FastQCFastQC Report
Fri 27 May 2016
SRR939566_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939566_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555588
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA77190.30204399144149996No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA59640.23337095024706642No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44320.17342388522719626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31850.12462885253804604No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT26190.1024813076286162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG136900.026.328751
GAGTACT83900.022.22205712-13
TACATGG157400.022.001962
CATGGGG42850.021.9405424
ACATGGG154100.021.493723
AGTACTT88100.021.48412112-13
GTACTTT93750.019.76526614-15
GCTTATA26150.019.3047351
TACCTGG17800.019.1106132
CATGGGA113050.018.9727554
ATGGGAT39250.018.8804975
TGTACCG2403.285022E-617.7004645
ATGGGAG34100.017.5793485
ATGGGGA26750.017.1159635
GTATATG10151.8189894E-1216.9101851
ACTTTTT116800.016.61536416-17
GTGTAAG10950.016.5967831
TACTTTT115250.016.18044514-15
GTACCGT2355.4233133E-516.0685086
GTATAGG5551.2615888E-414.5533351
Produced by FastQC (version 0.11.5)