303834585 2315 3 -4.524923632514202 2315 4 -2.828983995640762 2315 5 -3.6853510020991145 2315 6 -2.7748387601944877 2315 7 -3.325519579136113 2315 8 -3.4721775240175035 2315 9 -2.5868575703654457 2315 10-11 -4.7100109764509455 2315 12-13 -4.64699258418424 2315 14-15 -3.4251658651344243 2315 16-17 -4.656629252618558 2315 18-19 -1.820421397346422 2315 20-21 -3.4168684065893373 2315 22-23 -4.278323396656965 2315 24-25 -5.029160393933374 2315 26-27 -4.790433091437745 2315 28-29 -2.6562627870053745 2315 30-31 -3.288332750278446 2315 32-33 -4.343313709653906 2315 34-35 -3.843152052278036 2315 36-37 -5.2042446319689795 2315 38-39 -3.6651047996838813 2315 40-41 -4.472768476064985 2315 42-43 -3.3370120013343367 2315 44-45 -1.9449611023428872 2315 46-47 -1.4833249032560474 2315 48-49 -1.2559745711678474 2315 50-51 -0.9388525851562584 2315 52-53 -0.7444066526468838 2315 54-55 -2.333110085802602 2315 56-57 -4.607406840919644 2315 58-59 -3.8652744712866642 2315 60-61 -4.719733075076679 2315 62-63 -2.523273696962697 2315 64-65 -3.1165178148365875 2315 66-67 -1.8319307098134807 2315 68-69 -1.2613018291759843 2315 70-71 -1.8635801666144651 2315 72-73 -1.9678864637051277 2315 74-75 -2.6859340471088196 2315 76-77 -2.4066557907598956 2315 78-79 -1.547400091681972 2315 80-81 -1.560495960335917 2315 82-83 -1.0698101418005095 2315 84-85 -0.7112844526007187 2315 86-87 -1.322727012429283 2315 88-89 -1.8167995296346646 2315 90-91 -1.3410009167893548 2315 92-93 -0.18295509464055826 2315 94-95 -0.1832080220217307 2315 96-97 -1.0946782761609377 2315 98-99 -0.6474563776982372 2315 100-101 -0.24377378769976943 2316 1 -14.220445384206922 2316 2 -7.427163971505305 2316 3 -5.112193272227135 2316 4 -3.0252246245364347 2316 5 -2.030254591423528 2316 6 -1.1497292419669307 2316 7 -4.785488951233322 2316 8 -3.0155833016540043 2316 9 -1.3797442668800741 2316 10-11 -4.473507021479964 2316 12-13 -3.685867995222342 2316 14-15 -1.9589935602192625 2316 16-17 -2.996740721970255 2316 18-19 -2.3492114463823626 2316 20-21 -3.559574729950512 2316 22-23 -3.2610141931235503 2316 24-25 -3.3658739440223613 2316 26-27 -4.081503741630307 2316 28-29 -3.8171726753436097 2316 30-31 -2.8642625231501526 2316 32-33 -2.6584732387466943 2316 34-35 -4.035170697379456 2316 36-37 -2.184804521217991 2316 38-39 -2.876554960840835 2316 40-41 -2.6788116758264877 2316 42-43 -6.480569071266679 2316 44-45 -3.0853513476970704 2316 46-47 -4.330826335491487 2316 48-49 -4.017723840554595 2316 50-51 -2.9059370823011506 2316 52-53 -4.39430538917534 2316 54-55 -4.476081048816418 2316 56-57 -4.279089282059452 2316 58-59 -3.3597360238786287 2316 60-61 -2.955374994921268 2316 62-63 -1.9999669994885387 2316 64-65 -2.142912317002324 2316 66-67 -2.359107008568177 2316 68-69 -2.691051033396981 2316 70-71 -2.656004224783363 2316 72-73 -3.473793905762129 2316 74-75 -4.299787913682717 2316 76-77 -3.902356903683163 2316 78-79 -4.095858844712243 2316 80-81 -4.028238913776011 2316 82-83 -3.717517679964164 2316 84-85 -3.244765024612338 2316 86-87 -3.3133984326039325 2316 88-89 -2.849168371375022 2316 90-91 -2.239998428945718 2316 92-93 -2.090216085780842 2316 94-95 -1.325746748821505 2316 96-97 -1.6482907677682839 2316 98-99 -2.024964649771917 2316 100-101 -1.7199417234309458 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17813.0 3 5158.0 4 5801.0 5 6880.0 6 7701.0 7 9434.0 8 11761.0 9 13840.0 10 16687.0 11 19605.0 12 22804.0 13 25441.0 14 28149.0 15 30660.0 16 34654.0 17 36858.0 18 35142.0 19 36031.0 20 38949.0 21 42868.0 22 48166.0 23 53525.0 24 59822.0 25 67829.0 26 75867.0 27 87151.0 28 98744.0 29 112281.0 30 128740.0 31 149125.0 32 172172.0 33 201178.0 34 234924.0 35 276197.0 36 296793.0 37 180383.0 38 23583.0 39 139.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.970954546792186 19.413499612531105 14.003322096190379 23.612223744486336 2 15.088077732855657 20.49518615134488 41.18741231239972 23.229323803399744 3 18.75232949223896 25.30668111290131 30.24645935631101 25.694530038548713 4 12.375080922044143 17.02684746919423 38.905398483595675 31.692673125165953 5 13.304474422775659 37.636226420664755 35.34493225352295 13.714366903036643 6 30.698159265079187 37.40988180124552 18.38940954515572 13.502549388519574 7 27.12054490195434 32.86001540851958 22.39263226168938 17.626807427836706 8 25.15070370230915 35.738723154807126 20.74450766678553 18.36606547609819 9 26.0959530322453 16.746546939149134 20.838250107132456 36.31924992147311 10-11 24.180478365986996 26.770049737987062 28.789592113076512 20.259879782949433 12-13 25.22030655229981 24.33334083836632 28.643635073799693 21.80271753553418 14-15 22.470743007999715 25.6681010935013 26.771310429752216 25.08984546874677 16-17 21.88821756168826 28.48947319659814 27.399069919205736 22.223239322507858 18-19 21.722908655332446 27.680139089220308 29.27276234033855 21.324189915108697 20-21 22.70079196902278 26.985673369363145 29.101824641391154 21.21171002022292 22-23 22.2274314348923 26.94493464182026 28.87969039180932 21.94794353147812 24-25 22.00176573694583 27.00186732223037 29.234028484513786 21.762338456310015 26-27 21.968287070767627 27.150019474989733 29.157226975695693 21.724466478546947 28-29 21.896169615484062 27.325508809515014 29.144922986000815 21.633398589000112 30-31 22.148824231422186 27.198952023721322 29.02592843594306 21.62629530891343 32-33 21.933841994806016 27.1914901554963 28.994110271222297 21.880557578475386 34-35 22.185584007419237 27.270022719755882 28.965555830711505 21.578837442113375 36-37 22.195368789097273 27.029725328708366 29.08544395593723 21.689461926257138 38-39 21.865574308056352 27.4232613891162 28.992062108755313 21.719102194072136 40-41 22.200510783997306 27.2500973044992 28.955086311121814 21.594305600381684 42-43 22.00146963251802 27.203064073023754 28.833193016660758 21.96227327779747 44-45 22.117974743755553 27.37317404827448 28.716495443106975 21.792355764862993 46-47 22.17560292475139 27.347482031530085 28.475174023520584 22.00174102019794 48-49 22.077107443878337 27.436704278131764 28.46528746468384 22.020900813306067 50-51 22.076309203991634 27.633735623467814 28.318902845759514 21.97105232678104 52-53 22.187463795444053 27.73778505631142 28.093682192966423 21.9810689552781 54-55 22.166272958751012 27.572800861390796 28.23375349782971 22.027172682028482 56-57 22.131211735113244 27.760179064800905 28.106195416026804 22.00241378405905 58-59 22.106083500239283 27.65099818619023 28.25184113507861 21.99107717849188 60-61 22.174564688777473 27.669583544062355 28.22893637654659 21.926915390613587 62-63 22.033366564854738 27.58564656790522 28.33044750284413 22.050539364395913 64-65 22.018039149507516 27.623243919204725 28.286819929214868 22.07189700207289 66-67 21.974212852359294 27.81818188532833 27.95059648428253 22.25700877802985 68-69 22.052976195311732 27.621226074748478 28.132447504211406 22.193350225728384 70-71 22.112278684156443 27.39259685196603 28.16679952588003 22.328324937997497 72-73 22.04798169058462 27.49640685849542 28.090572653118063 22.365038797801894 74-75 21.899331751776202 27.835362378388005 27.834623390553777 22.430682479282012 76-77 21.935364202880532 27.56961665645326 27.93753940874823 22.55747973191798 78-79 21.968056855140027 27.672118609546736 27.8734466950733 22.48637784023994 80-81 22.055949345152747 27.597140771605915 27.73669913975956 22.61021074348178 82-83 22.027370750210086 27.58943394386083 27.912010000559732 22.471185305369353 84-85 22.119612269561596 27.559218127181023 27.720624735913674 22.600544867343714 86-87 21.87376195792804 27.494438334076737 27.977326687962144 22.654473020033077 88-89 22.06038397284171 27.381942065149232 27.826367218704203 22.731306743304856 90-91 21.897988829993327 27.491727391558562 27.895856179423355 22.71442759902476 92-93 21.872844513561592 27.418369218171655 27.910044042791814 22.79874222547494 94-95 21.8776458808087 27.382020157420385 27.802867042497592 22.937466919273326 96-97 21.846384188475174 27.262654157636256 27.83628244665281 23.05467920723576 98-99 21.79306737541135 27.344083408110702 27.572805126323697 23.290044090154247 100-101 21.949427217972104 27.267965661355642 27.633556268258967 23.149050852413282 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3000.0 1 1958.0 2 835.0 3 686.0 4 594.5 5 554.0 6 715.5 7 1613.0 8 2293.0 9 2117.5 10 1853.5 11 1768.0 12 1901.0 13 2066.0 14 2251.5 15 2459.0 16 2681.0 17 2985.5 18 3379.0 19 3815.5 20 4429.0 21 5406.5 22 6682.0 23 8284.5 24 10278.5 25 13427.0 26 18054.5 27 23047.0 28 27157.0 29 31640.5 30 36906.0 31 42517.5 32 48630.0 33 55437.0 34 62587.5 35 68881.5 36 75083.5 37 79839.5 38 82733.5 39 85899.5 40 89327.0 41 91733.0 42 93204.5 43 95418.0 44 97827.5 45 99196.0 46 100031.5 47 101889.5 48 103335.0 49 101531.0 50 98510.0 51 95369.0 52 91694.0 53 87398.5 54 81960.5 55 75086.5 56 67962.0 57 61794.5 58 55194.0 59 47686.5 60 39152.5 61 30719.0 62 23564.0 63 17371.5 64 12551.5 65 8902.5 66 6183.5 67 4282.0 68 2982.5 69 2074.0 70 1379.0 71 901.0 72 558.0 73 359.5 74 245.0 75 156.0 76 104.5 77 73.0 78 49.0 79 30.5 80 21.5 81 14.0 82 11.0 83 9.0 84 7.0 85 6.0 86 5.5 87 5.0 88 4.0 89 3.5 90 3.5 91 5.0 92 5.5 93 3.0 94 3.0 95 4.0 96 3.5 97 2.5 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 54.90639934681359 2 0.08249611571573122 3 0.11194848231844312 4 0.18438139893212133 5 0.11500798973774862 6 0.13911543374046897 7 0.1461191254232165 8 0.1436494025666687 9 0.13240663433910033 10-11 0.12002115852118893 12-13 0.13130078828392966 14-15 0.13856251071288364 16-17 0.1337336496053051 18-19 0.14016598749288112 20-21 0.16742509275283787 22-23 0.1859848757121188 24-25 0.21269105794449022 26-27 0.20408388948174525 28-29 0.2047658278824338 30-31 0.21834930359344676 32-33 0.20953939668725383 34-35 0.195347705645897 36-37 0.18229872219488324 38-39 0.17807807641764856 40-41 0.17918392247281922 42-43 0.17306490763420826 44-45 0.18758835249211625 46-47 0.21458942700586653 48-49 0.21670896527827693 50-51 0.22645884133136496 52-53 0.25347834661270136 54-55 0.2408532708161697 56-57 0.21755678058724112 58-59 0.17483426132248128 60-61 0.1840680758831563 62-63 0.18742247558384065 64-65 0.17695379959489174 66-67 0.18696170639418622 68-69 0.17446564597075773 70-71 0.18804912168177068 72-73 0.2069406584576028 74-75 0.237775332629278 76-77 0.2375541634182439 78-79 0.2332413638030783 80-81 0.23814394798100155 82-83 0.2287811180472233 84-85 0.19931032067692522 86-87 0.21038721199621802 88-89 0.21241459643069754 90-91 0.20001068984519996 92-93 0.19811232078382365 94-95 0.23619028661686675 96-97 0.2647764071430283 98-99 0.28023982114783136 100-101 0.2767011137712852 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2712855.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.33415860101215 #Duplication Level Percentage of deduplicated Percentage of total 1 85.07074019800561 64.93803374580061 2 9.686092618757215 14.78759460368613 3 2.6733921107115437 6.12213412145249 4 1.0484871477675703 3.2014153691525027 5 0.5234708017365544 1.9979351601378563 6 0.2791578001757471 1.2785565475995082 7 0.1772628351672438 0.9471846561605024 8 0.11765226728300467 0.7184709464439641 9 0.07632228229260193 0.5243397481183228 >10 0.3317995270868474 4.098070852920487 >50 0.011525885228399948 0.5954015223285594 >100 0.0038900760539774108 0.4822588439920666 >500 5.1612433430232995E-5 0.02174765006944668 >1k 1.548373002753894E-4 0.2868562321376538 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3306 0.1218642352798067 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.686153517235532E-5 0.0 10-11 0.0 0.0 0.0 3.686153517235532E-5 0.0 12-13 0.0 0.0 0.0 1.1058460551706597E-4 0.0 14-15 0.0 0.0 0.0 1.2901537310324362E-4 0.0 16-17 0.0 0.0 0.0 2.764615137926649E-4 1.843076758617766E-5 18-19 0.0 0.0 0.0 3.8704611930973087E-4 3.686153517235532E-5 20-21 0.0 7.372307034471065E-5 0.0 4.6076918965444154E-4 3.686153517235532E-5 22-23 0.0 7.372307034471065E-5 0.0 5.897845627576852E-4 3.686153517235532E-5 24-25 0.0 9.215383793088831E-5 0.0 7.556614710332841E-4 3.686153517235532E-5 26-27 0.0 1.1058460551706597E-4 0.0 0.0010689845199983044 3.686153517235532E-5 28-29 0.0 1.1058460551706597E-4 0.0 0.0021379690399966084 3.686153517235532E-5 30-31 0.0 1.474461406894213E-4 0.0 0.005731968719301253 3.686153517235532E-5 32-33 0.0 1.474461406894213E-4 0.0 0.01347289110549587 3.686153517235532E-5 34-35 0.0 1.474461406894213E-4 0.0 0.026024243831682857 3.686153517235532E-5 36-37 0.0 1.474461406894213E-4 0.0 0.04390208839027519 3.686153517235532E-5 38-39 0.0 1.474461406894213E-4 0.0 0.07313328578195297 3.686153517235532E-5 40-41 0.0 1.474461406894213E-4 0.0 0.10538712905776387 3.686153517235532E-5 42-43 0.0 1.6587690827559896E-4 0.0 0.14055303361219085 3.686153517235532E-5 44-45 0.0 1.8430767586177663E-4 0.0 0.18395749127763925 3.686153517235532E-5 46-47 0.0 1.8430767586177663E-4 0.0 0.235803240497557 3.686153517235532E-5 48-49 0.0 1.8430767586177663E-4 0.0 0.2914457278402274 3.686153517235532E-5 50-51 0.0 1.8430767586177663E-4 0.0 0.349299907293239 3.686153517235532E-5 52-53 0.0 2.027384434479543E-4 0.0 0.40955008653245384 3.686153517235532E-5 54-55 0.0 2.2116921103413194E-4 0.0 0.481043033999237 3.686153517235532E-5 56-57 0.0 2.2116921103413194E-4 0.0 0.563631303552899 3.686153517235532E-5 58-59 0.0 2.2116921103413194E-4 0.0 0.6548267415693061 3.686153517235532E-5 60-61 0.0 2.2116921103413194E-4 0.0 0.7561038094553524 3.686153517235532E-5 62-63 0.0 2.2116921103413194E-4 0.0 0.8627995230117349 3.686153517235532E-5 64-65 0.0 2.3959997862030958E-4 0.0 0.9761118821315551 3.686153517235532E-5 66-67 0.0 2.5803074620648725E-4 0.0 1.106933470458244 3.686153517235532E-5 68-69 0.0 2.5803074620648725E-4 0.0 1.2543796111476655 3.686153517235532E-5 70-71 3.686153517235532E-5 2.5803074620648725E-4 0.0 1.4208278732184358 3.686153517235532E-5 72-73 3.686153517235532E-5 2.5803074620648725E-4 0.0 1.5929896732409214 5.5292302758532985E-5 74-75 3.686153517235532E-5 2.5803074620648725E-4 0.0 1.774919780084081 7.372307034471065E-5 76-77 3.686153517235532E-5 2.5803074620648725E-4 0.0 1.96982514730791 7.372307034471065E-5 78-79 3.686153517235532E-5 2.5803074620648725E-4 0.0 2.1932613427551417 7.372307034471065E-5 80-81 3.686153517235532E-5 2.5803074620648725E-4 0.0 2.4417818128871613 7.372307034471065E-5 82-83 3.686153517235532E-5 2.5803074620648725E-4 0.0 2.7028352049777817 7.372307034471065E-5 84-85 3.686153517235532E-5 2.5803074620648725E-4 0.0 2.9684778581973603 7.372307034471065E-5 86-87 3.686153517235532E-5 2.5803074620648725E-4 0.0 3.253380663544495 7.372307034471065E-5 88-89 3.686153517235532E-5 2.5803074620648725E-4 0.0 3.56889697385227 7.372307034471065E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4810 0.0 44.202286 1 GTATCAA 7485 0.0 43.237488 1 TATCAAC 10030 0.0 30.421839 2 GTACATG 6670 0.0 29.520641 1 ATCAACG 10730 0.0 28.349674 3 TCAACGC 10745 0.0 28.306957 4 CAACGCA 10985 0.0 27.708979 5 AACGCAG 11425 0.0 26.683157 6 TACATGG 7695 0.0 25.412397 2 ACGCAGA 11855 0.0 25.197823 7 ACATGGG 7845 0.0 23.602337 3 CGCAGAG 12495 0.0 23.340227 8 GCAGAGT 13010 0.0 20.421703 9 GTGTAGG 1140 0.0 20.212107 1 GAGTACT 6645 0.0 19.956678 12-13 AGAGTAC 10035 0.0 19.727472 10-11 GAGTACA 5445 0.0 18.85046 1 CATGGGA 4135 0.0 18.73191 4 GTACTTT 7410 0.0 17.866343 14-15 AGTACTT 7385 0.0 17.861101 12-13 >>END_MODULE