##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939566_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2555588 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.486418389818702 31.0 31.0 34.0 30.0 34.0 2 31.692306819409076 31.0 31.0 34.0 30.0 34.0 3 31.744438461911702 31.0 31.0 34.0 30.0 34.0 4 35.5244558199522 37.0 35.0 37.0 33.0 37.0 5 35.28663383925735 37.0 35.0 37.0 33.0 37.0 6 35.297814045143426 37.0 35.0 37.0 32.0 37.0 7 35.21279838534224 37.0 35.0 37.0 32.0 37.0 8 35.17702383952343 37.0 35.0 37.0 32.0 37.0 9 36.76906997528553 39.0 35.0 39.0 32.0 39.0 10-11 36.65025133159179 39.0 35.0 39.0 32.0 39.0 12-13 36.585233613555864 39.0 35.0 39.0 32.0 39.0 14-15 37.617180664488956 39.0 36.0 41.0 32.0 41.0 16-17 37.57279224976796 39.0 36.0 41.0 32.0 41.0 18-19 37.580626845954825 39.0 36.0 41.0 32.0 41.0 20-21 37.52906043540665 39.0 36.0 41.0 32.0 41.0 22-23 37.40387398125206 39.0 36.0 41.0 32.0 41.0 24-25 37.265130568777124 39.0 36.0 41.0 31.0 41.0 26-27 36.94270085788476 39.0 36.0 40.0 30.5 41.0 28-29 36.73261319899765 39.0 35.5 40.0 30.0 41.0 30-31 36.51620018563243 38.0 35.0 40.0 30.0 41.0 32-33 36.36993032523239 38.0 35.0 40.0 30.0 41.0 34-35 36.35932200339022 38.0 35.0 40.0 30.0 41.0 36-37 36.26557665007036 38.0 35.0 40.0 30.0 41.0 38-39 36.302301466433555 38.0 35.0 40.0 30.0 41.0 40-41 36.180195125348845 38.0 35.0 40.0 30.0 41.0 42-43 36.03159312064386 38.0 35.0 40.0 29.5 41.0 44-45 35.83702419951886 38.0 35.0 40.0 28.5 41.0 46-47 35.52693098418055 38.0 34.5 40.0 27.5 41.0 48-49 35.22472225570006 38.0 34.0 40.0 27.0 41.0 50-51 34.98724461845963 38.0 34.0 40.0 26.0 41.0 52-53 34.71036724229414 38.0 33.0 40.0 26.0 41.0 54-55 34.440848446619725 38.0 33.0 40.0 24.5 41.0 56-57 33.66661879770918 37.5 32.5 40.0 21.5 41.0 58-59 30.560775837106764 35.5 27.0 39.0 8.0 40.5 60-61 30.285409072197865 34.5 26.0 39.0 10.5 40.0 62-63 32.08805449078646 35.5 30.5 39.0 18.5 40.0 64-65 32.0991102634697 36.0 31.0 39.0 16.0 40.0 66-67 27.190234693542152 32.0 18.5 37.0 4.0 39.5 68-69 19.712464606971075 20.0 8.0 32.0 2.0 37.0 70-71 17.786170932090776 15.5 7.5 29.0 2.0 35.0 72-73 19.47841944789223 20.0 7.5 31.0 2.0 35.5 74-75 20.910386376833824 23.5 9.0 32.5 2.0 36.0 76-77 22.720100031773512 27.5 11.5 33.0 2.0 35.5 78-79 24.901884028254948 30.5 13.0 34.5 2.0 36.0 80-81 26.52678639905963 32.0 22.5 35.0 2.0 36.0 82-83 27.687137950248633 33.0 26.5 35.0 2.0 36.0 84-85 27.75420490313775 34.0 27.0 35.0 2.0 36.0 86-87 27.45833228986832 33.5 26.5 35.0 2.0 36.0 88-89 26.991480825547782 33.0 26.0 35.0 2.0 35.0 90-91 25.14513920084145 32.0 16.0 34.5 2.0 35.0 92-93 23.539192741553023 31.0 2.0 34.0 2.0 35.0 94-95 19.80999695569082 27.5 2.0 34.0 2.0 35.0 96-97 17.108184104793104 18.0 2.0 33.0 2.0 35.0 98-99 17.37419666237281 21.5 2.0 33.0 2.0 35.0 100-101 16.594078349092264 10.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.24913829254449382 1101 2 0.23423176954117153 1101 3 0.23072449273831452 1101 4 0.22250316991668484 1101 5 0.3337738788232798 1101 6 0.3033587639885127 1101 7 0.38346041716449975 1101 8 0.4087428609249599 1101 9 0.40950183528198636 1101 10-11 0.2641706227039151 1101 12-13 0.31967637489159983 1101 14-15 0.4329784132266852 1101 16-17 0.4488567521629747 1101 18-19 0.4681965607563896 1101 20-21 0.5377158582742183 1101 22-23 0.49394012152442457 1101 24-25 0.43924140682809565 1101 26-27 0.48103148941098794 1101 28-29 0.48868637004225945 1101 30-31 0.42212709612016397 1101 32-33 0.4794890714748732 1101 34-35 0.3731073169644006 1101 36-37 0.24629247398048193 1101 38-39 0.2939031506547636 1101 40-41 0.42508177310220674 1101 42-43 0.5225015799788437 1101 44-45 0.3440559455585728 1101 46-47 0.43982118398879777 1101 48-49 0.3191449377062625 1101 50-51 0.3178174194488932 1101 52-53 0.3572803260684978 1101 54-55 0.38759525858971955 1101 56-57 -3.1068212102223 1101 58-59 -9.864101717271797 1101 60-61 -11.830239266095411 1101 62-63 -3.9534331981215978 1101 64-65 -1.2371975851566788 1101 66-67 -2.2106860478857975 1101 68-69 -4.369652021457014 1101 70-71 -4.294250219742279 1101 72-73 -3.4823396011446555 1101 74-75 -6.546977319773523 1101 76-77 -5.090856400085393 1101 78-79 1.0946350103053923 1101 80-81 1.9026495021522614 1101 82-83 0.5546646731804152 1101 84-85 0.1892306642925945 1101 86-87 0.17640854755538982 1101 88-89 -0.030719014813389123 1101 90-91 1.3668424859709702 1101 92-93 0.9943411101736324 1101 94-95 -8.055897469996285 1101 96-97 -9.257734413784418 1101 98-99 -8.422833707835817 1101 100-101 -9.476891357981437 1102 1 0.33797345692371294 1102 2 0.3354619834282708 1102 3 0.36424463523092854 1102 4 0.22247071787605677 1102 5 0.3245477192185149 1102 6 0.23143718827152782 1102 7 0.3447126806572882 1102 8 0.40930699297364725 1102 9 0.3914753935371067 1102 10-11 0.45733065127356554 1102 12-13 0.4239938299403292 1102 14-15 0.4745325103338658 1102 16-17 0.4509496124176735 1102 18-19 0.46730931603836723 1102 20-21 0.413428099065392 1102 22-23 0.40181418686119486 1102 24-25 0.3462002208912338 1102 26-27 0.19100745415533282 1102 28-29 0.3869200306620755 1102 30-31 0.4687128154440927 1102 32-33 0.2777560732323252 1102 34-35 0.3233323325976869 1102 36-37 0.06406085532011474 1102 38-39 0.2066067909142575 1102 40-41 0.20513451137279048 1102 42-43 0.44295288561779955 1102 44-45 0.2603505745235779 1102 46-47 0.24010028635646563 1102 48-49 0.3261956992178412 1102 50-51 0.2844495348181155 1102 52-53 0.3320150787957985 1102 54-55 0.07676553844782319 1102 56-57 -3.488045259703508 1102 58-59 -9.90269815910559 1102 60-61 -7.820272785052445 1102 62-63 -2.1194382037377295 1102 64-65 -0.5815089943457927 1102 66-67 -1.8256554777671603 1102 68-69 -4.782439903657588 1102 70-71 -4.687898881206742 1102 72-73 -3.2147079925607187 1102 74-75 -3.930785085146752 1102 76-77 -4.014831271240418 1102 78-79 1.9182832864944892 1102 80-81 2.387607907230784 1102 82-83 0.9541745584572645 1102 84-85 0.722682939047278 1102 86-87 0.5730385735466577 1102 88-89 0.33853193577948915 1102 90-91 1.7450254532433114 1102 92-93 1.5510560152921649 1102 94-95 -8.465914851842703 1102 96-97 -9.39107214320849 1102 98-99 -8.45361261608397 1102 100-101 -9.379450401431608 1103 1 0.19145336087206388 1103 2 0.1834090762913192 1103 3 0.20839690071138506 1103 4 0.16203887911685655 1103 5 0.1208040668939887 1103 6 0.26858043906867834 1103 7 0.19321088953025622 1103 8 0.3063250617056852 1103 9 0.28583826406421053 1103 10-11 0.26312130592064875 1103 12-13 0.2566435169851644 1103 14-15 0.31900265165575803 1103 16-17 0.30049395757375663 1103 18-19 0.376724542586409 1103 20-21 0.3312685116150931 1103 22-23 0.3260131003753557 1103 24-25 0.4322786368263607 1103 26-27 0.42109248348257466 1103 28-29 0.3715399574423799 1103 30-31 0.35305718421943766 1103 32-33 0.2666884827902436 1103 34-35 0.28820360764394337 1103 36-37 0.39145624169180593 1103 38-39 0.3400900409241032 1103 40-41 0.32836700359811744 1103 42-43 0.1753840945301306 1103 44-45 0.09568892038823407 1103 46-47 0.10147787955411758 1103 48-49 0.12679110133570504 1103 50-51 -0.026032494863414968 1103 52-53 0.19147360731605545 1103 54-55 0.19424870972679997 1103 56-57 0.7124527129768836 1103 58-59 -8.148869871108111 1103 60-61 -7.814543146820739 1103 62-63 -2.1827584808703513 1103 64-65 -0.3882759116390275 1103 66-67 -1.849676262713821 1103 68-69 -3.1628811901198155 1103 70-71 -3.9513333180842736 1103 72-73 -2.9730957814045595 1103 74-75 -3.8911185103327526 1103 76-77 -0.5666529064803498 1103 78-79 3.1508079883013593 1103 80-81 2.5952723563116002 1103 82-83 1.1093599696291747 1103 84-85 0.7186381411887588 1103 86-87 0.6523540087314821 1103 88-89 0.5024958376808435 1103 90-91 1.9055133877392372 1103 92-93 1.6179221334671858 1103 94-95 -7.420460574788811 1103 96-97 -8.861573658777518 1103 98-99 -8.024102322024213 1103 100-101 -9.286917039230293 1104 1 0.2071305044721612 1104 2 0.1651135772014385 1104 3 0.17902807269783594 1104 4 0.0998772624063946 1104 5 0.22709987425398737 1104 6 0.08323746360775885 1104 7 0.11564352890466978 1104 8 0.22667964996824708 1104 9 0.2540720259632394 1104 10-11 0.21291679974332567 1104 12-13 0.24984896072503915 1104 14-15 0.2265260505223594 1104 16-17 0.07058585090121028 1104 18-19 0.20570018758342457 1104 20-21 0.21247501212268816 1104 22-23 0.28182318823787966 1104 24-25 0.24540068684485306 1104 26-27 0.15297399021378766 1104 28-29 0.15609296299290065 1104 30-31 0.16766971473457204 1104 32-33 0.36261787562563086 1104 34-35 0.13746714145080574 1104 36-37 0.05157276890690099 1104 38-39 0.03183447279501905 1104 40-41 -0.06199722642239891 1104 42-43 0.007651604245353383 1104 44-45 0.11361224983862428 1104 46-47 0.04390469173610967 1104 48-49 0.14521772611607986 1104 50-51 0.11896451946282838 1104 52-53 0.004849453464863984 1104 54-55 0.09068325527509558 1104 56-57 0.42538267633376137 1104 58-59 -8.375032163129735 1104 60-61 -7.478665245075742 1104 62-63 -2.1227766555009424 1104 64-65 -0.4678586713552164 1104 66-67 -1.9375849493334023 1104 68-69 -3.6312008906320656 1104 70-71 -3.7371708286810605 1104 72-73 -2.978853391268757 1104 74-75 -3.744626025525868 1104 76-77 -0.30789756373221167 1104 78-79 3.516141488337123 1104 80-81 2.9264514950736285 1104 82-83 1.4150900514150102 1104 84-85 0.926908291159954 1104 86-87 0.8282381923118827 1104 88-89 0.8349234427158443 1104 90-91 2.1404903478742554 1104 92-93 1.8139597535109537 1104 94-95 -6.267442438414767 1104 96-97 -7.732107831019569 1104 98-99 -5.524607003460863 1104 100-101 -4.636850062556251 1105 1 0.28557000289805146 1105 2 0.28410088909673803 1105 3 0.29201713942736873 1105 4 0.25207921709522196 1105 5 0.2787120081340646 1105 6 0.3312193630717317 1105 7 0.3240656354248088 1105 8 0.24324889140725503 1105 9 0.2940135790601488 1105 10-11 0.32503977042464527 1105 12-13 0.28184319233186983 1105 14-15 0.40769587505815963 1105 16-17 0.502635356767442 1105 18-19 0.5864934527964039 1105 20-21 0.45948288801852755 1105 22-23 0.5300107194737365 1105 24-25 0.5750604592323683 1105 26-27 0.5449145283940524 1105 28-29 0.5675097871710832 1105 30-31 0.6130035682424193 1105 32-33 0.6042651912699171 1105 34-35 0.5326647853511943 1105 36-37 0.48176767791158426 1105 38-39 0.5145827424554668 1105 40-41 0.525755737707783 1105 42-43 0.5964373928874593 1105 44-45 0.5008959865542053 1105 46-47 0.5456424414480026 1105 48-49 0.44835882175948427 1105 50-51 0.4812711659043529 1105 52-53 0.5229199372024596 1105 54-55 0.45013651216125794 1105 56-57 0.9639274154126412 1105 58-59 -8.224359170204238 1105 60-61 -7.76778790754954 1105 62-63 -2.036216520317776 1105 64-65 -0.13538015874327414 1105 66-67 -1.3350566185309525 1105 68-69 -3.016395581448883 1105 70-71 -3.8722980733744983 1105 72-73 -2.7226703039296645 1105 74-75 -3.1469667833760333 1105 76-77 0.39919757633204966 1105 78-79 4.232006065303782 1105 80-81 3.6116409164476444 1105 82-83 2.0445801587222725 1105 84-85 1.6096838548874146 1105 86-87 1.591396018436189 1105 88-89 1.5710124567783978 1105 90-91 2.7220031390349817 1105 92-93 2.3311850680239594 1105 94-95 -6.139148727906427 1105 96-97 -7.673162457851076 1105 98-99 -5.49891510778156 1105 100-101 -4.871032814517266 1106 1 0.08119074267402482 1106 2 0.13559778182585092 1106 3 0.028979177077619767 1106 4 0.08973623700007494 1106 5 0.0748779310362977 1106 6 0.12599291965675974 1106 7 0.24050182177399648 1106 8 0.20779561223650944 1106 9 0.12152896461925167 1106 10-11 0.15752451041459636 1106 12-13 0.12289802001374994 1106 14-15 0.14569246421783788 1106 16-17 0.21022041659361435 1106 18-19 0.13441859772027698 1106 20-21 0.18458756795509146 1106 22-23 0.11236967159219091 1106 24-25 0.13539524124713864 1106 26-27 0.15229465668062403 1106 28-29 0.3308752005625024 1106 30-31 0.2252567739396767 1106 32-33 0.3152337981380242 1106 34-35 0.2632114027389463 1106 36-37 0.24813828534929883 1106 38-39 0.3508596780159081 1106 40-41 0.28304655558209646 1106 42-43 0.3117622108106204 1106 44-45 0.4119044961873044 1106 46-47 0.29416945762596214 1106 48-49 0.23420427114526632 1106 50-51 0.15728050184127795 1106 52-53 0.2875777552990755 1106 54-55 0.34793214368188075 1106 56-57 1.0315554375796907 1106 58-59 -8.18434334283528 1106 60-61 -7.52975317604097 1106 62-63 -1.8437517289809868 1106 64-65 -0.054986458740284405 1106 66-67 0.8617682049190982 1106 68-69 -1.9600082254942457 1106 70-71 -2.2577729074075172 1106 72-73 -0.9343445101355279 1106 74-75 -0.8806790792547652 1106 76-77 2.376149567328543 1106 78-79 4.431088451822109 1106 80-81 3.5126966611834867 1106 82-83 1.982087434085333 1106 84-85 1.4688854338578388 1106 86-87 1.4833972198860614 1106 88-89 1.2137963269453884 1106 90-91 2.4570514406223722 1106 92-93 1.8374127105688771 1106 94-95 -5.978792766311884 1106 96-97 -7.452691220389093 1106 98-99 -5.337371820582501 1106 100-101 -4.936753514540587 1107 1 0.11057018070125935 1107 2 0.13931540095178363 1107 3 0.15138419489485955 1107 4 0.12790024857122262 1107 5 0.15299896694638448 1107 6 0.12186809518724573 1107 7 0.09991738324376342 1107 8 0.1692874533671116 1107 9 0.28763614699741424 1107 10-11 0.11886010920618162 1107 12-13 0.044871504372878235 1107 14-15 0.09256706300768514 1107 16-17 0.12627132100229232 1107 18-19 0.14920377218599157 1107 20-21 0.21395678576946864 1107 22-23 0.32364097656107305 1107 24-25 0.22390422174149904 1107 26-27 0.18650244389499449 1107 28-29 0.17759198056219816 1107 30-31 0.1126559461959431 1107 32-33 0.12141374041586062 1107 34-35 0.08402495305786317 1107 36-37 0.02865908067690981 1107 38-39 0.08391707005539217 1107 40-41 0.04248098102645059 1107 42-43 0.08617465878940322 1107 44-45 -0.07646196243680237 1107 46-47 0.0646626209715464 1107 48-49 0.04582544138769862 1107 50-51 0.06755765397429059 1107 52-53 0.19456927599593854 1107 54-55 0.3352015659940335 1107 56-57 0.8474159921939091 1107 58-59 -8.451928020638729 1107 60-61 -7.692708858926871 1107 62-63 -1.9996360933512172 1107 64-65 -0.11247722681532935 1107 66-67 0.7957931238020031 1107 68-69 -2.3578606047397592 1107 70-71 -2.2334496691951973 1107 72-73 -0.8385873947632163 1107 74-75 -0.6571022372150317 1107 76-77 2.5603762519168747 1107 78-79 4.62736095983696 1107 80-81 3.4788852579108536 1107 82-83 1.993309161002049 1107 84-85 1.5666672201559955 1107 86-87 1.3435392180539019 1107 88-89 1.2102118377816886 1107 90-91 2.4426081422965 1107 92-93 1.9194400053506762 1107 94-95 -7.244148970121364 1107 96-97 -8.368884606588761 1107 98-99 -6.06707137170808 1107 100-101 -5.387385811722465 1108 1 -0.08594508808452872 1108 2 -0.11184371309906993 1108 3 -0.05161760976400842 1108 4 -0.08720338612717882 1108 5 -0.0017781170406081515 1108 6 0.0032731975797375412 1108 7 -0.06597248295597069 1108 8 -0.043634542881157756 1108 9 -0.10013146760274338 1108 10-11 -0.1113357329969773 1108 12-13 -0.143666653880274 1108 14-15 -0.18804979863465832 1108 16-17 -0.2120594133971423 1108 18-19 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3.1051989220209784 2312 94-95 5.139117796595471 2312 96-97 0.03408398731219009 2312 98-99 -1.2920410805112645 2312 100-101 -0.5416346497998372 2313 1 -0.2858116439796312 2313 2 -0.4161982987016799 2313 3 -0.3748551839963383 2313 4 -0.29076394691631435 2313 5 -0.3516883175792387 2313 6 -0.323613036462433 2313 7 -0.2263629041308306 2313 8 -0.37370464962024386 2313 9 -0.5316806338200095 2313 10-11 -0.3876152336180354 2313 12-13 -0.3600509664244953 2313 14-15 -0.5157093283089651 2313 16-17 -0.42572221115644027 2313 18-19 -0.4253743809585231 2313 20-21 -0.5238912254618597 2313 22-23 -0.40625788621559167 2313 24-25 -0.3599666709848819 2313 26-27 -0.3948689304937716 2313 28-29 -0.3684436285722512 2313 30-31 -0.5573280542361374 2313 32-33 -0.6211041767372052 2313 34-35 -0.6732496492627931 2313 36-37 -0.7798925302133952 2313 38-39 -0.759988184796832 2313 40-41 -0.6220556397552883 2313 42-43 -0.6212556695749427 2313 44-45 -0.7325153865156224 2313 46-47 -0.764313537959417 2313 48-49 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26-27 -0.5769626811198805 2315 28-29 -0.6008588988169805 2315 30-31 -0.5255915248327696 2315 32-33 -0.5302204811697777 2315 34-35 -0.4838748473535901 2315 36-37 -0.4140171686411165 2315 38-39 -0.5797829500581813 2315 40-41 -0.5929838162868037 2315 42-43 -0.5020094151656522 2315 44-45 -0.5459935748631821 2315 46-47 -0.5386138625567511 2315 48-49 -0.5622168559629657 2315 50-51 -0.5048469657054042 2315 52-53 -0.4702683151333886 2315 54-55 -0.37178239717149353 2315 56-57 0.28717349046090845 2315 58-59 3.524121163518668 2315 60-61 3.502119707726038 2315 62-63 0.6603266825858505 2315 64-65 -0.15039727522994895 2315 66-67 -1.821578016794927 2315 68-69 -4.78827315368258 2315 70-71 -3.7237103746008273 2315 72-73 -3.020189637540909 2315 74-75 -0.51294675243604 2315 76-77 1.9491278305027429 2315 78-79 3.6044941337666216 2315 80-81 2.599003435940592 2315 82-83 0.8238193229226631 2315 84-85 0.47796819557558123 2315 86-87 0.5825286212691871 2315 88-89 0.549494041884504 2315 90-91 2.082765578574133 2315 92-93 3.631791794558385 2315 94-95 6.369530961781631 2315 96-97 4.025628636031424 2315 98-99 6.436847351189005 2315 100-101 6.85750285469682 2316 1 -0.463923908419158 2316 2 -0.46919222953382445 2316 3 -0.6277400434468987 2316 4 -0.3712488516065662 2316 5 -0.5924224384647658 2316 6 -0.6303377992149848 2316 7 -0.5621492588122479 2316 8 -0.5996356644888081 2316 9 -0.6225249865515963 2316 10-11 -0.6862525413109637 2316 12-13 -0.6061825365722484 2316 14-15 -0.8229332749909659 2316 16-17 -0.8907059014114012 2316 18-19 -0.9173555099639827 2316 20-21 -0.7828165174598993 2316 22-23 -0.9860239783274949 2316 24-25 -0.8447706648492854 2316 26-27 -0.826961373217479 2316 28-29 -0.8770992200278442 2316 30-31 -0.810853133961281 2316 32-33 -0.6537552879402782 2316 34-35 -0.5548194593064224 2316 36-37 -0.6570820473263197 2316 38-39 -0.8163628126960489 2316 40-41 -0.7358071316275527 2316 42-43 -0.7310506529545151 2316 44-45 -0.796863080832324 2316 46-47 -0.8923126479015693 2316 48-49 -0.7227026457902781 2316 50-51 -0.8972906156648861 2316 52-53 -0.8020533826747211 2316 54-55 -0.6913814889640761 2316 56-57 0.2260397035055135 2316 58-59 3.335472249115032 2316 60-61 3.3399351395293877 2316 62-63 0.6853615537549693 2316 64-65 0.2805945557362435 2316 66-67 -1.9851556878866212 2316 68-69 -2.9427714909026683 2316 70-71 -3.1065946282282635 2316 72-73 -0.8807155488312475 2316 74-75 5.499685716278311 2316 76-77 5.846238076211517 2316 78-79 4.827589771824936 2316 80-81 3.2670621052034647 2316 82-83 1.4463655423249087 2316 84-85 1.1510241730507644 2316 86-87 1.0678977399746543 2316 88-89 1.048354485211096 2316 90-91 2.456490280316334 2316 92-93 4.00902784054772 2316 94-95 6.580826891265513 2316 96-97 3.6818557710948916 2316 98-99 6.094499788458894 2316 100-101 6.126047166803682 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 9.0 4 45.0 5 98.0 6 243.0 7 608.0 8 1382.0 9 2313.0 10 3680.0 11 5781.0 12 8349.0 13 11645.0 14 14578.0 15 19406.0 16 26223.0 17 34589.0 18 37787.0 19 36425.0 20 38606.0 21 39266.0 22 39717.0 23 39967.0 24 42388.0 25 44808.0 26 55452.0 27 78280.0 28 112055.0 29 148551.0 30 179706.0 31 200756.0 32 209826.0 33 208754.0 34 213586.0 35 228810.0 36 229636.0 37 172683.0 38 65573.0 39 4001.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.13926946000911 19.620522190023063 14.4451985414876 22.795009808480227 2 15.070575325482412 21.74259299553396 39.478194987328216 23.70863669165541 3 19.39044069770354 26.32263310460544 30.67885853096592 23.608067666725102 4 12.63924388438199 17.19056436326982 37.872614834629054 32.297576917719134 5 12.98704044643074 38.77583942651881 34.36517451817697 13.871945608873485 6 30.288058951599396 37.045134035689635 18.05557077275367 14.6112362399573 7 27.230680375710016 34.009316055639644 21.171096436514805 17.58890713213554 8 25.358479284798364 34.85473200481925 20.363149444726396 19.423639265655993 9 26.448903344357543 17.022305629858963 20.63955535868849 35.88923566709501 10-11 24.30192190603493 26.63817485447576 27.95509291795078 21.104810321538526 12-13 26.51284166305367 24.118226412082073 27.90706091905268 21.461871005811577 14-15 23.32381297843892 25.114680559419654 27.010260305207005 24.551246156934422 16-17 21.619471060959565 28.679961065704067 27.805250260458703 21.895317612877665 18-19 21.440019784189552 27.9861174154085 29.674288692646982 20.899574107754965 20-21 22.450273674786388 26.922942978289143 28.884370250603773 21.74241309632069 22-23 22.070830665975894 26.824061624956762 28.36527249306226 22.739835216005083 24-25 22.26385650883985 26.909170734226905 28.959620220176507 21.867352536756737 26-27 22.62262405229643 26.474422397528862 29.1073806765612 21.7955728736135 28-29 21.866108251229814 26.57500916138098 29.79813266305742 21.760749924331787 30-31 21.817829985949654 27.35604463160844 29.278208349320384 21.547917033121518 32-33 21.65807324134142 27.328540424709306 28.779404893556578 22.2339814403927 34-35 22.264326735720417 26.594556817712693 28.76487622701298 22.376240219553907 36-37 22.673834552719914 26.474611811307025 29.04113956150647 21.81041407446659 38-39 22.923387416543584 26.175331265455483 29.453709383989484 21.447571934011446 40-41 22.132512980381826 26.451643855332506 29.823654793807012 21.592188370478652 42-43 21.723350165336935 27.254882070880843 29.04709292511449 21.97467483866773 44-45 22.051684262267475 27.44997933961174 28.296526508942716 22.20180988917807 46-47 21.94661813900762 27.182741062079753 27.661881851494947 23.208758947417685 48-49 22.484947110516824 27.08454935513071 27.895861476018275 22.534642058334192 50-51 22.80741428191031 27.350962517425558 27.89937816205379 21.94224503861034 52-53 22.35612119584252 27.333571260907163 28.31973080641982 21.9905767368305 54-55 21.515602097192467 27.087052592676113 28.74848489119405 22.64886041893737 56-57 22.067350228353146 28.116305336750163 27.729631128468053 22.086713306428642 58-59 21.18357614996289 26.914844630656077 29.401096975131562 22.50048224424947 60-61 21.511741811670078 26.653537541484933 29.327320790778515 22.507399856066467 62-63 23.18295378928707 26.62544969175314 28.047284670850193 22.144311848109595 64-65 22.61442271721964 26.77345233306359 28.511765101795174 22.1003598479216 66-67 21.745952876492957 26.982300300302676 29.17851334548891 22.09323347771545 68-69 21.276484488712004 26.852554361382108 30.045528627940456 21.825432521965435 70-71 22.01175322749059 26.87905320399535 28.766283672251642 22.342909896262412 72-73 21.38227577785259 26.71171395503107 28.86583624009195 23.04017402702439 74-75 21.647140325509255 26.94908540312946 28.818584857298156 22.585189414063127 76-77 20.909615364526722 26.86395484444073 29.414578545945425 22.811851245087126 78-79 21.712139963442972 26.882893553724863 28.71715917654211 22.687807306290054 80-81 21.42922571048305 27.923471384952634 28.10410631801456 22.543196586549755 82-83 21.701887218452594 27.81077846513902 27.41709192782166 23.070242388586728 84-85 21.818353280367763 27.07252560544961 27.319281746326002 23.789839367856626 86-87 22.220976570264945 26.98600617634675 27.888353845503676 22.904663407884623 88-89 22.59758274760696 26.408115550729526 27.991000112498593 23.003301589164916 90-91 21.717937876230646 26.90094502031886 28.624563775621375 22.756553327829124 92-93 21.27507300746107 27.46922606805834 28.184423169606344 23.071277754874245 94-95 20.34355952298893 27.508107169682827 28.48272534416093 23.66560796316731 96-97 20.12594016032328 27.06832287511783 28.27010102701818 24.535635937540714 98-99 20.92056833023311 26.708858840321682 28.010637436949498 24.359935392495707 100-101 20.602900384810777 27.04760533107932 28.57988335656994 23.76961092753996 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 735.0 1 559.5 2 391.5 3 440.5 4 486.0 5 555.0 6 726.5 7 1099.5 8 1658.5 9 2097.5 10 2316.0 11 2536.5 12 2727.5 13 2913.0 14 3235.0 15 3513.0 16 3759.5 17 4055.0 18 4367.5 19 4761.5 20 5247.5 21 5967.5 22 6864.0 23 7949.5 24 9320.5 25 11340.5 26 14138.5 27 17255.0 28 20677.5 29 24547.5 30 28732.5 31 33578.5 32 38799.5 33 44252.0 34 50229.0 35 55943.5 36 61799.5 37 67079.5 38 70569.5 39 73185.0 40 75431.5 41 78760.0 42 82451.5 43 86206.5 44 91308.0 45 96661.0 46 103598.0 47 114819.0 48 132259.5 49 130440.0 50 112379.0 51 101226.0 52 91577.0 53 84431.0 54 77660.0 55 70271.0 56 62850.0 57 55036.0 58 47806.0 59 40624.0 60 33090.5 61 26058.5 62 20102.5 63 15386.5 64 11378.0 65 8089.5 66 5599.0 67 3916.5 68 2584.5 69 1471.5 70 865.0 71 504.0 72 291.5 73 178.0 74 98.0 75 58.0 76 36.0 77 18.5 78 8.0 79 4.0 80 2.0 81 1.5 82 2.0 83 2.0 84 2.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025669239329657204 2 0.011895501152767973 3 1.5651975201010491E-4 4 0.0 5 0.06425135820014807 6 0.0 7 0.0 8 3.912993800252623E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 4.3042931802778853E-4 16-17 1.9564969001263114E-5 18-19 4.6955925603031477E-4 20-21 0.0 22-23 0.0 24-25 7.825987600505246E-5 26-27 9.782484500631556E-5 28-29 9.195535430593664E-4 30-31 0.005262976661339778 32-33 0.005712970948368829 34-35 0.0016630223651073648 36-37 0.08823801019569665 38-39 0.08156635576626592 40-41 0.08669237764459686 42-43 0.09066406635185327 44-45 0.09363794164004527 46-47 0.08872713442072823 48-49 0.07859248047807393 50-51 0.073094724188719 52-53 0.06219703645501544 54-55 0.05149499841132452 56-57 0.06880999597744238 58-59 0.7033019406884051 60-61 0.33569573812367254 62-63 0.003423869575221045 64-65 0.0025043160321616786 66-67 1.0880079261602418 68-69 2.344098501010335 70-71 2.3218726962249003 72-73 1.7856164608692795 74-75 1.2667339179867803 76-77 0.7339798120823857 78-79 0.5616320001502589 80-81 0.038660378746495916 82-83 5.8694907003789346E-5 84-85 0.0011152032330719976 86-87 0.0012521580160808393 88-89 1.9564969001263114E-5 90-91 0.33053058630733906 92-93 0.05466452338952914 94-95 0.9403902350457115 96-97 1.6377639901267342 98-99 0.6360375772620626 100-101 0.5610841810182235 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2555588.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.21458908870314 #Duplication Level Percentage of deduplicated Percentage of total 1 69.35309586360661 29.27712443911591 2 15.356427979496079 12.965305940493819 3 5.888253827575452 7.457106473432436 4 2.942738561484849 4.969059966742572 5 1.6796842368245075 3.5453589928159257 6 1.0553979692533029 2.6731914958247964 7 0.696224412291889 2.0573579234898154 8 0.4881556510835103 1.6485832177454987 9 0.3801121405486815 1.444165003880097 >10 1.9064342780820513 14.684081006269981 >50 0.1530010974740551 4.470136883718645 >100 0.09019357599917777 7.152949007589336 >500 0.006390522822562234 1.8707018618335485 >1k 0.0029637207293039328 2.2991722299616595 >5k 6.483139095352353E-4 1.860771076770487 >10k+ 2.778488183722437E-4 1.6249344803155552 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 16468 0.6443918190256019 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 14416 0.5640971862444181 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10677 0.41779034805297255 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 9135 0.35745198365307707 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8311 0.3252089147389955 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 6547 0.2561837041025392 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6511 0.25477502633444826 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6033 0.2360709159692407 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5957 0.23309704068104875 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5099 0.19952355387488122 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4255 0.16649788620074912 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3624 0.14180689532115504 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2973 0.11633330568151048 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2960 0.11582461648747763 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2767 0.10827253845299009 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2679 0.10482910390876776 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2581 0.10099436998452019 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.912993800252623E-5 0.0 5 0.0 0.0 0.0 3.912993800252623E-5 0.0 6 0.0 0.0 0.0 3.912993800252623E-5 0.0 7 0.0 0.0 0.0 3.912993800252623E-5 0.0 8 0.0 0.0 0.0 3.912993800252623E-5 0.0 9 0.0 0.0 0.0 3.912993800252623E-5 0.0 10-11 0.0 0.0 0.0 3.912993800252623E-5 0.0 12-13 0.0 0.0 0.0 3.912993800252623E-5 0.0 14-15 0.0 0.0 0.0 5.869490700378934E-5 0.0 16-17 0.0 0.0 0.0 7.825987600505246E-5 0.0 18-19 0.0 0.0 0.0 7.825987600505246E-5 0.0 20-21 0.0 0.0 0.0 1.1738981400757868E-4 0.0 22-23 0.0 0.0 0.0 2.9347453501894674E-4 0.0 24-25 0.0 0.0 0.0 6.456439770416828E-4 0.0 26-27 0.0 0.0 0.0 0.0010956382640707344 0.0 28-29 0.0 0.0 0.0 0.003091265102199572 0.0 30-31 0.0 0.0 0.0 0.008334676794538087 0.0 32-33 0.0 0.0 0.0 0.021071471614360376 0.0 34-35 0.0 0.0 0.0 0.04153642918968159 0.0 36-37 0.0 0.0 0.0 0.07194039101764446 0.0 38-39 0.0 0.0 0.0 0.11447463362639049 0.0 40-41 0.0 0.0 0.0 0.16293706184251922 0.0 42-43 0.0 0.0 0.0 0.2098342925385469 0.0 44-45 0.0 0.0 0.0 0.26737486637126173 0.0 46-47 0.0 0.0 0.0 0.33927612745090363 0.0 48-49 0.0 0.0 0.0 0.4126838911436429 0.0 50-51 0.0 0.0 0.0 0.4843895025332722 0.0 52-53 0.0 0.0 0.0 0.5531799335417134 0.0 54-55 0.0 0.0 0.0 0.6305006910347051 0.0 56-57 0.0 0.0 0.0 0.7047497483944987 0.0 58-59 0.0 0.0 0.0 0.7673967791365431 3.912993800252623E-5 60-61 0.0 3.912993800252623E-5 1.9564969001263114E-5 0.8236656299841758 3.912993800252623E-5 62-63 1.9564969001263114E-5 5.869490700378934E-5 3.912993800252623E-5 0.8714041543472578 3.912993800252623E-5 64-65 3.912993800252623E-5 7.825987600505246E-5 3.912993800252623E-5 0.9225078533785571 3.912993800252623E-5 66-67 3.912993800252623E-5 7.825987600505246E-5 3.912993800252623E-5 0.9653942654293259 3.912993800252623E-5 68-69 3.912993800252623E-5 7.825987600505246E-5 3.912993800252623E-5 1.0173001281896767 3.912993800252623E-5 70-71 3.912993800252623E-5 7.825987600505246E-5 3.912993800252623E-5 1.1021142688101526 3.912993800252623E-5 72-73 3.912993800252623E-5 7.825987600505246E-5 3.912993800252623E-5 1.220071466918768 3.912993800252623E-5 74-75 3.912993800252623E-5 7.825987600505246E-5 3.912993800252623E-5 1.369626089964423 3.912993800252623E-5 76-77 3.912993800252623E-5 7.825987600505246E-5 3.912993800252623E-5 1.5570193630585212 3.912993800252623E-5 78-79 3.912993800252623E-5 7.825987600505246E-5 3.912993800252623E-5 1.8051227349635388 3.912993800252623E-5 80-81 3.912993800252623E-5 7.825987600505246E-5 3.912993800252623E-5 2.098792919672498 3.912993800252623E-5 82-83 3.912993800252623E-5 7.825987600505246E-5 3.912993800252623E-5 2.3917000705904083 3.912993800252623E-5 84-85 3.912993800252623E-5 9.782484500631557E-5 3.912993800252623E-5 2.676722539000809 3.912993800252623E-5 86-87 3.912993800252623E-5 1.1738981400757869E-4 3.912993800252623E-5 2.9480886590483286 3.912993800252623E-5 88-89 3.912993800252623E-5 1.1738981400757869E-4 3.912993800252623E-5 3.194274663991222 3.912993800252623E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14890 0.0 23.805405 1 TACATGG 14930 0.0 23.26196 2 ACATGGG 14860 0.0 22.511072 3 AGTACTT 8305 0.0 20.838057 12-13 CATGGGG 4155 0.0 20.780157 4 GAGTACT 8085 0.0 20.442724 12-13 ATGGGAG 3535 0.0 20.0203 5 CATGGGA 10920 0.0 19.529205 4 TACCTGG 1995 0.0 19.395422 2 GTACTTT 9100 0.0 18.525309 14-15 ATGGGAT 3930 0.0 17.888027 5 GCTTATA 2815 0.0 17.772879 1 GTACCTG 2430 0.0 16.704077 1 ATGGGGA 2545 0.0 16.499508 5 TACTTTT 10450 0.0 16.425398 14-15 ACTTTTT 10610 0.0 16.28881 16-17 TAGGACA 1030 0.0 16.032436 4 GCGTTTA 1030 0.0 15.757604 62-63 TACGCGG 155 1.6319482E-6 15.673687 64-65 CGTTGAG 865 0.0 15.402783 62-63 >>END_MODULE