FastQCFastQC Report
Fri 27 May 2016
SRR939567_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939567_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567020
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA176650.6881520206309262No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA137920.5372766865860025No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105990.41289121237855564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75770.2951671588067097No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT66490.25901629126380005No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA56400.2197100139461321No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG53840.20973736083084665No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT52770.20556910347406723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52430.20424461048219336No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG45080.17561218845197935No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC40770.15882229199616676No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG33390.130073002937258No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT26930.10490763609165492No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG25860.10073937873487547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG157700.023.440831
TACATGG159500.022.9680332
CATGGGG46050.022.877894
GAGTACT83400.022.2757812-13
ACATGGG161350.021.7635633
TACCTGG18950.021.5355742
AGTACTT88200.020.84828612-13
GTATAGG6500.020.4414581
GTACTTT93050.020.118814-15
CATGGGA114850.019.1725374
ATGGGGA26000.018.0698455
GTGTACG1851.5337458E-417.9553361
CTCGCGC3200.017.80109460-61
TCGCGCT3350.017.71253260-61
ATGGGAT37450.017.6139035
ACTTTTT113200.017.27253716-17
GTACCTG24800.016.8382991
CGGTATA1701.7667844E-816.75446134-35
TACTTTT111900.016.43285614-15
GCTTATA31450.016.2955971
Produced by FastQC (version 0.11.5)