FastQCFastQC Report
Fri 27 May 2016
SRR939561_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939561_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838206
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146990.7996383430366346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111890.6086913001045585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92660.5040784329939082No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29710.16162497565561204No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28340.15417205688589855No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24260.13197650317755466No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18680.10162081942937842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55900.053.5330281
GTATCAA98800.040.05121
ATCAACG129600.030.3092483
TCAACGC130800.029.9952164
TATCAAC131350.029.9786952
CAACGCA133000.029.4636865
AACGCAG136000.028.7753456
ACGCAGA155050.025.0581047
CGCAGAG154700.024.6903068
GTACATG85100.024.106461
TACATGG85450.023.2887232
GCAGAGT161100.022.8630929
ACATGGG84000.022.4013613
GAGTACT100500.021.66696712-13
AGTACTT103350.021.02401712-13
AGAGTAC146050.020.63524210-11
CATGGGA59700.020.1724824
CAGAGTA154650.020.09529510-11
GTACTTT110300.019.82680514-15
CATGGGG28750.018.8171444
Produced by FastQC (version 0.11.5)