FastQCFastQC Report
Fri 27 May 2016
SRR939561_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939561_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838206
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143410.7801628326749015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96090.522737930351658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75490.41067214447129435No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25490.13866780981021712No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25350.13790619767316611No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19960.108584130396702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53550.052.461421
GTATCAA100000.041.9972721
ATCAACG128200.032.7221683
TATCAAC128500.032.5349852
TCAACGC129550.032.417814
CAACGCA131950.031.8281735
AACGCAG135450.031.1057626
CGTATAG1354.2629836E-728.1212141
ACGCAGA150300.027.5596687
CGCAGAG150750.027.2578188
GTACATG89400.026.3282491
TACATGG90500.025.9033662
GCAGAGT153050.025.0500479
ACATGGG91000.024.3008983
GAGTACT88400.023.91255212-13
AGTACTT92600.022.52052112-13
CATGGGA67100.022.1360244
AGAGTAC138600.021.567910-11
GTACTTT99900.021.1847614-15
CAGAGTA146050.021.18246810-11
Produced by FastQC (version 0.11.5)