FastQCFastQC Report
Fri 27 May 2016
SRR939567_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939567_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567020
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA167210.6513778622683111No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA146030.5688697400098168No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107280.41791649461242997No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT93640.3647809522325498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84880.33065577985368244No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA67630.2634572383542006No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG65750.25613357122266284No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT61970.2414083256071242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60290.23486377200021813No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC53160.20708837484709897No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG43680.17015839377955763No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG36320.141487016073112No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG30800.1199834827932778No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT29700.1156983584078036No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG28620.11149114537479256No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA26890.10475181338672858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG144950.023.7682671
TACATGG144750.023.244722
ACATGGG146150.021.7646083
TACCTGG17100.020.914832
GAGTACT83850.020.34493612-13
CATGGGG43750.019.7898624
CATGGGA102350.019.3092064
AGTACTT87450.018.94314412-13
GTACTTT92400.018.71630714-15
GCTTATA30900.018.4302621
ATGGGAT36700.018.0782345
GTACCTG22400.017.0192971
ATGGGAG36650.016.4338385
ACTTTTT110600.016.0613116-17
CGACGCA2452.0008883E-1115.8828968-69
ATAGGAG16150.015.7334595
TACTTTT105950.015.50209914-15
GGTATCG3650.015.30807562-63
TTATAGG20250.015.1039763
CTTATAG18300.014.6575242
Produced by FastQC (version 0.11.5)