##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939565_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1426027 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48236323716171 31.0 31.0 34.0 30.0 34.0 2 31.672382079722194 31.0 31.0 34.0 30.0 34.0 3 31.696016274586665 31.0 31.0 34.0 30.0 34.0 4 35.4917901273959 37.0 35.0 37.0 33.0 37.0 5 35.24352273834928 37.0 35.0 37.0 33.0 37.0 6 35.29538641273973 37.0 35.0 37.0 32.0 37.0 7 35.22540106183123 37.0 35.0 37.0 32.0 37.0 8 35.201796319424524 37.0 35.0 37.0 32.0 37.0 9 36.756905724786414 39.0 35.0 39.0 32.0 39.0 10-11 36.64845125653301 39.0 35.0 39.0 32.0 39.0 12-13 36.59521523786015 39.0 35.0 39.0 32.0 39.0 14-15 37.502996787578354 39.0 36.0 41.0 32.0 41.0 16-17 37.499801546534535 39.0 36.0 41.0 32.0 41.0 18-19 37.58619437079382 39.0 36.0 41.0 32.0 41.0 20-21 37.5557173882402 39.0 36.0 41.0 32.0 41.0 22-23 37.41580559133874 39.0 36.0 41.0 32.0 41.0 24-25 37.26107500068372 39.0 36.0 41.0 31.0 41.0 26-27 36.93884793205177 39.0 36.0 40.0 31.0 41.0 28-29 36.72798937187024 39.0 35.0 40.0 30.0 41.0 30-31 36.43881216835305 38.0 35.0 40.0 30.0 41.0 32-33 36.16129533311782 38.0 35.0 40.0 30.0 41.0 34-35 36.061593153565816 38.0 35.0 40.0 30.0 41.0 36-37 35.849868200251464 38.0 35.0 40.0 30.0 41.0 38-39 35.786286304537015 38.0 35.0 40.0 29.0 41.0 40-41 35.66344010316775 38.0 35.0 40.0 29.0 41.0 42-43 35.423312111201255 38.0 34.5 40.0 27.5 41.0 44-45 35.113643710813335 38.0 34.0 40.0 26.5 41.0 46-47 34.64784081928323 38.0 33.5 40.0 24.5 41.0 48-49 34.21254401214002 38.0 33.0 40.0 23.0 41.0 50-51 33.92065928625475 38.0 33.0 40.0 22.5 41.0 52-53 33.65772667698438 38.0 33.0 40.0 21.5 41.0 54-55 33.37879507190256 37.0 33.0 40.0 19.5 41.0 56-57 31.396970393968697 36.0 29.0 39.5 11.0 40.5 58-59 26.405696035208308 32.0 15.0 37.5 2.0 40.0 60-61 27.101063303850488 32.0 18.0 37.5 2.0 40.0 62-63 30.162244824256483 34.5 28.5 38.0 2.0 40.0 64-65 30.37778597459936 35.0 29.5 38.5 2.0 40.0 66-67 23.82913086498362 26.0 16.5 35.5 2.0 38.5 68-69 16.610988782119833 13.0 7.0 28.0 2.0 34.5 70-71 15.51580615233793 12.0 7.0 25.0 2.0 33.5 72-73 16.564358879600455 13.0 7.0 28.0 2.0 34.0 74-75 17.525715151255902 16.5 7.5 29.0 2.0 34.5 76-77 18.667463168649682 20.5 7.0 30.0 2.0 34.0 78-79 21.57211048598659 26.5 9.0 33.0 2.0 35.5 80-81 25.104731887965656 30.5 19.5 34.5 2.0 36.0 82-83 26.376531790772546 33.0 25.0 35.0 2.0 36.0 84-85 26.42481032967819 33.0 25.0 35.0 2.0 36.0 86-87 26.19371898288041 33.0 24.0 35.0 2.0 36.0 88-89 25.734358816488047 33.0 21.5 35.0 2.0 35.0 90-91 24.338957116520234 32.5 10.0 34.5 2.0 35.0 92-93 21.936668450176608 30.5 2.0 34.0 2.0 35.0 94-95 14.950104030288347 4.0 2.0 31.5 2.0 34.5 96-97 12.53225184375892 2.0 2.0 28.5 2.0 34.0 98-99 12.206128285088571 2.0 2.0 29.0 2.0 34.0 100-101 11.935312935870078 2.0 2.0 29.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.361350396020498 1101 2 0.25411087474881455 1101 3 0.4822625003086536 1101 4 0.26043464381670134 1101 5 0.36507050979276556 1101 6 0.4260802576537941 1101 7 0.3684475996979799 1101 8 0.4097600717635359 1101 9 0.5112589417076094 1101 10-11 0.5818271172200866 1101 12-13 0.6002832715549999 1101 14-15 0.7503923235545997 1101 16-17 0.8132245505215323 1101 18-19 0.5739093204610342 1101 20-21 0.642984374417523 1101 22-23 0.7511501472781106 1101 24-25 0.54338215536837 1101 26-27 0.61488660029935 1101 28-29 0.6997158351300854 1101 30-31 0.6804922862040073 1101 32-33 0.8079620104129646 1101 34-35 0.9142389711229697 1101 36-37 0.6732110946571126 1101 38-39 0.6828005643970556 1101 40-41 0.8652894565884921 1101 42-43 0.9283568229442167 1101 44-45 0.7866427551132773 1101 46-47 0.7824623578018333 1101 48-49 0.5884325073676138 1101 50-51 0.34928380699592054 1101 52-53 0.5607085511376582 1101 54-55 0.4775549983149219 1101 56-57 -1.4136304395431445 1101 58-59 -9.34113188475612 1101 60-61 -9.958282977136363 1101 62-63 -3.4856007387326677 1101 64-65 -4.456695077988812 1101 66-67 -8.996139211152036 1101 68-69 -4.428707697798263 1101 70-71 -4.092896658373984 1101 72-73 -3.9729695000888157 1101 74-75 -6.3543162785455465 1101 76-77 -7.533417649378123 1101 78-79 -10.463692949460103 1101 80-81 -7.332898884156059 1101 82-83 -3.638248463769589 1101 84-85 -2.8348255230968284 1101 86-87 -2.653525954901088 1101 88-89 -3.0198069655041486 1101 90-91 -9.243344056143462 1101 92-93 -16.043046367057915 1101 94-95 -10.597720101672179 1101 96-97 -8.599447803367786 1101 98-99 -8.81361474504994 1101 100-101 -8.691672491977465 1102 1 0.3672872151926114 1102 2 0.3518777592804021 1102 3 0.4269429650871537 1102 4 0.3527549052546135 1102 5 0.37017218009051334 1102 6 0.3408224508273534 1102 7 0.40257977108505116 1102 8 0.4126830928238121 1102 9 0.40942524526607116 1102 10-11 0.4017799131532769 1102 12-13 0.4373838525281286 1102 14-15 0.37284148840572584 1102 16-17 0.4288200479797766 1102 18-19 0.4113185434094717 1102 20-21 0.5440736990362609 1102 22-23 0.5674900165591481 1102 24-25 0.5980208627031587 1102 26-27 0.664069606835298 1102 28-29 0.6861809767422926 1102 30-31 0.6068811750929015 1102 32-33 0.6810920031507948 1102 34-35 0.5901648970488935 1102 36-37 0.39552409393090215 1102 38-39 0.23349954769407333 1102 40-41 0.14295830190439318 1102 42-43 0.19941709890645143 1102 44-45 0.25344558735728384 1102 46-47 0.34842096346632445 1102 48-49 -0.05848107287930304 1102 50-51 0.020489326240657135 1102 52-53 -0.20762841327773174 1102 54-55 -0.30716722390729245 1102 56-57 -2.8644837438278223 1102 58-59 -10.093020047428592 1102 60-61 -10.5194303990681 1102 62-63 -4.69926268499265 1102 64-65 -5.898842863028751 1102 66-67 -7.00745910948174 1102 68-69 -4.25378031943951 1102 70-71 -4.008110892215669 1102 72-73 -4.188510531316119 1102 74-75 -5.193441187768492 1102 76-77 -7.110805085834187 1102 78-79 -9.73390899884282 1102 80-81 -5.628786683720328 1102 82-83 -3.230296394052811 1102 84-85 -2.7786436058855024 1102 86-87 -2.6863690921559886 1102 88-89 -2.817564772330588 1102 90-91 -8.040520889839904 1102 92-93 -12.0303012690187 1102 94-95 -9.625289092231366 1102 96-97 -8.829022966766479 1102 98-99 -9.126486930961342 1102 100-101 -8.974433929885963 1103 1 0.2124409593612313 1103 2 0.2946097644034573 1103 3 0.2798688764987638 1103 4 0.25119771909046307 1103 5 0.13639105501061977 1103 6 0.2210007467162498 1103 7 0.12595838408677906 1103 8 0.25344450395589035 1103 9 0.3625945467820131 1103 10-11 0.4423722074515979 1103 12-13 0.38097570589147267 1103 14-15 0.5775514347678126 1103 16-17 0.5314693114782187 1103 18-19 0.49745151358454365 1103 20-21 0.34744872850283315 1103 22-23 0.4914467498765731 1103 24-25 0.4366419123686498 1103 26-27 0.4533051877358574 1103 28-29 0.35465017160301926 1103 30-31 0.5098854888484183 1103 32-33 0.5116988968514278 1103 34-35 0.6681570863436335 1103 36-37 0.6046664128284931 1103 38-39 0.4353929655507969 1103 40-41 0.6100848722660501 1103 42-43 0.6526146783185354 1103 44-45 0.5499678565758757 1103 46-47 0.7746344230441977 1103 48-49 0.7135748102493977 1103 50-51 0.7817505604481951 1103 52-53 0.8871628483018128 1103 54-55 0.632976227607621 1103 56-57 -2.129811489649075 1103 58-59 -9.710605045028725 1103 60-61 -10.015068846396389 1103 62-63 -3.745758803413473 1103 64-65 -4.519202097413473 1103 66-67 -5.509095473755092 1103 68-69 -3.2178218464058634 1103 70-71 -3.539032146032559 1103 72-73 -3.853391686575783 1103 74-75 -4.458688103851209 1103 76-77 -6.1377023700050195 1103 78-79 -8.696293391045728 1103 80-81 -4.368568728707707 1103 82-83 -2.062878986780536 1103 84-85 -1.6361920774527015 1103 86-87 -1.580146803868594 1103 88-89 -1.7435099672653855 1103 90-91 -4.5712331875215675 1103 92-93 -9.44390528938479 1103 94-95 -8.122899735130586 1103 96-97 -7.661962161281868 1103 98-99 -7.793088032948452 1103 100-101 -7.94782693208263 1104 1 0.0978470462543477 1104 2 0.20614917584854808 1104 3 0.07323393249168575 1104 4 0.1593443897400988 1104 5 0.10740080937561913 1104 6 0.11370508384516853 1104 7 0.07005295932381728 1104 8 0.15686852834298293 1104 9 0.18252995674995276 1104 10-11 0.181792355495908 1104 12-13 0.2053790243043423 1104 14-15 0.3124688625509293 1104 16-17 0.34546130946477405 1104 18-19 0.1859163360089937 1104 20-21 0.17794834863065034 1104 22-23 0.28570962142802614 1104 24-25 0.44749825952713707 1104 26-27 0.36181808810113836 1104 28-29 0.19585254351082426 1104 30-31 0.3111410663835059 1104 32-33 0.3419400157220167 1104 34-35 0.2436347491535571 1104 36-37 0.18069118566963027 1104 38-39 0.2505401042782367 1104 40-41 0.02030778549760015 1104 42-43 0.008972421477899672 1104 44-45 -0.03454135561861449 1104 46-47 -0.13121796074196368 1104 48-49 -0.015991712073670783 1104 50-51 -0.23297507304452836 1104 52-53 -0.09982741144608553 1104 54-55 -0.16836049909374395 1104 56-57 -3.215283201520137 1104 58-59 -10.2146434509298 1104 60-61 -10.052961273179847 1104 62-63 -4.326188211871333 1104 64-65 -5.489290514722473 1104 66-67 -6.738904032287163 1104 68-69 -4.18945539088384 1104 70-71 -4.136690110529212 1104 72-73 -4.5254660239163975 1104 74-75 -4.924866777850312 1104 76-77 -6.501972509699193 1104 78-79 -9.057171966019325 1104 80-81 -5.415488000576232 1104 82-83 -3.3275734542891158 1104 84-85 -2.8174652020249056 1104 86-87 -2.7721424382786637 1104 88-89 -2.693361193793873 1104 90-91 -4.863605401106174 1104 92-93 -6.770659816685015 1104 94-95 -7.353634335018153 1104 96-97 -7.427291944437183 1104 98-99 -7.758928232598922 1104 100-101 -7.747283350276037 1105 1 0.2688866334230795 1105 2 0.25364442131800047 1105 3 0.2703828516401039 1105 4 0.21278975766941954 1105 5 0.28572202148265546 1105 6 0.19762342522163578 1105 7 0.10142046289702478 1105 8 0.2063540097728236 1105 9 0.1651815929400584 1105 10-11 0.3836237562168634 1105 12-13 0.22857224421511546 1105 14-15 0.5372710202773661 1105 16-17 0.5730822663593713 1105 18-19 0.42082406419722673 1105 20-21 0.4531206930528171 1105 22-23 0.36678998176647326 1105 24-25 0.4798337457524511 1105 26-27 0.3904818837253359 1105 28-29 0.3535188585098723 1105 30-31 0.5403718967947242 1105 32-33 0.5452554381793107 1105 34-35 0.6681863729456268 1105 36-37 0.5722572449883927 1105 38-39 0.4673387437690266 1105 40-41 0.515294533632634 1105 42-43 0.5761686169177622 1105 44-45 0.7838145242093049 1105 46-47 0.814750454671838 1105 48-49 0.6518143264725325 1105 50-51 0.761355748711452 1105 52-53 0.6979664395949996 1105 54-55 0.49708733908612857 1105 56-57 1.6878361915842142 1105 58-59 -8.057532006605175 1105 60-61 -9.655825687770232 1105 62-63 -3.5499119742313603 1105 64-65 -4.795880656644915 1105 66-67 -6.077506141824998 1105 68-69 -3.580768343090572 1105 70-71 -3.6305112091825045 1105 72-73 -2.1208307952427763 1105 74-75 -3.8411229447045088 1105 76-77 -6.020168150736232 1105 78-79 -8.608586394996113 1105 80-81 -4.168426452505763 1105 82-83 -1.8296086038960055 1105 84-85 -1.2479379415468088 1105 86-87 -1.148157615348378 1105 88-89 -1.2340737768665768 1105 90-91 -3.7633707740882762 1105 92-93 -6.057072970769234 1105 94-95 -7.6058728826231095 1105 96-97 -7.679325573529997 1105 98-99 -7.833705243361823 1105 100-101 -7.713205822772277 1106 1 0.09512048686605823 1106 2 0.13068320179283788 1106 3 0.19922616208363308 1106 4 0.03812507009203614 1106 5 -0.0062956677054870624 1106 6 0.0625582450751736 1106 7 0.21750420347156307 1106 8 0.055864194754448704 1106 9 0.07228270131627568 1106 10-11 0.08128553790492532 1106 12-13 0.01699361397289323 1106 14-15 0.266875552066125 1106 16-17 0.24440903689470161 1106 18-19 0.23434782500330442 1106 20-21 0.20720519901570356 1106 22-23 0.19231017523058824 1106 24-25 0.23448103726914127 1106 26-27 0.3302429944293266 1106 28-29 0.37884756119577645 1106 30-31 0.19947376768549674 1106 32-33 0.3124344073381877 1106 34-35 0.2879286985862777 1106 36-37 0.29646406460820174 1106 38-39 0.2762229962628808 1106 40-41 0.10396870187150142 1106 42-43 0.007497284160145057 1106 44-45 0.23081640990014307 1106 46-47 -0.05822597203743385 1106 48-49 0.07662258423692947 1106 50-51 0.08390994256544815 1106 52-53 -0.0014857186037815495 1106 54-55 0.06390195848263858 1106 56-57 1.0839411886888648 1106 58-59 -8.128727971408807 1106 60-61 -9.346892341217426 1106 62-63 -3.663369781360199 1106 64-65 -5.328995916563233 1106 66-67 -6.654428868734147 1106 68-69 -4.000597984311192 1106 70-71 -3.9477184613089946 1106 72-73 -4.701760555294964 1106 74-75 -5.235815230327516 1106 76-77 -6.448817719259324 1106 78-79 -8.96579812066905 1106 80-81 -4.912956536147675 1106 82-83 -2.5772771150623903 1106 84-85 -2.0187266411960643 1106 86-87 -1.9513421673399414 1106 88-89 -1.8816430940855646 1106 90-91 -4.106997095975746 1106 92-93 -6.220090392215148 1106 94-95 -6.757145328786086 1106 96-97 -6.887969815946402 1106 98-99 -6.153569322268668 1106 100-101 -6.812453414409331 1107 1 0.17497689718105747 1107 2 0.005435428185084135 1107 3 0.12955536538080636 1107 4 0.06875283939648114 1107 5 0.05965876289599947 1107 6 0.09448272006170555 1107 7 0.17026917604271574 1107 8 0.14794505511200384 1107 9 0.2166959181936292 1107 10-11 0.22778281406134226 1107 12-13 0.16149656250262012 1107 14-15 0.18985770855813655 1107 16-17 0.20407882441396197 1107 18-19 0.28682560868386275 1107 20-21 0.1743800752955167 1107 22-23 0.22736621348055053 1107 24-25 0.3854487110248428 1107 26-27 0.2910345466107813 1107 28-29 0.34383103343466104 1107 30-31 0.30656075930927074 1107 32-33 0.23664805340418127 1107 34-35 0.319615396594763 1107 36-37 0.26335641709125923 1107 38-39 0.10465821905443562 1107 40-41 0.0996042209441228 1107 42-43 0.1899876635941311 1107 44-45 0.2742184438810682 1107 46-47 0.2624578164757665 1107 48-49 0.13171639070598928 1107 50-51 0.03942660647114593 1107 52-53 0.04271985399364553 1107 54-55 -0.017947827399069638 1107 56-57 0.8161707512934662 1107 58-59 -8.642683504495746 1107 60-61 -10.057021497673247 1107 62-63 -3.9268649934505966 1107 64-65 -5.581315372670414 1107 66-67 -6.992984251014786 1107 68-69 -3.7211564817320593 1107 70-71 -3.6755235632079746 1107 72-73 -2.3135664321707594 1107 74-75 -4.570024220820244 1107 76-77 -6.478625288897751 1107 78-79 -7.356213637723297 1107 80-81 -2.5529072099205123 1107 82-83 -1.2911397746990438 1107 84-85 -1.107465295021342 1107 86-87 -0.9977534248778746 1107 88-89 -0.8834498658977346 1107 90-91 -0.6467260825840704 1107 92-93 -3.18435981947491 1107 94-95 -5.80032505676351 1107 96-97 -6.408485042241537 1107 98-99 -5.922722626773625 1107 100-101 -6.538911113432709 1108 1 -0.08791010609235528 1108 2 -0.08516951302150844 1108 3 -0.10752745743432612 1108 4 -0.07191062091111178 1108 5 -0.03021286082617536 1108 6 -0.09780738838150427 1108 7 -0.04964834859931244 1108 8 -0.014382359258092947 1108 9 -0.16022378262746173 1108 10-11 -0.17573564688629517 1108 12-13 -0.19774991263589925 1108 14-15 -0.24140794987319225 1108 16-17 -0.11376401522542068 1108 18-19 0.036446606068786025 1108 20-21 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10.635448313524131 2316 96-97 12.152161638692299 2316 98-99 12.106393997598287 2316 100-101 10.888229351926526 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 33.0 5 107.0 6 259.0 7 529.0 8 1127.0 9 2014.0 10 3292.0 11 5124.0 12 7412.0 13 10302.0 14 12307.0 15 16448.0 16 21928.0 17 29052.0 18 30270.0 19 27315.0 20 26962.0 21 26601.0 22 26296.0 23 23088.0 24 23032.0 25 26280.0 26 36580.0 27 54172.0 28 75903.0 29 100887.0 30 123087.0 31 132506.0 32 131102.0 33 119370.0 34 104832.0 35 92686.0 36 72319.0 37 45183.0 38 16544.0 39 1068.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.59135685046616 20.537720841364564 15.216204993532928 20.654717314636343 2 14.94621815973396 23.289521482580685 39.458086070580514 22.30617428710484 3 19.382816302087058 28.09233492236467 32.10109773594591 20.423751039602365 4 11.876282847379468 18.225741868842597 39.35675832224776 30.541216961530182 5 11.471760232710682 40.23494396431245 34.47680126749228 13.816494535484585 6 27.421430309524293 38.99975245910491 18.159473838854385 15.41934339251641 7 24.545397808035894 36.25120702483193 21.985348103507157 17.218047063625022 8 24.94984267324351 33.74022536961444 21.053875385427762 20.256056571714286 9 25.99438860554534 17.288732962279116 22.02216367572283 34.69471475645272 10-11 23.561931155581206 27.27820020238046 28.292451685697394 20.86741695634094 12-13 26.702474777826783 25.604108477609472 28.26289404057567 19.430522703988075 14-15 22.76965238480761 26.343383824801464 29.291912120231427 21.595051670159496 16-17 19.501383914890813 29.744422791433824 29.583661459425382 21.170531834249985 18-19 19.228947328745054 28.634080270009388 32.72009001247875 19.416882388766812 20-21 21.135784946568332 26.814604492060813 31.91790898769799 20.13170157367287 22-23 20.265704082509217 27.2092130383637 31.806826329879083 20.718256549247997 24-25 20.697854949450466 27.219470599084026 32.26972560828091 19.812948843184596 26-27 20.785836679180502 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30.938284138411355 20.334170215693792 52-53 20.453221382104463 28.90403237806984 30.159412578930674 20.483333660895028 54-55 19.662488419752393 28.05072850284944 30.71467673563347 21.572106341764687 56-57 20.018263681013178 28.748726302168727 30.34581116482091 20.887198851997194 58-59 20.26346886545772 27.71754576283742 31.15965049365243 20.85933487805243 60-61 20.699938818262044 27.997235544052263 30.73328568488304 20.56953995280266 62-63 20.856482950602732 28.1038837358284 30.246791721644268 20.792841591924596 64-65 20.075438978326652 28.797715417073288 30.616465285432447 20.51038031916761 66-67 20.739957263430217 27.89211517434091 30.068392804819656 21.29953475740922 68-69 21.31346599077383 27.839298395524324 29.92925930844154 20.917976305260304 70-71 21.406706661345577 27.870782650514496 29.59245732224557 21.13005336589435 72-73 20.742845501122677 27.38539087791945 30.334729690135493 21.537033930822385 74-75 20.58231444937343 28.53197009986029 29.752198550205204 21.133516900561077 76-77 18.766205412447746 28.488563939108186 30.712945697734124 22.032284950709943 78-79 19.244207712291868 28.358560737403906 30.63990937457201 21.75732217573222 80-81 19.958738091420983 29.017414405745374 29.468867816307682 21.554979686525964 82-83 20.104486429912573 28.817883308292817 29.26167013493826 21.815960126856343 84-85 19.984761827290423 28.58104071179728 28.920866689480683 22.513330771431615 86-87 20.081092661198312 28.748940234484298 29.064783667584603 22.105183436732787 88-89 20.414109585150594 28.14992179301393 28.94198447641291 22.49398414542257 90-91 19.593114296007943 28.170894807602746 29.66037205296966 22.575618843419655 92-93 18.657765037251256 29.025638354116083 30.24388080442721 22.072715804205455 94-95 18.75591066014831 28.680168367076853 29.69916259984634 22.8647583729285 96-97 18.718365121323835 28.592021374231162 29.153245809046474 23.53636769539853 98-99 18.64909518160378 28.64117926527779 29.06229463424626 23.64743091887217 100-101 19.03941347823671 28.466272910563994 29.136005113237616 23.358308497961676 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2889.0 1 2157.0 2 1417.0 3 1449.0 4 1521.0 5 1778.0 6 2296.0 7 3113.0 8 3899.0 9 4323.5 10 4468.5 11 4549.5 12 4713.0 13 4959.5 14 5204.0 15 5318.5 16 5464.5 17 5619.0 18 5766.5 19 5955.0 20 6089.0 21 6393.5 22 7060.0 23 7770.5 24 8724.5 25 10162.0 26 11841.0 27 13863.0 28 16026.5 29 18306.5 30 20884.5 31 23676.0 32 26679.5 33 29917.0 34 33543.0 35 36733.0 36 39345.5 37 41403.0 38 42427.0 39 42937.5 40 43583.5 41 44483.5 42 45599.5 43 47165.0 44 49436.5 45 52884.5 46 57566.5 47 62801.5 48 67195.0 49 63787.5 50 54361.0 51 47004.0 52 41024.0 53 36206.0 54 32145.0 55 28291.5 56 24903.0 57 21819.0 58 18774.0 59 15834.5 60 13239.0 61 10589.5 62 8158.0 63 6105.0 64 4337.5 65 3078.0 66 2159.5 67 1434.5 68 929.0 69 587.5 70 393.0 71 281.5 72 219.0 73 171.0 74 114.5 75 78.5 76 47.0 77 18.5 78 10.0 79 5.5 80 2.0 81 1.0 82 1.0 83 0.5 84 0.5 85 0.5 86 0.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024052840514239913 2 0.005680116856132457 3 7.01248994584254E-5 4 0.0 5 0.14817391255565288 6 0.0 7 0.0 8 9.817485924179556E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 7.363114443134667E-4 16-17 0.0 18-19 1.753122486460635E-4 20-21 0.0 22-23 3.155620475629143E-4 24-25 0.0 26-27 3.856869470213397E-4 28-29 0.003751682121025759 30-31 0.01248223210359972 32-33 0.0029101833275246543 34-35 0.004698368263714502 36-37 0.1165125204501738 38-39 0.11644239555071538 40-41 0.12895969010404432 42-43 0.1311335619872555 44-45 0.12734681741650053 46-47 0.11998370297336587 48-49 0.12079013931713775 50-51 0.1187214547831142 52-53 0.09529973836400012 54-55 0.08323825565715094 56-57 0.5548282045150618 58-59 1.550040777629035 60-61 0.5121221407448807 62-63 0.004733430713443714 64-65 0.1190019543809479 66-67 1.8763319348090883 68-69 2.8332563128187616 70-71 2.741357632078495 72-73 2.5901332863964006 74-75 1.9756989173416772 76-77 1.392750628143787 78-79 0.9241409875128592 80-81 0.051997612948422436 82-83 3.155620475629143E-4 84-85 2.1037469837527622E-4 86-87 2.804995978337016E-4 88-89 2.454371481044889E-4 90-91 0.24645395914663606 92-93 0.38835169320075985 94-95 1.9725432968660483 96-97 2.389190386998283 98-99 1.4024278642690495 100-101 0.9272966079884882 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1426027.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.09282578678208 #Duplication Level Percentage of deduplicated Percentage of total 1 70.62939282311208 29.729907275294533 2 14.020314757701147 11.803093331435283 3 5.857660190260161 7.396964097203691 4 2.8967761742766616 4.8773397938851435 5 1.7085535874748286 3.5958924252479747 6 1.066817042985264 2.6943206362049232 7 0.7024038654514989 2.069631447828858 8 0.5116178693198616 1.7228353474148461 9 0.38345663322171847 1.452669593308901 >10 2.0198069346456613 15.227356187296587 >50 0.1295796031014788 3.7260438744261806 >100 0.06362680172378773 4.875499482659661 >500 0.002664991126920212 0.8078146733061983 >1k 0.00616279198100299 5.520651189505004 >5k 8.328097271625663E-4 2.6069936479966733 >10k+ 3.331238908650265E-4 1.8929869969855364 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15383 1.0787313283689581 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11617 0.814640957008528 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9539 0.6689214159339198 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9404 0.6594545545070325 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6932 0.4861058030458049 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5848 0.4100904120328717 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5461 0.3829520759424611 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4633 0.3248886591908849 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4441 0.3114246784948672 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4199 0.29445445282592825 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4065 0.28505771629849924 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3452 0.2420711529304845 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3046 0.2136004437503638 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2851 0.1999260883559708 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2758 0.19340447270633726 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2689 0.18856585464370593 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2621 0.183797361480533 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2553 0.17902886831736006 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2518 0.17657449683631518 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2476 0.1736292510590613 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2365 0.16584538721917608 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2109 0.1478934129578192 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2013 0.14116142260981035 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1939 0.13597218004988684 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1920 0.13463980696017677 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1870 0.1311335619872555 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1809 0.12685594312029155 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1677 0.1175994563917794 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1535 0.10764172066868298 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1531 0.10736122107084928 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1500 0.1051873491876381 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1486 0.10420560059522013 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1452 0.1018213540136337 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0518734918763811E-4 0.0 12-13 0.0 0.0 0.0 2.1037469837527622E-4 0.0 14-15 0.0 0.0 0.0 2.1037469837527622E-4 0.0 16-17 0.0 0.0 0.0 3.5062449729212703E-4 0.0 18-19 0.0 0.0 0.0 4.908742962089778E-4 0.0 20-21 0.0 0.0 0.0 9.817485924179556E-4 0.0 22-23 0.0 0.0 0.0 0.0021738718832111873 0.0 24-25 0.0 0.0 0.0 0.0031906829253583557 0.0 26-27 0.0 0.0 0.0 0.004908742962089778 0.0 28-29 0.0 0.0 0.0 0.012271857405224445 0.0 30-31 0.0 0.0 0.0 0.030819893311977965 0.0 32-33 0.0 0.0 0.0 0.06619790508875359 0.0 34-35 0.0 0.0 0.0 0.11805526823825917 0.0 36-37 0.0 0.0 0.0 0.18979304038422834 0.0 38-39 0.0 0.0 0.0 0.28800296207575315 0.0 40-41 0.0 0.0 0.0 0.39326043616284967 0.0 42-43 0.0 0.0 0.0 0.5002359702866777 0.0 44-45 0.0 0.0 0.0 0.6281087244491164 0.0 46-47 0.0 0.0 0.0 0.7720050181378053 0.0 48-49 0.0 0.0 0.0 0.9025425184796642 0.0 50-51 0.0 3.50624497292127E-5 0.0 1.0261025913254098 0.0 52-53 0.0 7.01248994584254E-5 0.0 1.1403360525431847 0.0 54-55 0.0 7.01248994584254E-5 0.0 1.2655791229759323 0.0 56-57 0.0 7.01248994584254E-5 0.0 1.3705560974651954 0.0 58-59 0.0 7.01248994584254E-5 0.0 1.442539306759269 0.0 60-61 0.0 7.01248994584254E-5 0.0 1.5062828403669777 0.0 62-63 1.0518734918763811E-4 7.01248994584254E-5 0.0 1.5603842002991528 0.0 64-65 1.402497989168508E-4 7.01248994584254E-5 0.0 1.6087703809254663 0.0 66-67 1.402497989168508E-4 7.01248994584254E-5 0.0 1.6487415736167688 0.0 68-69 1.402497989168508E-4 7.01248994584254E-5 0.0 1.7073309271142834 0.0 70-71 1.402497989168508E-4 7.01248994584254E-5 0.0 1.8056460361549957 0.0 72-73 1.402497989168508E-4 7.01248994584254E-5 0.0 1.9414078415065072 0.0 74-75 1.402497989168508E-4 7.01248994584254E-5 0.0 2.112793095782899 0.0 76-77 1.402497989168508E-4 7.01248994584254E-5 0.0 2.371624099683947 0.0 78-79 1.402497989168508E-4 7.01248994584254E-5 0.0 2.7860973179329704 0.0 80-81 1.402497989168508E-4 7.01248994584254E-5 0.0 3.2890330968487973 0.0 82-83 1.402497989168508E-4 7.01248994584254E-5 0.0 3.7725442786146406 0.0 84-85 1.402497989168508E-4 1.0518734918763811E-4 0.0 4.165419027830469 0.0 86-87 1.402497989168508E-4 1.402497989168508E-4 0.0 4.463099226031485 0.0 88-89 1.402497989168508E-4 1.402497989168508E-4 0.0 4.7315373411583375 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8375 0.0 26.137768 1 TACATGG 8350 0.0 25.930487 2 ACATGGG 8370 0.0 24.1789 3 AGTACTT 9675 0.0 23.434416 12-13 GCTTACG 425 0.0 23.167496 60-61 CTTTGCG 520 0.0 22.629553 2 GAGTACT 9530 0.0 22.607588 12-13 TTACGCG 155 7.2759576E-12 21.956627 62-63 CATGGGA 6325 0.0 21.430166 4 CATGGGG 2015 0.0 21.021372 4 GTACTTT 10555 0.0 20.412157 14-15 TTGCGTT 605 0.0 20.226072 4 ATGGGAG 2180 0.0 20.07798 5 TACTTTT 11485 0.0 19.598024 14-15 CATGGGT 705 0.0 19.35987 4 ATGGGAT 2165 0.0 18.91276 5 TTTGCGT 625 0.0 18.825806 3 ACTTTTT 11800 0.0 18.457611 16-17 AGTACAT 6185 0.0 16.970884 2 ATGGGGA 990 0.0 16.638968 5 >>END_MODULE