##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939567_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2567020 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.556293289495212 31.0 31.0 34.0 30.0 34.0 2 31.766588885166456 31.0 31.0 34.0 30.0 34.0 3 31.810831625776192 31.0 31.0 34.0 30.0 34.0 4 35.575300153485365 37.0 35.0 37.0 33.0 37.0 5 35.3166091421181 37.0 35.0 37.0 33.0 37.0 6 35.346441398976246 37.0 35.0 37.0 32.0 37.0 7 35.28328139243169 37.0 35.0 37.0 32.0 37.0 8 35.25376272876721 37.0 35.0 37.0 32.0 37.0 9 36.86035753519646 39.0 37.0 39.0 33.0 39.0 10-11 36.74219600938053 39.0 35.0 39.0 32.0 39.0 12-13 36.6806618179835 39.0 35.0 39.0 32.0 39.0 14-15 37.704384071803105 39.5 37.0 41.0 32.0 41.0 16-17 37.66926416623166 39.0 36.5 41.0 32.0 41.0 18-19 37.685514721350046 39.5 36.5 41.0 32.0 41.0 20-21 37.63958422606758 39.0 36.5 41.0 32.0 41.0 22-23 37.51079870822977 39.0 36.0 41.0 32.0 41.0 24-25 37.37889167205553 39.0 36.0 41.0 31.5 41.0 26-27 37.08315400737041 39.0 36.0 40.5 31.0 41.0 28-29 36.86964320496139 39.0 36.0 40.0 30.5 41.0 30-31 36.65704474448972 38.5 35.5 40.0 30.0 41.0 32-33 36.51458169394863 38.0 35.0 40.0 30.0 41.0 34-35 36.513065344251316 38.0 35.0 40.0 30.0 41.0 36-37 36.40076022002166 38.0 35.0 40.0 30.0 41.0 38-39 36.42119753644303 38.5 35.0 40.0 30.0 41.0 40-41 36.3148797048718 38.5 35.0 40.0 30.0 41.0 42-43 36.163902112176764 38.0 35.0 40.0 30.0 41.0 44-45 35.96645974709975 38.0 35.0 40.0 29.0 41.0 46-47 35.649426572445876 38.0 35.0 40.0 28.0 41.0 48-49 35.34712546065087 38.0 34.0 40.0 27.0 41.0 50-51 35.09687010619317 38.0 34.0 40.0 26.5 41.0 52-53 34.80521071125274 38.0 34.0 40.0 26.0 41.0 54-55 34.52519984261907 38.0 33.0 40.0 25.5 41.0 56-57 32.53797633053112 37.0 30.5 39.5 16.0 41.0 58-59 27.549812038862182 32.5 17.5 38.5 6.5 40.0 60-61 28.285051343581273 33.0 20.0 38.0 9.0 40.0 62-63 31.322181556824646 35.0 29.5 38.5 12.5 40.0 64-65 31.50734450841832 35.5 30.5 39.0 5.0 40.0 66-67 24.81565881839643 27.0 18.0 35.5 2.0 38.5 68-69 17.35304672343807 13.0 7.5 28.0 2.0 35.0 70-71 16.232235042968114 12.5 7.5 26.5 2.0 33.5 72-73 17.328719098409827 13.5 7.0 28.5 2.0 34.0 74-75 18.32405980475415 17.5 8.5 29.5 2.0 34.5 76-77 19.50762304150338 21.5 8.0 30.0 2.0 34.0 78-79 22.58565496178448 27.5 10.5 33.5 2.0 35.5 80-81 26.237975356639218 31.5 22.5 34.5 2.0 36.0 82-83 27.55511371161892 33.0 26.5 35.0 2.0 36.0 84-85 27.62526937850114 34.0 27.0 35.0 2.0 36.0 86-87 27.368019532376064 34.0 26.5 35.0 2.0 36.0 88-89 26.915922158767753 33.0 26.0 35.0 2.0 35.0 90-91 25.576568355525083 32.5 18.5 35.0 2.0 35.0 92-93 23.16014834321509 31.5 2.0 34.5 2.0 35.0 94-95 15.844036080747326 4.5 2.0 32.0 2.0 35.0 96-97 13.266525776970962 2.0 2.0 30.0 2.0 34.0 98-99 12.938278626578679 2.0 2.0 29.5 2.0 34.0 100-101 12.645877905119555 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38010094398726935 1101 2 0.36697454862169465 1101 3 0.3908256062757296 1101 4 0.27346560297385025 1101 5 0.25463214623347596 1101 6 0.3144346548879753 1101 7 0.3882963725921158 1101 8 0.32742075133086956 1101 9 0.3854778804155359 1101 10-11 0.46432772506177855 1101 12-13 0.3850663756406334 1101 14-15 0.5515830826536146 1101 16-17 0.5319314916559748 1101 18-19 0.35408923765109535 1101 20-21 0.41241243621166035 1101 22-23 0.5330116089702273 1101 24-25 0.5590648801526754 1101 26-27 0.37218990200155133 1101 28-29 0.531568660879131 1101 30-31 0.5334093857424662 1101 32-33 0.6416847330676845 1101 34-35 0.43375500466931527 1101 36-37 0.017655120223814436 1101 38-39 0.28329836107840123 1101 40-41 0.1528935076450182 1101 42-43 0.24990869563841756 1101 44-45 0.07924372200022844 1101 46-47 -0.02652521952080633 1101 48-49 -0.026199851749872494 1101 50-51 -0.2041252968561551 1101 52-53 -0.3124621411561179 1101 54-55 -0.2793431321388482 1101 56-57 -2.7491995755214056 1101 58-59 -10.210145673317673 1101 60-61 -10.348453539547886 1101 62-63 -4.406213207817849 1101 64-65 -5.912482677692886 1101 66-67 -10.404232866323586 1101 68-69 -5.251854288831353 1101 70-71 -5.036754262345159 1101 72-73 -5.216229669051341 1101 74-75 -6.6010000915712155 1101 76-77 -8.250814119746916 1101 78-79 -11.514206386803645 1101 80-81 -8.674508741823164 1101 82-83 -4.987182523151056 1101 84-85 -4.2313328670537125 1101 86-87 -3.9855606844361624 1101 88-89 -4.038062728978527 1101 90-91 -10.239742923515001 1101 92-93 -17.256405141459354 1101 94-95 -11.436973449903025 1101 96-97 -9.580709853371225 1101 98-99 -9.30916165595266 1101 100-101 -9.198083904653457 1102 1 0.30720530141997315 1102 2 0.340429742061108 1102 3 0.3395577866917172 1102 4 0.12661868674448584 1102 5 0.21318767110122394 1102 6 0.2555941760165297 1102 7 0.3799495838351845 1102 8 0.33499208278043824 1102 9 0.5056365835959724 1102 10-11 0.4156033788891378 1102 12-13 0.361276832629315 1102 14-15 0.43752845009290553 1102 16-17 0.4495637177495908 1102 18-19 0.45180482540524025 1102 20-21 0.39511602031159754 1102 22-23 0.5902606741723844 1102 24-25 0.5220679979159613 1102 26-27 0.4560123222781627 1102 28-29 0.541216066815096 1102 30-31 0.3947704992141823 1102 32-33 0.509301954593532 1102 34-35 0.34438421391532614 1102 36-37 0.3440761722963046 1102 38-39 0.4143048356157806 1102 40-41 0.2647578716637966 1102 42-43 0.3242961640358786 1102 44-45 0.43039442628543156 1102 46-47 0.37764273072244947 1102 48-49 0.38198480800495105 1102 50-51 0.2145924366839722 1102 52-53 0.285017091459828 1102 54-55 0.22055130475168028 1102 56-57 -3.100767884812022 1102 58-59 -10.684631932851548 1102 60-61 -10.891160124561377 1102 62-63 -4.749491825379302 1102 64-65 -5.808365583639471 1102 66-67 -7.3120072241811975 1102 68-69 -4.739875863540824 1102 70-71 -4.730557508643146 1102 72-73 -5.168372400998058 1102 74-75 -5.937171457013438 1102 76-77 -8.038200058475166 1102 78-79 -10.48975823621621 1102 80-81 -6.5505119233226665 1102 82-83 -4.133817649242538 1102 84-85 -3.658773167463746 1102 86-87 -3.4608336878991643 1102 88-89 -3.538880013178318 1102 90-91 -9.383302777134155 1102 92-93 -14.188491546524256 1102 94-95 -10.72472122444678 1102 96-97 -9.14685344044552 1102 98-99 -9.418331836963517 1102 100-101 -9.330072979203585 1103 1 0.22885146709362303 1103 2 0.2589695268006871 1103 3 0.2908086675937618 1103 4 0.2588973400855039 1103 5 0.25235738088404247 1103 6 0.26624908664501135 1103 7 0.15109603780405223 1103 8 0.19551325646229145 1103 9 0.4004088303766764 1103 10-11 0.3231891467153929 1103 12-13 0.2410272971049352 1103 14-15 0.46306900361971515 1103 16-17 0.36210576080167556 1103 18-19 0.3622875597251891 1103 20-21 0.26969158569054485 1103 22-23 0.36538840496871217 1103 24-25 0.4250634652745333 1103 26-27 0.48343320125824363 1103 28-29 0.4163173705501251 1103 30-31 0.33304769506272436 1103 32-33 0.27095228453138276 1103 34-35 0.36124748190173506 1103 36-37 0.042720383969054865 1103 38-39 0.08148442751789275 1103 40-41 0.1637860765455983 1103 42-43 0.19769172108629363 1103 44-45 0.16375578832015947 1103 46-47 0.23174733383604007 1103 48-49 0.27649638786272845 1103 50-51 0.17335821283875674 1103 52-53 0.1799767301298303 1103 54-55 0.021271642377406863 1103 56-57 -2.8420713795906742 1103 58-59 -10.36865437182906 1103 60-61 -10.197193102330612 1103 62-63 -4.2493440740819715 1103 64-65 -5.327637317895352 1103 66-67 -6.582980300611464 1103 68-69 -4.240755964764476 1103 70-71 -4.152109177552109 1103 72-73 -4.538056157164371 1103 74-75 -5.336868747038423 1103 76-77 -6.859135496363564 1103 78-79 -9.420239048568057 1103 80-81 -5.279772686344 1103 82-83 -2.9848414976633215 1103 84-85 -2.6155056615376786 1103 86-87 -2.4333591539764257 1103 88-89 -2.439645997546311 1103 90-91 -5.556679114503623 1103 92-93 -10.74011560956481 1103 94-95 -8.712550361483105 1103 96-97 -8.14292862143085 1103 98-99 -8.367303183622496 1103 100-101 -8.441361738893509 1104 1 0.25794656089000156 1104 2 0.2874021364815711 1104 3 0.1962335431095461 1104 4 0.13504006223910636 1104 5 0.2746903380247403 1104 6 0.22107042476517336 1104 7 0.24193944984162385 1104 8 0.19002884179729307 1104 9 0.2806818218190372 1104 10-11 0.3372572701658214 1104 12-13 0.3310023558294972 1104 14-15 0.46029065809715775 1104 16-17 0.43429275816239965 1104 18-19 0.4067758192566302 1104 20-21 0.3052869623719303 1104 22-23 0.39110502231564226 1104 24-25 0.4395853155498628 1104 26-27 0.5011204755796896 1104 28-29 0.4813224950639565 1104 30-31 0.4799058941367562 1104 32-33 0.5387067995835295 1104 34-35 0.49147307899647075 1104 36-37 0.29027209564554113 1104 38-39 0.41783004191155726 1104 40-41 0.24548711892503405 1104 42-43 0.3984672110994154 1104 44-45 0.39664990934754485 1104 46-47 0.3675532450960475 1104 48-49 0.31316872704038445 1104 50-51 0.23242008615958554 1104 52-53 0.28749532500590647 1104 54-55 0.2644607043544056 1104 56-57 -2.828772969967254 1104 58-59 -10.03682580903303 1104 60-61 -10.201538053203407 1104 62-63 -4.2916477504022765 1104 64-65 -5.514837215405073 1104 66-67 -6.82248415584543 1104 68-69 -4.45993119944055 1104 70-71 -4.294222609998872 1104 72-73 -4.485539221090461 1104 74-75 -5.264795273951007 1104 76-77 -6.8986378705241655 1104 78-79 -9.276533832523885 1104 80-81 -5.086839183575872 1104 82-83 -2.773515433438053 1104 84-85 -2.2599317159878645 1104 86-87 -2.0604821245356497 1104 88-89 -2.0722715259192306 1104 90-91 -4.875586719222916 1104 92-93 -7.141506559614129 1104 94-95 -7.805958177995889 1104 96-97 -7.776771814269564 1104 98-99 -8.186240706023732 1104 100-101 -8.226005822153528 1105 1 0.33422993526279043 1105 2 0.3491509041913652 1105 3 0.3969024690364016 1105 4 0.24445149889547935 1105 5 0.2836988552487725 1105 6 0.22946180102051983 1105 7 0.2509433934653629 1105 8 0.28636656824583895 1105 9 0.404736746332496 1105 10-11 0.4994978474427043 1105 12-13 0.4501126282832928 1105 14-15 0.579491738647107 1105 16-17 0.5910385972926449 1105 18-19 0.5338336074340262 1105 20-21 0.4539452745953909 1105 22-23 0.6007270658887762 1105 24-25 0.5035413617756817 1105 26-27 0.49950724872763175 1105 28-29 0.578204922553951 1105 30-31 0.6646899685654688 1105 32-33 0.5688092777372162 1105 34-35 0.6649259111615677 1105 36-37 0.5680092110020709 1105 38-39 0.5967945050372663 1105 40-41 0.49170652190790776 1105 42-43 0.4002253178059334 1105 44-45 0.5081086960451557 1105 46-47 0.6169924626858148 1105 48-49 0.4992785554123387 1105 50-51 0.4428734922377515 1105 52-53 0.5026915545888428 1105 54-55 0.43297213024068526 1105 56-57 1.6118420022296789 1105 58-59 -8.59522795537939 1105 60-61 -10.06976126850343 1105 62-63 -3.416761242080536 1105 64-65 -4.543331999287709 1105 66-67 -5.872057999572885 1105 68-69 -3.55357883747107 1105 70-71 -3.6647577064797083 1105 72-73 -2.0909219797976366 1105 74-75 -4.351872787623858 1105 76-77 -6.066641257939093 1105 78-79 -8.608710659714088 1105 80-81 -4.044062890169091 1105 82-83 -1.608269410626118 1105 84-85 -1.1521500301587935 1105 86-87 -1.0253116546498582 1105 88-89 -1.0317967862087265 1105 90-91 -3.514472206599038 1105 92-93 -5.926914119037736 1105 94-95 -7.793198202014667 1105 96-97 -8.22410024155596 1105 98-99 -8.503081071144571 1105 100-101 -8.484971627852234 1106 1 0.2015268051426844 1106 2 0.15848712496669748 1106 3 0.13378431661935153 1106 4 0.027334856686586306 1106 5 0.07043181910351137 1106 6 0.1591644609814793 1106 7 0.2186776538195474 1106 8 0.0881446246364419 1106 9 0.1408435436939115 1106 10-11 0.21025808052765882 1106 12-13 0.2142651308121799 1106 14-15 0.2855347083432136 1106 16-17 0.17339623167979568 1106 18-19 0.17674132801702314 1106 20-21 0.2554718670813898 1106 22-23 0.3262987366633965 1106 24-25 0.37100207499285887 1106 26-27 0.3557575158635089 1106 28-29 0.4019935504016203 1106 30-31 0.3573896793463618 1106 32-33 0.2318597557323372 1106 34-35 0.37935338555213605 1106 36-37 0.332642558107743 1106 38-39 0.40578238063031336 1106 40-41 0.3578856952826541 1106 42-43 0.17605985028253457 1106 44-45 0.2059559370658306 1106 46-47 0.14339332321952014 1106 48-49 0.35296075632701474 1106 50-51 0.30638700575126876 1106 52-53 0.3130899076071145 1106 54-55 0.3064686895306039 1106 56-57 1.3147733732422324 1106 58-59 -9.43147081394697 1106 60-61 -10.251942237031262 1106 62-63 -3.9996104064795155 1106 64-65 -5.713329434783297 1106 66-67 -7.056133851914417 1106 68-69 -4.49055685396938 1106 70-71 -4.328289029051621 1106 72-73 -4.898701688868858 1106 74-75 -5.319436081017411 1106 76-77 -6.754867333250038 1106 78-79 -9.330749013302256 1106 80-81 -5.139422561912522 1106 82-83 -2.6846952038415814 1106 84-85 -2.197488331032151 1106 86-87 -2.1109440186955055 1106 88-89 -2.1356406933736665 1106 90-91 -4.707878580817223 1106 92-93 -6.983528403327298 1106 94-95 -7.824431728635648 1106 96-97 -7.452039804735374 1106 98-99 -6.909632667548565 1106 100-101 -7.381552288639536 1107 1 0.102918990784989 1107 2 -0.007284905706420375 1107 3 -0.05110107204319192 1107 4 0.0031225422430267713 1107 5 -0.04302709236252866 1107 6 -0.07675437137045549 1107 7 0.04224598193026452 1107 8 0.11320280938400629 1107 9 0.08425998612815988 1107 10-11 0.05307150379728398 1107 12-13 0.04611445141337356 1107 14-15 0.03821836701050785 1107 16-17 -0.06262102957924043 1107 18-19 0.09048160454739929 1107 20-21 0.04344014485076286 1107 22-23 0.03904369450052059 1107 24-25 0.19372553005293014 1107 26-27 0.09051993321364904 1107 28-29 0.19526901112768513 1107 30-31 0.0665728470658209 1107 32-33 0.049341447656445325 1107 34-35 -0.06441618429857954 1107 36-37 0.03482700171316111 1107 38-39 -0.05630802996084583 1107 40-41 -0.08240086746622666 1107 42-43 -0.04041244173080116 1107 44-45 0.03123834425993266 1107 46-47 0.08851796094229769 1107 48-49 -0.11412539499502117 1107 50-51 -0.06543073383037523 1107 52-53 -0.10532868390813377 1107 54-55 -0.1727817588843763 1107 56-57 0.9941203158927863 1107 58-59 -9.03670248487889 1107 60-61 -10.472503267840207 1107 62-63 -3.9859675815479427 1107 64-65 -5.364939669873813 1107 66-67 -6.934221622576462 1107 68-69 -4.261009772541051 1107 70-71 -4.388274915922002 1107 72-73 -2.575007208207497 1107 74-75 -4.943460094080631 1107 76-77 -6.9236536619435505 1107 78-79 -7.570065487868007 1107 80-81 -2.7557557570265416 1107 82-83 -1.6779263466281158 1107 84-85 -1.4708314893056347 1107 86-87 -1.3033511908619246 1107 88-89 -1.0628485774194267 1107 90-91 -0.7525776705841878 1107 92-93 -3.371648443113717 1107 94-95 -5.871018319846135 1107 96-97 -6.474021063951241 1107 98-99 -6.118906585324227 1107 100-101 -6.626599180490002 1108 1 -0.13568320280040425 1108 2 -0.1518360019077143 1108 3 -0.12291588344102067 1108 4 -0.10225187973140493 1108 5 -0.06686865125841734 1108 6 -0.22447510230954748 1108 7 -0.12624686357042947 1108 8 -0.17895894746585128 1108 9 -0.12948844717919883 1108 10-11 -0.21890738330162662 1108 12-13 -0.18214997109957665 1108 14-15 -0.2665430288477566 1108 16-17 -0.19907075758316495 1108 18-19 -0.23744423006301218 1108 20-21 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4.969620895662413 2316 94-95 11.846814904145228 2316 96-97 13.59802543589082 2316 98-99 13.237783581187971 2316 100-101 11.97175041529183 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 20.0 4 33.0 5 95.0 6 230.0 7 551.0 8 1224.0 9 2156.0 10 3459.0 11 5516.0 12 8743.0 13 12281.0 14 15264.0 15 21014.0 16 29095.0 17 39653.0 18 43372.0 19 43496.0 20 44278.0 21 44733.0 22 44771.0 23 38068.0 24 37967.0 25 44510.0 26 62515.0 27 93865.0 28 133257.0 29 180498.0 30 225901.0 31 247671.0 32 249058.0 33 227333.0 34 203189.0 35 182943.0 36 148327.0 37 94461.0 38 35179.0 39 2291.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.30405257444422 19.541711443732083 14.362157684367864 22.79207829745583 2 15.111435941329532 21.74558235881021 39.485299073441716 23.65768262641854 3 19.47465031355497 26.304803947309985 30.606533505465883 23.614012233669158 4 12.681708751782223 17.17197372829195 37.88575858388326 32.26055893604257 5 13.054660698583998 38.77118561418483 34.29418736107394 13.879966326157225 6 30.252043225218344 36.994452711704625 18.07940725043046 14.67409681264657 7 27.321524569345 33.97628378431021 21.147439443401296 17.55475220294349 8 25.36260451954512 34.837614881900315 20.37940244271098 19.42037815584359 9 26.507740492867214 17.066520712733052 20.600579660462326 35.825159133937404 10-11 24.356140583244386 26.561674626609843 27.956813737329668 21.125371052816106 12-13 26.54948149994936 24.101292549337362 27.936576263527357 21.41264968718592 14-15 23.373614410732202 25.07441068935044 26.99429509932495 24.557679800592403 16-17 21.631502676254957 28.679597354130472 27.791739059298333 21.897160910316245 18-19 21.50440705908568 27.96326321046444 29.64714310534636 20.885186625103525 20-21 22.40025399100903 26.900647443338972 28.888321867379297 21.810776698272704 22-23 22.06417623308367 26.7485959829911 28.387370596018442 22.799857187906785 24-25 22.272643872133397 26.88404332028707 28.92705507828341 21.91625772929612 26-27 22.669099326376347 26.452914906513875 29.098481035538594 21.77950473157118 28-29 21.901155408107396 26.564413461457924 29.756477439866362 21.777953690568317 30-31 21.831705455033525 27.32844648417053 29.27305634954355 21.566791711252396 32-33 21.611405462250758 27.31916201571089 28.796910569390743 22.27252195264761 34-35 22.25783598566593 26.59691693240638 28.72487987902878 22.420367202898912 36-37 22.705394077599763 26.503857369462192 28.969687988386454 21.82106056455159 38-39 22.955305996781135 26.1249936141396 29.414637912940933 21.50506247613833 40-41 22.150670786583685 26.395868902394433 29.870600933904196 21.58285937711769 42-43 21.70298928867153 27.249669549100773 29.05793509238389 21.989406069843806 44-45 22.084340873634943 27.4429992199688 28.253705148205928 22.218954758190325 46-47 21.956999898603062 27.145929692923275 27.64088324532599 23.256187163147672 48-49 22.49012108194749 27.02558037729521 27.903738925520805 22.580559615236496 50-51 22.826444511564848 27.275292297114767 27.958932452803776 21.93933073851661 52-53 22.377136092930385 27.300601596602565 28.331809798076506 21.990452512390544 54-55 21.4892517972149 27.045920595069163 28.78652907511657 22.67829853259937 56-57 21.383267309300155 27.99304108583503 28.540615894847775 22.08307571001704 58-59 20.867323953303906 26.752552771321668 29.90380500386509 22.47631827150933 60-61 22.022255979197105 26.617487814108404 28.87131733712156 22.48893886957293 62-63 23.222600370252632 26.602110097175736 28.007270259488504 22.168019273083132 64-65 22.305568084735867 26.72063672026816 28.8562330673247 22.117562127671274 66-67 21.459359440890942 26.905515357692384 29.556622278405932 22.078502923010742 68-69 21.75867159440906 26.776336732983776 29.59155280817969 21.873438864427477 70-71 21.556503898630012 26.844028362703575 29.189294849866894 22.410172888799522 72-73 20.843967520881403 26.615106605855317 29.49630648218476 23.044619391078523 74-75 21.603325939618482 26.808363416253954 28.99100632287814 22.597304321249425 76-77 20.20265667137632 26.907859143361847 29.948045006460056 22.94143917880178 78-79 20.586468823152064 26.96562856882789 29.548228780089726 22.899673827930325 80-81 21.329064155569046 27.973663876305576 28.145821133014575 22.551450835110803 82-83 21.730545759250738 27.794504970956496 27.406466119545257 23.068483150247506 84-85 21.862078247738083 26.99372247165821 27.34167545281706 23.802523827786647 86-87 22.23336142698342 26.96269725530817 27.865303965567715 22.93863735214069 88-89 22.59667103944558 26.361117522572403 28.00576934126861 23.0364420967134 90-91 21.283957868411715 26.926691481578313 28.92007881464831 22.86927183536166 92-93 20.17088445100496 27.63196410550519 29.03633660592022 23.160814837569628 94-95 19.402875930870195 27.540245306999385 29.253646578920385 23.80323218321004 96-97 19.428770871960165 27.18598691821279 28.710745309053582 24.674496900773466 98-99 19.83294732319666 27.04106193117003 28.467755396322946 24.658235349310367 100-101 20.644942481503836 27.13766099968739 28.356353183817358 23.861043334991418 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 610.0 1 470.0 2 348.0 3 395.5 4 430.5 5 491.0 6 661.5 7 939.5 8 1284.5 9 1667.0 10 1990.5 11 2251.0 12 2539.0 13 2918.0 14 3304.0 15 3584.5 16 3825.0 17 4164.5 18 4557.0 19 4984.0 20 5491.5 21 6189.0 22 7077.5 23 8333.0 24 9868.0 25 11801.5 26 14584.5 27 17856.0 28 21403.5 29 25317.5 30 29460.5 31 34052.0 32 39359.0 33 45134.0 34 51333.0 35 57307.0 36 62998.5 37 68145.0 38 71775.0 39 74905.5 40 77906.5 41 81452.5 42 85385.5 43 89351.0 44 94510.5 45 100519.5 46 107704.5 47 116574.0 48 127224.5 49 125441.0 50 112220.0 51 101818.0 52 92854.0 53 85008.0 54 77350.5 55 69185.5 56 61553.5 57 53913.0 58 45961.5 59 38366.0 60 30977.5 61 24324.5 62 18716.0 63 13989.5 64 10191.0 65 7320.0 66 5060.5 67 3369.0 68 2179.0 69 1325.0 70 740.5 71 440.5 72 264.0 73 142.0 74 83.0 75 52.0 76 26.0 77 9.5 78 3.0 79 2.5 80 1.5 81 0.5 82 0.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02345131709141339 2 0.008141736332400994 3 2.3373405738950223E-4 4 0.0 5 0.14047416849109082 6 0.0 7 0.0 8 0.0014024043443370134 9 0.0 10-11 0.0 12-13 0.0 14-15 6.427686578211312E-4 16-17 0.0 18-19 7.791135246316741E-5 20-21 0.0 22-23 4.090346004316289E-4 24-25 3.8955676231583705E-5 26-27 3.1164540985266964E-4 28-29 0.003564444375189909 30-31 0.013069629375696333 32-33 0.002902197879252986 34-35 0.005005804395758506 36-37 0.10800461235206582 38-39 0.10755662207540259 40-41 0.11891220169690925 42-43 0.12072364064167788 44-45 0.11764614221938278 46-47 0.11063412049769773 48-49 0.1109262880694346 50-51 0.10929014966770809 52-53 0.08809826179772655 54-55 0.07557401188927239 56-57 0.5467623937483931 58-59 1.5548573832693162 60-61 0.512052886226052 62-63 0.004830503852716379 64-65 0.11708128491402482 66-67 1.8815007284711454 68-69 2.8376093680610204 70-71 2.7464530856791143 72-73 2.6033688868805074 74-75 1.9835840780360108 76-77 1.3905033852482647 78-79 0.9244376748135971 80-81 0.05229799534090113 82-83 3.5060108608425336E-4 84-85 2.532118955052941E-4 86-87 4.090346004316289E-4 88-89 9.738919057895926E-5 90-91 0.24653099703157744 92-93 0.3884075698670053 94-95 1.9799612001464733 96-97 2.394819674174724 98-99 1.3939314847566437 100-101 0.9320145538406401 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2567020.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.74690797859964 #Duplication Level Percentage of deduplicated Percentage of total 1 68.99735924541528 28.804264071847328 2 15.301993172621673 12.776218017331939 3 6.084490983562358 7.620260555621909 4 2.9772925386121534 4.971710305392516 5 1.7550415172964944 3.663377836059932 6 1.0952313954272723 2.7433514568105366 7 0.7058536471787442 2.062704507859098 8 0.5248296780122932 1.7528013017933826 9 0.36405589205961914 1.3678387042391933 >10 1.9394785853536507 14.774503931820357 >50 0.14921696489741282 4.3192447362580095 >100 0.0946396818999567 7.327957125213418 >500 0.006572936039431326 1.9266782866826329 >1k 0.0030047707608826025 2.3303595277050073 >5k 6.572936039430693E-4 1.9102927012535396 >10k+ 2.81697258832744E-4 1.6484369341113403 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 16721 0.6513778622683111 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 14603 0.5688697400098168 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10728 0.41791649461242997 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 9364 0.3647809522325498 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8488 0.33065577985368244 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6763 0.2634572383542006 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 6575 0.25613357122266284 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6197 0.2414083256071242 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6029 0.23486377200021813 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5316 0.20708837484709897 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4368 0.17015839377955763 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3632 0.141487016073112 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3080 0.1199834827932778 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2970 0.1156983584078036 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2862 0.11149114537479256 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2689 0.10475181338672858 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.8955676231583705E-5 0.0 6 0.0 0.0 0.0 3.8955676231583705E-5 0.0 7 0.0 0.0 0.0 3.8955676231583705E-5 0.0 8 0.0 0.0 0.0 3.8955676231583705E-5 0.0 9 0.0 0.0 0.0 3.8955676231583705E-5 0.0 10-11 0.0 0.0 0.0 5.843351434737556E-5 0.0 12-13 0.0 0.0 0.0 7.791135246316741E-5 0.0 14-15 0.0 0.0 0.0 7.791135246316741E-5 0.0 16-17 0.0 0.0 0.0 1.5582270492633482E-4 0.0 18-19 0.0 0.0 0.0 2.9216757173687775E-4 0.0 20-21 0.0 0.0 0.0 3.1164540985266964E-4 0.0 22-23 0.0 0.0 0.0 4.4799027666321263E-4 0.0 24-25 0.0 0.0 0.0 0.00105180325825276 0.0 26-27 0.0 0.0 0.0 0.0016750940779580994 0.0 28-29 0.0 0.0 0.0 0.003544966537074117 0.0 30-31 0.0 0.0 0.0 0.009641529867316968 0.0 32-33 0.0 0.0 0.0 0.02243846950939221 0.0 34-35 0.0 0.0 0.0 0.043143411426478956 0.0 36-37 0.0 0.0 0.0 0.07611939135651455 0.0 38-39 0.0 0.0 0.0 0.11963288170719355 0.0 40-41 0.0 0.0 0.0 0.1708790737898419 0.0 42-43 0.0 0.0 0.0 0.21951523556497415 0.0 44-45 0.0 0.0 0.0 0.27701381368279177 0.0 46-47 0.0 0.0 0.0 0.344543478430242 0.0 48-49 0.0 0.0 0.0 0.4136313702269558 0.0 50-51 0.0 0.0 0.0 0.4770901668082056 0.0 52-53 1.9477838115791853E-5 0.0 0.0 0.5399451504078658 0.0 54-55 3.8955676231583705E-5 0.0 0.0 0.6046505286285264 0.0 56-57 3.8955676231583705E-5 0.0 0.0 0.6578639823608698 1.9477838115791853E-5 58-59 3.8955676231583705E-5 0.0 0.0 0.6994491667380854 3.8955676231583705E-5 60-61 3.8955676231583705E-5 0.0 0.0 0.7374893845782269 3.8955676231583705E-5 62-63 7.791135246316741E-5 1.9477838115791853E-5 0.0 0.7718093353382521 3.8955676231583705E-5 64-65 7.791135246316741E-5 3.8955676231583705E-5 0.0 0.8014546049504874 3.8955676231583705E-5 66-67 7.791135246316741E-5 3.8955676231583705E-5 0.0 0.8271458734252168 3.8955676231583705E-5 68-69 7.791135246316741E-5 3.8955676231583705E-5 0.0 0.8627318836627684 7.791135246316741E-5 70-71 7.791135246316741E-5 3.8955676231583705E-5 0.0 0.9210680088195651 7.791135246316741E-5 72-73 7.791135246316741E-5 3.8955676231583705E-5 0.0 1.0092247041316391 7.791135246316741E-5 74-75 7.791135246316741E-5 3.8955676231583705E-5 0.0 1.1185538094755787 7.791135246316741E-5 76-77 7.791135246316741E-5 3.8955676231583705E-5 0.0 1.2816807036953355 7.791135246316741E-5 78-79 7.791135246316741E-5 3.8955676231583705E-5 0.0 1.5319319678070291 7.791135246316741E-5 80-81 7.791135246316741E-5 3.8955676231583705E-5 0.0 1.8411426478952249 7.791135246316741E-5 82-83 7.791135246316741E-5 3.8955676231583705E-5 0.0 2.145503346292588 7.791135246316741E-5 84-85 7.791135246316741E-5 3.8955676231583705E-5 0.0 2.4059025640626093 7.791135246316741E-5 86-87 7.791135246316741E-5 3.8955676231583705E-5 0.0 2.6114716675366765 7.791135246316741E-5 88-89 7.791135246316741E-5 3.8955676231583705E-5 0.0 2.792070182546299 7.791135246316741E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14495 0.0 23.768267 1 TACATGG 14475 0.0 23.24472 2 ACATGGG 14615 0.0 21.764608 3 TACCTGG 1710 0.0 20.91483 2 GAGTACT 8385 0.0 20.344936 12-13 CATGGGG 4375 0.0 19.789862 4 CATGGGA 10235 0.0 19.309206 4 AGTACTT 8745 0.0 18.943144 12-13 GTACTTT 9240 0.0 18.716307 14-15 GCTTATA 3090 0.0 18.430262 1 ATGGGAT 3670 0.0 18.078234 5 GTACCTG 2240 0.0 17.019297 1 ATGGGAG 3665 0.0 16.433838 5 ACTTTTT 11060 0.0 16.06131 16-17 CGACGCA 245 2.0008883E-11 15.88289 68-69 ATAGGAG 1615 0.0 15.733459 5 TACTTTT 10595 0.0 15.502099 14-15 GGTATCG 365 0.0 15.308075 62-63 TTATAGG 2025 0.0 15.103976 3 CTTATAG 1830 0.0 14.657524 2 >>END_MODULE