FastQCFastQC Report
Fri 27 May 2016
SRR939568_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939568_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2712855
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83990.30960003391261237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65710.24221714761754684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49040.1807689684852305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55800.037.439161
GTATCAA90500.034.964761
TCAACGC109350.028.496734
ATCAACG110750.028.2216383
TATCAAC112350.027.9077632
CAACGCA113300.027.5448535
AACGCAG115700.027.0007846
ACGCAGA130600.023.487357
CGCAGAG128700.023.4313668
GTACATG77050.022.4008771
TACATGG75450.021.8093472
GCAGAGT136050.020.8498139
ACATGGG76750.020.7611183
AGAGTAC106500.020.52922610-11
AGTACTT79650.018.57582312-13
GAGTACT74000.018.43406312-13
CAGAGTA129450.017.0898310-11
CATGGGG42150.016.7766824
TAGCCCT21700.016.7279764
GTACTTT82000.016.60688614-15
Produced by FastQC (version 0.11.5)