FastQCFastQC Report
Fri 27 May 2016
SRR939566_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939566_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555588
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA164680.6443918190256019No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA144160.5640971862444181No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106770.41779034805297255No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT91350.35745198365307707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83110.3252089147389955No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG65470.2561837041025392No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA65110.25477502633444826No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT60330.2360709159692407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59570.23309704068104875No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC50990.19952355387488122No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG42550.16649788620074912No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG36240.14180689532115504No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG29730.11633330568151048No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT29600.11582461648747763No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA27670.10827253845299009No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG26790.10482910390876776No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25810.10099436998452019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG148900.023.8054051
TACATGG149300.023.261962
ACATGGG148600.022.5110723
AGTACTT83050.020.83805712-13
CATGGGG41550.020.7801574
GAGTACT80850.020.44272412-13
ATGGGAG35350.020.02035
CATGGGA109200.019.5292054
TACCTGG19950.019.3954222
GTACTTT91000.018.52530914-15
ATGGGAT39300.017.8880275
GCTTATA28150.017.7728791
GTACCTG24300.016.7040771
ATGGGGA25450.016.4995085
TACTTTT104500.016.42539814-15
ACTTTTT106100.016.2888116-17
TAGGACA10300.016.0324364
GCGTTTA10300.015.75760462-63
TACGCGG1551.6319482E-615.67368764-65
CGTTGAG8650.015.40278362-63
Produced by FastQC (version 0.11.5)