FastQCFastQC Report
Fri 27 May 2016
SRR939564_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939564_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356802
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63210.46587490289666433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43600.3213438659435938No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37390.2755744758630957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33780.24896779338473854No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30520.2249407061605157No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23170.17076920582369423No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19540.14401511790224367No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18240.1344337641011732No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16980.12514722118628951No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15490.11416551567583184No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG65050.035.502671
CGCATAC1250.003677696232.1314621
TACATGG81500.029.4414462
ACATGGG81300.028.351913
GTACCGT1057.214016E-526.9698286
CATGGGA63250.025.9103854
GAGTACT85900.024.56057712-13
CATGGGG18250.024.0671674
ATGGGAT21600.023.5985985
ATGGGAG24050.022.9607985
GCACTAG2200.002084814222.820641
GCTTATA8000.022.5924341
AGTACTT91750.022.04291212-13
GTACTTT96450.021.88012314-15
GGTCGGG3855.7154775E-620.8645861
GGGACCG1708.355783E-519.4341417
GTGCTAC3658.955491E-519.256871
ACTTTTT116350.019.05398816-17
GTATAGC3709.941391E-518.9966411
GTATATG4301.4848525E-518.6810821
Produced by FastQC (version 0.11.5)